全文获取类型
收费全文 | 15494篇 |
免费 | 1483篇 |
国内免费 | 2820篇 |
出版年
2024年 | 67篇 |
2023年 | 398篇 |
2022年 | 368篇 |
2021年 | 518篇 |
2020年 | 619篇 |
2019年 | 694篇 |
2018年 | 553篇 |
2017年 | 604篇 |
2016年 | 659篇 |
2015年 | 616篇 |
2014年 | 642篇 |
2013年 | 979篇 |
2012年 | 612篇 |
2011年 | 700篇 |
2010年 | 513篇 |
2009年 | 688篇 |
2008年 | 676篇 |
2007年 | 761篇 |
2006年 | 735篇 |
2005年 | 740篇 |
2004年 | 576篇 |
2003年 | 585篇 |
2002年 | 508篇 |
2001年 | 458篇 |
2000年 | 430篇 |
1999年 | 423篇 |
1998年 | 370篇 |
1997年 | 320篇 |
1996年 | 339篇 |
1995年 | 320篇 |
1994年 | 283篇 |
1993年 | 313篇 |
1992年 | 322篇 |
1991年 | 268篇 |
1990年 | 263篇 |
1989年 | 257篇 |
1988年 | 240篇 |
1987年 | 197篇 |
1986年 | 190篇 |
1985年 | 172篇 |
1984年 | 163篇 |
1983年 | 81篇 |
1982年 | 115篇 |
1981年 | 110篇 |
1980年 | 82篇 |
1979年 | 79篇 |
1978年 | 47篇 |
1977年 | 32篇 |
1976年 | 33篇 |
1975年 | 21篇 |
排序方式: 共有10000条查询结果,搜索用时 968 毫秒
61.
62.
G. Van den Eede B. Dreyfus K. Goethals M. Van Montagu M. Holsters 《Molecular & general genetics : MGG》1987,206(2):291-299
Summary After random Tn5 mutagenesis of the stem-nodulating Sesbania rostrata symbiont strain ORS571, Nif-, Fix- and Nod- mutants were isolated. The Nif- mutants had lost both free-living and symbiotic N2 fixation capacity. The Fix- mutants normally fixed N2 in the free-living state but induced ineffective nodules on S. rostrata. They were defective in functions exclusively required for symbiotic N2 fixation. A further analysis of the Nod- mutants allowed the identification of two nod loci. A Tn5 insertion in nod locus 1 completely abolished both root and stem nodulation capacity. Root hair curling, which is an initial event in S. rostrata root nodulation, was no longer observed. A 400 bp region showing weak homology to the nodC gene of Rhizobium meliloti was located 1.5 kb away from this nod Tn5 insertion. A Tn5 insertion in nod locus 2 caused the loss of stem and root nodulation capacity but root hair curling still occurred. The physical maps of a 20.5 kb DNA region of nod locus 1 and of a 40 kb DNA region of nod locus 2 showed no overlaps. The two nod loci are not closely linked to nif locus 1, containing the structural genes for the nitrogenase complex (Elmerich et al. 1982). 相似文献
63.
A sand-culture experiment was conducted to study the influence of a deficiency of and an excess of micronutrients on the uptake
and assimilation of NH
4
+
and NO
3
−
ions by maize. By studying the fate of15N supplied as15NH4NO3 or NH4
15NO3, it was demonstrated that in maize plants NH4−N was absorbed in preference to NO
3
−
−N. The uptake and distribution of N originating from both NH
4
+
and NO
3
−
was considerably modified by deficiency of, or an excess of, micronutrients in the growth medium. The translocation of NH
4
+
−N from roots to shoots was relatively less than that of NO
3
−
−N. Deficiency as well as excessive amounts of micronutrients, in the growth medium, substantially reduced the translocation
of absorbed N into protein. This effect was more pronounced in the case of N supplied as NO
3
−
. Amino-N was the predominant non-protein fraction in which N from both NH
4
+
and NO
3
−
tended to accumulate. The next important non-protein fractions were NO
3
−
−N when N was supplied as NO
3
−
and amide-N when NH
4
+
was the source. The relative accumulation of15N into different protein fractions was also a function of imposed micronutrient levels. 相似文献
64.
Growth of 2-month-old nonnodulatedHippophaë rhamnoides seedlings supplied with combined N was compared with that of nodulated seedlings grown on zero N. Plant growth was significantly better with combined N than with N2 fixation and, although not statistically significant for individual harvests, tended to be highest in the presence of NH 4 + , a mixture of NH 4 + and NO 3 ? producing the highest yields. Growth was severely reduced when solely dependent on N2 fixation and, unlike the combined-N plants, shoot to root ratios had only slightly increased after an initial decrease. An apparently insufficient nodule mass (nodule weight ratio <5 per cent) during the greater part of the experimental period is suggested as the main cause of the growth reduction in N2-fixing plants. Thein vivo nitrate reductase activity (NRA) of NO 3 ? dependent plants was almost entirely located in the roots. However, when grown with a combination of NO 3 ? and NH 4 + , root NRA was decreased by approximately 85 per cent.H. rhamnoides demonstrated in the mixed supply a strong preference for uptake of N as NH 4 + , NO 3 ? contributing only for approximately 20 per cent to the total N assimilation. Specific rates of N acquisition and ion uptake were generally highest in NO 3 ? +NH 4 + plants. The generation of organic anions per unit total plant dry weight was approximately 40 per cent less in the NH 4 + plants than in the NO 3 ? plants. Measured extrusions of H+ or OH? (HCO 3 ? ) were generally in good agreement with calculated values on the basis of plant composition, and the acidity generated with N2 fixation amounted to 0.45–0.55 meq H+. (mmol Norg)?1. Without acidity control and in the presence of NH 4 + , specific rates of ion uptake and carboxylate generation were strongly depressed and growth was reduced by 30–35 per cent. Growth of nonnodulatedH. rhamnoides plants ceased at the lower pH limit of 3.1–3.2 and deterioration set in; in the case of N2-fixing plants the nutrient solution pH stabilized at a value of 3.8–3.9 without any apparent adverse effects upon plant performance. The chemical composition of experimental and field-growing plants is being compared and some comments are made on the nitrogen supply characteristics of their natural sites. 相似文献
65.
J. H. Becking 《Plant and Soil》1987,100(1-3):183-212
Summary The survival of Azolla was studied in an artificial system which simulated the soil/water interface and the desiccation of
soil during a fallow period in lowland rice culture. Tests with non-sporulating and sporulating Azolla fronds showed that
Azolla only survives with sporulated fronds. At their reappearance the Azolla fronds already harboured the Anabaena endophyte.
A detailed light microscopic and transmission electron microscopic study of macro- and micros-porocarp formation and development
revealed that the endophyte is transmitted by the macrosporocarps and not by the microsporocarps. The Anabaena cells within
the macrosporocarps are found just below the indusium cap. These cells are not nitrogen-fixing akinetes. The free-living Anabaena
cells at the stem apex and below the overarching developing leaves do not bear heterocysts and accordingly are non nitrogen-fixing.
During the development of the leaf the Anabaena enters the leaf cavity, but later the pore of this, cavity closes and the
imprisoned cyanobacteria are lysed before the leaf decays. As the Azolla leaves age a nitrogen-fixing capability is successively
built up concomittantly with the production of heterocysts. Heterocyst frequencies of 40–50% can be found inAnabaena azollae. Usually a gradient of nitrogen-fixing capacity occurs along the Azolla rhizome with two distinct peaks at leaf number 7/8
and at leaf number 13/14 from the apex. 相似文献
66.
Model of ammonia volatilization from calcareous soils 总被引:2,自引:0,他引:2
A quantitative model of ammonia volatilization from the calcareous soil uppermost 1-cm layer was developed and tested. The
model accounts for the following processes: ammonium-ammonia equilibration in the soil solution, cation exchange between calcium
and ammonium which results in ammonium distribution between soil liquid and solid phases, nitrification of dissolved ammonium,
distribution of ammonia between liquid and gaseous phases and diffusion of gaseous ammonia in the soil air.
The combined effect of various characteristics such as soil pH, cation exchange capacity, water capacity and nitrification
rate on ammonia losses from various soil types have been studied. The model was validated against experimental results of
ammonia losses from different soils for its use as a predicting tool.
The model shows that most of ammonia losses can be explained by the interactive effect of high soil pH and low cation exchange
capacity. Computations show increased ammonia volatilization with decreasing soil water capacity. Increasing fertilizer application
rate has a small effect on percentage of ammonia losses. Increased nitrification rate and shorter “lag” period of nitrification
reduce ammonia losses considerably. Good agreement was obtained between model calculations and experimental results of ammonia
volatilization from 13 soils. 相似文献
67.
Summary The alder has a perennial nodule cluster. The nodule amount on the roots increases with tree age. The N2-fixing activity of nodules decreases with nodule age. Purple coloured soils with various soil pHs and CaCO3 contents are, in the main, the ones which influence nodulation and N2-fixing. Higher N2-fixing capacity existed in the neutral and low calcium soils. High calcium soils and acid soils can restrain nodulation and
the N2-fixing rate significantly. On the slope, where calcarous light loams are found, the annual nitrogen fixation capacity of
alder and cypress mixed plantations, less than 10 years old, is 16 or 17 kg/ha yr, but in the valley, a pure alder plantation
can reach 40 kg/ha yr. 相似文献
68.
C. A. Atkins 《Plant and Soil》1987,100(1-3):157-169
Summary Nitrogen (N2) fixed by Rhizobium bacteroids in the legume nodule is excreted as ammonia to the surrounding host cell where it is efficiently
assimilated into the amide group of glutamine. Generally glutamine is a minor exported solute of nitrogen, being further metabolised
to asparagine in temperate species and to the ureides, allantoin and allantoic acid in tropical species. These solutes serve
as the principal translocated forms of nitrogen in xylem. Compartmentalisation of the pathways of nitrogen metabolism and
the role of ammonia in regulation of their activity is examined in nodules of both asparagine-forming (Lupinus albus L.) and ureide-forming (Vigna unguiculata L. Walp) symbioses. 相似文献
69.
In highly eutrophic ponds, buoyancy of the gas-vacuolate blue-green alga Anabaenopsis Elenkinii (Miller) was regulated by complex interactions between chemical and physical parameters, as well as by biological interactions
between various trophic levels. Algal buoyancy and surface bloom formation were enhanced markedly by decreased light intensity,
and to a lesser extent by decreased CO2 availability and increased availability of inorganic nitrogen. In the absence of dense populations of large-bodied Cladocera,
early season blooms of diatoms and green algae reduced light availability in the ponds thus creating conditions favorable
for increased buoyancy and bloom formation by A. Elenkinii. The appearance of blue-green algal blooms could be prevented by a reduced density of planktivorous fish, which allowed development
of dense cladoceran populations. The cladocerans limited the growth of precursory blooms of diatoms and green algae, and given
the resulting clear-water conditions, buoyancy of A. Elenkinii was reduced, and blue-green algal blooms never appeared. 相似文献
70.
In vitro pathogenicity tests demonstrated that Hirschmanniella caudacrena is pathogenic to Ceratophyllum demersum (coontail). Symptoms were chlorotic tissue, deformed stems, and, finally, death of the plant. Inoculum densities of 500 nematodes per 5-cm-long cutting in a test tube containing 50 ml of water resulted in death and decay of some of the cuttings within 8 weeks; 100 nematodes killed the plants in 12 weeks, and 50 and 25 nematodes killed them in 16 weeks. The lowest inoculum level of 10 nematodes did not seriously affect the plants at 16 weeks when the experiment was terminated. A second test conducted outdoors in glass jars containing 3 liters of water and two cuttings weighing a total of 15 g fresh weight showed damage, but results were not statistically significant. Hydrilla verticillata inoculated with H. caudacrena was not affected seriously. 相似文献