Rapid CE microbial assays for consumer products that contain active bacteria

Recent advances in high efficiency separation methods of bacteria allow their rapid identification and quantitation in some cases. A specific capillary electrophoresis (CE) technique is used to identify and quantitate Lactobacillus acidophilus in both pill and syrup health products as well as Bifidobacterium infantis in a powdered formula supplement. Cell viability can be evaluated as well. In some cases, both the living and dead bacterial cells as well as the molecular excipients can be evaluated in a single run.     

A proteomic view of Bifidobacterium infantis generated by multi-dimensional chromatography coupled with tandem mass spectrometry

Bifidobacteria are Gram-positive prokaryotes that naturally colonize the human gut where they exert several health-promoting effects. The present paper reports the use of a strong cation exchange-reversed-phase-tandem mass spectrometry strategy to catalogue the most abundantly expressed proteins of a probiotic Bifidobacterium infantis strain. A global view of the B. infantis proteome was obtained. The bimodal representation of the proteins identified by mass spectrometry provides the first theoretical two-dimensional map of protein distribution for this organism. Among the 136 proteins identified by multidimensional protein identification technology (MudPIT) analysis, 118 showed the highest similarity with the translated sequences of B. longum genome, two proteins were similar to other Bifidobacterium species and the remaining 16 were similar to different genera. Specific biological activities have been assigned to 115 identified proteins, whereas 21 have been referred to the group of hypothetical proteins. The MudPIT approach allowed us to identify high mass and basic isoelectric point proteins that are generally challenging to visualize using the traditional two-dimensional electrophoresis technique. Redundancy in peptide and protein identification using the double chromatography technique was also evaluated.     

阿胶,五味子,刺五加,枸杞对双歧杆菌生长的影响

本文选用１５种传统抗衰老中药对双歧杆菌进行了体外生长促进实验。结果发现刺五加、五味子、宁夏枸杞子及阿胶对婴儿双歧杆菌有明显的促进作用。     

脂磷壁酸的提取工艺及免疫功能的研究

目的确定婴儿双歧杆菌脂磷壁酸（LTA）的最佳提取工艺及其免疫调节作用。方法通过对不同方法提取婴儿双歧杆菌LTA的测定及对植瘤小鼠淋巴细胞的转化来探讨LTA对小鼠细胞免疫的调节作用。结果采用脱脂后水法提取效果最好,与对照组和全菌组相比,LTA使淋巴细胞转化增加。结论婴儿双歧杆菌及其LTA均有免疫功能,但LTA的效果要优于婴儿双歧杆菌。     

婴儿双歧杆菌对小鼠肉瘤S180的抑制作用

本文主要观察婴儿双歧杆菌(Bif. 189—3)对小鼠皮下移植肉瘤180(S180)的抑制作用。结果发现,该菌无论在S180移植前或移植后经皮下注射,均能明显抑制皮下移植S180的增长,并使带瘤鼠存活期显著延长。病理检查见实验组肿瘤坏死明显,肿瘤周围有大量炎症细胞浸润。提示该菌能非特异性增强肿瘤局部的免疫反应。     

新型婴儿双歧杆菌马铃薯酸奶的工艺化初步研究

目的 以马铃薯和牛奶为主要原料,利用婴儿双歧杆菌进行发酵,制备一款新型酸奶。方法 采用婴儿双歧杆菌作为发酵菌株,发酵马铃薯牛奶。进行马铃薯酸奶原料的比例、酸奶发酵的温度和时间的探索。结果 当发酵温度42℃、发酵时间20 h、马铃薯汁10%时,酸奶的感官及口味较好。结论 利用10%马铃薯汁,牛奶等可初步制备出新型马铃薯汁酸奶。