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131.
优先流研究现状及发展趋势 总被引:24,自引:0,他引:24
优先流是一种常见的土壤水分运动形式,它是土壤水运动机理研究由均质走向非均质领域的标志。但最初却没有真正的定义。综合介绍了目前国际上公认的土壤优先流定义、优先流多种表现形式及其重要特征。系统阐述了优先流的静态(内在)和动态(外在)影响因素、开展研究的基础理论以及实验研究技术,指出开展优先流研究可以有效及充分地解释早期水文学研究所困扰的不符合达西定律及对流一弥散方程等重大问题,但由于优先流运动过程具有非平衡性及区域特点,其自身类型较多,开展优先流研究同时加大了水文过程研究的难度及深度,所以长期以来没有得到充分重视,到目前为止,对于优先流运动机理尚未明确;开展优先流研究判定标准多样,但缺乏系统成形的判定标准;由于土壤本身就是异质性系统,对优先流研究需综合考虑尺度效应;虽然已有多种方法可以有效开展优先流研究,但缺少已获得国际标准认证的适用于具有快速环绕特性的优先流研究需要的现代仪器设备。同时还探讨了优先流研究的发展趋势。 相似文献
132.
133.
采用植物染色体常规压片技术,报道了中国柴胡属(Bupleurum L .)13种5变种24个居群的染色体数目,其中4种5变种为首次报道,同时报道了5种1变种的细胞核型.除天山柴胡两个居群、紫花鸭跖柴胡一个居群和北柴胡一个居群是多倍体外,其它均为二倍体.主要结论如下:(1)秦岭柴胡和细茎有柄柴胡处理为独立的种更为合适;紫花阔叶柴胡作为大叶柴胡变种的处理是合理的;黄花鸭跖柴胡处理为紫花鸭跖柴胡的变种是不合理的.(2)紫花鸭跖柴胡和北柴胡可能都是还没有被完全认识的复合类群,需要进一步研究.(3)x=7可能是柴胡属的原始基数. 相似文献
134.
Mona J. Law Gladys E. Deibler Russell E. Martenson Henry C. Krutzsch 《Journal of neurochemistry》1985,45(4):1232-1243
Rabbit myelin basic protein (BP) was subjected to partial cleavage with plasmin, and 15 cleavage products were isolated by a combination of gel filtration and ion-exchange chromatography. Their identification was achieved by amino acid analysis and tryptic peptide mapping, supplemented in some instances by carboxy-terminal analyses with carboxypeptidases A, B, and Y and amino-terminal analyses with dipeptidyl aminopeptidase I. The results showed that major plasmic cleavage sites included the Lys89-Asn90, Lys133-Ser134, and Lys153-Leu154 bonds. Cleavages also occurred at the Arg31-His32, Lys53-Arg54, and Arg25-His26 bonds, but these appeared to be less extensive. A large number of additional peptides were produced in relatively low yield. The smaller of these were isolated from heterogeneous fractions by high-voltage electrophoresis-TLC. Amino acid analysis of these peptides showed that minor cleavage sites included the Arg9-His10, Lys13-Tyr14, Lys103-Gly104, Lys137-Gly138, Lys140-Gly141, and Arg160-Ser161 bonds. In spite of a lower selectivity toward peptide bonds in BP as compared with pepsin, cathepsin D, and thrombin, plasmin has the advantage over the former proteinases in that it does not cleave at or near the Phe44-Phe45 bond. Instead it cleaves at the Arg31-His32 and Lys53-Arg54 bonds, thus preserving the entire hydrophobic sequence Ile-Leu-Asp-Ser-Ile-Gly-Arg-Phe-Phe as well as short sequences to either side. 相似文献
135.
桂西南石漠化山地土壤种子库的基本特征及植被恢复对策 总被引:3,自引:0,他引:3
采用幼苗萌发法对广西平果县龙何示范区石漠化山地的土壤种子库进行初步研究。结果表明,该地区土壤种子库共有108种植物,隶属于33科81属,其中草本81种、灌木20种、藤本7种;其土壤种子库的种子密度为64.6~339.7粒.m-2,且在不同土地利用类型区及不同季节存在较大差别。在分析土壤种子库对其植被恢复作用的基础上,认为可采取自然封育和人工造林等措施,以促进现存植被的正向演替。 相似文献
136.
137.
Kubota R Numaguchi Y Ishii M Niwa M Okumura K Naruse K Murohara T 《Biochemical and biophysical research communications》2010,402(2):396-401
Aminopeptidase A (APA; EC 3.4.11.7) is a transmembrane metalloprotease with several functions in tumor angiogenesis. To investigate the role of APA in the process of ischemia-induced angiogenesis, we evaluated the cellular angiogenic responses under hypoxic conditions and the process of perfusion recovery in the hindlimb ischemia model of APA-deficient (APA-KO; C57Bl6/J strain) mice.Western blotting of endothelial cells (ECs) isolated from the aorta of APA-KO mice revealed that the accumulation of hypoxia-inducible factor-1α (HIF-1α) protein in response to hypoxic challenge was blunted. Regarding the proteasomal ubiquitination, a proteasome inhibitor MG-132 restored the reduced accumulation of HIF-1α in ECs from APA-KO mice similar to control mice under hypoxic conditions. These were associated with decreased growth factor secretion and capillary formation in APA-KO mice. In the hindlimb ischemia model, perfusion recovery in APA-KO mice was decreased in accordance with a significantly lower capillary density at 2 weeks. Regarding vasculogenesis, no differences were observed in cell populations and distribution patterns between wild type and APA-KO mice in relation to endothelial progenitor cells.Our results suggested that Ischemia-induced angiogenesis is impaired in APA-KO mice partly through decreased HIF-1α stability by proteasomal degradation and subsequent suppression of HIF-1α-driven target protein expression such as growth factors. APA is a functional target for ischemia-induced angiogenesis. 相似文献
138.
In a previous report [Yanget al., (1987a),J. Biol Chem.
262, 7034–7040], a cyclic-AMP- and calcium-independent brain kinase which requires autophosphorylation for activity was identified as a very potent myelin basic protein (MBP) kinase. In this report, the phosphorylation sites of MBP by this autophosphorylation-dependent protein kinase (autokinase) are further determined by two-dimensional electrophoresis/thin-layer chromatography, phosphoamino acid analysis, high-performance liquid chromatography, tryptic peptide mapping, sequential manual Edman degradation, and direct peptide sequencing. Autokinase phosphorylates MBP on both threonine and serine residues. Three major tryptic phosphopeptide peaks were resolved by C18-reversed phase highper-formance liquid chromatography. Sequential manual Edman degradation together with direct sequence analysis revealed that FS(p)WGAEGQKPGFGYGGR is the phosphorylation site sequence (molar ratio 1.0) for the first major phosphopeptide peak. When mapping with bovine brain MBP sequence, we finally demonstrate Ser115, one of thein vivo phosphorylation sites in MBP, as the major site phosphorylated by autokinase, implicating a physiologically relevant role of autokinase in the regulation of brain myelin function. By using the same approach, we also identified HRDT(p)GILDSLGR (molar ratio 0.9) and TT(p)HYGSLPQK (molar ratio 0.8) as the major phosphorylation site sequences in32P-MBP phosphorylated by autokinase, further indicating that -Arg-XSer/Thr-(neutral amino acid)3-(amino acid-containing hydroxyl group such as Ser/Glu/Asp)-(neutral amino acid)2-may represent a unique consensus sequence motif specifically recognized by this autophosphorylation-dependent multisubstrate/ multifunctional protein kinase in the brain. 相似文献
139.
140.
Equilibrium measurements of the binding of central nervous system myelin basic protein to sodium dodecyl sulphate, sodium deoxycholate and lysophosphatidylcholine have been obtained by gel permeation chromatography and dialysis. This protein associates with large amounts of each of these surfactants: the apparent saturation weight ratios (surfactant/protein) being for dodecyl sulphate at ionic strengths 0.30 and 0.10, respectively, for deoxycholate (at 0.12 ionic strength) and for lysophosphatidylcholine. Binding to the ionic surfactants increases markedly close to their critical micelle concentrations. Sedimentation analysis shows that at 0.30 ionic strength in excess dodecyl sulphate the protein is monomeric. It becomes dimeric when the binding ratio falls below 1 at a free detergent concentration of approximately 0.25 mM: below this concentration much of the protein and detergent forms an insoluble complex. The amount of dodecyl sulphate bound at high concentrations and at both above-mentioned ionic strengths corresponds closely to that expected for interaction of a single polypeptide with two micelles. Variability of deoxycholate micelle size on interaction with other molecules precludes a similar analysis for this surfactant. Association was observed only with single micelles of lysophosphatidylcholine. The results provide strong evidence for dual lipid-binding sites on basic protein and indicate that lipid bilayer cross-linking by this protein may be effected by single molecules. 相似文献