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211.
《Archives of animal nutrition》2013,67(6):445-446
Abstract Schenk/Kolb: Grundriß der physiologischen Chemie, 6. überarbeitete Auflage. Erschienen im VEB Gustav Fischer Verlag Jena, 1971. Mit 116 Abb. und 237 Tab., Ganzleinen, Format 10,5 × 24,5 cm. Preis: 30,—M. Reviewed by H. Bergner. H. Bergner: Energie — Leben — Leistung — Nahrung. Erschienen im Urania-Verlag Leipzig-Jena-Berlin 1971, 144 Seiten, mit 49 Abb. und 25 Tab., Ganzleinen, Format 14,5 × 21,5 cm, Preis: 9,80 M. Reviewed by H. Haenel. G. Dirksen, W. Kaufmann und E. Pfeffer: Untersuchungen zur Verdauungs physiologie der Milchkuh mit der Umleitungstechnik am Duodenum. Erschienen im Verlag Paul Parey, Hamburg und Berlin 1972. 43 Seiten mit 10 Abb. und 22 Tab., kart., Format 16,5 × 25 cm, Preis: 22,00 DM (als Beiheft zur Z. Tierphys. Tierernähr. Futtermittelkd., für Bezicher der Zeitschrift 20,35 DM). Reviewed by H. Bergner. 相似文献
212.
Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties 总被引:1,自引:0,他引:1
Anja B Dohrmann Meike Küting Sebastian Jünemann Sebastian Jaenicke Andreas Schlüter Christoph C Tebbe 《The ISME journal》2013,7(1):37-49
Ribosomal 16S rRNA gene pyrosequencing was used to explore whether the genetically modified (GM) Bt-maize hybrid MON 89034 × MON 88017, expressing three insecticidal recombinant Cry proteins of Bacillus thuringiensis, would alter the rhizosphere bacterial community. Fine roots of field cultivated Bt-maize and three conventional maize varieties were analyzed together with coarse roots of the Bt-maize. A total of 547 000 sequences were obtained. Library coverage was 100% at the phylum and 99.8% at the genus rank. Although cluster analyses based on relative abundances indicated no differences at higher taxonomic ranks, genera abundances pointed to variety specific differences. Genera-based clustering depended solely on the 49 most dominant genera while the remaining 461 rare genera followed a different selection. A total of 91 genera responded significantly to the different root environments. As a benefit of pyrosequencing, 79 responsive genera were identified that might have been overlooked with conventional cloning sequencing approaches owing to their rareness. There was no indication of bacterial alterations in the rhizosphere of the Bt-maize beyond differences found between conventional varieties. B. thuringiensis-like phylotypes were present at low abundance (0.1% of Bacteria) suggesting possible occurrence of natural Cry proteins in the rhizospheres. Although some genera indicated potential phytopathogenic bacteria in the rhizosphere, their abundances were not significantly different between conventional varieties and Bt-maize. With an unprecedented sensitivity this study indicates that the rhizosphere bacterial community of a GM maize did not respond abnormally to the presence of three insecticidal proteins in the root tissue. 相似文献
213.
S?ren Franzenburg Sebastian Fraune Philipp M Altrock Sven Künzel John F Baines Arne Traulsen Thomas CG Bosch 《The ISME journal》2013,7(4):781-790
Animals are colonized by complex bacterial communities. The processes controlling community membership and influencing the establishment of the microbial ecosystem during development are poorly understood. Here we aimed to explore the assembly of bacterial communities in Hydra with the broader goal of elucidating the general rules that determine the temporal progression of bacterial colonization of animal epithelia. We profiled the microbial communities in polyps at various time points after hatching in four replicates. The composition and temporal patterns of the bacterial communities were strikingly similar in all replicates. Distinct features included high diversity of community profiles in the first week, a remarkable but transient adult-like profile 2 weeks after hatching, followed by progressive emergence of a stable adult-like pattern characterized by low species diversity and the preponderance of the Betaproteobacterium Curvibacter. Intriguingly, this process displayed important parallels to the assembly of human fecal communities after birth. In addition, a mathematical modeling approach was used to uncover the organizational principles of this colonization process, suggesting that both, local environmental or host-derived factor(s) modulating the colonization rate, as well as frequency-dependent interactions of individual bacterial community members are important aspects in the emergence of a stable bacterial community at the end of development. 相似文献
214.
Bacterial species are internally diverse in genomic and multi-locus gene comparisons. The ecological causes of phenotypic and genotypic diversity within species are far less well understood. Here, we focus on the competitive fitness for growth on nutrients within Escherichia coli, an internally rich species. Competition experiments in nutrient-limited chemostats revealed that members of the ECOR collection exhibited a wide continuum of competitive abilities, with some fitter and some less fit than the lab strain MG1655. We observed an inverse relationship between competitiveness and the resistance of strains to detergent and antibiotic, consistent with the notion that membrane permeability and competitive fitness are linked by a trade-off between self-preservation and nutritional competence (SPANC); high permeability has a postulated cost in antibacterial sensitivity whereas a low permeability has a cost in nutrient affinity. Isolates moved along the markedly nonlinear trade-off curve by mutational adaptation; an ECOR strain sensitive to antibacterials and a good competitor was easily converted by mutation into a mutant with higher resistance but poorer competition in the presence of low antibiotic concentrations. Conversely, a resistant ECOR strain changed into a better competitor after a short period of selection under nutrient limitation. In both directions, mutations can affect porin proteins and outer membrane permeability, as indicated by protein analysis, gene sequencing and an independent assay of outer membrane permeability. The extensive, species-wide diversity of E. coli in ecologically important traits can thus be explained as an evolutionary consequence of a SPANC trade-off driven by antagonistic pleiotropy. 相似文献
215.
由致病疫霉Phytophthora infestans引起的晚疫病是马铃薯生产上最严重的病害之一,认识其群体结构特征,可为晚疫病防控策略的制定以及抗病品种的合理布局提供指导。对2009年采自宁夏一个种植有93个品种(品系)的马铃薯种质资源圃的致病疫霉进行了交配型、致病型和线粒体DNA单倍型分析,结果表明,116个致病疫霉菌株中存在A1、A2和自育型3种交配型,发生频率分别为24.1%、57.8%和18.1%,A2交配型为优势类型;对其中43个菌株的致病型进行测试,检测到两种致病类型:1.2.3.4.5.6.7.8.9.10.11和3.4.10,发生频率分别为95.3%和4.7%,可克服所有11个抗病基因的1.2.3.4.5.6.7.8.9.10.11类型占绝对优势;对62个菌株的线粒体DNA单倍型进行分析,检测到Ia和IIa两种类型,发生频率分别为74.2%和25.8%。综合表型和基因型数据分析发现,该马铃薯种质资源圃中致病疫霉群体致病型单一,但致病型毒力因子高度复合;线粒体DNA分析表明,该马铃薯种质资源圃引入了遗传背景较为复杂的致病疫霉"新"群体。 相似文献
216.
【目的】分离并鉴定精噁唑禾草灵高效降解菌株,为开发高效降解菌剂,强化精噁唑禾草灵原位修复,保证黄瓜产品安全提供菌株资源和理论依据。【方法】利用富集培养的方法分离降解菌株,并通过形态学、生理生化特征和16S rRNA基因进化分析进行鉴定;HPLC/MS鉴定菌株降解精噁唑禾草灵的中间产物,采用鸟枪法建库克隆降解过程中关键的水解酶基因,并进行异源表达,利用Michaelis-Menten双倒数曲线图测定酶动力学参数;通过正交试验确定菌株液态发酵参数,并通过对黄瓜灌根接种的方式,研究降解菌株对黄瓜根际土壤中精噁唑禾草灵的降解以及甘露醇对降解效率的强化作用。【结果】Rhodococcus sp. DSB-1在24 h内能将100 mg/L精噁唑禾草灵完全转化为精噁唑禾草灵酸,降解最适温度和pH分别为30℃和8.0。克隆得到一个精噁唑禾草灵水解酶基因,命名为pepE。水解酶PepE对精噁唑禾草灵的K_m为28.2μmol/L,k_(cat)/K_m为11.0 L/(μmol·s)。在发酵温度30℃、通气量1:0.4、搅拌速度200 r/min、培养时间48 h条件下,液态发酵所得菌剂对精噁唑禾草灵的降解效率最高。投加至黄瓜根际的菌株DSB-1可以在黄瓜根系定殖,12d内完全降解黄瓜根际环境中10mg/kg的精噁唑禾草灵。此外还发现添加甘露醇可强化菌株的修复能力,降解效率相对于未添加的处理提高14.8%。【结论】菌株DSB-1具有原位修复精噁唑禾草灵污染土壤的潜力。 相似文献
217.
Plant and soil effects on bacterial communities associated with Miscanthus × giganteus rhizosphere and rhizomes
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Bacterial assemblages, especially diazotroph assemblages residing in the rhizomes and the rhizosphere soil of Miscanthus × giganteus, contribute to plant growth and nitrogen use efficiency. However, the composition of these microbial communities has not been adequately explored nor have the potential ecological drivers for these communities been sufficiently studied. This knowledge is needed for understanding and potentially improving M. × giganteus – microbe interactions, and further enhancing sustainability of M. × giganteus production. In this study, cultivated M. × giganteus from four sites in Illinois, Kentucky, Nebraska, and New Jersey were collected to examine the relative influences of soil conditions and plant compartments on assembly of the M. × giganteus‐associated microbiome. Automated ribosomal intergenic spacer (ARISA) and terminal restriction fragment length polymorphism (T‐RFLP) targeting the nifH gene were applied to examine the total bacterial communities and diazotroph assemblages that reside in the rhizomes and the rhizosphere. Distinct microbial assemblages were detected in the endophytic and rhizosphere compartments. Site soil conditions had strong correlation with both total bacterial and diazotroph assemblages, but in different ways. Nitrogen treatments showed no significant effect on the composition of diazotroph assemblages in most sites. Endophytic compartments of different M. × giganteus plants tended to harbor similar microbial communities across all sites, whereas the rhizosphere soil of different plant tended to harbor diverse microbial assemblages that were distinct among sites. These observations offer insight into better understanding of the associative interactions between M. × giganteus and diazotrophs, and how this relationship is influenced by agronomic and edaphic factors. 相似文献
218.
The pathogenic Wolbachia strain wMelPop rapidly over‐replicates in the brain, muscles, and retina of Drosophila melanogaster, causing severe tissue degeneration and premature death of the host. The unique features of this endosymbiont make it an excellent tool to be used for biological control of insects, pests, and vectors of human diseases. To follow the dynamics of bacterial morphology and titer in the nerve cells we used transmission electron microscopy of 3‐d‐old female brains. The neurons and glial cells from central brain of the fly had different Wolbachia titers ranging from single bacteria to large accumulations, tearing cell apart and invading extracellular space. The neuropile regions of the brain were free of wMelPop. Wolbachia tightly interacted with host cell organelles and underwent several morphological changes in nerve cells. Based on different morphological types of bacteria described we propose for the first time a scheme of wMelPop dynamics within the somatic tissue of the host. 相似文献
219.
Dialeurolonga malleswaramensis Sundararaj (Hemiptera: Aleyrodidae) is a phytophagous sap sucking insect. It infests Polyalthia longifolia, an important avenue tree of India, effective in alleviating noise pollution and having immense medicinal importance. Samples of this insect were collected from Polyalthia longifolia. The cytochrome c oxidase subunit I gene (mtCO1) helped in the molecular characterization of the insect. This study reports the bacterial diversity in D. malleswaramensis adults by high throughput 16S rDNA amplicon pyrosequencing. The major genera identified were Portiera and Arsenophonus. Other bacterial genera detected were uncultured alpha proteobacterium, Sphingopyxis and Methylobacterium. We also employed fluorescence in situ hybridization (FISH) in whole mount samples to confirm the presence of dominant endosymbionts Portiera and Arsenophonus to the bacteriocyte of D. malleswaramensis. This study concludes that combining techniques like 16S rDNA amplicon pyrosequencing and FISH reveal both dominant and rare bacteria. The data also predict the evolutionary position of this pest with respect to other whitefly species using a mitochondrial marker. 相似文献
220.
Local adaptation to temperature in populations and clonal lineages of the Irish potato famine pathogen Phytophthora infestans
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Nicolas Mariette Annabelle Androdias Romain Mabon Roselyne Corbière Bruno Marquer Josselin Montarry Didier Andrivon 《Ecology and evolution》2016,6(17):6320-6331
Environmental factors such as temperature strongly impact microbial communities. In the current context of global warming, it is therefore crucial to understand the effects of these factors on human, animal, or plant pathogens. Here, we used a common‐garden experiment to analyze the thermal responses of three life‐history traits (latent period, lesion growth, spore number) in isolates of the potato late blight pathogen Phytophthora infestans from different climatic zones. We also used a fitness index (FI) aggregating these traits into a single parameter. The experiments revealed patterns of local adaptation to temperature for several traits and for the FI, both between populations and within clonal lineages. Local adaptation to temperature could result from selection for increased survival between epidemics, when isolates are exposed to more extreme climatic conditions than during epidemics. We also showed different thermal responses among two clonal lineages sympatric in western Europe, with lower performances of lineage 13_A2 compared to 6_A1, especially at low temperatures. These data therefore stress the importance of thermal adaptation in a widespread, invasive pathogen, where adaptation is usually considered almost exclusively with respect to host plants. This must now be taken into account to explain, and possibly predict, the global distribution of specific lineages and their epidemic potential. 相似文献