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31.
In vitro vegetative propagation of Chinese cabbage 总被引:1,自引:0,他引:1
Explants from cotyledons, axillary buds, inflorescence stems and flower buds of Brassica campestris ssp. pekinensis (Lour.) Olsson (Chinese cabbage, cv. Wongbok) were cultured on MS medium with growth regulators. Multiple shoots were obtained from cotyledons, axillary buds and flower buds but not from inflorescence stems. Propagation of shoots from cotyledons was more successful than from axillary buds and flower buds. The vegetative propagation rates varied amongst clones derived from cotyledons of the same cultivar and seed lot. The propagation rates of the cotyledon-derived material followed a normal distribution with an average propagation rate of 2.6 shoots per two weeks subculture when cultured on MS media plus 44.4 m benzyladenine (BA) and 14.8 m -indolebutyric acid (IBA). Shoots from three clones were cultured on MS medium with nine different concentrations of BA. The concentration of BA which promoted the highest rate of shoot propagation varied for the three clones and was in the range 44.4 to 177.6 m. 相似文献
32.
N. Hammatt 《World journal of microbiology & biotechnology》1992,8(4):369-377
An increasing world population and rise in demand for tree products, especially wood, has increased the need to produce more timber through planting more forest with improved quality stock. Superior trees are likely to arise from several sources. Firstly, forest trees can be selected from wild populations and cloned using macropropagation techniques already being investigated for fruit tree rootstocks. Alternatively, propagation might be brought aboutin vitro through micropropagation or sustained somatic embryogenesis, with encapsulation of the somatic embryos to form artificial seeds. Tree quality could be improved through increased plant breeding and it is likely that experienced gained, to date, in the breeding of fruit species will be useful in devising strategies for forest trees. Since the development of techniques to regenerate woody plants from explant tissues, cells and protoplasts, it is now feasible to test the use of tissue culture methods to bring about improvements in tree quality. Success has already been achieved for tree species in the generation of somaclonal and protoclonal variation, the formation of haploids, triploids and polyploids, somatic hybrids and cybrids and the introduction of foreign DNA through transformation. This review summarizes the advances made so far in tree biotechnology, and suggests some of the directions that it might take in the future. 相似文献
33.
34.
提出两种功能互相不同的神经细胞组成的复合神经元网络(CNN)模型;导出一种特殊结构的CNN的并行动力学;而且证明了它的稳定性。在这些结果基础上,得到快速的假逆矩阵学习算法。计算机仿真试验证实学习算法与动力学稳定性的正确性,并表现出良好的容错性能与存储容量。 相似文献
35.
Cindy Pfeiffer-Linn Raymon M. Glantz 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1991,168(3):373-381
1. | The actions of GABA on three classes of visual interneurons in crayfish, Procambarus clarkii, medulla externa are examined. The effect of GABA on the visual response is compared to GABA's action on agonist-elicited responses purported to mediate the visual response. |
2. | GABA produces a shunting type of inhibition in medullary amacrine cells which is associated with a small depolarization (Figs. 2, 3), a large increase in input conductance (Gn) and a reversal potential close to rest (Fig. 4). GABA is a potent antagonist to the depolarizing action of acetylcholine (ACh) (Fig. 5). |
3. | GABA depolarizes dimming fibers (Fig. 2), and the response is mediated by an increase in Gn (Fig. 6). GABA antagonizes the light-elicited IPSP and the hyperpolarizing action of ACh (Fig. 7). |
4. | Sustaining fibers (SF) do not appear to have GABA receptors but GABA inhibits the excitatory visual input pathway to the SFs (Fig. 8). Conversely, the GABA antagonist, bicuculline, potentiates the SF light response (Fig. 9). |
5. | GABA has at least three different modes of antagonist action in the medulla: i) Increased conductance and depolarization in dimming fibers and medullary amacrine neurons; ii) Decreased chloride conductance in tangential cells; and iii) An inhibitory action on the visual pathway which drives SFs. |
36.
Callus cultures were established from stem explants of Ruscus hypophyllum on a modified basal medium of Murashige and Skoog (1962) supplemented with 1 mg l-1 2,4-D+0.1 mg l-1 BAP. The optimal 2,4-D concentration for promoting shoot bud formation and growth was 0.05 mg l-1 along with 0.5 mg l-1 BAP. Sixty percent of rootless shoots produced flowers on the regenerating medium. Rooting was induced when shoots were transferred to half strength MS inorganic salts supplemented with 2 mg l-1 IBA. Eighty percent of plants transferred to soil have survived. 相似文献
37.
M Cohn 《Biochimie》1985,67(1):9-27
Two concepts of the evolution and regulation of expression of the combining site repertoire of the immune system, are compared. One view is based on the Associative Recognition Theory as formulated by the author and the other is based on the Idiotype Network Idea as conceived by Jerne. The two concepts are analyzed from the point of view of their logic, internal consistency and factual support. 相似文献
38.
H. Christopher Wilson Nadine C. Milos 《In vitro cellular & developmental biology. Plant》1987,23(5):323-331
Summary This study investigates the nutritional requirements ofXenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells
in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium
is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required
to enhance culture development. These include insulin, α-melanocyte stimulating hormone, somatotropin, luteotrophic hormone,
linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conductive environment tested
for cell migration and adhesion.
This work was supported by establishment and major equipment grants from the Alberta Heritage Foundation for Medical Research
to N. C. M. Nadine C. Milos is a Heritage Medical Research Scholar of the Alberta Heritage Foundation for Medical Research. 相似文献
39.
Masazumi Mitani 《Primates; journal of primatology》1986,27(4):397-412
Japanese monkeys often exchange the particular vocal sound, “coo,” especially when they feed or move as a group. It was considered
that the “coo” sound had no positive social meaning, perhaps because the “coo” sound network and its function were hidden
behind other behavioral observations. For identification of the vocalizer only from hearing the “coo” sound, three phonetic
values, i.e., the “fundamental,” “duration,” and “formants,” plus other characteristics were used as indices of voiceprints. The results indicated that these were effective for identifying
the vocalizer in two-thirds of the adults in the study troop which was composed of 12 adults and 16 immature members. The
“coo” sound exchange network among the troop members (adults) was drawn on the basis of the voiceprint identification. The
network showed three characteristics as follows: (1) matriarchs of the kin-groups frequently exchanged “coo” sounds with each
other; (2) the other females exchanged “coo” sounds mostly within their own kin-groups; and (3) males seldom participated
in the “coo” sound exchange. This suggests that “coo” sound exchange plays a central role for the matriarch of kin-groups
in binding each kin-group and, ultimately, in binding all members together into an organized troop. 相似文献
40.
Michael K. Smith Brenda J. Biggs Kenneth J. Scott 《Plant Cell, Tissue and Organ Culture》1986,6(3):221-228
A method is presented for the rapid in vitro propagation of cassava (Manihot esculenta Crantz). Nodal explants were induced to grow as multiple-shoot cultures on a medium containing 1.0 M 6-benzylamino purine (BAP), supplemented with 0.25 M -naphthaleneacetic acid (NAA). Nodes were removed from the shoots after three weeks of growth and subcultured on fresh culture medium. An average of 7.0 nodes were produced from each explanted node after three weeks in culture. Nodal explants were transferred to a medium containing 2.5 M indole-3-butyric acid (IBA) to improve root initiation on the developing plantlets. Plant establishment was possible upon transfer to soil. In vitro propagation offers enhanced rates of multiplication over more conventional methods of propagation. In addition, in vitro propagation facilitates the storage and international exchange of cassava germplasm. 相似文献