全文获取类型
收费全文 | 666篇 |
免费 | 45篇 |
国内免费 | 12篇 |
出版年
2023年 | 22篇 |
2022年 | 17篇 |
2021年 | 30篇 |
2020年 | 19篇 |
2019年 | 26篇 |
2018年 | 24篇 |
2017年 | 22篇 |
2016年 | 14篇 |
2015年 | 31篇 |
2014年 | 40篇 |
2013年 | 30篇 |
2012年 | 21篇 |
2011年 | 32篇 |
2010年 | 22篇 |
2009年 | 19篇 |
2008年 | 24篇 |
2007年 | 22篇 |
2006年 | 20篇 |
2005年 | 16篇 |
2004年 | 13篇 |
2003年 | 16篇 |
2002年 | 23篇 |
2001年 | 13篇 |
2000年 | 15篇 |
1999年 | 9篇 |
1998年 | 15篇 |
1997年 | 14篇 |
1996年 | 10篇 |
1995年 | 11篇 |
1994年 | 11篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 8篇 |
1982年 | 16篇 |
1981年 | 13篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1976年 | 2篇 |
1973年 | 3篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1970年 | 2篇 |
排序方式: 共有723条查询结果,搜索用时 343 毫秒
71.
Bechara A Nawabi H Moret F Yaron A Weaver E Bozon M Abouzid K Guan JL Tessier-Lavigne M Lemmon V Castellani V 《The EMBO journal》2008,27(11):1549-1562
Axonal receptors for class 3 semaphorins (Sema3s) are heterocomplexes of neuropilins (Nrps) and Plexin-As signalling coreceptors. In the developing cerebral cortex, the Ig superfamily cell adhesion molecule L1 associates with Nrp1. Intriguingly, the genetic removal of L1 blocks axon responses of cortical neurons to Sema3A in vitro despite the expression of Plexin-As in the cortex, suggesting either that L1 substitutes for Plexin-As or that L1 and Plexin-A are both required and mediate distinct roles. We report that association of Nrp1 with L1 but not Plexin-As mediates the recruitment and activation of a Sema3A-induced focal adhesion kinase-mitogen-activated protein kinase cascade. This signalling downstream of L1 is needed for the disassembly of adherent points formed in growth cones and subsequently their collapse response to Sema3A. Plexin-As and L1 are coexpressed and present in common complexes in cortical neurons and both dominant-negative forms of Plexin-A and L1 impair their response to Sema3A. Consistently, Nrp1-expressing cortical projections are defective in mice lacking Plexin-A3, Plexin-A4 or L1. This reveals that specific signalling activities downstream of L1 and Plexin-As cooperate for mediating the axon guidance effects of Sema3A. 相似文献
72.
During development, sensory thalamocortical (TC) axons grow into the neocortex and terminate primarily in layer 4. To study the molecular mechanism that underlies lamina-specific TC axon termination, we investigated the responsiveness of TC axons to ephrin-A5, semaphorin-7A (Sema7A) and kit ligand (KL), which are expressed in the upper layers of the developing cortex. Dissociated cells of the dorsal thalamus from embryonic rat brain were cultured on dishes that were coated with preclustered Fc-tagged extracellular domains of these molecules. Each protein was found to promote TC axon growth in a dose-dependent fashion of a bell-shaped curve. Any combination of the three proteins showed a cooperative effect in lower concentrations but not in higher concentrations, suggesting that their growth-promoting activities act in a common pathway. The effect of spatial distributions of these proteins was further tested on a filter membrane, in which these proteins were printed at a size that recapitulates the scale of laminar thickness in vivo, using a novel protein-printing technique, Simple-To-mAke Micropore Protein-Printing (STAMP2) method. The results demonstrated that TC axons grew massively on the laminin-coated region but were prevented from invading the adjacent ephrin-A5-printed region, suggesting that TC axons detect relative differences in the growth effect between these regions. Moreover, the inhibitory action of ephrin-A5 was enhanced by copresence with KL and Sema7A. Together, these results suggest that the lamina-specific TC axon targeting mechanism involves growth-inhibitory activity by multiple molecules in the upper layers and detection in the molecular environments between the upper and deep layers. 相似文献
73.
Sorting and Transport of Alpha Herpesviruses in Axons 总被引:1,自引:0,他引:1
The alpha herpesviruses, a subfamily of the herpesviruses, are neurotropic pathogens found associated with most mammalian species. The prototypic member of this subfamily is herpes simplex virus type 1, the causative agent of recurrent cold sores in humans. The mild nature of this disease is a testament to the complex and highly regulated life cycle of the alpha herpesviruses. The cellular mechanisms used by these viruses to disseminate infection in the nervous system are beginning to be understood. Here, we overview the life cycle of alpha herpesviruses with an emphasis on assembly and transport of viral particles in neurons. 相似文献
74.
《Developmental cell》2023,58(8):660-676.e7
- Download : Download high-res image (230KB)
- Download : Download full-size image
75.
《Molecular & cellular proteomics : MCP》2022,21(11):100422
Cellular biomolecular complexes including protein–protein, protein–RNA, and protein–DNA interactions regulate and execute most biological functions. In particular in brain, protein–protein interactions (PPIs) mediate or regulate virtually all nerve cell functions, such as neurotransmission, cell–cell communication, neurogenesis, synaptogenesis, and synaptic plasticity. Perturbations of PPIs in specific subsets of neurons and glia are thought to underly a majority of neurobiological disorders. Therefore, understanding biological functions at a cellular level requires a reasonably complete catalog of all physical interactions between proteins. An enzyme-catalyzed method to biotinylate proximal interacting proteins within 10 to 300 nm of each other is being increasingly used to characterize the spatiotemporal features of complex PPIs in brain. Thus, proximity labeling has emerged recently as a powerful tool to identify proteomes in distinct cell types in brain as well as proteomes and PPIs in structures difficult to isolate, such as the synaptic cleft, axonal projections, or astrocyte–neuron junctions. In this review, we summarize recent advances in proximity labeling methods and their application to neurobiology. 相似文献
76.
77.
Ross A. Robinson Samuel C. Griffiths Lieke L. van de Haar Tomas Malinauskas Eljo Y. van Battum Pavol Zelina Rebekka A. Schwab Dimple Karia Lina Malinauskaite Sara Brignani Marleen H. van den Munkhof Özge Düdükcü Anna A. De Ruiter Dianne M.A. Van den Heuvel Benjamin Bishop Jonathan Elegheert A. Radu Aricescu R. Jeroen Pasterkamp Christian Siebold 《Cell》2021,184(8):2103-2120.e31
- Download : Download high-res image (287KB)
- Download : Download full-size image
78.
Synapsins were the first presynaptic proteins identified and have served as the flagship of the presynaptic protein field. Here we review recent studies demonstrating that different members of the synapsin family play different roles at presynaptic terminals employing different types of synaptic vesicles. The structural underpinnings for these functions are just beginning to be understood and should provide a focus for future efforts. 相似文献
79.
Sandra L. Tanner Elaine E. Storm †‡ George D. Bittner 《Journal of neurochemistry》1995,64(4):1491-1501
Abstract: Using video-enhanced microscopy and a pulse-radiolabeling paradigm, we show that proteins synthesized in the medial giant axon cell body of the crayfish ( Procambarus clarkii ) are delivered to the axon via fast (∼62 mm/day) and slow (∼0.8 mm/day) transport components. These data confirm that the medial giant axon cell body provides protein to the axon in a manner similar to that reported for mammalian axons. Unlike mammalian axons, the distal (anucleate) portion of a medial giant axon remains intact and functional for >7 months after severance. This axonal viability persists long after fast transport has ceased and after the slow wave front of radiolabeled protein has reached the terminals. These data are consistent with the hypothesis that another source (i.e., local glial cells) provides a significant amount of protein to supplement that delivered to the medial giant axon by its cell body. 相似文献