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991.
Multiple, superimposed Type I interferon (IFN) restriction fragments were resolved following 72–92 h of horizontal electrophoresis. Restriction fragment length polymorphisms (RFLPs) for α IFN (IFNA), β IFN (IFNB), ωIFN (IFNW) and trophoblast IFN (IFNT) genes were identified in Hin dill, Eco RI and Taql digestions from 313 cattle. RFLPs with codominant segregation in cattle pedigrees were considered alleles, and 19 distinct polymorphic Type I IFN loci (5 IFNA, 4 IFNB, 8 IFNW and 2 IFNT) were identified. Allele frequencies and observed heterozygosity values were calculated for each locus and several loci were considered highly informative for linkage analysis. Bovine IFN gene numbers (10 IFNA, 6 IFNB, 20 IFNW and 6 IFNT) were estimated from the number of polymorphic loci plus additional monomorphic hybridizing bands present in Eco RI and Hindlll digestions. Physical linkage of the Type I IFN gene families on bovine chromosome 8 was demonstrated by pulsed field gel electrophoresis (PFGE). Hybridization of two or more IFN probes to similarly sized PFGE fragments suggested the tentative gene family order: IFNA/IFNW-IFNT-IFNB. These studies provide a basis for the development of more detailed genetic and physical maps of the bovine Type I IFNs. 相似文献
992.
B. Turcq C. Deleu M. Denayrolles J. Bégueret 《Molecular & general genetics : MGG》1991,228(1-2):265-269
Summary Vegetative incompatibility is a lethal reaction that destroys the heterokaryotic cells formed by the fusion of hyphae of non-isogenic strains in many fungi. That incompatibility is genetically determined is well known but the function of the genes triggering this rapid cell death is not. The two allelic incompatibility genes, s and S, of the fungus Podospora anserina were characterized. Both encode 30 kDa polypeptides, which differ by 14 amino acids between the two genes. These two proteins are responsible for the incompatibility reaction that results when cells containing s and S genes fuse. Inactivation of the s or S gene by disruption suppresses incompatibility but does not affect the growth or the sexual cycle of the mutant strains. This suggests that these incompatibility genes have no essential function in the life cycle of the fungus. 相似文献
993.
Gerald H. Jacobs 《International journal of primatology》2007,28(4):729-759
The visual worlds of most primates are rich with potential color signals, and many representatives of the order have evolved
the biological mechanisms that allow them to exploit these sources of information. Unlike the catarrhines, platyrrhines typically
have sex-linked polymorphic color vision that provides individuals with any of several distinct types of color vision, including
both trichromatic and dichromatic variants. In recent years, this polymorphism has been the target of an expanding range of
research efforts. As a result, researchers now reasonably understand the proximate biology underlying the polymorphisms, and
a number of ideas have emerged as to their evolution. Progress has also been made in illuminating how color vision capacities
may be related to the particular visual tasks that New World monkeys face. 相似文献
994.
ADP-ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32P-NAD; then the nucleolar proteins were analyzed by 1-dimensional and 2-dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE-cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP-ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP-ribosylation. 相似文献
995.
996.
S. COTTON 《Journal of evolutionary biology》2009,22(4):899-906
‘Good genes’ models of sexual selection show that females can gain indirect benefits for their offspring if male ornaments are condition‐dependent signals of genetic quality. Recurrent deleterious mutation is viewed as a major contributor to variance in genetic quality, and previous theoretical treatments of ‘good genes’ processes have assumed that the influx of new mutations is constant. I propose that this assumption is too simplistic, and that mutation rates vary in ways that are important for sexual selection. Recent data have shown that individuals in poor condition can have higher mutation rates, and I argue that if both male sexual ornaments and mutation rates are condition‐dependent, then females can use male ornamentation to evaluate their mate’s mutation rate. As most mutations are deleterious, females benefit from choosing well‐ornamented mates, as they are less likely to contribute germline‐derived mutations to offspring. I discuss some of the evolutionary ramifications of condition‐dependent mutation rates and sexual selection. 相似文献
997.
An endogenous virus, denoted ev A, is present at high frequency in all brown egg layer lines. Using inverse polymerase chain reaction (PCR) based on the viral LTR regions, products were obtained containing cellular sequences 5' and 3' to the viral insertion point. PCR of chicken genomic DNA was carried out, using primers chosen from the 5' and 3' cellular sequences and a primer chosen from either the U3 or U5 portions of the viral LTR. Amplification of DNA from birds that did not carry ev A with the primer triplets always gave a single 364bp reaction product, interpreted as representing the flank-to-flank amplification product. Amplification of DNA from known homozygous or heterozygous ev A carriers, with the same primer triplets, always gave both the expected junction product and 364bp product. Therefore, these primer sequences can be used to distinguish ev A carriers from non-carriers but cannot distinguish between homozygous and heterozygous ev A carriers. 相似文献
998.
999.
1000.
N. D. Jambhale Y. S. Nerkar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,71(1):122-125
Summary Genetic studies on radiation-induced chlorina and variegated mutants of okra (Abelmoschus esculentus (L.) Moench) revealed the existence of an unstable gene. The normal green color of the leaves is controlled by duplicate genes C1 and C2, either of which produces the green colour. The chlorina plants are C
1
C
1
C
2
C
2. The allele c
1
v
is dominant to both C
1 and C
2 but is unstable. The homozygote c
1
v
c
1
v
c
2
c
2 is a normal green while the heterozygote c
i
v
c
1
c
2
c
2 has a variegated phenotype as a result of the mutation of c
1
v
to c
1 during development. In green plants with a c
1
v
c{sh1/v}c
2
c
2 genotype, the autonomous mutation of one of the c
1
v
alleles to c
1 may take place at the pre-meiotic stage. In the variegated genotype (c
1
v
c
1
c
2
c
2), the mutation of c
1 to c
1
v
may take place in early ontogeny, thus producing green plants. The allele C
1, when associated with c
1
v
in a heterozygous condition, mutates to c
1 at the pre-meiotic stage even in the presence of the allele C
2. 相似文献