全文获取类型
收费全文 | 634篇 |
免费 | 29篇 |
国内免费 | 9篇 |
出版年
2023年 | 13篇 |
2022年 | 13篇 |
2021年 | 11篇 |
2020年 | 10篇 |
2019年 | 12篇 |
2018年 | 26篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 7篇 |
2014年 | 28篇 |
2013年 | 46篇 |
2012年 | 28篇 |
2011年 | 22篇 |
2010年 | 13篇 |
2009年 | 22篇 |
2008年 | 31篇 |
2007年 | 27篇 |
2006年 | 23篇 |
2005年 | 24篇 |
2004年 | 27篇 |
2003年 | 12篇 |
2002年 | 11篇 |
2001年 | 9篇 |
2000年 | 14篇 |
1999年 | 7篇 |
1998年 | 7篇 |
1997年 | 14篇 |
1996年 | 9篇 |
1995年 | 16篇 |
1994年 | 14篇 |
1993年 | 17篇 |
1992年 | 9篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1989年 | 13篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1985年 | 5篇 |
1984年 | 14篇 |
1983年 | 10篇 |
1982年 | 14篇 |
1981年 | 5篇 |
1980年 | 11篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 6篇 |
1974年 | 6篇 |
1973年 | 5篇 |
排序方式: 共有672条查询结果,搜索用时 281 毫秒
91.
92.
温度对不同类型早籼稻灌浆期间直链淀粉、蛋白质积累的影响 总被引:4,自引:0,他引:4
高温条件下早籼稻籽粒直链淀粉积累速率快,持续时间短;蛋白质含量高;灌浆前期谷草转氨酶和谷丙转氨酶活性高,开花11d后迅速下降.适温条件下籽粒直链淀粉积累速率慢,持续时间长;蛋白质含量低;谷草转氨酶和谷丙转氨酶活性变化缓慢,3个不同类型品种中适合作米粉的湘早籼33号直链淀粉含量最高,饲料稻湘早籼24号蛋白质含量最高.高温有助于湘早籼33号直链淀粉含量和湘早籼24号蛋白质含量的提高。 相似文献
93.
D H Ohlendorf S I Foundling J J Wendoloski J Sedlacek P Strop F R Salemme 《Proteins》1992,14(3):382-391
The structure of the retroviral proteinase from avian myeloblastosis associated virus (MAV) has been determined and refined at 2.2 A resolution. This structure is compared with those of homologous proteinases from Rous sarcoma virus (RSV) and human immunodeficiency type 1 virus (HIV). Through comparison with the structure of a proteinase-inhibitor complex from HIV, a model of a complex between MAV proteinase and a peptide substrate has been generated. Examination of this model suggests structural basis for the diverse specifications of viral proteinases. 相似文献
94.
Although the amino acid glutamate is used as an intercellular signaling molecule for normal bone homeostasis, little is known regarding its possible role in the metabolic disruption characteristic of bone metastasis. We have previously shown in vitro that cancer cell lines relevant to bone metastasis release glutamate into the extracellular environment. This study demonstrates the expression of multiple glutamate transporters in cancer cell lines of non-central nervous system origin. Furthermore, we identify the molecular mechanism responsible for glutamate export and show that this system can be inhibited pharmacologically. By highlighting that glutamate secretion is a common biological feature of cancer cells, this study suggests that tumor-derived glutamate could interfere with glutamate-dependent intercellular signaling in normal bone. Pharmacological interference with cancer cell glutamate release may be a viable option for limiting host bone response to invading tumor cells in bone metastasis. 相似文献
95.
Clinal variation was observed at the aspartate aminotransferase-2 (AAT-2) locus in the marine fish Cynoscion nebulosus (Cuvier) inhabiting the bays and estuaries of the Texas and northern Mexico Gulf coasts. Frequency of the AAT-2(80) allele increased from 0.9% at Sabine Lake, Texas to 17.1% at Rio Soto La Marina, Mexico. A statistically significant correlation existed in the frequency of this allele with degrees north latitude and west longitude. This information, if properly incorporated into a comprehensive enhancement programme, could facilitate supplemental stocking success. 相似文献
96.
Enantioselective biotransformation of sterically hindered amine substrates by the fungus Stemphylium lycopersici
下载免费PDF全文
![点击此处可从《Journal of applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
97.
Joanna Kamińska Olga M. Koper Edyta Siedlecka-Czykier Joanna Matowicka-Karna Jerzy Bychowski Halina Kemona 《Saudi Journal of Biological Sciences》2018,25(7):1263-1271
Introduction
Thrombotic and inflammatory mechanisms are involved in the pathophysiology of acute coronary syndrome (ACS). The aim of the study was the evaluation of inflammation (white blood cells count/WBC, C-reactive protein/CRP, interleukin-6/IL-6) and platelet (platelet count/PLT, mean platelet volume/MPV, large platelet/LPLT, beta-thromboglobulin/β-TG) biomarkers in the groups of ACS patients depending on the severity of signs and symptoms and compared to controls without coronary artery disease.Materials and methods
The study group included 93 patients categorized into 3 subgroups depending on the severity of signs and symptoms of ACS. PLT, MPV, LPLT, and WBC were determined on hematological analyzer, IL-6 and β-TG were measured using the ELISA method.Results
In the whole group of ACS patients WBC, CRP, IL-6, MPV, and β-TG were significantly higher as compared to controls. Analyzing the inflammation and platelet biomarkers depending on the severity of signs and symptoms in comparison to controls, statistically significant differences for above-mentioned parameters were also found. There were no significant differences between the advancement of coronary artery changes and inflammation as well as platelet parameters, except for CRP concentrations. The AUCs for all inflammation parameters tested were similar, however the highest AUCs showed WBC and CRP. Among platelet parameters the highest AUC revealed β-TG.Conclusion
Markers of inflammation and platelet activation may be associated to myocardial ischemia and myocardial injury. WBC, CRP and IL-6 as inflammation parameters and MPV and β-TG as platelet biomarkers may be useful indicators of the presence of coronary artery disease. 相似文献98.
Badr Alzahrani Tristan Iseli Mehdi Ramezani-Moghadam Vikki Ho Miriam Wankell Eun Jin Sun Liang Qiao Jacob George Lionel W. Hebbard 《生物化学与生物物理学报:疾病的分子基础》2018,1864(3):700-708
Activation of the adiponectin (APN) signaling axis retards liver fibrosis. However, understanding of the role of AdipoR1 and AdipoR2 in mediating this response is still rudimentary. Here, we sought to elucidate the APN receptor responsible for limiting liver fibrosis by employing AdipoR1 and AdipoR2 knock-out mice in the carbon tetrachloride (CCl4) model of liver fibrosis. In addition, we knocked down receptor function in primary hepatic stellate cells (HSCs) in vitro. Following the development of fibrosis, AdipoR1 and AdipoR2 KO mice had no quantitative difference in fibrosis by Sirius red staining. However, AdipoR2 KO mice had an enhanced fibrotic signature with increased Col1-α1, TGFß-1, TIMP-1, IL-10, MMP-2 and MMP-9. Knockdown of AdipoR1 or AdipoR2 in HSCs followed by APN treatment demonstrated that AdipoR1 and AdipoR2 did not affect proliferation or TIMP-1 gene expression, while AdipoR2 modulated Col1-α1 and α-SMA gene expression, HSC migration, and AMPK activity. These finding suggest that AdipoR2 is the major APN receptor on HSCs responsible for mediating its anti-fibrotic effects. 相似文献
99.
Dandan Liang Hongjin Chen Leping Zhao Wenxin Zhang Jie Hu Zhiguo Liu Peng Zhong Wei Wang Jingying Wang Guang Liang 《生物化学与生物物理学报:疾病的分子基础》2018,1864(1):133-142
Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease. NAFLD begins with steatosis and advances to nonalcoholic steatohepatitis (NASH) and cirrhosis. The molecular mechanisms involved in NAFLD progression are not understood. Based on recent studies showing dysregulation of epidermal growth factor receptor (EGFR) in animal models of liver injury, we sought to determine if inhibition of EGFR mitigates liver fibrosis and HSC activation in NAFLD. We utilized the high fat diet (HFD)-induced murine model of liver injury to study the role of EGFR in NAFLD. The lipid accumulation, oxidative stress, hepatic stellate cell (HSC) activation and matrix deposition were examined in the liver tissues. We also evaluated the EGFR signaling pathway, ROS activation and pro-fibrogenic phenotype in oxidized low density lipoproteins (ox-LDL) challenged cultured HSCs. We demonstrate that EGFR was phosphorylated in liver tissues of HFD murine model of NAFLD. Inhibition of EGFR prevented diet-induced lipid accumulation, oxidative stress, and HSC activation and matrix deposition. In cultured HSCs, we show that ox-LDL caused rapid activation of the EGFR signaling pathway and induce the production of reactive oxygen species. EGFR also mediated HSC activation and promoted a pro-fibrogenic phenotype. In conclusion, our data demonstrate that EGFR plays an important role in NAFLD and is an attractive target for NAFLD therapy. 相似文献
100.
Verena Hiebl Angela Ladurner Simone Latkolik Verena M. Dirsch 《Biotechnology advances》2018,36(6):1657-1698
Nuclear receptors (NRs) represent attractive targets for the treatment of metabolic syndrome-related diseases. In addition, natural products are an interesting pool of potential ligands since they have been refined under evolutionary pressure to interact with proteins or other biological targets.This review aims to briefly summarize current basic knowledge regarding the liver X (LXR) and farnesoid X receptors (FXR) that form permissive heterodimers with retinoid X receptors (RXR). Natural product-based ligands for these receptors are summarized and the potential of LXR, FXR and RXR as targets in precision medicine is discussed. 相似文献