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81.
Lars-Olof Pålsson Jan P. Dekker Eberhard Schlodder René Monshouwer Rienk van Grondelle 《Photosynthesis research》1996,48(1-2):239-246
Isolated trimeric Photosystem I complexes of the cyanobacterium Synechococcus elongatus have been studied with absorption spectroscopy and site-selective polarized fluorescence spectroscopy at cryogenic temperatures. The 4 K absorption spectrum exhibits a clear and distinct peak at 710 nm and shoulders near 720, 698 and 692 nm apart from the strong absorption profile located at 680 nm. Deconvoluting the 4 K absorption spectrum with Gaussian components revealed that Synechococcus elongatus contains two types of long-wavelength pigments peaking at 708 nm and 719 nm, which we denoted C-708 and C-719, respectively. An estimate of the oscillator strengths revealed that Synechococcus elongatus contains about 4–5 C-708 pigments and 5–6 C-719 pigments. At 4 K and for excitation wavelengths shorter than 712 nm, the emission maximum appeared at 731 nm. For excitation wavelengths longer than 712 nm, the emission maximum shifted to the red, and for excitation in the far red edge of the absorption spectrum the emission maximum was observed 10–11 nm to the red with respect to the excitation wavelength, which indicates that the Stokes shift of C-719 is 10–11 nm. The fluorescence anisotropy, as calculated in the emission maximum, reached a maximal anisotropy of r=0.35 for excitation in the far red edge of the absorption spectrum (at and above 730 nm), and showed a complicated behavior for excitation at shorter wavelengths. The results suggest efficient energy transfer routes between C-708 and C-719 pigments and also among the C-719 pigments.Abbreviations Chl
chlorophyll
- FWHM
full width at half maximum
- PS I
Photosystem I 相似文献
82.
Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes 总被引:1,自引:0,他引:1
The role of plant antioxidant system in water stress tolerance was studied in three contrasting wheat genotypes. Water stress
imposed at different stages after anthesis resulted in a general increase in lipid peroxidation (LPO) and decrease in membrane
stability index (MSI), and contents of chlorophylls (Chl) and carotenoids (Car). Antioxidant enzymes like glutathione reductase
and ascorbate peroxidase significantly increased under water stress. Genotype C 306, which had highest glutathione reductase
and ascorbate peroxidase activity, also showed lowest LPO and highest MSI, and Chl and Car contents under water stress in
comparison to susceptible genotype HD 2329, which showed lowest antioxidant enzyme activity as well as MSI, Chl and Car contents
and highest LPO. HD 2285 which is tolerant to high temperature during grain filling period showed intermediate behaviour.
Thus, the relative tolerance of a genotype to water stress as reflected by its comparatively lower LPO and higher MSI, Chl
and Car contents is closely associated with its antioxidant enzyme system.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
83.
Isolated antennae of the male moth Antheraea polyphemus adsorbed at least 32% of 3H-labelled pheromone molecules (E-6,Z-11 hexadecadienyl acetate) from an airstream passing the antenna. About 80% of the adsorbed molecules were caught by the long olfactory hairs (sensilla trichodea). The distal half of the hairs caught about twice as many molecules as the proximal half. About 40% of the molecules desorbed if the antennae were exposed to a clean airstream for 30 min. The adsorbed molecules were transported from the hairs towards the antennal branch. Transport due to diffusion would have a diffusion coefficient of 3 × 10?7 cm2/s. Forty per cent of the total radioactivity per hair could be detected in receptor lymph extruded from the olfactory hairs, after an incubation time of 2 min. Dried antennae showed an increased desorption and an increased velocity of the transport along the hairs. One interpretation is that the molecules enter the receptor lymph of the intact antennae and diffuse more slowly than those on the cuticular surface. Fractional elution of fresh antennae revealed a diminishing elutability of pheromone from antennae in pentane (DEP-effect) and almost constant elutability in more polar solvents (chloroform-methanol toluene). The DEP-effect could be reversibly abolished by dehydration of the antennae. It could be shown that the DEP-effect occurs mainly on the antennal branch rather than on the hairs. Residual (uneluted) radioactivity builds up mainly on the branch. 相似文献
84.
An O2-barrier in the intact light-harvesting complex LHC II protects chlorophylls (Chl) and xanthophylls (Car) from photooxidation. Direct evidence for the limited access of O2 to pigment sites comes from the decay kinetics of the first excited triplet state of Car (3Car-). The LHC-bound 3Car- in air-saturated solution decays mono-exponentially with a lifetime of 6.7-7.1 µs as compared to the approx. 1.2 µs of the -carotene triplet in hexane and the 8.8-9 µs observed for both systems under anaerobiosis. Further properties of the photostable complex are the limited access of protons to pigment sites and the efficient energy transfer from 1Car- to Chl-a and from 3Chl- to Car. Fatty acids with increasing chain length increasingly lower both, the efficiency of the O2 barrier and the photo- and acid stability of the LHC-bound pigments while singlet and triplet energy transfer between the pigments is maintained. Therefore, the close proximity of Chl and Car is not sufficient to protect the pigments from photooxidation; in addition, an O2-barrier limiting the access of O2 to pigment sites is required for efficient photoprotection. Structural properties of the photostable LHC II possibly underlying its O2-barrier function are discussed. 相似文献
85.
Alan F. Burke 《ZooKeys》2013,(299):49-75
Six new Cymatodera speciesfrom the Mexican states of Jalisco and Chiapas, and the Central American countries of El Salvador, Honduras, Costa Rica and Panamá are described: Cymatodera rosalinae
sp. n., Cymatodera capax
sp. n., Cymatodera sinuosa
sp. n., Cymatodera vittata
sp. n., Cymatodera rubida
sp. n. and Cymatodera limatula
sp. n. Justification for retaining Cymatodera obliquefasciata within Cymatodera instead of transferring it to Bogcia is provided. Male genitalia and other characters of taxonomic value are illustrated. 相似文献
86.
Terje Bjørnland 《Phytochemistry》1982,21(7):1715-1719
Monoalgal cultured Eutreptielia gymnastica contained chlorophyll a and b. The acetylenic carotenoids diatoxanthin and diadinoxanthin were among the main xanthophylls while their non-acetylenic analogues zeaxanthin and antheraxanthin were absent. The structurally most complex carotenoid was identical with neoxanthin. Three of the xanthophylls isolated could not be positively correlated with carotenoids previously reported from the Euglenophyceae. The ketocarotenoids astaxanthin, canthaxanthin and echinenone were absent. 相似文献
87.
The antennal morphology and chaetotaxy were studied in 52 species of the endogean carabid genus Typhlocharis, using scanning electron microscopy and light microscopy. The antennae are composed of 11 antennomeres (scape, pedicel, and nine flagellomeres). We found considerable variation between species in the third antennomere, with short‐stem and long‐stem forms, and flagellomere morphology, distinguishing two morphs: rounded (subovoid, subspheric and subquadrate, morph 1) and reniform shapes (morph 2). Antennal sensilla are grouped in six types of sensilla trichodea, three types of sensilla basiconica, one type of sensilla coeloconica, and one type of sensilla campaniformia. The distribution of sensilla along the antennomeres is described. The “rings” of trichoid sensilla in the antennomere body are affected by its shape and there is interspecific variation in the pattern of sensilla coeloconica in antennomere 11°, a novelty for the genus. The types of sensilla found in Typhlocharis are compared to those described in other Carabidae and the potential functionality and taxonomic interest of those variable antennal features are discussed. A correlation between the flagellomere morphology and the presence/absence of a stridulatory organ is suggested. The study also allowed comparing the observation of antennal features by SEM and light microscopy. J. Morphol., 2013. © 2013 Wiley Periodicals, Inc. 相似文献
88.
Sissel Norgård W.A. Svec Synnöve Liaaen-Jensen A. Jensen R.R.L. Guillard 《Biochemical Systematics and Ecology》1974,2(1):3-6
The chloroplast pigments of one typical representative (Pleurochloris magna) and two potential members (clone BSG Sticho and an isolate called Tunis) of the new class Eustigmatophyceae have been examined by modern methods including mass spectrometry. The three cultures all exhibited the same chloroplast pigments: Chlorophyll a, but no b or c, δ-carotene (I), canthaxanthin (II), violaxanthin (IIIa), and esterified vaucheriaxanthin (IVb) plus some free vaucheriaxanthin (IVa). Furanoid rearrangement of the epoxidic carotenoids complicated the analysis. The unique pigment complement hereby indicated for Eustigmatophyceae is clearly different from the pigment distribution patterns reported for Chlorophyceae and Xanthophyceae. 相似文献
89.
Magnetic material in the body parts of the stingless bee Schwarziana quadripunctata, heads, pairs of antennae, thorax and abdomens, were investigated by SQUID magnetometry and Ferromagnetic Resonance (FMR).
The saturation, Js and remanent, Jr, magnetizations and coercive field Hc are determined from the hysteresis curves. From Hc and Jr/Js the magnetic particle sizes are estimated. The Js and the FMR spectral absorption areas yield 23±3%, 45±5%, 15±2% and 19±4% magnetic material contributions of head, pair of
antennae, thorax and abdomen, respectively, similar to those observed in the migratory ant Pachycondyla marginata. This result is discussed in light of the hypothesis of antennae as a magnetosensor structure. 相似文献
90.