全文获取类型
收费全文 | 802篇 |
免费 | 56篇 |
国内免费 | 27篇 |
出版年
2024年 | 3篇 |
2023年 | 12篇 |
2022年 | 20篇 |
2021年 | 33篇 |
2020年 | 22篇 |
2019年 | 29篇 |
2018年 | 21篇 |
2017年 | 26篇 |
2016年 | 34篇 |
2015年 | 28篇 |
2014年 | 45篇 |
2013年 | 50篇 |
2012年 | 19篇 |
2011年 | 31篇 |
2010年 | 26篇 |
2009年 | 38篇 |
2008年 | 44篇 |
2007年 | 50篇 |
2006年 | 31篇 |
2005年 | 38篇 |
2004年 | 28篇 |
2003年 | 36篇 |
2002年 | 22篇 |
2001年 | 22篇 |
2000年 | 19篇 |
1999年 | 17篇 |
1998年 | 13篇 |
1997年 | 11篇 |
1996年 | 19篇 |
1995年 | 7篇 |
1994年 | 17篇 |
1993年 | 14篇 |
1992年 | 13篇 |
1991年 | 3篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有885条查询结果,搜索用时 27 毫秒
41.
J. Poissant M. B. Morrissey A. G. Gosler J. Slate B. C. Sheldon 《Journal of evolutionary biology》2016,29(10):2022-2035
When selection differs between the sexes for traits that are genetically correlated between the sexes, there is potential for the effect of selection in one sex to be altered by indirect selection in the other sex, a situation commonly referred to as intralocus sexual conflict (ISC). While potentially common, ISC has rarely been studied in wild populations. Here, we studied ISC over a set of morphological traits (wing length, tarsus length, bill depth and bill length) in a wild population of great tits (Parus major) from Wytham Woods, UK. Specifically, we quantified the microevolutionary impacts of ISC by combining intra‐ and intersex additive genetic (co)variances and sex‐specific selection estimates in a multivariate framework. Large genetic correlations between homologous male and female traits combined with evidence for sex‐specific multivariate survival selection suggested that ISC could play an appreciable role in the evolution of this population. Together, multivariate sex‐specific selection and additive genetic (co)variance for the traits considered accounted for additive genetic variance in fitness that was uncorrelated between the sexes (cross‐sex genetic correlation = ?0.003, 95% CI = ?0.83, 0.83). Gender load, defined as the reduction in a population's rate of adaptation due to sex‐specific effects, was estimated at 50% (95% CI = 13%, 86%). This study provides novel insights into the evolution of sexual dimorphism in wild populations and illustrates how quantitative genetics and selection analyses can be combined in a multivariate framework to quantify the microevolutionary impacts of ISC. 相似文献
42.
43.
The main goal of the present study was to characterise the course of infection and immunological responses developed by Leishmania infantum infected BALB/c mice. Parasite load was determined by Real-time TaqMan PCR while cytokine and Immunoglobulin G (IgG) production were assessed by ELISA. Leishmania DNA was detected in spleen and liver as soon as day 1 post-inoculation (pi) and the parasitism was sustained until the end of the experiment. The cytokine kinetics in spleen and liver was generally associated with the oscillations of parasite load. Overall, it was not observed a distinct Th1 or Th2 pattern of cytokine production during the time of experiment. The infected mice developed a mixed immune response, with concomitant production of IFN-gamma, IL-4 and IL-10, both in spleen and liver, and both IgG isotypes. However, our results suggest that, compared to liver, the spleen is more susceptible to L. infantum infection. 相似文献
44.
The comparability and reliability of global positioning system (GPS) devices during running protocols associated with team-sports was investigated. Fourteen moderately-trained males completed 690 m of straight-line movements, a 570 m change of direction (COD) course and a 642.5 m team-sport simulated circuit (TSSC); on two occasions. Participants wore a FieldWiz GPS device and a Catapult MinimaxX S4 10-Hz GPS device. Typical error of measurement (TE) and coefficient of variation (CV%) were calculated between GPS devices, for the variables of total distance and peak speed. Reliability comparisons were made within FieldWiz GPS devices, between sessions. Small TE were observed between FieldWiz and Catapult GPS devices for total distance and peak speed during straight-line (16.9 m [2%], 1.2 km·h−1 [4%]), COD (31.8 m [6%], 0.4 km·h−1 [2%]) and TSSC protocols (12.9 m [2%], 0.5 km·h−1 [2%]), respectively, with no significant mean bias (p > 0.05). Small TE were also observed for the FieldWiz GPS device between sessions (p > 0.05) for straight-line (9.6 m [1%], 0.2 km·h−1 [1%]), COD (12.8 m [2%], 0.2 km·h−1 [1%]) and TSSC protocols (6.9 m [1%], 0.6 km·h−1 [2%]), respectively. Data from the FieldWiz GPS device appears comparable to established devices and reliable across a range of movement patterns associated with team-sports. 相似文献
45.
The human cervical spine supports substantial compressive load in vivo. However, the traditional in vitro testing methods rarely include compressive loads, especially in investigations of multi-segment cervical spine constructs. Previously, a systematic comparison was performed between the standard pure moment with no compressive loading and published compressive loading techniques (follower load – FL, axial load – AL, and combined load – CL). The systematic comparison was structured a priori using a statistical design of experiments and the desirability function approach, which was chosen based on the goal of determining the optimal compressive loading parameters necessary to mimic the segmental contribution patterns exhibited in vivo. The optimized set of compressive loading parameters resulted in in vitro segmental rotations that were within one standard deviation and 10% of average percent error of the in vivo mean throughout the entire motion path. As hypothesized, the values for the optimized independent variables of FL and AL varied dynamically throughout the motion path. FL was not necessary at the extremes of the flexion–extension (FE) motion path but peaked through the neutral position, whereas, a large negative value of AL was necessary in extension and increased linearly to a large positive value in flexion. Although further validation is required, the long-term goal is to develop a “physiologic” in vitro testing method, which will be valuable for evaluating adjacent segment effect following spinal fusion surgery, disc arthroplasty instrumentation testing and design, as well as mechanobiology experiments where correct kinematics and arthrokinematics are critical. 相似文献
46.
The Static Optimization (SO) solver in OpenSim estimates muscle activations and forces that only equilibrate applied moments. In this study, SO was enhanced through an open-access MATLAB interface, where calculated muscle activations can additionally satisfy crucial mechanical stability requirements. This Stability-Constrained SO (SCSO) is applicable to many OpenSim models and can potentially produce more biofidelic results than SO alone, especially when antagonistic muscle co-contraction is required to stabilize body joints. This hypothesis was tested using existing models and experimental data in the literature. Muscle activations were calculated by SO and SCSO for a spine model during two series of static trials (i.e. simulation 1 and 2), and also for a lower limb model (supplementary material 2). In simulation 1, symmetric and asymmetric flexion postures were compared, while in simulation 2, various external load heights were compared, where increases in load height did not change the external lumbar flexion moment, but necessitated higher EMG activations. During the tasks in simulation 1, the predicted muscle activations by SCSO demonstrated less average deviation from the EMG data (6.8% −7.5%) compared to those from SO (10.2%). In simulation 2, SO predicts constant muscle activations and forces, while SCSO predicts increases in the average activations of back and abdominal muscles that better match experimental data. Although the SCSO results are sensitive to some parameters (e.g. musculotendon stiffness), when considering the strategy of the central nervous system in distributing muscle forces and in activating antagonistic muscles, the assigned activations by SCSO are more biofidelic than SO. 相似文献
47.
Yan‐Hui Zhao Amparo Lzaro Zong‐Xin Ren Wei Zhou Hai‐Dong Li Zhi‐Bin Tao Kun Xu Zhi‐Kun Wu Lorne M. Wolfe De‐Zhu Li Hong Wang 《Oikos》2019,128(4):551-562
Pollination networks are usually constructed and assessed by direct field observations which commonly assume that all flower visitors are true pollinators. However, this assumption is often invalid and the use of data based on mere visitors to flowers may lead to a misunderstanding of intrinsic pollination networks. Here, using a large dataset by both sampling floral visitors and analyzing their pollen loads, we constructed 32 networks pairs (visitation versus pollen transport) across one flowering season at four elevation sites in the Himalaya–Hengduan Mountains region. Pollen analysis was conducted to determine which flower visitors acted as potential pollinators (pollen vectors) or as cheaters (those not carrying pollen of the visited plants). We tested whether there were topological differences between visitation and pollen transport networks and whether different taxonomic groups of insect visitors differed in their ability to carry pollen of the visited plants. Our results indicated that there was a significantly higher degree of specialization at both the network and species levels in the pollen transport networks in contrast to the visitation networks. Modularity was lower but nestedness was higher in the visitation networks compared to the pollen transport networks. All the cheaters were identified as peripheral species and most of them contributed positively to the nested structure. This may explain in part the differences in modularity and nestedness between the two network types. Bees carried the highest proportion of pollen of the visited plants. This was followed by Coleoptera, other Hymenoptera and Diptera. Lepidoptera carried the lowest proportion of pollen of the visited plants. Our study shows that the construction of pollen transport networks could provide a more in‐depth understanding of plant–pollinator interactions. Moreover, it suggests that detecting and removing cheater interactions when studying the topology of other mutualistic networks might be also important. 相似文献
48.
Jakub Marynowicz Mateusz Lango Damian Horna Karol Kikut Marcin Andrzejewski 《Biology of sport / Institute of Sport》2022,39(2):245
The purpose of this study was to determine the effectiveness of white-box decision tree models (DTM) for predicting the rating of perceived exertion (RPE). The second aim was to examine the relationship between RPE and external measures of intensity in youth soccer training at the group and individual level. Training load data from 18 youth soccer players were collected during an in-season competition period. A total of 804 training observations were undertaken, with a total of 43 ± 17 sessions per player (range 12–76). External measures of intensity were determined using a 10 Hz GPS and included total distance (TD, m/min), high-speed running distance (HSR, m/min), PlayerLoad (PL, n/min), impacts (n/min), distance in acceleration/deceleration (TD ACC/TD DEC, m/min) and the number of accelerations/decelerations (ACC/DEC, n/min). Data were analysed with decision tree models. Global and individualized models were constructed. Aggregated importance revealed HSR as the strongest predictor of RPE with relative importance of 0.61. HSR was the most important factor in predicting RPE for half of the players. The prediction error (root mean square error [RMSE] 0.755 ± 0.014) for the individualized models was lower compared to the population model (RMSE 1.621 ± 0.001). The findings demonstrate that individual models should be used for the assessment of players’ response to external load. Furthermore, the study demonstrates that DTM provide straightforward interpretation, with the possibility of visualization. This method can be used to prescribe daily training loads on the basis of predicted, desired player responses (exertion). 相似文献
49.
Shuyu Liu Lei Zhang Yupeng Sang Qiang Lai Xinxin Zhang Changfu Jia Zhiqin Long Jiali Wu Tao Ma Kangshan Mao Nathaniel R Street Pr K Ingvarsson Jianquan Liu Jing Wang 《Molecular biology and evolution》2022,39(2)
Hybridization and resulting introgression are important processes shaping the tree of life and appear to be far more common than previously thought. However, how the genome evolution was shaped by various genetic and evolutionary forces after hybridization remains unresolved. Here we used whole-genome resequencing data of 227 individuals from multiple widespread Populus species to characterize their contemporary patterns of hybridization and to quantify genomic signatures of past introgression. We observe a high frequency of contemporary hybridization and confirm that multiple previously ambiguous species are in fact F1 hybrids. Seven species were identified, which experienced different demographic histories that resulted in strikingly varied efficacy of selection and burdens of deleterious mutations. Frequent past introgression has been found to be a pervasive feature throughout the speciation of these Populus species. The retained introgressed regions, more generally, tend to contain reduced genetic load and to be located in regions of high recombination. We also find that in pairs of species with substantial differences in effective population size, introgressed regions are inferred to have undergone selective sweeps at greater than expected frequencies in the species with lower effective population size, suggesting that introgression likely have higher potential to provide beneficial variation for species with small populations. Our results, therefore, illustrate that demography and recombination have interplayed with both positive and negative selection in determining the genomic evolution after hybridization. 相似文献
50.
P. Loth A. Herschel K. Schrter H.-P. Liebert 《International Review of Hydrobiology》1995,80(4):535-561
The effects of a decreased sewage release from a cellulose mill on the hypertrophic Bleiloch reservoir were studied. The waste water treatment reduced the concentration of yellow substances in the reservoir and resulted in a more light-coloured waterbody. It is estimated that the changes in the light attenuation in the Bleiloch reservoir after 1989 can be described as eutrophication. Selected physical, chemical, and biological parameters from 1994 show the effect of changes. Three large pelagic enclosures (diameter 10 m, length 20 m) were installed in the reservoir to assess effects of artifical mixing on phytoplankton biomass and composition. Two circulation techniques have been used in the enclosures (relase of compressed air at the bottom of the enclosure and mechanical mixing, where water was pumped from the surface downward). The investigation demonstrate that the magnitude of seasonal peaks of chlorophyll were depressed in the mixed enclosures during circulation. The results were discussed in comparison with a reference enclosure without mixing and the waterbody of the reservoir. Cryptophytes and other flagellates achieved dominance during mixing in the “air”-enclosure, while diatoms were the most abundant species in the „pump”︁-enclosure. The development of filamentous cyanobacteria, which are significant indicators of eutrophication, was disturbed in the enclosures. They were outcompeted by cryptophytes, movable chlorophytes and diatoms. Both mechanical pump and compressed air can be used to control excessive algal growth. Further studies on the dynamics of phytoplankton to artifical mixing are suggested. The results of further studies should design an in situ experiment in the reservoir to reduce algal growth by light limitation. Further on a dense duckweed cover spreads over the surface of the Bleiloch reservoir, which influences the light regime in the waterbody. Therefore factors influencing the duckweed growth were analysed. Special attention was focused on the influence of the pH, consequences of the mixing in the waterbody and the role of resting stages. 相似文献