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161.
162.
Calystegines in wild and cultivated Erythroxylum species 总被引:1,自引:0,他引:1
Calystegines were identified in the genus Erythroxylum for the first time. Erythroxylum novogranatense var. novogranatense, a species cultivated for cocaine production, contained 0.2% total calystegines in dry leaves. Forty six Erythroxylum herbarium species consisting mostly of leaf tissue were analysed for calystegines, and 38 were found positive. Calystegines were compared qualitatively and quantitatively between individual Erythroxylum species. Calystegines A(3) and B(2) were the major calystegines in most species. Total calystegine content reached up to 0.32% dry mass. The simultaneous occurrence of calystegines, cocaine, other alkaloids of a 3alpha-hydroxy- or 3beta-hydroxytropane structure together with nicotine supports the concept of common biosynthetic steps of these alkaloids in Erythroxylum. The present results are the basis for further investigations of the phylogenetic origin of tropane alkaloid biosynthesis in the taxonomically remote families Solanaceae and Erythroxylaceae. 相似文献
163.
Acetylajmalan esterase (AAE) plays an essential role in the late stage of ajmaline biosynthesis. Based on the partial peptide
sequences of AAE isolated and purified from Rauvolfia cell suspensions, a full-length AAE cDNA clone was isolated. The amino acid sequence of AAE has the highest level of identity
of 40% to putative lipases known from the Arabidopsis thaliana genome project. Based on the primary structure AAE is a new member of the GDSL lipase superfamily. The expression in Escherichia coli failed although a wide range of conditions were tested. With a novel virus-based plant expression system, it was possible
to express AAE functionally in leaves of Nicotiana benthamiana Domin. An extraordinarily high enzyme activity was detected in the Nicotiana tissue, which exceeded that in Rauvolfia serpentina (L.) Benth. ex Kurz cell suspension cultures about 20-fold. This expression allowed molecular analysis of AAE for the first
time and increased the number of functionally expressed alkaloid genes from Rauvolfia now to eight, and the number of ajmaline pathway-specific cDNAs to a total of six.
The nucleotide sequence reported in this article has been submitted to the GenBank/EBI under GenBank Accession no. AY762990 相似文献
164.
Although comparatively little research has been undertaken into the secondary metabolites of bryozoans as compared with those of other marine invertebrates, bryozoans have proven to be an excellent source of novel and/or biologically active compounds. The majority of bryozoan metabolites isolated to date have been alkaloids. In our continuing search for bioactive and/or novel compounds from New Zealand marine bryozoans, we undertook an investigation of an extract of Pterocella vesiculosa (order Cheilostomatida, suborder Ascophorina, family Catenicellidae) which possessed activity against P388 murine leukaemia cells. Two alkaloids, pterocellins A–B (1–2) have been isolated from the bryozoan. The biological activity of these alkaloids was examined including their activities in the in vitro 60 cell line panel and in vivo hollow fibre assays at the National Cancer Institute (NCI). The isolation and characterisation of further pterocellin analogues is currently in progress and tentative structures for two new members of this series, pterocellins C–D (3–4) are proposed, based on NMR and mass spectral data. 相似文献
165.
Panzer A Joubert AM Bianchi PC Hamel E Seegers JC 《European journal of cell biology》2001,80(1):111-118
Chelidonine is a tertiary benzophenanthridine alkaloid known to cause mitotic arrest and to interact weakly with tubulin. Our interest in chelidonine began when we found it to be a major contaminant of Ukrain, which is a compound reported to be selectively toxic to malignant cells. The effects of chelidonine in two normal (monkey kidney and Hs27), two transformed (Vero and Graham 293) and two malignant (WHCO5 and HeLa) cell lines, were examined. Chelidonine proved to be a weak inhibitor of cell growth, but no evidence for selective cytotoxicity was found in this study. It was confirmed that chelidonine inhibits tubulin polymerisation (IC50 = 24 microM), explaining its ability to disrupt microtubular structure in cells. A G2/M arrest results, which is characterised by abnormal metaphase morphology, increased levels of cyclin B1 and enhanced cdc2 kinase activity. Exposure of all cell lines examined to chelidonine leads to activation of the stress-activated protein kinase/jun kinase pathway (SAPK/JNK). 相似文献
166.
膝瓣乌头中生物碱成分的研究 总被引:2,自引:0,他引:2
从膝瓣乌头(Aconitum geniculatum)根中共分得14单体成分,我谱法分别鉴定为,黄草乌碱甲、丙(vilmorrianines,A,C)1和2、乌碱(yunnaconitine)3、南乌碱乙(AustroconitineB)4、印乌碱(indaconitine)5,8-乙酰-14-苯甲酰尼奥宁(8-acetyl-14-enzoyineoline)8、塔拉萨敏(talatizminie 相似文献
167.
苦豆子生物碱的研究与苦豆子综合利用 总被引:15,自引:0,他引:15
本文综述槐属植物苦豆子的生物学特性,自然资源,综合利用及其生物碱的研究。 相似文献
168.
169.
170.
The chemical investigation of Pseudoceratina sp. led to the isolation of six compounds including tyrosine brominated isoxazoline alkaloids (1 and 2), polyketide (3), and 5α,8α-epidioxysterols (4–6). The structures of these compounds were identified by comparison of NMR and MS spectroscopic data with those previously reported. Compounds 3–6 were isolated for the first time from sponges of the genus Pseudoceratina. The chemotaxonomic significance of these compounds was discussed. 相似文献