全文获取类型
收费全文 | 1171篇 |
免费 | 50篇 |
国内免费 | 65篇 |
出版年
2024年 | 4篇 |
2023年 | 18篇 |
2022年 | 9篇 |
2021年 | 11篇 |
2020年 | 22篇 |
2019年 | 46篇 |
2018年 | 20篇 |
2017年 | 41篇 |
2016年 | 32篇 |
2015年 | 30篇 |
2014年 | 48篇 |
2013年 | 74篇 |
2012年 | 29篇 |
2011年 | 38篇 |
2010年 | 24篇 |
2009年 | 43篇 |
2008年 | 56篇 |
2007年 | 49篇 |
2006年 | 47篇 |
2005年 | 38篇 |
2004年 | 37篇 |
2003年 | 29篇 |
2002年 | 30篇 |
2001年 | 31篇 |
2000年 | 27篇 |
1999年 | 34篇 |
1998年 | 19篇 |
1997年 | 17篇 |
1996年 | 23篇 |
1995年 | 21篇 |
1994年 | 30篇 |
1993年 | 22篇 |
1992年 | 22篇 |
1991年 | 18篇 |
1990年 | 25篇 |
1989年 | 23篇 |
1988年 | 8篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 17篇 |
1984年 | 27篇 |
1983年 | 6篇 |
1982年 | 20篇 |
1981年 | 17篇 |
1980年 | 12篇 |
1979年 | 16篇 |
1978年 | 11篇 |
1977年 | 8篇 |
1976年 | 18篇 |
1975年 | 4篇 |
排序方式: 共有1286条查询结果,搜索用时 31 毫秒
171.
Algal nutrient enrichment bioassays were conducted between May 1975 and August 1978 using water samples collected from Chautauqua Lake, New York. Photosynthetic fixation rates of natural phytoplankton assemblages were enhanced by additions of phosphorus and nitrogen, although enrichment with other nutrients had no significant stimulatory effect on algal photosynthesis. Whereas phosphorus stimulated in spring and early summer, both nitrogen and phosphorus enhanced photosynthesis in midsummer and fall. Relative to the effect of phosphorus enrichment, enhancement of photosynthesis by nitrogen during the summer and fall was highest in the northern part of the lake. During the period of ice cover, photosynthesis did not appear to be limited by nutrients in that nutrient additions (P, N, Si, C, Fe, trace metals) did not enhance fixation rates. Observed temporal fluctuations in the response of the algae to P and N correlated with changes in the lake water N:P ratio as well as with temporal changes in dissolved orthophosphate and nitrate-nitrite nitrogen. The N:P ratio decreased drastically in the summer and remained at ca. 10 or less through mid-fall, suggesting that N concentrations were inadequate for the non-N-fixing phytoplankton. Studies over 3 yr indicate that states of P and N limitation undergo time-space fluctuations that occur in a cyclic pattern in the surface waters of Chautauqua Lake. 相似文献
172.
Naval J. Antia Joseph Y. Cheng Richard A. J. Foyle Elizabeth Percival 《Journal of phycology》1979,15(1):57-62
The extracellular starch released by autolysis of glycerol-grown Chroomonas salina (Wislouch) Butcher was purified and chemically characterized. It contained 99%D-glucose, formed a blue complex (λmax at 605 nm) with iodine, and showed an optical rotation value more positive than that of potato starch. Methylation analysis established the presence of 1,4- and 1,4,6-glucoside linkages in the proportion 15:1. These properties indicate the cryptomonad starch to be an iodophilic α-(1,4)-glucan, composed of ca. 30% amylose with amylopectin, and broadly resembling potato, Chilomonas and other unicellular algal starches. 相似文献
173.
The abundance of calcareous green algae was recorded quarterly at 28 sites within the Florida Keys National Marine Sanctuary (FKNMS) for a period of 7 years as part of a sea grass monitoring program. To evaluate the validity of using the functional‐form group approach, we designed a sampling method that included the functional‐form group and the component genera. This strategy enabled us to analyze the spatiotemporal patterns in the abundance of calcareous green algae as a group and to describe synchronous behavior among its genera through the application of a nonlinear regression model to both categories of data. Spatial analyses revealed that, in general, all genera displayed long‐term trends of increasing abundance at most sites; however, at some sites the long‐term trends for genera opposed one another. Strong synchrony in the timing of seasonal changes was found among all genera, possibly reflecting similar reproductive and seasonal growth pattern, but the variability in the magnitude of seasonal changes was very high among genera and sites. No spatial patterns were found in long‐term or seasonal changes; the only significant relation detected was for slope, with sites closer to land showing higher values, suggesting that some factors associated with land proximity are affecting this increase. We conclude that the abundances of genera behaved differently from the functional‐form group, indicating that the use of the functional‐form group approach may be unsuitable to detect changes in sea grass community structure in the FKNMS at the existing temporal and spatial scale of the monitoring program. 相似文献
174.
A common garden experiment with Porphyra umbilicalis (Rhodophyta) evaluates methods to study spatial differences in the macroalgal microbiome
下载免费PDF全文
![点击此处可从《Journal of phycology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Charlotte T. C. Quigley Hilary G. Morrison Inara R. Mendonça Susan H. Brawley 《Journal of phycology》2018,54(5):653-664
While macroalgal microbiomes are the focus of many recent studies, there is little information about microbial spatial diversity across the thallus. Reliance on field material makes it difficult to discern whether recovered microbiomes belong to the host or its epiphytes, and technical comparisons of macroalgal samples for microbial studies are needed. Here, we use a common garden approach that avoids the problem of epiphytes, particularly at holdfasts, to examine the microbiome of Porphyra umbilicalis (strain Pum1). We used the V6 hypervariable region of the 16S rDNA with Illumina HiSeq sequencing and developed PNA clamps to block recovery of organelle V6 sequences. The common garden approach allowed us to determine differences in the microbiome at the holdfast versus blade margin. We found a notable increase in the relative abundance of Planctomycetes and Alphaproteobacteria at the holdfast, particularly of the possible symbiont Sulfitobacter sp. Nonadjacent 1.5 cm2 samples of blade margin had microbiomes that were not statistically different. The most abundant phylum in the overall microbiome was Proteobacteria, followed by Bacteroidetes. Because phycologists often work in remote sites, we compared three stabilization and preparation techniques and found silica gel desiccation/bead‐beating and flash‐freezing/lyophilization/bead‐beating to be interchangeable. Core taxa (≥0.1% of sequences) across treatments were similar and accounted for ≥95% of all sequences. Finally, statistical conclusions for all comparisons were the same, regardless of which microbial community analysis tool was used: mothur or minimum entropy decomposition. 相似文献
175.
Circumtropical distribution of the epiphytic dinoflagellate Coolia malayensis (Dinophyceae): Morphology and molecular phylogeny from Puerto Rico and Brazil
下载免费PDF全文
![点击此处可从《Phycological Research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Fernando Gómez Dajun Qiu Ernesto Otero‐Morales Rubens M Lopes Senjie Lin 《Phycological Research》2016,64(3):194-199
The dinoflagellate genus Coolia, which contains potentially toxic species, is an important component of epiphytic assemblages in marine ecosystems. The morphology of C. malayensis has been illustrated from strains isolated in Asia and Oceania. In this study, strains of C. malayensis isolated from the Caribbean Sea in Puerto Rico, and for the first time from the South Atlantic Ocean in Brazil, were investigated by light, epifluorescence and scanning electron microscopies. No significant morphological differences between these new strains and other geographically distant strains of C. malayensis were observed. In the LSU rDNA phylogeny, the C. malayensis sequences from Brazil and Puerto Rico branched within the clade of strains from Oceania and Asia. The recently described species C. santacroce branched as a sister group of C. monotis, and C. palmyrensis was basal to the combined group of C. monotis/C. malayensis/C. santacroce. A tentative undescribed species from Florida and New Zealand branched as a sister group of C. malayensis. Our results confirm that C. malayensis showed a cosmopolitan distribution in tropical to subtropical waters, while the type species C. monotis remains endemic for the Mediterranean Sea and the temperate North Atlantic. 相似文献
176.
Oxytetracycline age validation of an adult shortfin mako shark Isurus oxyrinchus after 6 years at liberty
下载免费PDF全文
![点击此处可从《Journal of fish biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
This study presents findings on an oxytetracycline injected adult male shortfin mako Isurus oxyrinchus recaptured in waters off of southern California after 6 years at liberty. During the period at liberty, the vertebral band‐pair deposition rate was validated at one per year. This result indicates that from a time at or near sexual maturity, male I. oxyrinchus in the north‐east Pacific Ocean exhibit a band‐pair deposition rate of one band pair per year, while deposition rates for juveniles in the area have been validated at two band pairs per year. 相似文献
177.
An earlier reported method for revealing latent periodicity of the nucleotide sequences has been considerably modified in a case of small samples, by applying a Monte Carlo method. This improved method has been used to search for the latent periodicity of some nucleotide sequences of the EMBL data bank. The existence of the nucleotide sequences' latent periodicity has been shown for some genes. The results obtained have implied that periodicity of gene structure is projected onto the periodicity of primary amino acid sequences and, further, onto spatial protein conformation. Even though the periodic structure of gene sequences has been eroded, it is still retained in primary and/or spatial structures of corresponding proteins. Furthermore, in a few cases the study of genes' periodicity has suggested their possible evolutionary origin by multifold duplications of some gene's fragments. 相似文献
178.
Photosynthetic inorganic carbon utilization and growth of Porphyra linearis (Rhodophyta) 总被引:2,自引:0,他引:2
Alvaro Israel Shlomit Katz Zvy Dubinsky John E. Merrill Michael Friedlander 《Journal of applied phycology》1999,11(5):447-453
Photosynthetic (oxygen evolution) and growth (biomass increase) responses to ambient pH and inorganic carbon (Ci) supply were
determined for Porphyralinearis grown in 0.5 L glass cylinders in the laboratory, or in 40 L fibreglass outdoor tanks with running seawater. While net photosynthetic
rates were uniform at pH 6.0–8.0, dropping only at pH 8.7, growth rates were significantly affected by pH levels other than
that of seawater (c. pH 8.3). In glass cylinders, weekly growth rates averaged 76% at external pH 8.0, 13% at pH 8.7 and 26%
at pH 7.0. Photosynthetic O2 evolution on a daily basis(i.e. total O2 evolved during day time less total O2 consumed during night time) was similar to the growth responses at all experimental pH levels, apparently due to high dark
respiration rates measured at acidic pH. Weekly growth rates averaged 53% in algae grown in fibreglass tanks aerated with
regular air (360 mg L-1 CO2) and 28% in algae grown in tanks aerated with CO2-enriched air (750 mg L-1 CO2). The pH of the seawater medium in which P. linear is was grown increased slightly during the day and only rarely reached 9.0. The pH at the boundary layer of algae submerged
in seawater increased in response to light reaching, about pH 8.9 within minutes, or remained unchanged for algae submerged
in a CO2-free artificial sea water medium. Photosynthesis of P. linearissaturated at Ci concentrations of seawater (K0.5560 μM at pH 8.2) and showed low photosynthetic affinity for CO2(K0.5 61 μM) at pH 6.0. It is therefore concluded that P. linearisuses primarily CO2 with HCO3
- being an alternative source of Ci for photosynthesis. Its fast growth could be related to the enzyme carbonic anhydrase whose
activity was detected intra- and extracellularly.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
179.
Diel feeding rhythms and daily food consumption of juvenile Atlantic salmon in the River Alta, northern Norway 总被引:2,自引:0,他引:2
P.-A. Amundsen ¶ R. Bergersen † H. Huru ‡ T. G. Heggberget § 《Journal of fish biology》1999,54(1):58-71
Atlantic salmon fry (0+) sampled from the River Alta exhibited only minor differences in stomach content weights and feeding rates throughout diel periods, but feeding rates were generally lowest at night. In contrast, salmon parr (1+ to 3+) had large diel fluctuations in stomach content weight, with the largest weights usually being recorded during the night and early morning. Accordingly, their feeding rates were highest at night. This nocturnal feeding pattern was consistent throughout all sampling occasions, and appeared to reflect a persistent feeding periodicity in the salmon parr. The daily food consumption rates of both fry and parr were highest during midsummer and decreased towards autumn. 相似文献
180.
Computer analyses of various genome sequences revealed the existence of certain periodical patterns of adenine–adenine dinucleotides
(ApA). For each genome sequence of 13 eubacteria, 3 archaebacteria, 10 eukaryotes, 60 mitochondria, and 9 chloroplasts, we
counted frequencies of ApA dinucleotides at each downstream position within 50 bp from every ApA. We found that the complete
genomes of all three archaebacteria have clear ApA periodicities of about 10 bps. On the other hand, all of the 13 eubacteria
we analyzed were found to have an ApA periodicity of about 11 bp. Similar periodicities exist in the 10 eukaryotes, although
higher organisms such as primates tend to have weaker periodic patterns. None of the mitochondria and chroloplasts we analyzed
showed an evident periodic pattern.
Received: 3 November 1998 / Accepted: 24 March 1999 相似文献