云南短头熊蜂的繁育特性研究

短头熊蜂Bombus breviceps是云南省优势蜂种,为更好地挖掘本土熊蜂资源,繁育农业授粉蜂群,本研究从云南3个地区（屏边、个旧和昆明）收集短头熊蜂蜂王后,在相同条件下饲养,统计分析其蜂王产卵前期、工蜂发育期、蜂群生长特性、群势、产卵蜂王率及蜂群可应用率等繁育特性指标。结果表明：3个地区工蜂的发育期相同,屏边地区的蜂王产卵前期最长,蜂群始见工蜂时间及工蜂数量达到6头、30头和60头的时间也最长,均显著高于个旧地区和昆明地区（P<0.05）。3个地区短头熊蜂的群势差异不显著（P>0.05）,但昆明地区的工蜂数量最少,显著低于屏边地区和个旧地区（P<0.05）,而子代蜂王数量显著高于屏边地区和个旧地区（P<0.05）,雄性蜂数量显著高于屏边地区（P<0.05）。昆明地区的产卵蜂王率和蜂群可应用率高于屏边地区和个旧地区。总体而言,云南的短头熊蜂群势强,产卵蜂王率75%以上,蜂群可应用率均在63%以上,易于人工繁育,具有重要的授粉利用价值。     

Protein enrichment and digestibility of soft rush (<Emphasis Type="Italic">Juncus effusus</Emphasis>) and rice residues using edible mushrooms <Emphasis Type="Italic">Pleurotus ostreatus</Emphasis> and <Emphasis Type="Italic">Pleurotus sajor-caju</Emphasis>

Pleurotus species are found to be among the most efficient lignocellulolytic types of white-rot fungi. Rice is the main grain cultivated in the extreme south of Brazil. Defatted rice bran and straw are by-products of low aggregate value. Soft rush (Juncus effusus) is a common native plant also very abundant in the region. In the present work, we evaluated changes in substrate composition after growth of two white-rot fungal species: Pleurotus ostreatus and Pleurotus sajor-caju, aiming to increase protein content and digestibility from substrates through solid fermentations and obtain edible mushrooms of high aggregate value. For that, defatted rice bran, defatted rice straw and soft rush were utilized as substrate. The influence of the variables thermal treatment temperature of substrate, substrate moisture and concentration were evaluated on the protein content, digestibility and biological efficiency. The highest protein enrichment of rice bran in P. sajor-caju-fermented medium was due the fact that there was no fructification in these media, while for the P. ostreatus-fermented medium, part of the synthesized protein was converted into mushrooms. The highest protein enrichments were verified in medium with 80% moisture and 25% soft rush (47.1% using P. ostreatus and 49.0% using P. sajor-caju). A higher digestible protein increase was obtained for both species in media with 70% moisture and 25% soft rush.     

武汉市农地非市场价值评估

估算农地资源的非市场价值,将其纳入资源成本核算体系,不仅能够弥补市场机制作用不足给农地城市流转决策带来的影响,而且通过提高土地资源农业利用的比较效益,能够起到缓解农地流失的作用。运用条件价值评估法对武汉市不同类型农地的非市场价值进行估算,在对471份有效问卷进行统计分析后的研究表明：（1）随着农地保护意识的增强,武汉居民已经意识到农地资源非市场价值的存在,83．65％的受访居民对农地保护有正的支付意愿;（2）从居民参与农地保护的支付意愿（Willingness to pay,WTP）出发,全市居民每年保护农地的支付意愿总价值为30．0035×10^8元,折合单位hm^2农地的非市场价值为116154元;（3）从农民作为农地保护执行主体参与农地保护、接受政府补偿（willingness to accept,WTA）的角度出发,农地非市场价值16447．57元／hm^2,是目前国家鼓励农民种植粮食作物发放补贴（中稻225元／（hm^2·a^-1）,折合无限年期价值4978元／hm^2）的3．30倍;（4）从城市居民作为农地保护的间接受益者,假设城市周边农地被城市建设用地征收后带来环境损失,城市居民为接受征地计划、家庭每年愿意接受政府补偿（willingness to accept,WTA）的角度出发,平均每户家庭年均需要接受政府补偿8612元,按当前城市居民户数计算,全市城市居民每年对农地损失的受偿接受金额为109．5685×10^8元,折合单位hm^2农地非市场价值42．42×10^4元。     

基于多源遥感数据的农业干旱监测模型构建及应用

干旱监测问题是干旱灾害模拟与预警及旱灾防灾减灾的关键。基于2001-2013年淮河流域40个气象站资料、28个土壤墒情站点和中分辨率成像光谱仪（MODIS）多源遥感数据,采用SEN趋势法和标准化降水蒸散指数（SPEI）等方法,综合了大气-植被-土壤相互作用等多元成因,构建了适用于淮河流域多源综合遥感干旱监测模型,探讨淮河流域干旱时空规律。研究表明：（1）基于多源数据构建综合干旱监测模型,利用土壤墒情和典型年份干旱监测对综合干旱监测模型适用性进行评价,通过了P < 0.01的显著性检验,构建的模型可综合反映出农业和气象干旱多源信息;（2）淮河流域干旱面积和干旱频率大都集中在4-5月和7-9月,9月份受旱面积最大。河南省是淮河流域受旱频率最高,其干旱面积占淮河流域多年平均干旱面积比重最大（38%）,其次是安徽（22%）,旱地受旱面积比重大于水田受旱面积比重;（3）淮河流域2、3和5月干旱有显著减弱趋势;而1、4和6月则有增强趋势。淮河流域小麦灌浆-成熟时期（4-6月）缺水对小麦粮食产量影响显著,综合淮河流域干旱趋势变化,需强化淮河流域4月份小麦的干旱监测与旱灾预警。     

Nickel in soil and maize plants grown on an oxisol treated over a long time with sewage sludge

Abstract

The major limitation for the use of sewage sludge in agriculture is the risk of soil contamination with heavy metals, and their possible transference to man via the food chain. The objective of this study was to evaluate the content of nickel (Ni) in soil by the two methods of digestion (HNO₃ + H₂O₂ + HCl and HClO₄ + HF), and in different parts of maize plants grown on a tropical soil classified as Typic Eutrorthox, that had been treated with sewage sludge for nine consecutive years, and the effects on dry matter and grain production. The experiment was carried out under field conditions in Jaboticabal-SP, using a randomized block design with four treatments and five replicates. Treatments consisted of: 0.0 (control, mineral fertilization), 45.0, 90.0 and 127.5t ha^?1 sewage sludge (dry basis), accumulated during nine years. Sewage sludge was manually applied to the soil and incorporated at 0.1 m depth before sowing the maize. Soil Ni evaluated by Jackson’s method was 76.8% higher than evaluated by the United States Environmental Protection Agency method that digests the samples by heating with concentrated HNO³, H²O² and HCl. Sewage sludge rates did not affect Ni content in the soil. Ni was accumulated in leaf and stem but was not detected in grain. Sewage sludge and mineral fertilization applied to soil for a long time caused similar effects on dry matter and grain production.     

Electron transfer in a human inducible nitric oxide synthase oxygenase/FMN construct co-expressed with the N-terminal globular domain of calmodulin

The FMN-heme intraprotein electron transfer (IET) kinetics in a human inducible NOS (iNOS) oxygenase/FMN (oxyFMN) construct co-expressed with NCaM, a truncated calmodulin (CaM) construct that includes only its N-terminal globular domain consisting of residues 1-75, were determined by laser flash photolysis. The IET rate constant is significantly decreased by nearly fourfold (compared to the iNOS oxyFMN co-expressed with full length CaM). This supports an important role of full length CaM in proper interdomain FMN/heme alignment in iNOS. The IET process was not observed with added excess EDTA, suggesting that Ca²⁺ depletion results in the FMN domain moving away from the heme domain. The results indicate that a Ca²⁺-dependent reorganization of the truncated CaM construct could cause a major modification of the NCaM/iNOS association resulting in a loss of the IET.     

农田景观格局变化对昆虫的生态学效应

景观格局变化是全球变化的一个重要内容。农田是由人类赖以生存所种植的人工栽培作物组成的生态系统。在该景观系统中,多种植物-害虫-天敌相互作用、相互制约,形成有机整体。研究农田景观格局对害虫和天敌种群动态影响,不仅在害虫生物防治的实践中有重要意义,而且对于揭示人类活动对生物多样性结构与功能的影响,阐明农田景观中生物多样性整合、维持机理有重大的理论意义。本文从农田景观格局的"质、量、形、度"4个方面,系统地论述了农田景观格局变化对害虫和天敌的作用,分析了农田景观的生物控制服务功能,指出了未来研究发展的方向。     

黄土区农业景观空间格局分析

景观空间格局分析是景观生态学研究的核心问题之一。本文用地理信息系统,分维分析和统计分析相结合,以１∶１万土地利用现状图为基础,选取斑块大小、分维数、斑块伸长指数、多样性、优势度、相对丰富度、破碎度等指标,陕北米脂县泉家沟流域农业景观的空间格局。结果显示,乔木林地、坡耕地、果园、草地和梯田农地的分维数较低,坝地、水库、灌溉农田、居信用地和灌木林地的人维数较高,斑块形状较为复杂。随着斑块面积的增加,农