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101.
Photoaccumulations in light trap experiments have been studied in the desmids, Cosmarium, Micrasterias and Euastrum. Dependence of accumulation density on exposure time follows saturation curves, while dose response curves show optima. Time-lapse microcinematography and population methods have revealed that all three basic light-induced motor responses known in microorganisms participate in producing photoaccumulations in desmids. During the initial phase the cells are phototactically attracted towards the trap by scattered light. In low light intensity traps photokinetic reactions may play only a minor role, since photokinesis could be evoked only by light intensities100 lx in Cosmarium cucumis. True photophobic reactions have been demonstrated for the first time in desmids. There are two types of phobic responses in desmids: either the cell reverses its movement or it swings sidewise into the new direction. Behaviour of partially shadowed cells suggests that perception of light direction is brough about by simultaneous intensity measurement at two or more sites within the cell. 相似文献
102.
T Raposa 《Mutation research》1978,57(2):241-251
The effect of various cytostatic drugs was studied on the frequency of sister chromatid exchanges (SCEs) in vitro and in PHA-stimulated lymphocytes of leukaemic patients under cytostatic therapy. The lymphocyte system is a sensitive one for the detection of DNA damage after administration of cytostatic drugs in vitro. Mitomycin C, busulphan, vincristine, chlorambucil, cytosine arabinoside, cyclophosphamide and lycurim were tested. All except cyclophosphamide induced high frequencies of SCEs in the first mitosis after their administration. The experiments with PHA-stimulated lymphocytes in vivo from patients treated with cytostatics showed that cytosine arabinoside, in combination with thioguanine, did not induce higher frequencies of SCEs, whereas in patients who were treated with cyclophosphamide alone or in combination with other cytostatic drugs, there was a higher incidence of SCEs during treatment. About 10 days after the termination of the treatment the elevated freuqencies of SCEs returned to the initial level. After administration of some mutagens, especially alkylating agents in vivo, the lymphocyte system can be used to assess induced DNA repair by continuously monitoring for SCEs. 相似文献
103.
MIXED MODEL APPROACHES FOR ESTIMATING GENETIC VARIANCES AND COVARIANCES 总被引:62,自引:4,他引:58
The limitations of methods for analysis of variance(ANOVA)in estimating genetic variances are discussed. Among the three methods(maximum likelihood ML, restricted maximum likelihood REML, and minimum norm quadratic unbiased estimation MINQUE)for mixed linear models, MINQUE method is presented with formulae for estimating variance components and covariances components and for predicting genetic effects. Several genetic models, which cannot be appropriately analyzed by ANOVA methods, are introduced in forms of mixed linear models. Genetic models with independent random effects can be analyzed by MINQUE(1)method whieh is a MINQUE method with all prior values setting 1. MINQUE(1)method can give unbiased estimation for variance components and covariance components, and linear unbiased prediction (LUP) for genetic effects. There are more complicate genetic models for plant seeds which involve correlated random effects. MINQUE(0/1)method, which is a MINQUE method with all prior covariances setting 0 and all prior variances setting 1, is suitable for estimating variance and covariance components in these models. Mixed model approaches have advantage over ANOVA methods for the capacity of analyzing unbalanced data and complicated models. Some problems about estimation and hypothesis test by MINQUE method are discussed. 相似文献
104.
畜禽遗传资源冷冻保存中的取样方法探讨 总被引:1,自引:0,他引:1
本文基于遗传学和数理统计原理,提出了运用冷冻生殖细胞的方法长期保存畜禽遗传资源时,冷冻生殖细胞的4种取样方法,并推证了其误差公式和最低保存数量的估计公式。最后通过实例对公式的实际应用作了说明和讨论分析。 相似文献
105.
106.
E. C. Holmes 《Journal of molecular evolution》1991,33(3):209-215
Summary In an attempt to resolve some points of branching order in the phylogeny of the eutherian mammals, a phylogenetic analysis of 26 nuclear and 6 mitochondrial genes was undertaken using a maximum likelihood method on a constant rate stochastic model of molecular evolution. Seventeen of the nuclear genes gave a primates/artiodactyls grouping highest support whereas three of the mitochondrial genes found a rodents/artiodactyls grouping to be best supported. The primates/rodents grouping was never the best supported. On the assumption that rodents are indeed an outgroup to primates and artiodactyls and that the latter taxa diverged 70 million years ago, an estimation was made, for each gene, of the time of divergence of the rodent lineage. In most cases such estimates were beyond the limits set by present interpretations of the paleontological record as were many estimates of the divergence time of mouse and rat. These results suggest that, although there is locus variation, the divergent position of the rodent lineage may be an artifact of an elevated rate of nucleotide substitution in this order. 相似文献
107.
李凤民;苗云;张振万 《武汉植物学研究》1991,9(1):75-80
本文提出了一种植物生态学数学模型参数估计的新方法——线性规划法,并结合最小二乘法对模型参数的估计进行了实例分析。认为某种程度上前者更优于后者。是一种值得进一步研究的方法。 相似文献
108.
本文报道用自旋非限制Hartree-Fock方法(spin unrestricted Hartree-Fock method简写UHF)对氧合血红蛋白的Fe-O2键合态进行ab initio研究。结果表明Fe(Ⅱ)、Oc和Or的Mulliken布居值分别为24.18、8.19和7.64。这说明氧合血红蛋白的Fe-O2键合态不发生电子转移。研究模型所得到的频率与实验频率基本一致。研究结果不支持Weiss提出的Fe3+O2-模型,为解释血红蛋白传输氧的作用机理提供了新的理论依据。 相似文献
109.
110.
Dinesh D. Vaishnav Eric T. Korthals 《Journal of industrial microbiology & biotechnology》1991,8(3):209-212
Summary Degradation of 10 organic chemicals by pre-acclimated microorganisms in BOD dilution water was determined directly by UV spectrophotometry and indirectly by a modified BOD method. Residual chemical concentrations were periodically measured and pseudo-first-order biodegradation rate constants (k
1) were calculated. Thek
1 spectrophotometry values ranged from 0.006/h to 0.077/h andk
1-BOD values from 0.002/h to 0.043/h for 1-methylnaphthalene and indole, respectively. The ratios ofk spectrophotometry to k1-BOD were between 1.5 for salicylic acid and 3.0 for 1-methylnaphthalene with a mean of 2.7. A significant (=0.001) linear correlation (r
2=0.854,F=46.630) existed between the two sets of rate constants. Results from this study suggest that the modified BOD method may be used to estimate chemical biodegradation rates in synthetic media. 相似文献