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111.
McCue JM Driscoll WJ Mueller GP 《Biochemical and biophysical research communications》2008,365(2):322-327
Long chain fatty acyl glycines are an emerging class of biologically active molecules that occur naturally and produce a wide array of physiological effects. Their biosynthetic pathway, however, remains unknown. Here we report that cytochrome c catalyzes the synthesis of N-arachidonoyl glycine (NAGly) from arachidonoyl coenzyme A and glycine in the presence of hydrogen peroxide. The identity of the NAGly product was verified by isotope labeling and mass analysis. Other heme-containing proteins, hemoglobin and myoglobin, were considerably less effective in generating arachidonoyl glycine as compared to cytochrome c. The reaction catalyzed by cytochrome c in vitro points to its potential role in the formation of NAGly and other long chain fatty acyl glycines in vivo. 相似文献
112.
Toshifumi Miyazawa Tomoyuki Yukawa Takashi Koshiba Shinichi Ueji Ryoji Yanagihara Takashi Yamada 《Biotechnology letters》2001,23(19):1547-1550
2-(Substituted phenoxy)-1-propanols, e.g. 2-(4-chlorophenoxy)-1-propanol, belonging to primary alcohols with an oxygen atom at the stereocenter, were resolved with moderate to good enantioselectivity, as judged by the value of enantiomeric ratio E (up to 27), through the enantioselective acylation with vinyl butanoate mediated by the little-known lipase from Achromobacter sp. in diisopropyl ether, after the examination of potential factors affecting the reaction such as organic solvents and acyl donors. 相似文献
113.
Ulrich Schulte 《Journal of bioenergetics and biomembranes》2001,33(3):205-212
Proteins specifically involved in the biogenesis of respiratory complex I in eukaryotes have been characterized. The complex I intermediate associated proteins CIA30 and CIA84 are tightly bound to an assembly intermediate of the membrane arm. Like chaperones, they are involved in multiple rounds of membrane arm assembly without being part of the mature structure. Two biosynthetic subunits of eukaryotic complex I have been characterized. The acyl carrier subunit is needed for proper assembly of the peripheral arm as well as the membrane arm of complex I. It may interact with enzymes of a mitochondrial fatty acid synthetase. The 39/40-kDa subunit appears to be an isomerase with a tightly bound NADPH. It is related to a protein family of reductases/isomerases. Both subunits have been discussed to be involved in the synthesis of a postulated, novel, high-potential redox group. 相似文献
114.
Genetic approaches for controlling ratios of related polyketide products in fermentation processes 总被引:2,自引:0,他引:2
A Cropp S Chen H Liu W Zhang K A Reynolds 《Journal of industrial microbiology & biotechnology》2001,27(6):368-377
Simple acyl thioesters are used as precursors for both the initiation and elongation steps in polyketide biosynthetic processes.
Several structurally related polyketide products are sometimes made in these processes. These analogs are typically generated
by a combination of two factors: availability of structurally similar biosynthetic precursors, and biosynthetic enzymes unable
to effectively discriminate between them. Often, only one polyketide product is desired from a fermentation process, requiring
a method to control the ratio of these different analogs. Preferential production of one desired analog is accomplished using
random mutagenesis and manipulation of fermentation conditions. A genetic enzymatic understanding of polyketide biosynthesis,
as well as the pathways that provide the relevant precursors, allows for a rational and more contemporary approach for control
of analogs produced in fermentation processes. This approach involves genetic manipulation of either the pathways that provide
pools of the acyl CoA thioester precursors, or the function/specificity of the appropriate biosynthetic enzymes. Reviewed
herein are three such examples where these approaches have been carried out successfully with polyketide biosynthetic processes.
Journal of Industrial Microbiology & Biotechnology (2001) 27, 368–377.
Received 01 March 2001/ Accepted in revised form 08 August 2001 相似文献
115.
Effect of acyl donor chain length and sugar/acyl donor molar ratio on enzymatic synthesis of fatty acid fructose esters 总被引:3,自引:0,他引:3
Samia Soultani Jean-Marc Engasser Mohamed Ghoul 《Journal of Molecular Catalysis .B, Enzymatic》2001,11(4-6):725-731
Lipase-catalyzed synthesis of fatty acid sugar esters through direct esterification was performed in 2-methyl 2-butanol as solvent. Fructose and saturated fatty acids were used as substrates and the reaction was catalyzed by immobilized Candida antarctica lipase. The effect of the initial fructose/acyl donor molar ratio and the carbon-chain length of the acyl donor as well as their reciprocal interactions on the reaction performance were investigated. For this purpose, an experimental design taking into account variations of the molar ratio (from 1:1 to 1:5) and the carbon-chain length of the fatty acid (from C8 to C18) was employed. Statistical analysis of the data indicated that the two factors as well as their interactions had significant effects on the sugar esters synthesis. The obtained results showed that whatever the molar ratio used, the highest concentration (73 g l−1), fructose and fatty acid conversion yields (100% and 80%, respectively) and initial reaction rate (40 g l−1 h−1) were reached when using the C18 fatty acid as acyl donor. Low molar ratios gave the best fatty acid conversion yields and initial reaction rates, whereas the best total sugar ester concentrations and fructose conversion yields were obtained for high molar ratios. 相似文献
116.
《Molecular cell》2020,77(1):120-137.e9
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117.
In the enantioselective hydrolysis of the di-O-acetyl derivatives of meso-1,3-diol catalyzed by lipases, racemization of the monoacetate products occurs due to non-enzymatic general base-catalyzed acyl migration. The rate of acyl migration increases with increase of pH and buffer concentration. A mechanism of the migration has been proposed to proceed through a six-member ring transition that accounts for the experimental results. The acyl migration, however, was not observed in the enantioselective transesterification of meso-1,3-diols in neutral organic solvents. 相似文献
118.
A survey of a range of plant tissues showed that the hydroxycinnamate CoA ligase in crude extracts of pea shoots had a high relative activity towards sinapic and other methoxycinnamic acids, together with high activity with p-coumaric acid. The pea enzyme has been resolved by chromatography on DEAE-cellulose into two peaks which differ in their substrate specificity. The form which elutes at relatively low salt concentrations has a ratio activity towards p-coumaric and sinapic acids of about 1.8:1 while the form eluting at higher salt concentrations, although showing very high activity with p-coumaric acid, is inactive towards sinapic acid. The pattern of elution of these forms following gel filtration on Ultragel AcA 34 and Sephadex G100 suggests that these two isoenzymes which differ in ionic properties and substrate specificity can exist in two or three molecular weight forms and there is evidence that these forms are under certain circumstances interconvertible. 相似文献
119.
120.