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41.
The acetyl-CoA pathway of autotrophic growth 总被引:3,自引:0,他引:3
Abstract The most direct conceivable route for synthesis of multicarbon compounds from CO2 is to join two molecules of CO2 together to make a 2-carbon compound and then polymerize the 2-carbon compound or add CO2 successively to the 2-carbon compound to make multicarbon compounds. Recently, it has been demonstrated that the bacterium, Clostridium thermoaceticum , grows autotrophically by such a process. The mechanism involves the reduction of one molecule of CO2 to a methyl group and then its combination with a second molecule of CO2 and CoA to form acetyl-CoA. We have designated this autotrophic pathway the acetyl-CoA pathway [1]. Evidence is accumulating that this pathway is utilized by other bacteria that grow with CO2 and H2 as the source of carbon and energy. This group includes bacteria which, like C. thermoaceticum , produce acetate as a major end product and are called acetogens or acetogenic bacteria. It also includes the methane-producing bacteria and sulfate-reducing bacteria.
The purpose of this review is to examine critically the evidence that the acetyl-CoA pathway occurs in other bacteria by a mechanism that is the same or similar to that found in C. thermoaceticum . For this purpose, the mechanism of the acetyl-CoA pathway, as found in C. thermoaceticum , is described and hypothetical mechanisms for other organisms are presented based on the acetyl-CoA pathway of C. thermoaceticum . The available data have been reviewed to determine if the hypothetical schemes are in accord with presently known facts. We conclude that the formation of acetyl-CoA by other acetogens, the methanogens and sulphate-reducing bacteria occurs by a mechanism very similar to that of C. thermoaceticum . 相似文献
The purpose of this review is to examine critically the evidence that the acetyl-CoA pathway occurs in other bacteria by a mechanism that is the same or similar to that found in C. thermoaceticum . For this purpose, the mechanism of the acetyl-CoA pathway, as found in C. thermoaceticum , is described and hypothetical mechanisms for other organisms are presented based on the acetyl-CoA pathway of C. thermoaceticum . The available data have been reviewed to determine if the hypothetical schemes are in accord with presently known facts. We conclude that the formation of acetyl-CoA by other acetogens, the methanogens and sulphate-reducing bacteria occurs by a mechanism very similar to that of C. thermoaceticum . 相似文献
42.
Baltimore Harbor (Baltimore, MD) sediments were utilized to initiate anaerobic enrichment cultures with polycyclic aromatic
hydrocarbons (PAHs) in the absence of supplementary electron acceptors. Cultures amended with naphthalene and phenanthrene
exhibited sustained, transferable degradation of the PAHs. Bromoethanesulfonic acid, a selective inhibitor of methanogenesis,
inhibited the degradation of 200 μm naphthalene and phenanthrene; molecular characterization based on 16S rRNA sequences confirmed that methanogenic Archaea were eliminated, thus providing evidence that methanogenesis is involved in the degradation pathway.
Revisions requested 16 November 2005; Revisions received 14 December 2005 相似文献
43.
Anaerobic degradation of solid material: importance of initiation centers for methanogenesis, mixing intensity, and 2D distributed model 总被引:2,自引:0,他引:2
Batch anaerobic codigestion of municipal household solid waste (MHSW) and digested manure in mesophilic conditions was carried out. The different waste-to-biomass ratios and intensity of mixing were studied theoretically and experimentally. The experiments showed that when organic loading was high, intensive mixing resulted in acidification and failure of the process, while low mixing intensity was crucial for successful digestion. However, when loading was low, mixing intensity had no significant effect on the process. We hypothesized that mixing was preventing establishment of methanogenic zones in the reactor space. The methanogenic zones are important to withstand inhibition due to development of acids formed during acidogenesis. The 2D distributed models of symmetrical cylinder reactor are presented based on the hypothesis of the necessity of a minimum size of methanogenic zones that can propagate and establish a good methanogenic environment. The model showed that at high organic loading rate spatial separation of the initial methanogenic centers from active acidogenic areas is the key factor for efficient conversion of solids to methane. The initial level of methanogenic biomass in the initiation centers is a critical factor for the survival of these centers. At low mixing, most of the initiation methanogenic centers survive and expand over the reactor volume. However, at vigorous mixing the initial methanogenic centers are reduced in size, averaged over the reactor volume, and finally dissipate. Using fluorescence in situ hybridization, large irregular cocci of microorganisms were observed in the case with minimal mixing, while in the case with high stirring mainly dead cells were found. 相似文献
44.
古丸菌纲(Archaeoglobi)是广古菌门下的纲级分类单元,包含古丸菌(Archaeoglobus)、地丸菌(Geoglobus)和铁丸菌(Ferroglobus)三个属,所属菌株均是严格嗜热厌氧菌,主要分布于海洋、陆地热液系统和油田环境中。Archaeoglobus属下的微生物是一类以硫酸盐、亚硫酸盐或硫代硫酸盐为电子受体代谢生成硫化氢(H2S)的化能自养或氢营养型微生物;而Geoglobus和Ferroglobus的成员则主要还原硝酸盐和铁离子。Archaeoglobi地理分布广泛,在元素生物地球化学循环过程中发挥着重要作用,是目前微生物生态学研究的一个热点。在进化方面,Archaeoglobi菌和产甲烷古菌具有较高的亲缘关系;同时,Archaeoglobi基因组中保留着部分产甲烷途径上的功能基因,最新研究表明部分未培养的Archaeoglobi基因组中含有完整的产甲烷通路。这些证据都表明Archaeoglobi菌的基因组特征可能是产甲烷古菌向硫酸盐还原菌进化的活化石。本文梳理了目前发现的11株Archaeoglobi菌株的生理生化特征和基因组分析结果,从化能自养、化能异养、硫化物呼吸、产乙酸、产甲烷等方面综述了已分离的Archaeoglobi菌的代谢特征,并基于宏基因组信息分析了未培养的Archaeoglobi菌基因组中的潜在代谢功能,为进一步分离培养此类未培养厌氧微生物提供理论指导。 相似文献
45.
内蒙古自治区二连盆地、海拉尔盆地是我国重要的煤层气产区,其中生物成因煤层气是煤层气的重要来源,但复杂物质转化产甲烷相关微生物群落结构及功能尚不清楚。【目的】研究煤层水中的微生物代谢挥发性脂肪酸产甲烷的生理特征及群落特征。【方法】以内蒙古自治区二连盆地和海拉尔盆地的四口煤层气井水作为接种物,分别添加乙酸钠、丙酸钠和丁酸钠厌氧培养;定期监测挥发性脂肪酸降解过程中甲烷和底物的变化趋势,应用高通量测序技术,分析原始煤层气井水及稳定期产甲烷菌液的微生物群落结构。【结果】除海拉尔盆地H303煤层气井微生物不能代谢丙酸外,其他样品均具备代谢乙酸、丙酸和丁酸产生甲烷的能力,其生理生态参数存在显著差异,产甲烷延滞期依次是乙酸<丁酸<丙酸;最大比产甲烷速率和底物转化效率依次是丙酸<乙酸<丁酸。富集培养后,古菌群落结构与煤层气井水的来源显著相关,二连盆地优势古菌为氢营养型产甲烷古菌Methanocalculus (相对丰度13.5%–63.4%)和复合营养型产甲烷古菌Methanosarcina (7.9%–51.3%),海拉尔盆地的优势古菌为氢营养型产甲烷古菌Methanobact... 相似文献
46.
When 50mM FeSO4 was added to cow dung and poultry litter waste which had been processed in daily-fed batch digesters, digesters subsequently unfed showed a faster conversion of substrate and overloaded digesters stabilized within 48 h. Early stabilization of digesters was achieved by adding 20 or 50mM FeSO4 though the latter concentration was faster. When 20mM FeSO4 was added to the daily-fed cow dung and poultry litter waste digesters, it increased methanogenesis by 40% and 42%, respectively, and increased the turnover rate of total solids, volatile solids and volatile fatty acids and the number of methanogens.The authors are with the Department of Microbiology, Osmania University, Hyderabad-500007, India 相似文献
47.
The butyrate-degradingSyntrophospora bryantii degrades butyrate and a propionate-degrading strain (MPOB) degrades propionate in coculture with the hydrogen- and formate-utilizingMethanospirillum hungatii orMethanobacterium formicicum. However, the substrates are not degraded in constructed cocultures with twoMethanobrevibacter arboriphilus strains which are only able to consume hydrogen. Pure cultures of the acetogenic bacteria form both hydrogen and formate during butyrate oxidation with pentenoate as electron acceptor and during propionate oxidation with fumarate as electron acceptor. Using the highest hydrogen and formate levels which can be reached by the acetogens and the lowest hydrogen and formate levels which can be maintained by the methanogens it appeared that the calculated formate diffusion rates are about 100 times higher than the calculated hydrogen diffusion rates. 相似文献
48.
Methanogenesis in Arizona,USA dryland streams 总被引:1,自引:0,他引:1
Jeremy B. Jones Jr. Robert M. Holmes Stuart G. Fisher Nancy B. Grimm Dena M. Greene 《Biogeochemistry》1995,31(3):155-173
Methanogenesis was studied in five streams of central and southern Arizona by examining the distribution of methane in interstitial
water and evasion of methane in three subsystems (hyporheic, parafluvial and bank sediments). In Sycamore Creek, the primary
study site (studied during summer and early autumn), methane content of interstitial water exhibited a distinct spatial pattern.
In hyporheic (sediments beneath the wetted channel) and parfluvial zones (active channel sediments lateral to the wetted channel),
which were well oxygenated due to high hydrologic exchange with the surface stream and had little particulate organic matter
(POM), interstitial methane concentration averaged only 0.03 mgCH4-C/L. Bank sediments (interface between the active channel and riparian zone), in contrast, which were typically vegetated,
had high POM, low hydrologic exchange and concomitantly low dissolved oxygen levels, had interstitial concentration averaging
1.5 mgCH4-C/L. Methane emission from Sycamore Creek, similar to methane concentration, averaged only 3.7 mgCH4-C·m−2·d−1 from hyporheic and parafluvial zones as opposed to 170 mgCH4-C·m−2·d−1 from anoxic bank sediments. Methane in four additional streams sampled (one sampling date during late winter) was low and
exhibited little spatial variation most likely due to cooler stream temperatures. Interstitial methane in parafluvial and
bank sediments of all four streams ranged from only 0.005 to 0.1 mgCH4-C/L. Similarly methane evasion was also low from these streams varying from 0 to 5.7 mgCH4-C·m−2·d−1. The effects of organic matter and temperature on methanogenesis were further examined by experimentally manipulating POM
and temperature in stoppered flasks filled with hyporheic sediments and stream water. Methane production significantly increased
with all independent variables. Methane production is greatest in bank sediments that are relatively isolated hydrologically
and lowest in hyporheic and parafluvial sediments that are interactive with the surface stream. 相似文献
49.
50.
Die-back of Phragmites australis: influence on the distribution and rate of sediment methanogenesis 总被引:4,自引:0,他引:4
Methanogenesis was measured during the summer of 1994, in sediment coresand bulk samples from a Phragmites australis wetland in northern Jutland,Denmark. We compared sediment from healthy reed and dying-back reed, andan open lagoon resulting from die-back. Cores revealed variability withdepth and between sites, with the highest rates coinciding with layers oforganic gyttja, and negligible methane production from the underlying sandbase. Methanogenesis rates in the lagoon and die back sites were higher(up to 100–150 nmol h-1 g-1dry wt. sediment) than in the healthy reed (50–80 nmolh-1 g-1), with the highest rates being recordedfrom May to July. At these times, methanogenesis was markedly temperature-limited; samples incubated at 30 °C anon-limiting temperature, gave rates as high as 200–400nmol h-1 g-1 for the lagoon and die-backareas and 150 nmol h-1 g-1 for the healthyarea. Addition of 8 mM acetate and H2/CO2headspace suggested that both acetate-fermenting andCO2-reducing bacteria were present. Acetate additions suggested some co-limitation by substrate availability, with acetate limitation occurring in the healthy site during July and in the die-back site during August. Lower rates during August, especially in the healthy area, were associated with low water levels which resulted in more oxidized sediments. The data reveal highly variable methanogenesis in the sediment which, when considered with sediment depths, indicates that sites of Phragmites die-back have significantly greater rates of anaerobic mineralization than surrounding healthy wetland, and may be intense sources of methane. 相似文献