HPLC-photodiode array detection analysis of curcuminoids in Curcuma species indigenous to Indonesia

An optimized HPLC method with photodiode array detection was developed and applied to analyse the curcuminoids curcumin, demethoxycurcumin, and bis-demethoxycurcumin in rhizomes of Curcuma mangga Val &. v. Zijp, C. heyneana Val. & v. Zijp, C. aeruginosa Roxb. and C. soloensis Val. (Zingiberaceae), indigenous to Indonesia. The method was validated with an isocratic system, a short run time of 10 min and a baseline separation. The curcuminoid content was 0.18-0.47% for C. mangga, 0.98-3.21% for C. heyneana, 0.02-0.03% for C. aeruginosa and 0.40% for C. soloensis.     

Hedychium menghaiense (Zingiberaceae),a new species from Yunnan,China

Hedychium menghaiense X.Hu & N.Liu,a new species of the Zingiberaceae from Yunnan Province,China,is described and illustrated.The new species is currently known only from its type locality,Nannuoshan Mountain,Menghai County,growing under evergreen broad-leaved forests at altitudes of 1400-1700 m.It is similar to Hedychium forrestii Diels,but differs in its narrower and emarginate labellum folded inwards lengthwise,narrower staminodes,and scarlet stamen.The recognition of the new species is supported by the ...     

Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: Tools for authentication of ginger (Zingiber officinale Rosc)

Phylogenetic analysis and metabolic profiling were used to investigate the diversity of plant material within the ginger species and between ginger and closely related species in the genus Zingiber (Zingiberaceae). In addition, anti-inflammatory data were obtained for the investigated species. Phylogenetic analysis demonstrated that all Zingiber officinale samples from different geographical origins were genetically indistinguishable. In contrast, other Zingiber species were significantly divergent, allowing all species to be clearly distinguished using this analysis. In the metabolic profiling analysis, the Z. officinale samples derived from different origins showed no qualitative differences in major volatile compounds, although they did show some significant quantitative differences in non-volatile composition, particularly regarding the content of [6]-, [8]-, and [10]-gingerols, the most active anti-inflammatory components in this species. The differences in gingerol content were verified by HPLC. The metabolic profiles of other Zingiber species were very different, both qualitatively and quantitatively, when compared to Z. officinale and to each other. Comparative DNA sequence/chemotaxonomic phylogenetic trees showed that the chemical characters of the investigated species were able to generate essentially the same phylogenetic relationships as the DNA sequences. This supports the contention that chemical characters can be used effectively to identify relationships between plant species. Anti-inflammatory in vitro assays to evaluate the ability of all extracts from the Zingiber species examined to inhibit LPS-induced PGE(2) and TNF-alpha production suggested that bioactivity may not be easily predicted by either phylogenetic analysis or gross metabolic profiling. Therefore, identification and quantification of the actual bioactive compounds are required to guarantee the bioactivity of a particular Zingiber sample even after performing authentication by molecular and/or chemical markers.     

Five labdane diterpenoids from the seeds of Aframomum zambesiacum

Five labdane diterpenoids, (3-5), zambesiacolactone A (7) and zambesiacolactone B (8), were isolated from the seeds of Aframomum zambesiacum (Baker) K. Schum., along with five known labdanes and a linear sesquiterpene, nerolidol. Their structures were elucidated by spectroscopic analysis. Their antiplasmodial activity was evaluated in vitro against Plasmodium falciparum. Compound 3 was the most active with an IC(50) value of 4.97 microM.     

中国姜黄属-新种-南昆山莪术

报道姜科姜黄属-新种-南昆山莪术(Curcuma nankunshanensis N.Liu,X.B.Ye&J.Chen).该种与近缘种广西莪术(C kwangsiensis X.X.Chen)的区别在于侧根茎分枝多,叶片阔椭圆状披针形,表面光滑无毛,不育苞片下部白色顶端深紫红色,能育苞片绿色.它与郁金(C aromatica Sahib)的区别在于根茎内面白色,不育苞片下部白色顶端深紫红色,具有种子.     

姜科植物研究进展

姜科植物在种类、外部性状及内部解剖等方面具有高度的复杂性,其传粉方式也显示出了丰富的多样性。因此,姜科植物的研究对姜目乃至单子叶植物的系统与进化研究,都具有十分重要的意义。本文综述了国内外有关姜科植物在分类学、系统学、解剖学、花器官发生学和繁殖生物学等方面的研究和进展,以期能为该类群的系统研究提供一定的帮助。     

Genetic relationships and species authentication of Boesenbergia (Zingiberaceae) in Thailand based on AFLP and SSCP analyses

Genetic relationships of 15 Boesenbergia species were examined using AFLP analysis. A total of 893 fragments were generated from six primer combinations; of these, 99.78% were polymorphic. The mean genetic distance between pairs of taxa ranged from 0.435 to 0.935. The neighbor-joining tree resolved investigated species into two separate lineages (corresponding to species possessing compact or elongated inflorescences) and suggests a rapid radiation in Boesenbergia. In addition, SSCP patterns of the partial psbA–trnH spacer were not overlapping between different species and therefore can be used for authentication of Boesenbergia species. Morphologically similar Boesenbergia longiflora and Boesenbergia sp., which showed three SNPs based on psbA–trnH polymorphism were successfully differentiated by SSCP. Moreover, Boesenbergia curtisii displayed intraspecific ecomorphological variation but exhibited the same SSCP pattern. Therefore, AFLP and SSCP are rapid and cost-effective methods for analysis of genetic similarity and species identification in Boesenbergia.     

天然线性二芳基庚烷类化合物

本文对目前已发现的天然线性二芳基庚烷类化合物在植物中的分布及其结构特征,生源合成途径和药理学研究进展作一简要的综述。     

云南姜科植物的增补与修订

据中国植物志(1981),云南有姜科植物17属, 78种,9变种。其后多篇文章论及一些种属,标本也有较多的增加。本文根据现有的标本与文献资料对云南姜科植物作增补与修订,共涉及16属15种,包括1个新种,7个修定后的异名。云南共计有姜科植物18属,148种和11变种。