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141.
通过对甘农3号、甘农5号和游客紫花苜蓿多元杂交后代选育的36个株系及其亲本的生长、产量、品质等相关指标的测定,采用灰色关联度理论,构造综合评价模型进行供试材料综合评价,筛选出速生12#、速生11#株系为最理想的优良株系,生长高度分别为105.44cm、105.42cm;生长速度分别为1.74cm/d、1.68cm/d;茎叶比分别为0.30、0.35;分枝数分别为23、17;鲜草产量分别为39.99 t/hm2、35.13 t/hm2;粗蛋白含量分别为19.95%、23.89%;相对饲用价值为153.15%、157.02%。多叶2#、速生5#、速生20#、速生21#等4个株系为较理想的优良株系。 相似文献
142.
Manousos Makridakis Maria G. Roubelakis Antonia Vlahou 《Biochimica et Biophysica Acta - Proteins and Proteomics》2013,1834(11):2380-2384
Stem cells have been considered as possible therapeutic vehicles for different health related problems such as cardiovascular and neurodegenerative diseases and cancer. Secreted molecules are key mediators in cell–cell interactions and influence the cross talk with the surrounding tissues. There is strong evidence supporting that crucial cellular functions such as proliferation, differentiation, communication and migration are strictly regulated from the cell secretome. The investigation of stem cell secretome is accumulating continuously increasing interest given the potential use of these cells in regenerative medicine. The scope of the review is to report the main findings from the investigation of stem cell secretome by the use of contemporary proteomics methods and discuss the current status of research in the field. This article is part of a Special Issue entitled: An Updated Secretome. 相似文献
143.
144.
AB Zarafi AM Emechebe AD Akpa O Alabi 《Archives Of Phytopathology And Plant Protection》2013,46(4):261-268
Pearl millet downy mildew (DM) incidence, severity and yield losses of two pearl millet varieties (local and improved) due to the disease were determined in the field. Significant differences in the disease incidence and severity were recorded in the plots sown with metalaxyl-treated seeds and those sown with non-treated seeds, indicating the efficacy of the fungicide on the fungus. Yield losses due to non-treatment of seeds with metalaxyl was 40.88 and 45.39% in a local variety and 43.00 and 18.60% in an improved variety in the 2000 and 2001 cropping seasons respectively. Significant differences between plots sown with metalaxyl-treated and those sown with non-treated seeds were obtained for other yield components such as 1000-grains weight, panicle length and weight. 相似文献
145.
Dr. Renate Gippert Dr. sc Klaus Schmelzer 《Archives Of Phytopathology And Plant Protection》2013,46(6):353-362
Six fungicides, namely Benlate (benomyl) methyl 1-(butylcarbamoyl)benzimidazol-2-ylcarbamate, 50%WP; Dithane M45 (mancozeb) 1,2-ethanediybis carbamodithioato (2-)manganese +1,2-ethanediybis, 20%Mn + 2.5%Zn; Delsene M, (carbendazim + maneb) methyl benzimidazol-2-ylcarbamate, 10% carbendazim + 60% maneb; Rovral (iprodione), 3-(3,5-dichlorophenyl)-N-isopropyl-2-4-dioxoimidazolidine-1-carboxamide, 50%WP; Cupravit (copper oxychloride) 3Cu(OH)2 · CuCl 2 , 50%WP; and Ridomil (metalaxyl) methyl N-(2-methoxyacetyl)-N-(2,6-xylyl-DL-alaminate), 50%WP were evaluated for their efficacy in the control of brown blotch disease of bambara groundnut caused by Colletotrichum capsici (Syd) Butler and Bisby in field trials. Two of the fungicides, Benlate and Delsene M gave significantlty (P = 0.05) better control of the disease and consequently higher grain yield than the other treatments. The performance of Rovral, Dithane M-45 and Ridomil did not differ significantly from each other and Cupravit did not perform significantly better than the check treatment. 相似文献
146.
147.
Mark Q. Martindale 《Invertebrate reproduction & development.》2013,57(2):229-242
Summary In the spiralian embryos studied which display unequal-cleavage at the first two cleavages (either by a polar lobe or an asymmetric cleavage mechanism) the D quadrant is determined at the four cell stage by an unequal segregation of cytoplasmic stuffs. The normal formation of eyes, foot, and shell by overlying micromeres in these forms requires the inductive interaction with the D quadrant before the formation of the third quartet of micromeres. In equal-cleaving spiralians the D quadrant (3D macromere) becomes determined as a result of inductive interactions with first quartet derivatives (animal-vegetal interaction) sometime after the production of the third quartet of micromeres. This paper investigates the exact timing of D quadrant determination and the inductive role of third-order macromeres on the development of micromere derived structures in an equal-cleaving spiralian. Deletions of third-order macromeres, and their derivatives, were performed without rupturing the egg capsule membrane of the Lymnaea embryo with a UV laser microbeam. Virtually normal snails were produced when the 3A, 3B, 3C, or 4D macromere was irradiated. Juvenile snails lacking all mesodermal structures but possessing eyes, foot, and shell were obtained when the mesentoblast (4d) or its progenitor (3D) were deleted. Furthermore, ‘mesoderm-less’ snails were produced by deleting one of the two possible 3D candidates (cross furrow macromeres) as early as 20 min after third quartet formation. These results indicate that the 3D macromere begins to become determined at, or soon after, animal-vegetal interaction; before the 3D macromere becomes visibly distinguishable from the 3B macromere. The results also demonstrate that normal pattern formation in the overlying micromeres does not require the ‘prolonged’ interaction with an asymmetrically positioned 3D macromere. Possible adhesive differences between the 3D macromere and the remaining three macromeres are also revealed. 相似文献
148.
Fu-Ming Chen 《Journal of biomolecular structure & dynamics》2013,31(6):1239-1257
Abstract Hairpin formations of decamers d(CGCG-TA-CGCG), d(CGCG-TG-CGCG), and their m5dC analogs are evidenced by the existence of biphasic absorbance melting profiles in which the lower transition temperature increases with increasing oligomer concentration, whereas the higher melting temperature is concentration independent. The corresponding temperature dependent CD intensity at 285 nm exhibits a maximum around 55°C. These observations are consistent with the interpretation that the lower temperature transition corresponds to the duplex to hairpin transformation while the melting of hairpins into single strands constitutes the higher temperature transition. The CD spectrum of the hairpin conformation appears to be characterized by a couplet with nearly equal positive and negative intensities at 285 and 255 nm, respectively, while a significantly smaller intensity at 285 nm is apparent for the duplex form. The hairpin conformation is suspected to contain a two-nucleotide loop. Titrations with NaCl further suggest that, in contrast to the TA sequence, the TG sequence with wobble base pairing favors Z formation under high salt conditions. 相似文献
149.
M. Yoshikawa 《Journal of fish biology》2013,83(3):588-597
To assess the relationship between growth rate of body mass and sex in the Japanese eel Anguilla japonica in the early life stage; the growth rates of males and females were compared under experimental conditions. The mean growth rate of females was significantly slower than that of males. To assess the relative priority of growth rate and sex, growth was delayed by restricted feeding, resulting in a significantly higher proportion of females in the delayed than in the normal growth group. These findings indicate that the mean growth rate of A. japonica is slower in females than in males in the early life stage around sex determination and differentiation under experimental rearing conditions. Moreover, growth rate probably has priority over sex determination, with slow growth rate increasing the probability of being female. 相似文献
150.
Yumei Li Yuwei Jiang Yiyun Chen Umesh Karandikar Kristi Hoffman Abanti Chattopadhyay Graeme Mardon Rui Chen 《Developmental biology》2013
optix, the Drosophila ortholog of the SIX3/6 gene family in vertebrate, encodes a homeodomain protein with a SIX protein–protein interaction domain. In vertebrates, Six3/6 genes are required for normal eye as well as brain development. However, the normal function of optix in Drosophila remains unknown due to lack of loss-of-function mutation. Previous studies suggest that optix is likely to play an important role as part of the retinal determination (RD) network. To elucidate normal optix function during retinal development, multiple null alleles for optix have been generated. Loss-of-function mutations in optix result in lethality at the pupae stage. Surprisingly, close examination of its function during eye development reveals that, unlike other members of the RD network, optix is required only for morphogenetic furrow (MF) progression, but not initiation. The mechanisms by which optix regulates MF progression is likely through regulation of signaling molecules in the furrow. Specifically, although unaffected during MF initiation, expression of dpp in the MF is dramatically reduced in optix mutant clones. In parallel, we find that optix is regulated by sine oculis and eyes absent, key members of the RD network. Furthermore, positive feedback between optix and sine oculis and eyes absent is observed, which is likely mediated through dpp signaling pathway. Together with the observation that optix expression does not depend on hh or dpp, we propose that optix functions together with hh to regulate dpp in the MF, serving as a link between the RD network and the patterning pathways controlling normal retinal development. 相似文献