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21.
Mauseth James D. Uozumi Yoriko Plemons Brandon J. Landrum James V. 《Journal of plant research》1995,108(4):517-526
Wide-band tracheids are a specialized tracheid type in which an annular or helical secondary wall projects deeply into the
cell lumen. They are short, wide and spindle-shaped, and their bandlike secondary walls cover little of the primary wall,
leaving most of it available for water diffusion. Wide-band tracheids appear to store and conduct water while preventing the
spread of embolisms. They may be the most abundant tracheary element in the xylem, but they are always accompanied by at least
a few vessels. Typically, fibers are absent wherever wide-band tracheids are present. Wide-band tracheids occur in the primary
and secondary xylem of succulent stems, leaves and roots in genera of all three subfamilies of Cactaceae but were not found
in the relictual genusPereskia, which lacks succulent tissues. In the large subfamily Cactoideae, wide-band tracheids occur only in derived members, and
wide-band tracheids of North American Cactoideae are narrower and are aligned in a more orderly radial pattern than those
of South American Cactoideae. Wide-band tracheids probably arose at least three times in Cactaceae. 相似文献
22.
Dr. Herbert Hees Rudolf Leiser Toni Kohler Karl-Heinz Wrobel 《Cell and tissue research》1984,237(1):31-38
Summary The highly coiled testicular artery within the bovine spermatic cord has a constant luminal diameter but a continuously decreasing mural thickness. The pampini form plexus is composed of three interconnected venous networks differing in mesh sizes and calibres. The large veins of the first network display pouches and permanent constrictions, which may serve as throttle devices. The constitutents of the third network are venules or venous capillaries with diameters between 10 and 20 m; they favor a periarterial position or even occupy the media-adventitia border of the testicular artery. All plexus veins are devoid of valves. The existence of true arteriovenous anastomoses between smaller branches of the testicular artery and plexus veins was established by serial sections. The vascular morphology of the spermatic cord is discussed with special attention to a postulated venous-arterial steroid transfer in this region.Supported by the Deutsche Forschungsgemeinschaft and the Stiftung zur Förderung der wissenschaftlichen Forschung an der Universität Bern 相似文献
23.
Endogenous, free indol-3yl-acetic acid (IAA) levels were measured in the main stem in the 10-year-old cambial zone, in the adjoining differentiating xylem, and in the adjoining mature xylem of 15–20-year-old Pinus contorta Dougl. by single-ion-current monitoring, combined gas chromatography — mass spectrometry, on several dates from early spring to early winter. Microscopy was used to determine the state of cambial activity on each harvest date. The IAA levels were found to be nearly constant at 1 g g-1 DW in the cambial zone from March to July, then to increase to near 2 g g-1 DW during the remainder of the growth season. No clear correlation was evident between number of fusiform cells per radial file and IAA content in the cambial zone. By contrast, the IAA content in differentiating xylem was higher than that in the adjoining meristematic zone on all harvest dates and also exhibited marked seasonal variation, peaking near 16 g g-1 DW in mid summer, and declining to 1 g g-1 DW in autumn. In mature xylem, IAA levels were very low and showed negligible variation. The fresh weight to dry weight ratio of differentiating xylem was greater than that of the cambial zone, and greater in the cambial zone than in mature xylem. 相似文献
24.
Prof. Dr. Dr. Karl-Heinz Wrobel Fred Sinowatz Roswitha Mademann 《Cell and tissue research》1981,217(2):289-310
Summary The intertubular stroma of the bovine testis is composed of narrow strands between two adjacent tubules and larger tri- and quadrangular interstices between three to four tubules. The latter contain the majority of Leydig cells, larger blood vessels and testicular lymph vessels. Ley dig cells occur in groups or cords, not every cell being in close contact to a capillary, lymph vessel or venule. Between adjacent Leydig cells intercellular canaliculi and gap junctions are frequently encountered. Bovine Leydig cells are further characterized by an abundance of ribosome-associated endoplasmic reticulum, by mitochondria often containing crystalloid structures and displaying both tubular and lamelliform cristae, as well as by a relative paucity of lipid droplets and lysosomes. Independent of the size of intertubular lymph vessels their walls consist only of an endothelium of varying thickness, no typical basal lamina or associated musculature being present. The interstitial surface of the endothelium sends anchoring cytoplasmic pedicles into the subjacent ground substance and collagen fibrils. Among occasional plasma cells, mast cells and mononuclear leucocytes, a regular constituent of the intertubular region studied is a population of electron-lucid, irregularly shaped cells (light intercalated cells = LIC) with slender, pleomorphic processes. These cells are believed to be involved in testicular androgen storage and distribution.Supported by a grant from the Deutsche Forschungsgemeinschaft 相似文献
25.
Ultrastructure of arterioles in the cat brain 总被引:2,自引:0,他引:2
Summary A total of 110 arterioles were examined in the brains of cats; different sites were studied including the cortex, putamen, pons and crus cerebri. No internal elastic laminae were seen in the subendothelial space, although occasional fragments of elastic material were present in the larger arterioles. The media was composed of one, two or three layers of smooth muscle cells which interlocked in such a way that the vessel wall thickness was constant. Numerous tight junctions were seen between adjacent smooth muscle cells and between the endothelium and smooth muscle cells. Apart from the usual cell organelles, the smooth muscle cells of arterioles had numerous dense patches on the cell surface. The structure of the adventitia varied according to the diameter of the vessel and the site in the brain; it contained adventitial cells, bundles of collagen fibres and nerve fibres. Innervation of arterioles was more constant in the brain stem than in the cortex. Metarterioles had less specialised, atypical smooth muscle cells, a discontinuous media and numerous, extensive myoendothelial tight junctions; they were not innervated by nerve fibres. The diameter of metarterioles was less than 10 m whereas that of arterioles was 10–45 m. The possible functional aspects of arteriolar innervation are discussed. 相似文献
26.
Richard P. C. Johnson 《Planta》1977,136(3):187-194
Vascular bundles of petioles below wilted leaves of Nymphoides peltata (S.G. Gmel. O. Kuntze) were frozen intact and freeze-fractured for electron microscopy. Cell walls in them appeared drawn in against the helical thickenings of xylem vessels. By contrast, walls round vessels which had been frozen in vascular bundles below turgid leaves, and walls round vessels which had been fixed, embedded and sectioned, were straight or bulged outwards slightly. Walls bulged outwards slightly also from cut vessels filled with sucrose solution before freezing. Movement of vessel walls could produce the clicks audible when water cavitates in vessels, and might explain a variable resistance to the flow of water through plants. 相似文献
27.
Summary Nitrate reductase (EC 1.6.6.2) activity (NRA), as measured by an in vivo assay, is present in needle leaves and mycorrhizal fine root tips of adult Norway spruce [Picea abies (L.) Karst.] in at least equal amounts on a fresh weight basis, in both adult and 5-year-old trees. NRA could also be demonstrated in trunk wood of deroted trees after fertilization with 5 mM
, exhibiting a longitudinal profile in the trunk. Inducibility in needles can more efficiently be achieved by NO2 (100 g·m-3) than by 5 mM nitrate, which is effective only in root-amputated trees. A remarkably high level of needle-NRA in unfertilized trees, which are characterized by a very low level of nitrate in the xylem sap, suggests that NRA in spruce needles may in part be constitutive. Organic-N is a major nitrogen source for the needles even in root-amputated trees, indicating pronounced exchange processes between ray parenchyma and trunk xylem, which in turn are modified by the nitrogen source fed to the trunk stump. Intact trees exhibit a very similar amino acid composition of the xylem sap, regardless of whether
or
has been fed. The amino acid pattern of the needles is not thrown out of balance by flooding with
and
, which occurs in fertilized derooted trees. This indicates a distinct potential for homoeostasis of nitrogen entrance-metabolism (i.e. NRA and glutamine synthetase activity) in the needles. In the ectomycorrhiza/fine root-system (EMC), marked differences in NRA were observed depending on root-tip diameter and along the longitudinal profile of the fine roots. EMC-nitrate reductase is strongly enhanced by
. Needle-NRA exhibits a circannual rhythm. An early summer maximum is followed by a December minimum. This activity pattern matches well the transitory increase of soluble nitrogen in spring and the total protein maximum in winter. In an indirect way assimilatory NRA may well contribute to nitrogen overfertilization (by consumption of NOX) as one possible cause of the contemporary decline of spruce populations. 相似文献
28.
Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels
Thomas J. Goodwin J. Milburn Jessup David A. Wolf 《In vitro cellular & developmental biology. Animal》1992,28(1):47-60
Summary A new low shear stress microcarrier culture system has been developed at NASA’s Johnson Space Center that permits three-dimensional
tissue culture. Two established human colon adenocarcinoma cell lines, HT-29, an undifferentiated, and HT-29KM, a stable,
moderately differentiated subline of HT-29, were grown in new tissue culture bioreactors called Rotating-Wall Vessels (RWVs).
RWVs are used in conjunction with multicellular cocultivation to develop a unique in vitro tissue modeling system. Cells were
cultivated on Cytodex-3 microcarrier beads, with and without mixed normal human colonic fibroblasts, which served as the mesenchymal
layer. Culture of the tumor lines in the absence of fibroblasts produced spheroidlike growth and minimal differentiation.
In contrast, when tumor lines were co-cultivated with normal colonic fibroblasts, initial growth was confined to the fibroblast
population until the microcarriers were covered. The tumor cells then commenced proliferation at an accelerated rate, organizing
themselves into three-dimensional tissue masses that achieved 1.0- to 1.5-cm diameters. The masses displayed glandular structures,
apical and internal glandular microvilli, tight intercellular junctions, desmosomes, cellular polarity, sinusoid development,
internalized mucin, and structural organization akin to normal colon crypt development. Differentiated samples were subjected
to transmission and scanning electron microscopy and histologic analysis, revealing embryoniclike mesenchymal cells lining
the areas around the growth matrices. Necrosis was minimal throughout the tissue masses. These data suggest that the RWV affords
a new model for investigation and isolation of growth, regulatory, and structural processes within neoplastic and normal tissue. 相似文献
29.
Are there seasonal variations of trace element concentrations (Cd,Pb, Zn) in wood of Fagus trees in Germany? 总被引:1,自引:0,他引:1
Concentrations of Cd, Pb and Zn were determined in stem wood of beech trees (Fagus sylvatica L.) from 3 sites in northern Germany. Distinct radial distribution patterns of the elements were observed in the xylem. Concentrations of Cd and Pb increased from the youngest, outermost annual rings towards the center of the stem. With Zn intermediate concentrations were observed in the sapwood and higher levels at the center of the stem.Temporal and spatial stability of such distribution patterns in the trunks was investigated. Wood samples taken from the same individual tree in different months of the year were analysed. Marked seasonal variations of mineral concentrations were observed. Also the shape of the distribution patterns of the elements varied with the season. Such variations were larger than those observed with samples taken simultaneously from different sides of the trunk. Furthermore, Pb concentrations in the stem showed variations with height above ground.The results indicate, that radial distribution patterns of Cd, Pb and Zn in xylem rings of beech are not stable. Biomonitoring trace element pollution levels by analysis of beech wood is, thus, questionable. To obtain a reliable historical record of pollution from tree rings, the distribution patterns should be stable over a long period of time. This basic requirement of the dendroanalytical method does not hold for the examined beech. Still, with other tree species and under more favourable conditions the dendroanalytical biomonitoring method may prove valuable.Presented as symposium paper at the V International Congress of Ecology, Yokohama, August 23–30, 1990. 相似文献
30.
The technique of EDTA-enhanced phloem exudation (King and Zeevaart, 1974: Plant Physiol. 53, 96–103) was evaluated with respect to the collection and identification of amino acids exported from senescing wheat leaves. Whilst the characteristics of the exudate collected conform with many of the accepted properties of phloem exudate, unexpectedly high molar proportions of phenylalanine and tyrosine were observed. By comparing exudation into a range chelator solutions with exudation into water, the increased exudation of phenylalanine and tyrosine relative to the other amino acids occurring when ethylene-diaminetetracetic acid was used, was considered to an artefact.In plants thought to be relying heavily on mobilisation of protein reserves to satisfy the nitrogen requirements of the grain, the major amino acids present in flag-leaf phloem exudate were glutamate, aspartate, serine, alanine and glycine. Only small proportions of amides were present until late in senescence when glutamine became the major amino acid in phloem exudate (25 molar-%). Glutamine was always the major amino acid in xylem sap (50 molar-%).The activities of glutamine synthetase (EC 6.3.1.2), glutamate synthase (EC 1.4.7.1), glutamate dehydrogenase (EC 1.4.1.3) and asparagine synthetase (EC 5.3.5.4) were measured in the flag leaf throughout the grain-filling period. Glutamine synthetase and glutamate-synthase activities declined during this period. Glutamate-dehydrogenase activity was markedly unchanged despite variation in the number of multiple forms visualised after gel electrophoresis. The activity of the enzyme reached a peak only very late in the course of senescence of the flag leaf. No asparagine-synthetase activity could be detected in the flag leaf during the grain-filling period.II. Peoples et al. (1980) 相似文献