新疆天山一号冰川地区十种藓类植物叶尖的微形态观察

在光学显微镜和扫描电镜下,对新疆天山一号冰川地区生长的10种藓类植物的叶尖（毛尖）的形态、齿（刺毛）、疣、角质层纹饰等微结构进行观察,其中电镜观察结果均为首次报道。结果表明：10种藓类植物叶尖的顶尖细胞和边缘细胞大部分都是透明的,并且细胞壁厚,细胞腔大;而叶尖边缘内卷、粗糙,细胞壁厚,干时细胞壁上下或侧面凹陷,其上有较多的小孔,这些都是明显耐旱特征,有利于水分的吸收及抵御长期寒冷、反射太阳辐射对其伤害,叶尖的类型对苔藓植物科、属级的分类意义不大,但其微形态如叶尖细胞及边缘细胞的形状、数目、细胞壁的凹陷程度以及其上角质层纹饰、乳突的微形态在同属的不同种之间存在明显差异,对于属下种间的鉴定具有一定的分类学价值。     

新疆天山一号冰川地区12种藓类植物叶形态结构研究

采用石蜡切片和扫描电镜的方法,对新疆天山一号冰川地区的12种藓类植物叶片结构及叶表面微形态进行了观察。结果显示,不同苔藓植物叶细胞、中肋、细胞壁、细胞表面等各项指标在类型、大小、分布、干燥时细胞壁的凹陷程度以及表面角质层纹饰和小孔形态、分布等都明显不同,这些微形态特点不仅在藓类植物属下种间的区分上具有分类学意义,同时也反映出藓类植物叶片为保水抗旱,抵御长期寒冷、多风和强光照射等恶劣环境对其的伤害而形成的生存策略。本研究结果为苔藓植物在极端环境地区的生态学功能研究提供了重要的科学依据。     

亚麻耐盐性愈伤组织的生理生化特性

以亚麻(双亚5号)耐盐愈伤组织为材,以同种亚麻普通愈伤组织为对照,分别经含0~250 mmol·L^-1 NaC1的培养基培养20 d后,比较含水量、MDA含量、POD酶活、SOD酶活、EST同工酶谱、POD同工酶谱及SOD同工酶谱。结果表明：亚麻耐盐组愈伤组织与对照组愈伤组织在多方面存在明显差异,前者含水量均高于后者;丙二醛含量也高于后者,但变化幅度不大;POD酶活均高于对照组;酯酶同工酶谱不同于对照组,酯酶酶活量均高于对照组;POD同工酶谱比对照组多一条带,且酶活均高于对照组;SOD同工酶谱与对照组一样多,但酶活均高于对照组,且平稳。这为进一步筛选亚麻耐盐突变细胞系奠定了良好的基础。     

寒地早粳新品种龙糯2号的选育与评价

水稻优良亲本的选择是选育新品种的关键,经过17年的株系选择、抗性选育、鉴定和品质检验等研究,育成了适宜寒地种植的集优质、高产、抗病、抗寒等于一体的水稻新品种.龙糯2号的育成说明利用优异核心种质,选择适宜寒地稻作的杂交亲本,采用有效的选育方法,利用多学科协作研究,通过基因重组是选育寒地早粳新品种的有效手段.     

实时荧光定量PCR检测RSV胁迫下抗病、感病水稻中与脱落酸相关基因的差异表达

采用实时荧光定量PCR方法测定了水稻条纹病毒(Rice stripe virus,RSV)胁迫下抗性不同品种水稻中与脱落酸相关基因的mRNA转录水平变化.结果表明:感病品种武育梗3号中WGPI、OsGASA2、Polcalcin、OsCBIA、Myb和OsCIPK15基因表达水平均上调,上调比率分别为4.96、5.17、2.01、5.17、12.04和7.84.而抗病品系KT 95-418中,OsGASA2和OsCIPK15基因表达水平下调,下调比率分别为1/5.40和1/2.08;Polcalcin和Myb基因表达水平上调,上调比率分别为4.20和3.86;WGPI和OsCBIA表达量变化不明显.这些结果表明,RSV胁迫能诱导脱落酸相关基因表达量的变化,并且在抗病、感病水稻品种中的表达特征不同,从而提示植物激素脱落酸可能调控了RSV胁迫条件下相关基因的表达.     

非繁殖期大仓鼠对同种气味的反应和个体间的行为关系

在实验室通过对陌生成年大仓鼠（Ｃｒｉｃｅｔｕｌｕｓｔｒｉｔｏｎ）非繁殖期的气味偏好,性别选择和两鼠间的行为关系的实验研究表明,用Ｙ－型迷宫进行选择测试时,与空白组比较,大仓鼠了同种个体鼠垫的气味,在雌雄巢垫气味之间,雌鼠偏好雄性巢垫的气味,雄鼠不表现性别偏好,与空白组比较,被试鼠选择同种个体;在雌雄个体之间,雄性大仓鼠选择同性个体,雌性不表现性别偏好,在观察箱内,无论同性相遇还是异性相遇,两鼠间攻     

Correlations of IL-23R gene polymorphism with clinicopathological characteristics and prognosis of hepatocellular carcinoma patients after interventional therapy

In this study, we summarized the association of IL-23R gene polymorphisms with hepatocellular carcinoma (HCC). A total of 270 HCC patients were selected as HCC group, and 251 healthy individuals served as the control group. PCR-RFLP was performed to detect IL-23R gene polymorphism, including rs17375018 and rs11805303. Survival rate and risk factors of HCC were identified. The findings suggested that IL-23R rs17375018 was correlated with genetic susceptibility to HCC, and GC haplotype was closely linked with the risk factors of HCC. Moreover, rs17375018 polymorphism was related to portal vein tumor thrombus (PVTT) and alcohol consumption in HCC patients, while prognosis was better in HCC patients with AA genotype of rs17375018 polymorphism. Lastly, GG genotype in rs17375018, PVTT and TNM stage III and IV were identified as independent risk factors for HCC. In conclusion, IL-23R rs17375018 polymorphism might serve as a prognostic factor in patients with HCC after interventional therapy.     

RanBPM, Muskelin, p48EMLP, p44CTLH, and the armadillo-repeat proteins ARMC8alpha and ARMC8beta are components of the CTLH complex

Ran-binding protein in microtubule organising centre (RanBPM) was originally isolated as a protein that binds to the small GTPase Ran. Recently our group and other groups reported that RanBPM was associated with several proteins and composed a large protein complex. Here, we used tandem MS with an antibody against RanBPM to purify this complex from a soluble extract of HEK293 cells: we identified Muskelin, p48EMLP, p44CTLH, and the novel armadillo-repeat proteins ARMC8alpha and ARMC8beta as components. In RanBPM, Muskelin, p48EMLP, and p44CTLH we found LisH/CTLH motifs, which are present in proteins involved in microtubule dynamics, cell migration, nucleokinesis, and chromosome segregation. We renamed the 20S large protein complex the CTLH complex. The N-terminal 364 amino acids of ARMC8alpha and ARMC8beta were completely conserved, suggesting that these proteins are probably alternatively spliced products from the same gene. We confirmed the in vivo association of each component by co-immunoprecipitation assays with Cos-7 cells in which these components were exogenously overexpressed. A pull-down assay with bacterially-expressed Twa1 revealed binding of each in vitro-translated component to Twa1. Finally, we confirmed the cellular localization of these proteins. Taken together, our results reveal that RanBPM, ARMC8alpha, ARMC8beta, Muskelin, p48EMLP, and p44CTLH form complexes in cells.