陕西省维管植物名录(2021版)

陕西省位于黄土高原和秦巴山区的核心地带, 复杂多样的生态地理环境孕育了丰富的植物种类。为了及时总结并展现陕西省植物物种多样性与分布信息, 基于已公开发表的论著, 本文更新构建了2021版陕西省维管植物名录。结果表明, 陕西省野生、归化及栽培维管植物有5,161种及种下分类群, 包含209科1,316属4,701种, 种下分类群有112亚种488变种17变型。物种数比上一版名录增加了214种, 主要是近年来发现的新分布野生植物, 也有少量的新物种和外来入侵植物。新版名录中科的范围界定与编排采用了以分子系统学结果为主要依据的新的分类系统, 并以Excel格式数据集的形式发表, 便于公众获取并检索利用。本文建议应持续开展野外考察工作, 尤其是加强对巴山地区的调查, 对外来入侵植物的监测也不容忽视。     

Three-dimensional structure determined for a subunit of human tRNA splicing endonuclease (Sen15) reveals a novel dimeric fold

Splicing of eukaryal intron-containing tRNAs requires the action of the heterotetrameric splicing endonuclease, which is composed of two catalytic subunits, Sen34 and Sen2, and two structural subunits, Sen15 and Sen54. Here we report the solution structure of the human tRNA splicing endonuclease subunit HsSen15. To facilitate the structure determination, we removed the disordered 35 N-terminal and 14 C-terminal residues of the full-length protein to produce HsSen15(36-157). The structure of HsSen15(36-157), the first for a subunit of a eukaryal splicing endonuclease, revealed that the protein possesses a novel homodimeric fold. Each monomer consists of three alpha-helices and a mixed antiparallel/parallel beta-sheet, arranged in a topology similar to that of the C-terminal domain of Methanocaldococcus jannaschii endonuclease. The dimeric interface is dominated by a beta-barrel structure, formed by face-to-face packing of two, three-stranded beta-sheets. Each of the beta-sheets results from reciprocal parallel pairing of one beta-strand from one subunit with two other beta-strands from the symmetric subunit. The structural model provides insights into the functional assembly of the human tRNA splicing endonuclease.     

Towards elucidation of the mechanism of UV1C, a deoxyribozyme with photolyase activity

Among the unexpected chemistries that can be catalyzed by nucleic acid enzymes is photochemistry. We have reported the in vitro selection of a small, cofactor-independent deoxyribozyme, UV1C, capable of repairing thymine dimers in a DNA substrate, most optimally with light at a wavelength of >300 nm. We hypothesized that a guanine quadruplex functioned both as a light antenna and an electron source for the repair of the substrate within the enzyme-substrate complex. Here, we report structural and mechanistic investigations of that hypothesis. Contact-crosslinking and guanosine to inosine mutational studies reveal that the thymine dimer and the guanine quadruplex are positioned close to each other in the deoxyribozyme-substrate complex, and permit us to refine the structure and topology of the folded deoxyribozyme. In exploring the substrate utilization capabilities of UV1C, we find it to be able to repair uracil dimers as well as thymine dimers, as long as they are present in an overall deoxyribonucleotide milieu. Some surprising similarities with bacterial CPD photolyase enzymes are noted.     

Effect of Forest Fire Suppression on Buff-Breasted Flycatchers

ABSTRACT Buff-breasted flycatchers (Empidonax fulvifrons) are rare in the United States due to a >90% reduction in breeding distribution. Previous authors have implicated fire suppression in montane woodlands as the underlying cause of population declines and range contraction. We examined the effect of fire suppression on population declines of buff-breasted flycatchers by comparing both presence and abundance of flycatchers in areas with and without evidence of recent fire in 9 mountain ranges in southern Arizona, USA. We also replicated previous survey efforts conducted in 1980–1983 and 1995–1996 to determine population trajectory. Twenty-two (63%) of 35 survey routes had negative trends, and the average slope of the declines was −0.105 (10.5% annual decline). The number of buff-breasted flycatchers detected at a survey point was positively associated with severity of recent fires, and flycatchers were particularly associated with areas that had evidence of high-severity surface fire. However, we failed to detect flycatchers in 5 canyons that recently burned, which suggests one or more of the following: 1) fire suppression is not the cause (or is not the main cause) of population decline and range contraction, 2) flycatchers do not colonize burned areas until >10 years postfire, 3) low- or medium-severity fires are insufficient to make fire-suppressed areas suitable for breeding flycatchers, or 4) local recruitment and immigration are insufficient to allow buff-breasted flycatchers to expand into recent firerestored areas. Continued suppression of high-severity forest fires in the southwestern United States may eventually result in the extirpation of buff-breasted flycatchers. A landscape that includes a mosaic of recently burned and unburned forest patches appears to be most suitable for buff-breasted flycatchers. Prescribed burning is unlikely to help restore flycatcher populations unless burns are of high severity, conditions typically avoided during prescribed burns for safety reasons.     

Two new species of Kazachstania that form ascospores connected by a belt-like intersporal body: Kazachstania zonata and Kazachstania gamospora

Four strains of ascomycetous yeasts were isolated from samples collected at two locations in southern Japan. The strains formed two warty ascospores that were joined together by an intersporal body appearing as a belt. Phylogenetic analysis of rRNA gene nucleotide sequences indicated that the strains represented two new and closely related species of the genus Kazachstania. Isolates of one of the species were from Miyazaki Prefecture and those of the other species were from the Iriomote Islands. Genetic separation of the two species was further confirmed by DNA-DNA reassociation, which gave values of 63.3-78.1%, and from interspecific crosses, which gave nonviable ascospores. On the basis of these data, the isolates from Miyazaki Prefecture are described as Kazachstania zonata sp. nov. [type strain NBRC 100504=CBS 10326, mating types NBRC 101821 (+), NBRC 101822 (-)], and the isolates from the Iriomote Islands are described as Kazachstania gamospora sp. nov. [type strain NBRC 11056=CBS 10328, mating types NBRC 101825 (+), NBRC 101826 (-)].     

Differential interactions of 5-(4-piperidyl)-3-isoxazolol analogues with insect γ-aminobutyric acid receptors leading to functional selectivity

γ-Aminobutyric acid (GABA) receptors (GABARs) mediate fast inhibitory synaptic transmission and are also targets for drugs and insecticides. To better understand the molecular interactions of ligands with the orthosteric sites of GABARs, we examined 4-aryl/arylalkyl-5-(4-piperidyl)-3-isoxazolol, 4-aryl-5-(4-piperidyl)-3-isothiazolol, and 5-aryl-4-(4-piperidyl)-1-hydroxypyrazole for their antagonism with regard to three insect GABARs. The 3-isoxazolol was preferable to the 3-isothiazolol and 1-hydroxypyrazole in antagonism to common cutworm and housefly GABARs. Of the tested analogues, 4-(3-biphenylyl)-5-(4-piperidyl)-3-isoxazolol (2a) displayed the greatest antagonism for common cutworm and housefly GABARs, with IC₅₀ values of 3.4 and 10.2 μM, respectively. In contrast to the antagonism of the two GABARs, 2a showed partial agonism for the case of small brown planthopper GABARs, with an EC₅₀ value of 31.3 μM. Homology models and docking simulations revealed that a cation-π interaction between an analogue and an Arg residue in loop C or E of the orthosteric site is a key component of antagonism. This specific phenomenon was lacking in the interactions between 2a and the orthosteric site of small brown planthopper GABARs. These findings provide important insights into designing and developing novel drugs and insecticides.     

厚壳贻贝抗氧化酶活性和脂质过氧化物含量对Cd和Aroclor 1254胁迫的响应

以厚壳贻贝(Mytilus coruscus)为目标生物,研究了不同浓度Cd2+(0.194、0.388、0.775 mg·L-1)、Aroclor 1254(0.005、0.01、0.05 mg·L-1)单一和复合胁迫对其消化盲囊抗氧化酶活性及脂质过氧化产物丙二醛(MDA)含量的影响。结果表明:(1)单一和复合胁迫均可导致厚壳贻贝抗氧化酶的活性先升高后抑制。高浓度组(0.775 mg·L-1Cd2+、0.05mg·L-1Aroclor 1254)在单一胁迫第1天时,抗氧化酶的活性即达峰值。抗氧化酶活性的下降幅度和速率与胁迫物质作用时间和浓度呈正相关。(2)SOD、GSH-Px对胁迫的敏感性高于CAT。(3)各胁迫处理组MDA含量随胁迫时间延长均显著升高(P0.01)。单一胁迫的效应与剂量和时间呈正相关。(4)复合胁迫的效应强于单一胁迫。推测高于环境水平的Cd2+、Aroclor 1254对厚壳贻贝可产生明显氧化胁迫,抗氧化防御系统可以作为海洋环境重金属-有机物复合污染监测的潜在生物标志物。     

子午岭地区草本药用植物种间联结性研究

该研究以子午岭地区桥山林区最常见的几种药用植物为研究对象,通过种间联结系数(AC)、Ochai指数(OI)、Jaccard指数(JI),结合Pearson系数和以及χ2统计量对研究区内的药用草本植物两两之间及药用草本植物与本区常见优势木本植物间的种间联结性进行分析,以了解子午岭地区野生药用植物资源的分布。结果显示:(1)药用草本物种间白头翁与苦参、黄精与沙参显示出极显著的正联结关系,而穿龙薯蓣与刺儿菜、翻白草、祁州漏芦、茵陈蒿、远志,刺儿菜与玉竹,翻白草与黄精,黄精与茵陈蒿则表现出极显著的负联结关系。(2)木本植物与药用草本植物的关系因物种不同而异,其中刺槐与刺儿菜、辽东栎与穿龙薯蓣、辽东栎与玉竹、辽东栎与黄精、白桦与穿龙薯蓣均具有极显著的正联结关系;而刺槐与穿龙薯蓣、玉竹,辽东栎与刺儿菜、翻白草、茵陈蒿、苦参,槲栎与龙牙草、茵陈蒿,白桦与天南星、茵陈蒿,油松与益母草,野胡桃与黄精均具有极显著的负联结关系。(3)基于物种关联性的不同,将药用草本植物划分为3个生态种组。研究认为,种间联结性的研究结果可以反映物种的生理生态特性,对该区药用经济植物的资源开发与利用具有一定参考价值。     

Four new species and additional records of Domene and Lathrobium from the Dayao Mountains,southern China

Material of the paederine genera Domene Fauvel, 1873 and Lathrobium Gravenhorst, 1802 from the Dayao Mountains, southern China, is examined. Eight species are identified, three of them described previously and five undescribed. Four species are described and illustrated for the first time: Domene hei Peng & Li, sp. n., Lathrobium jinxiuense Peng & Li, sp. n., Lathrobium kuan Peng & Li, sp. n. and Lathrobium leii Peng & Li, sp. n. One probably undescribed species of Lathrobium remains unnamed.     

The Tetramorium squaminode species group (Hymenoptera,Formicidae) in the Arabian Peninsula,with a new record from the Kingdom of Saudi Arabia and keys to Arabian species

The Arabian species of the Tetramorium squaminode-group are treated. Tetramorium squaminode Santschi, 1911 is recorded for the first time from the Kingdom of Saudi Arabia and the Arabian Peninsula. Keys to the two Arabian species of the Tetramorium squaminode-group, Tetramorium latinode Collingwood & Agosti, 1996 and Tetramorium squaminode, based on worker and queen castes, are given and a regional distribution map is provided. Notes on habitats of Tetramorium squaminode are presented.