全文获取类型
收费全文 | 1090篇 |
免费 | 18篇 |
国内免费 | 36篇 |
专业分类
1144篇 |
出版年
2023年 | 10篇 |
2022年 | 17篇 |
2021年 | 20篇 |
2020年 | 19篇 |
2019年 | 21篇 |
2018年 | 20篇 |
2017年 | 20篇 |
2016年 | 8篇 |
2015年 | 17篇 |
2014年 | 49篇 |
2013年 | 176篇 |
2012年 | 66篇 |
2011年 | 55篇 |
2010年 | 26篇 |
2009年 | 59篇 |
2008年 | 45篇 |
2007年 | 34篇 |
2006年 | 40篇 |
2005年 | 41篇 |
2004年 | 24篇 |
2003年 | 17篇 |
2002年 | 25篇 |
2001年 | 10篇 |
2000年 | 14篇 |
1999年 | 10篇 |
1998年 | 14篇 |
1997年 | 14篇 |
1996年 | 7篇 |
1995年 | 18篇 |
1994年 | 24篇 |
1993年 | 15篇 |
1992年 | 15篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1989年 | 15篇 |
1988年 | 8篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 11篇 |
1984年 | 23篇 |
1983年 | 21篇 |
1982年 | 10篇 |
1981年 | 18篇 |
1980年 | 14篇 |
1979年 | 11篇 |
1978年 | 10篇 |
1977年 | 10篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1973年 | 4篇 |
排序方式: 共有1144条查询结果,搜索用时 15 毫秒
11.
Scolecopteris Zenker, a kind of anatomically-preserved fertile foliage of Late Paleozoic Marattiales, has been well studied in Euramerican Flora. It is composed of 28 species which can be divided into four forms (groups) mainly based on modified or umodified pinnules, the variation of the outer facing sporangial walls, and with or without a prominent central parenchyma area. In contrast, Scolecopteris Zenker in Cathaysian Flora has been poorly studied so far, and only one species S. sinensis Zhao, was reported in 1991 which was considered as a member of Minor Group. The paper reports a second species of Scolecopteris, i.e.S, shanxiensis sp. nov., which differs from the above four groups in that its outer facing wall of the sporangia is thick at the base and top (2 ~ 3 layers of cells), and a little thinner ( 1~2 layers of cells) at the midlevel of the synangia. So a new group, Shanxiensis group, is set for the new species. The other characteristics of Shanxiensis group is comparable with Minor group. The new species comes from the coal balls in Coal Seam No. 7 in the upper part of Taiyuan Formation (early Early Permian) from Taiyuan, Shanxi, China. The identification of Scolecopteris shanxiensis sp. nov. :The fertile pinnule probably peeopterids, 5.5 ~ 6.0 mm in length and 2.0 ~ 2.2 mm in width. The lateral extensions of the lamina of the pinnule bend abaxially and above the synangia. The synangia arrange along the sides of the midrib of the pinnnle and there are about 10 synangia in each row. The synangium is elliptical in longitudinal section and radial in cross section, 0.7 ~ 0.8 mm in height and 0.6 ~ 0.7 mm in diameter. Each synangium has 5-7 (mostly 6) fusiform sporangia fused at the base and attached to the top of the synangial pedicel. The outer facing wall of the sporangia consists of 2~3 layers of cells at the base and becomes thinner at the midlevel (1~2 layers of cells), and at the top of the synangia the wall become thicker again. The cells of the outer facing wall of the sporangia are elongate in the longitudinal sections. The lateral and inner facing walls of the sporangia are one cell thick. The synangial pedicel is small. Spores in situ are small, generally 11~14 µm in diameter, spherical or rounded-triangular, trilete and smooth-walled. 相似文献
12.
The fig (Ficus L.) infructescence, called syconium, is a receptacle with an apical opening, the ostiole, closed by bracts. The ostiolar bracts produce an exudate, which is rather conspicuous in some species. It has not been histochemically analyzed yet, and the structures responsible for its production are still unknown. Some wild growing species of Ficus from Brazil produce high amounts of this ostiolar exudate. Ficus enormis (Mart. ex Miq.) Miq. grows as trees or shrubs in the Atlantic rainforest. Our goal was to identify the secretory structures present in the inflorescence and, to characterize histochemically the ostiolar tissues and exudates. Syconia samples of F. enormis were processed and stained according to the usual techniques in plant anatomy. The morphological analysis revealed different types of bracts, one type specialized in secretion, another showing transitional characteristics between secretory and non-secreting bracts, and a third one being non-secreting. They are designated as secretory ostiolar bracts, transitional bracts and wall bracts. The floral bracteoles, digital-shaped colleters present in the ostiole, at the syconium axis and at the flower receptacle, were also analyzed. All have similar structure, like finger-shaped secretory trichomes. The colleters present among ostiolar bracts may contribute to production and composition of the ostiole exudate. 相似文献
13.
14.
The measurement of glomerular filtration rate (GFR) is the gold standard in kidney function assessment. Currently, investigators determine GFR by measuring the level of the endogenous biomarker creatinine or exogenously applied radioactive labeled inulin (3H or 14C). Creatinine has the substantial drawback that proximal tubular secretion accounts for ~50% of total renal creatinine excretion and therefore creatinine is not a reliable GFR marker. Depending on the experiment performed, inulin clearance can be determined by an intravenous single bolus injection or continuous infusion (intravenous or osmotic minipump). Both approaches require the collection of plasma or plasma and urine, respectively. Other drawbacks of radioactive labeled inulin include usage of isotopes, time consuming surgical preparation of the animals, and the requirement of a terminal experiment. Here we describe a method which uses a single bolus injection of fluorescein isothiocyanate-(FITC) labeled inulin and the measurement of its fluorescence in 1-2 μl of diluted plasma. By applying a two-compartment model, with 8 blood collections per mouse, it is possible to measure GFR in up to 24 mice per day using a special work-flow protocol. This method only requires brief isoflurane anesthesia with all the blood samples being collected in a non-restrained and awake mouse. Another advantage is that it is possible to follow mice over a period of several months and treatments (i.e. doing paired experiments with dietary changes or drug applications). We hope that this technique of measuring GFR is useful to other investigators studying mouse kidney function and will replace less accurate methods of estimating kidney function, such as plasma creatinine and blood urea nitrogen. 相似文献
15.
The ability of Climacodon septentrionalis to immobilize and kill a mycophagous nematode (Aphelenchoides sp.) in vitro is described for the first time. Two isolates produced droplets (20–45 μm in diameter) that formed at the apices of tall, stalked, and branching secretory cells (700–1,500 μm tall). On 2% modified malt extract agar, nematodes became enveloped in the droplets, which restricted their ability to move and resulted in complete immobilization and death within several hours of contact. The rate of decomposition of the nematodes varied considerably, with most individuals persisting for weeks whereas others were degraded within several days and appeared to be colonized by dense hyphal growth. This study provides the first documentation of a non-agaricoid fungus producing secretory cells that are able to immobilize nematodes. 相似文献
16.
Background and Aims: Myrmecophytes, or ant-plants, are characterized by their abilityto shelter colonies of some ant species in hollow structures,or ant-domatia, that are often formed by hypertrophy of theinternal tissue at specific locations (i.e. trunk, branches,thorns and leaf pouches). In Hirtella physophora (Chrysobalanaceae),the focal species of this study, the ant-domatia consist ofleaf pouches formed when the leaf rolls over onto itself tocreate two spheres at the base of the blade. Methods: The morphological and anatomical changes through which foliarant-domatia developed from the laminas are studied for the firsttime by using fresh and fixed mature leaves from the same H.physophora individuals. Key results: Ant-domatia were characterized by larger extra-floral nectaries,longer stomatal apertures and lower stomatal density. The anatomicalstructure of the domatia differed in the parenchymatous tissuewhere palisade and spongy parenchyma were indistinct; chloroplastdensity was lower and lignified sclerenchymal fibres were morenumerous compared with the lamina. In addition, the domatiawere thicker than the lamina, largely because the parenchymatousand epidermal cells were enlarged. Conclusions: Herein, the morphological and anatomical changes that permitfoliar ant-domatia to be defined as a specialized leaf structureare highlighted. Similarities as well as structural modificationsin the foliar ant-domatia compared with the lamina are discussedfrom botanical, functional and mutualistic points of view. Theseresults are also important to understanding the reciprocal evolutionarychanges in traits and, thus, the coevolutionary processes occurringin insect–plant mutualisms. 相似文献
17.
Previous research showed that addition of nutrient nitrogen to ligninolytic (stationary, nitrogen-starved) cultures of the wood-decomposing basidiomycete Phanerochaete chrysosporium causes a suppression of lignin degradation. The present study examined early effects on nitrogen metabolism that followed addition of NH
4
+
and l-glutamate at concentrations that yield similar patterns of suppression. Both nitrogenous compounds were rapidly assimilated (>80% in 6 h). Both caused an initial 80% or greater increase in the intracellular glutamate pool and had similar effects in increasing the specific activities of NADP- and NAD-glutamate dehydrogenases and glutamine synthetase. Differences between the effects of added NH
4
+
and glutamate showed that suppression was not correlated with intracellular pools of arginine or glutamine, nor was the maintenance of an elevated glutamate pool required to maintain the suppressed state. While a portion of the initial glutamate suppression could be attributed to an effect on central carbon metabolism through glutamate catabolism by NAD-glutamate dehydrogenase, the long term suppression by glutamate and the suppression by NH
4
+
were more specific. Suppression by NH
4
+
or glutamate in the presence or absence of protein synthesis (cycloheximide) followed essentially identical kinetics during 12 h. These results indicate that nitrogen additions cause a biochemical repression of enzymes associated with lignin degradation. Results are consistent with the hypothesis that nitrogen metabolism via glutamate plays a role in initiation of repression.Non-Standard Abbreviations DMS
2,2-dimethylsuccinate
- TCA
trichloroacetic acid 相似文献
18.
The tube systems known to exist in tracheophytes are xylem, phloem and laticifers which are composed of living or non-living cells and secretory ducts/canals and aerenchyma that is of schizogenous or lysigenous origin. Here, we describe a novel type of tube network of unknown function that ramifies through the olive tree bark and is composed of groups of apparently empty, anastomosing tubules interrupted by perforated plates; side openings of the tubules connect them to the intercellular spaces of the cortex. 相似文献
19.
目的:通过测量颧根与侧颅底各重要结构间的距离关系,为临床侧颅底外科手术治疗提供定位参考。方法:取成人颅骨标本50例(去颅盖标本8例,整颅42例)100侧,用游标卡尺、圆规和直尺测量颧根与侧颅底重要结构的距离。结果:实验测得左右侧颧根与外耳门前缘中点、乳突尖、茎突、翼突外侧板根部、舌下神经管外口、茎乳孔、颈静脉孔外缘、颈动脉管外口后缘、棘孔、卵圆孔、破裂孔的距离分别为22.30±2.84mm和22.02±3.27mm、40.37±3.21mm和40.56±3.54mm、32.53±2.78mm和32.92±2.68mm、35.13±3.14mm和35.19±2.74mm、49.29±2.88mm和48.98±2.87mm、32.92±2.44mm和33.05±2.61mm、35.15±2.86mm和34.68±3.13mm、33.17±2.78mm和33.17±2.72mm、28.83±2.62mm和28.68±2.63mm、31.15±2.76mm和31.49±2.73mm、43.67±3.32mm和44.15±3.02mm,左右侧数据无差异(P〉0.05)。结论:颧根可以作为侧颅底外科手术的定位标志,为直视条件下经颞下等入路的侧颅底外科手术提供解剖学依据。 相似文献
20.
Nhu H. Nguyen Sung-Oui Suh Christopher J. Marshall Meredith Blackwell 《Mycological Research》2006,110(10):1232-1241
Ascomycete yeasts that both ferment and assimilate xylose were reported previously as associates of insects living in woody substrates. Most notable have been reports of Pichia stipitis-like yeasts that are widely associated with the wood-boring beetle, Odontotaenius disjunctus (Coleoptera: Passalidae), in the eastern United States. Our continuing investigation of insect gut yeasts has lead to the discovery of two new xylose-fermenting yeasts that phylogenetic analysis places as sister taxa. The beetle hosts, O. disjunctus and Phrenapates bennetti (Coleoptera: Tenebrionidae), are similar in habitat and appearance, and the presence of similar gut yeasts is an additional common feature between them. Here we describe the new yeast genus Spathaspora, the type species S. passalidarum, and its sister taxon Candida jeffriesii and discuss their natural history, including a comparison with Pichia stipitis, another member of a guild of xylose-fermenting yeasts with similar metabolic traits. In addition a morphologically distinct yeast ascospore type is described for Spathaspora. 相似文献