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91.
The outline of the adult wing of lepidopteran insects (butterflies and moths) emerges as a result of disappearance of a group of cells at the periphery of the pupal wing. Histological observation of the pupal wing of Pieris rapae showed that, just after apolysis of the wing epithelium from the pupal cuticle, there occurs a rapid and localized decrease of the number of cells at the periphery of the wing. This decrease occurs through cell death, which lasts 1–1.5 days at 20°C. Dying cells lose contact with the neighbouring cells and show condensation of chromatin and cytoplasm. They then appear to be phagocytosed by neighbouring epithelial cells or discharged through the basal surface of the epithelium into the lumen within the wing and taken up by phagocytes. Fragmentation of DNA in the nuclei was detected in the dead cells or their debris. These results indicate that programmed cell death in the lepidopteran wing proceeds through a mechanism closely similar to that of apoptosis in the vertebrate. 相似文献
92.
Stephen M. Ferkovich Stephen G. Miller Herbert Oberlander 《Archives of insect biochemistry and physiology》1995,29(4):381-390
An N-terminal amino acid sequence of a 16.9 kDa hemolymph polypeptide, “Vesicle Promoting Factor” (VPF) from Trichoplusia ni, revealed a high sequence homology (70%) with Manduca sexta apolipophorin-III. A polyclonal antibody developed against VPF, however, was not immunoreactive with either purified M. sexta or T. ni apolipophorin-III. Immunoblots of tissue homogenates of T. ni indicated that VPF was present in imaginal wing discs, central nervous system (CNS), silk glands, midgut and hemocytes from fifth instar larvae, and also in the IAL-TND1 cell line which can grow as either fluid-filled multicellular vesicles or multicellular aggregates. VPF was also detected immunologically in the hemolymph of adults of T. ni, and in hemolymph of adults and larvae of Galleria mellonella and Heliothis virescens. Testes, midgut, hemocytes, and wing discs, but not Malpighian tubules, of T. ni released VPF into tissue culture medium during a 3 h incubation period. © 1995 Wiley-Liss, Inc. 1 This article is a US Government work and, as such, is in the public domain in the United States of America. 相似文献
93.
Piotr Mikoajczyk Grazyna Zimowska Herbert Oberlander Donald L. Silhacek 《Archives of insect biochemistry and physiology》1995,28(2):173-187
We determined the contribution of the peripodial membrane to chitin synthesis in cultured wing imaginal discs of Spodoptera frugiperda. This was accomplished by examining chitin synthesis in vitro in intact imaginal discs, in the peripodial membrane, and in imaginal discs in which the peripodial membrane had been injured. Chitin synthesis in peripodial membrane-deprived imaginal discs, peripodial membrane injured imaginal discs, and peripodial membrane fragments was assessed by measuring incorporation of [14C]GlcNAc after treatment with 20-hydroxyecdysone in tissue culture. Removing or injuring the peripodial membrane resulted in a marked decrease in ecdysteroid-dependent chitin synthesis in these wing discs compared with intact wing discs. In addition, a break in the ecdysteroid treatment of 4 h reduced chitin synthesis in the wing discs substantially. These biochemical experiments were supplemented with ultrastructural and immunocytochemical approaches. A wheat germ agglutinin colloidal gold complex was used to visualize the presence of chitin synthesized by wing discs including the peripodial membrane. These experiments confirmed the importance of an intact peripodial membrane for optimal production of cuticle by the wing pouch. Our results demonstrate that for opti-ma1 production of chitin in tissue culture, wing discs must be treated with 20-hydroxyecdysone for an uninterrupted period of 48 h, and the peripodial membrane of these imaginal discs must be present and uninjured. © 1995 Wiley-Liss, Inc. 1 This article is a US Government work and, as such, is in the public domain in the United States of America. 相似文献
94.
Abstract. Sesamia nonagrioides Lefebvre diapauses as mature larvae. Once diapause is induced, larvae continue to grow and moult with no apparent changes, so distinction between non-diapausing and diapausing larvae is difficult. In the present work two physiological markers of diapause induction are obtained and their efficacy in detecting diapause onset and in monitoring diapause development in field populations is evaluated. The first marker is based on the differential capacity of two photoregimes, LD 0:24 h and LD 16:8 h, to reverse diapause induction. When larvae of the first and second stadia were subjected to the diapause-inducing LD 12:12 h light cycle and then transferred to LD 0:24 h, larvae pupated after a development duration significantly higher than that of the larvae transferred to LD 168 h. This criterion was used to monitor diapause development in overwintering larvae until field-collected larvae submitted to LD 0:24 h pupated synchronously with those submitted to LD 16:8 h. This occurred between late January and early March. The second marker refers to development of imaginal wing discs. A relative index of the tracheal mass area divided by the total disc area diminishes steadily during development in non-diapausing sixth-instar larvae until it becomes zero just before pupation, whereas it remains constant in diapausing larvae. Though wing disc development was blocked in diapausing sixth-instar larvae, the disc continued to grow and, consequently, the total area of the disc was not a suitable marker of diapause induction. When the constancy of the relative area index was used to monitor diapause development and termination in overwintering larvae, the results agreed with those obtained using the first criterion and with previous field observations. 相似文献
95.
Seasonal changes in the percentage of macropterous individuals in natural population of Microvelia douglasi in permanent habitats were investigated in Shimane, western part of Japan. Macropterous forms of 5th instar nymphs occurred most frequently from late June to early July. However, the seasonal appearance of macropterous adults differed between habitats and years. There were significant effects of density, temperature, photoperiod and food availability on the percentage of macropters under laboratory conditions. The percentage of macropterous forms generally increased with density, but the mode of the response was modified by temperature, photoperiod and food availability. When insects were reared under long day photperiod (16L8D) with abundant food (0.5–1.0 g/container/day), the percentage of macroptery varied most among the different density treatments at the intermediate temperature (24°C) and the difference was reduced at higher temperature (28° and 32°C). Effects of temperature were small under the short day photoperiod (12L12D). Under 12L12D photoperiod sensitivity of density responses were higher at 32°C and lower at 24°C than those under 15L8D photoperiod. When the insects were supplied with food every other day, the density response became obscure. Ecological significance of environmental responses in the wing morph determination was discussed in relation to the heterogeneous environment of water surface. 相似文献
96.
Konjev Desender 《Oecologia》1989,78(4):513-520
Summary The wing-polymorphic ground beetle Pogonus chalceus MARSHAM was subjected to crossbreeding experiments under different laboratory conditions in order to estimate the genetic and environmental contributions to the total phenotypic variance in different morphological traits related to relative wing development and body size. Heritability of relative wing development appears to be strong. Beetle size also seems genetically determined in some cases, but separation of male and female parent contribution invariably shows a maternal effect. These results are tested in a breeding experiment with a high number of progeny from one parental pair, reared at two temperatures and at two levels of food supply. Relative wing development is not influenced by these environmental conditions, as expected, but different temperatures add significant variance to the body size values. The experimental results are used to explain interdemic variation in these morphological traits, as observed in three isolated field populations. The reproductive effort under optimum breeding conditions is higher in macropterous beetles than in beetles with reduced wings, but this could result from their larger body size. Migtion seems to be the most plausible underlying evolutionary mechanism for the observed wing reduction in older populations. 相似文献
97.
A simple and rapid method for the isolation of intercalated discs from a single rat heart is described. The outstanding features of this method are (i) a large quantity of starting material is not necessary, (ii) ultracentrifugation and separating gradient steps are not required, (iii) the disc fraction is relatively pure as verified by electron microscopy, and (iv) the starting tissue is rapidly homogenized into buffer, reducing possible damage to membrane structures after animal death. The fraction is rich in intercellular junctions, with gap junctions, fasciae adherentes and maculae adherentes all appearing well preserved. It should prove a useful starting base for further purification of these junctions and of sarcolemma from a specific portion of the cell surface membrane, namely the major area of structural interaction between adjacent cardiac cells. 相似文献
98.
Minoru Murai 《Population Ecology》1976,18(1):147-159
Summary Two adjacent stations (H and L) were set up to study movement ofC. saccharivorus adults in sugarcane fields. At the beginning of the study the density ofC. saccharivorus including all stages of development was quite different between the two. The density of the first generation adult on Station
H was about 5 times that on Station L. The number ofC. saccharivorus on both the stations became almost the same one month after the beginning of the study. At the beginning of the study macropterous
adults were more numerous in Station H than in Station L. However percentage of macropterous adults on Station L increased
after one month whereas that on Station H declined.
About 2,000 marked adults were released on each station during the early period of the emergence of the first generation adult.
Marked insects were recaptured on both the stations one month after the release. The adults released on the dense population
(H) tended to disperse more actively than those on the scarce population (L). Marked macropterous adults moved more actively
than brachypterous ones. The density related dispersal of adults was considered to be an important factor to regulate the
population density. 相似文献
99.
Summary The tropical earwigs Paralabella dorsalis and Pseudomarava prominensis are polymorphic for wings in both sexes. An individual's chances of being winged are influenced by whether its parents were winged, by its own sex, and by its nymphal nutrition (at least in P. dorsalis). Wingless females of P. dorsalis lived longer, started ovipositing ealier, and laid slightly larger clutches. 相似文献
100.
Frank Y. T. Sin 《Molecular biology reports》1987,12(1):7-11
The insect moulting hormone, 20-hydroxyecdysone, induces the synthesis of two groups of proteins in the imaginal wing discs ofDrosophila melanogaster. The early induced group appears about 4 h after hormone treatment, the late induced group appears about 12 h after treatment. Studies using -amanitin to inhibit mRNA synthesis during the period of hormonal treatment showed that the induction of the late proteins is dependent on mRNA synthesis and possibly the synthesis of the early proteins. 相似文献