RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species

Summary RFLP variability was studied in eight U.S. peanut cultivars, representing the four market types, and in 14 wild Arachis species accessions, using random genomic clones from a PstI library. Very low levels of RFLP variability were found among the allotetraploids, which included the U.S. cultivars and Arachis monticola, a wild species. The diploid wild species were very diverse, however. RFLP patterns of the allotetraploids were more complex than the diploids, and the two constituent genomes could usually be distinguished. On the basis of RFLP band sharing, A. ipaensis, A. duranensis, and A. spegazzinii appeared most closely related to the diploid progenitor species of the allotetraploids. A dendrogram of relationships among the diploid wild species was constructed based on band sharing.     

The dielectric permittivity at radio frequencies and the bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell cultures

A novel technique is described for the measurement of the volume fraction of biomass in a suspension by the simultaneous measurement of the conductivity of a suspension containing cells and of the medium in which the cells are suspended. The presence of non-conducting particulate matter in a suspension will cause the conductivity of a suspension to be decreased relative to that of the medium in which the particles are suspended. A simple equation (the Bruggeman equation) describes the relationship between the volume fraction of non-conducting particulate matter and the decrease in conductivity. The accuracy of this method for the determination of the biomass concentration of plant cells (Festuca arundinacea) in culture was shown. The method was successfully applied to the on-line determination of biomass concentrations during the growth of F. arundinacea cultures, and gave good agreement with biomass levels as determined from measurements of the radio-frequency dielectric permittivity of such cultures.     

荧光显微术在当代植物细胞生物学研究中的应用

自从八十年前第一次在显微镜下观察到生物组织经紫外线照射后发射荧光的现象以来,荧光显微术不断获得进步,现已发展成细胞生物学中一个重要的研究手段。高度的灵敏性和专一性、制样与观察程序的简便、尤其是适宜于活细胞研究等特点,是它所具有的独特长处。荧光显微术特别是免疫荧光技术在现代医学生物学研究中的应用是一个     

EUF-nutrient contents required for optimal nutrition of grapes

Summary Our field surveys conducted in 10 Hungarian grape producing areas have revealed that the nutrient contents of vine leaves of different grape varieties were closely correlated with the EUF-nutrient contents of different soil types with different contents of clay. Resulting from these relationships the following EUF-nutrient values are considered as required for optimal nutrition of the vine-stock to attain grape yields of 10–12 t/ha in the areas under investigation:     

Comparison of rates of natural senescence of the root cortex of wheat (with and without mildew infection), barley,oats and rye

Summary In comparative tests in a glasshouse, the cortex of oat and rye roots senesced more slowly than the cortex of wheat and barley roots. Of the cereals tested, wheat showed the most rapid rate of root cortical senescence, and the rate was unaffected by inoculation of leaves withErysiphe graminis. The results are discussed in relation to infection by root pathogens.     

Genotype dependent variation in mycorrhizal colonization and response to inoculation of pearl millet

Summary Genotypes of pearl millet (Pennisetum americanum L. Leeke) were examined for differences in vesicular-arbuscular mycorrhizal (VAM) colonization and response to inoculation. For thirty genotypes tested across three field locations there was a range of mycorrhizal colonization intensity between 25 and 56%. In another experiment with two male-sterile lines, restorer lines and their derived crosses, grown in pots filled with non-sterilized soil there were significant differences between genotypes for colonization by mycorrhiza. This showed hostgenotype dependence for mycorrhizal colonization.Root growth rates, mycorrhizal root length, percentage root colonization and plant growth and P uptake were studied in ten genotypes. A set of 3 genotypes with similar root lengths varied significantly with regard to mycorrhizal root length and the percentage colonization. This supports the suggestion that VAM colonization and spread is dependent on the host genotype. The growth responses differed significantly between the genotypes and they also differed in their responses to P uptake and VAM inoculation. The utility of host-genotype dependent differences in VAM symbiosis in plant breeding is discussed.Journal Article No. 453     

Optimum utilization of sewage sludge of low and high metal content for grain production on arid lands

Summary Two municipal sludges, one from a highly industrialized city, Chicago, Il, and another from a little industrialized, highly agricultural area, Tucson, AZ are compared for winter barley production on Pima c 1 (Typic torrifluvent). Both sludges were responsible for highly significant additions of Zn, Cu, Ni, Cd and P to the soil each year when applied at the rates of 100 mt/ha singly and 20 mt/ha each year for 4 years. Nitrogen responses for barley straw and grain were observed from both sludges. Tucson sludge appears to be attractive as a potential fertilizer, not only as an NPK source, but also for its organic matter and minimal amounts of heavy metals. The Chicago sludge with relatively high levels of heavy metals, particularly Cd, appears poorly suited as a fertilizer, if used for an extended period of time, because of the plant's tendency to take up elevated levels of certain heavy metals. Some parts of barley plants proved to be a better indicator of heavy metal uptake and concentration than others. The diagnostic-tissue test promises to be a useful tool to warn against undesirable accumulation of heavy metals. Fortunately, when compared with other plant parts, the heavy metal in grain was the least altered as a result of continued sewage sludge use on arid land. The soil's neutral to slightly alkaline pH and the presence of lime throughout the soil profile appeared to be critical factors in keeping plant uptake of heavy metals relatively low as compared with soils of other climates.     

Some aspects of the natural history and food selection ofAvahi laniger

Two groups ofAvahi laniger were studied in the Forêt de Analamozoatra near Perinet in the eastern rainforest of Madagascar from August to October 1984. Overlap between the home ranges of neighbouring groups ofA. laniger was minimal. Group size ranged from one to four individuals with a median group size of two. In four out of ten groups a baby was born between August and September.A. laniger were most active after dusk and before dawn. They had an extended resting period around midnight. Their diet consisted mostly of leaves from at least 17 different plant species. They also ate flowers. Fruit eating was recorded twice. Leaves eaten had high contents of protein and sugar but did not contain alkaloids. The concentration of condensed tannins did not differ between food items and non-food items. There was no indication of competition with other prosimians that might explain their nocturnality.     

Regeneration of peach plants from callus derived from immature embryos

Summary Peach plants were repeatedly regenerated from immature embryos but not from callus derived from mature embryos. A white, nodular, highly regenerative callus was obtained when friable, primary callus from immature embryos was transferred from medium containing 4.5 M 2,4-dichlorophenoxyacetic acid and 0.44 M benzyladenine (BA) to media containing 0.27 M -naphthaleneacetic acid (NAA) and 2.2 M BA. This callus retained its morphogenetic potential for a minimum of three subcultures. Green nodular callus, that lacked regenerative capacity, was produced from primary callus derived from mature embryos. Maximum regeneration of shoots occurred when highly regenerative callus was transferred to a medium in which the NAA concentration was reduced five times and the BA concentration was increased two times. Regenerated shoots were rooted in the dark on a medium containing 28.5 M indoleacetic acid. Cytogenetic analysis of regenerated plants indicated that all plants were diploid, 2n = 2x = 16. Phenotypic evaluation of regenerated plants, grown under field conditions, is now in progress.     

Genetical analysis of chromosome 5A of wheat and its influence on important agronomic characters

Summary Chromosome 5A of bread wheat, Triticum aestivum carries the major gene, Vrnl, which is one of the main determinants of the winter/spring growth habit polymorphism in this species. Genetical analysis of this chromosome has been carried out using single-chromosome recombinant lines to establish the pleiotropic effects of this locus and two other major genes, q determining ear morphology and bl determining the presence of awns, on important agronomic characters. The three major genes were located on the long arm of chromosome 5A with a gene order of: centromere -bl-q-Vrnl. Analysis of quantitative characters from a winter sowing revealed pleiotropic effects of Vrnl or the effects of closely linked loci on the characters plant height, tiller number and spikelet number. However effects on ear emergence time were not associated with Vrnl but with q as were effects on spikelet number and ear length. In addition a locus determining yield/plant was located between Vrnl and q. Independant loci determining height and ear length were apparent on the short arm of chromosome 5A. From a spring sowing, however, there was a large pleiotropic effect of Vrnl on ear emergence time, as well as the effects previously detected. In addition, associated with q were effects on plant height and grain size which were not expressed from the winter sowing.