Predict prokaryotic proteins through detecting N-formylmethionine residues in protein sequences using support vector machine

Identifying prokaryotes in silico is commonly based on DNA sequences. In experiments where DNA sequences may not be immediately available, we need to have a different approach to detect prokaryotes based on RNA or protein sequences. N-formylmethionine (fMet) is known as a typical characteristic of prokaryotes. A web tool has been implemented here for predicting prokaryotes through detecting the N-formylmethionine residues in protein sequences. The predictor is constructed using support vector machine. An online predictor has been implemented using Python. The implemented predictor is able to achieve the total prediction accuracy 80% with the specificity 80% and the sensitivity 81%.     

Web-based telemicroscopy

By taking advantage of network-based computing and the recent developments in Web interfaces, centralized research facilities housing specialized and unique imaging instruments along with associated high-performance computing can be made available to researchers for use from their own laboratories. In addition to increasing access and utilization of these facilities, operation over the Internet is expected to enhance research by facilitating collaboration between researchers. We describe the implementation of a platform-independent Web-based system written in Java that supplements automated functions with video-guided interactive, collaborative remote control and data acquisition from an intermediate-high-voltage electron microscope.     

上海昆虫多样性信息系统的设计与建立

利用Access＋ASP＋IIs技术,建立了集害虫信息管理与昆虫编目数据库为一体的上海昆虫多样性管理信息系统。用户可以通过浏览器访问http：／／www．insects．cn进行检索和查询。本文详细阐述了该系统的构建方法,并就目前昆虫多样性信息管理系统建设中存在的问题作了评述。     

三江源区生态系统和土壤保持服务对未来气候变化的响应特征

气候变化条件下的生态系统响应特征对生态系统服务的提升和生态环境保护具有重要意义。现有气候变化评估多以全球或区域大尺度研究为主,不适合局地小尺度。以2015年为基准,根据局地特征改进了综合顺序分类系统（CSCS）,模拟了未来不同温室气体排放模式下三江源地区自然植被分布,同时分析了植被覆盖度及土壤保持功能的时空变化。研究结果表明：①在2080年的不同排放情境下,三江源地区的降水和气温增长主要发生在生长季（5—9月）和半湿润地区,其中,高排放情境的增幅最大（42.21 mm和4.93℃）;②三江源地区潜在自然植被主要由草地转化为森林,植被覆盖度和土壤保持服务在不同排放情境下均呈现由西北向东南增加的趋势,与水热规律一致,其中高排放情境的增幅最大,低排放最小;③ 利用三江源地区原生裸地演替特点和多年NPP改进的CSCS模型,模拟精度提高了24%。研究成果为局地小尺度生态系统气候响应特征提供了必要的手段和规律认识。     

A survey of the availability of primary bioinformatics web resources

The explosive growth of the bioinformatics field has led to a large amount of data and software applications publicly available as web resources. However, the lack of persistence of web references is a barrier to a comprehensive shared access. We conducted a study of the current availability and other features of primary bioinforo matics web resources （such as software tools and databases）. The majority （95%） of the examined bioinformatics web resources were found running on UNIX/Linux operating systems, and the most widely used web server was found to be Apache （or Apache-related products）. Of the overall 1,130 Uniform Resource Locators （URLs） examined, 91% were highly available （more than 90% of the time）, while only 4% showed low accessibility （less than 50% of the time） during the survey. Furthermore, the most common URL failure modes are presented and analyzed.     

基于非核糖体肽的文献计量分析

许多临床上的重要抗生素来源于微生物生产的非核糖体肽类天然产物或者聚酮-非核糖体肽杂合体类天然产物,本文选取了近5年Web of Science上关于非核糖体肽的国际期刊文献,采用文献计量、统计分析等方法展示非核糖体肽研究领域的热点方向,探究了该领域的发展趋势,以期为进一步研究提供参考。     

Spatiotemporal Clusters and Trends of Pneumocystis Pneumonia in Korea

This study determined the recent status and trend of Pneumocystis jirovecii pneumonia (PcP) in the non-human immunodeficiency virus (HIV) (non-HIV-PcP) and HIV (HIV-PcP) infected populations using data from the Health Insurance Review & Assessment Service (HIRA) and the Korea Disease Control and Prevention Agency (KDCA). SaTScan and Joinpoint were used for statistical analyses. Non-HIV-PcP cases showed an upward trend during the study period from 2010 to 2021, with the largest number in 2021 (551 cases). The upward trend was similar until 2020 after adjusting for the population. Seoul had the highest number of cases (1,597) in the non-HIV-PcP group, which was the same after adjusting for the population (162 cases/1,000,000). It was followed by Jeju-do (89 cases/1,000,000). The most likely cluster (MLC) for the non-HIV-PCP group was Seoul (Relative Risk (RR)=4.59, Log Likelihood Ratio (LLR)=825.531), followed by Jeju-do (RR=1.59, LLR=5.431). An upward trend was observed among the non-HIV-PcP group in the Jeju-do/Jeollanam-do/Jeollabuk-do/Gyeongsangnam-do/Busan/Daejeon/Daegu/Ulsan joint cluster (29.02%, LLR=11.638, P<0.001) located in the southern part of Korea. Both women and men in the non-HIV groups showed an overall upward trend of PcP during the study period. Men in the 60–69 age group had the highest annual percentage change (APC 41.8) during 2014–2019. In contrast, the HIV groups showed a falling trend of PcP recently. Men in the 60–69 age group had the most decrease (APC −17.6) during 2018–2021. This study provides an analytic basis for health measures and a nationwide epidemiological surveillance system for the management of PcP.     

VitisMod:一个葡萄基因共表达数据库

葡萄转录组已在各种组织、发育阶段、生物胁迫、非生物胁迫和其他条件下被测定。目前,仍没有简单实用的网络工具来探索这些宝贵的数据。本文从美国国立生物技术信息中心的基因表达数据库(NCBI GEO)下载1019个基因表达芯片数据,进行权重基因共表达网络分析(WGCNA)。鉴定到41个基因共表达模块。功能富集分析发现这些模块具有不同的功能,并与实验/表型相关。通过模块内连接度筛选枢纽基因,这些基因可能具有重要功能。通过关联推定(Guilt-by-association)原理对模块内功能未知的基因进行功能预测。最后,构建了免费的网络工具VitisMod,为葡萄的基因功能研究提供新资源,网址为：http://bioinformatics.fafu.edu.cn/grape。     

在住院处深入开展以人为本与和谐共存服务理念的探讨

目的：为构建和谐的医患关系,为病人提供优质服务,结合住院处的实际情况,探讨在医院住院处深入开展以人为本与和谐共存服务理念的重要性和必要性。方法：详细介绍以人为本与和谐共存服务理念,简要概括医院住院处的工作职责及其在医院系统中的作用和地位,将以人为本与和谐共存服务理念与医院住院处的职责相结合,探讨该理念在提升住院处的工作职能、促进与患者的沟通交流、提升医院信誉、维护医院形象中的作用。结果：以人为本与和谐共存服务理念在指导住院处各项工作中确实有一定的引导作用。结论：将以人为本与和谐共存服务理念在提升住院处的工作职能相结合,可以很好地促进医务人员与患者的沟通交流,提升医院信誉,提高医院竞争力。