Replication study implicates COMT val158met polymorphism as a modulator of probabilistic reward learning

Previous studies suggest that a single nucleotide polymorphism in the catechol‐O‐methyltransferase (COMT) gene (val158met) may modulate reward‐guided decision making in healthy individuals. The polymorphism affects dopamine catabolism and thus modulates prefrontal dopamine levels, which may lead to variation in individual responses to risk and reward. We previously showed, using tasks that index reward responsiveness (measured by responses bias towards reinforced stimuli) and risk taking (measured by the Balloon Analogue Risk Task), that COMT met homozygotes had increased reward responsiveness and, thus, an increased propensity to seek reward. In this study, we sought to replicate these effects in a larger, independent cohort of Caucasian UK university students and staff with similar demographic characteristics (n = 101; 54 females, mean age: 22.2 years). Similarly to our previous study, we observed a significant trial × COMT genotype interaction (P = 0.047; η² = 0.052), which was driven by a significant effect of COMT on the incremental acquisition of response bias [response bias at block 3 ? block 1 (met/met > val/val: P = 0.028) and block 3 ? block 2 (met/met > val/val: P = 0.007)], suggesting that COMT met homozygotes demonstrated higher levels of reward responsiveness by the end of the task. However, we failed to see main effects of COMT genotype on overall response bias or risk‐seeking behaviour. These results provide additional evidence that prefrontal dopaminergic variation may have a role in reward responsiveness, but not risk‐seeking behaviour. Our findings may have implications for neuropsychiatric disorders characterized by clinical deficits in reward processing such as anhedonia.     

pH responsiveness of fibrous assemblies of repeat-sequence amphipathic α-helix polypeptides

We reported previously that our designed polypeptide α3 (21 residues), which has three repeats of a seven-amino-acid sequence (LETLAKA)₃, forms not only an amphipathic α-helix structure but also long fibrous assemblies in aqueous solution. To address the relationship between the electrical states of the polypeptide and its α-helix and fibrous assembly formation, we characterized mutated polypeptides in which charged amino acid residues of α3 were replaced with Ser. We prepared the following polypeptides: 2Sα3 (LSTLAKA)₃, in which all Glu residues were replaced with Ser residues; 6Sα3 (LETLASA)₃, in which all Lys residues were replaced with Ser; and 2S6Sα3 (LSTLASA)₃; in which all Glu and Lys residues were replaced with Ser. In 0.1M KCl, 2Sα3 formed an α-helix under basic conditions and 6Sα3 formed an α-helix under acid conditions. In 1M KCl, they both formed α-helices under a wide pH range. In addition, 2Sα3 and 6Sα3 formed fibrous assemblies under the same buffer conditions in which they formed α-helices. α-Helix and fibrous assembly formation by these polypeptides was reversible in a pH-dependent manner. In contrast, 2S6Sα3 formed an α-helix under basic conditions in 1M KCl. Taken together, these findings reveal that the charge states of the charged amino acid residues and the charge state of the Leu residue located at the terminus play an important role in α-helix formation.     

Characterization of isogenic carp (Cyprinus carpio L.) lines with a genetically determined high or low antibody production

Antibody production to dinitrophenyl-keyhole limpet haemocyanin (DNP-KLH) served as the immune parameter to divergently select carp (Cyprinus carpio L.) to produce high- and low-responder F, hybrid lines. Antibody production to trinitrophenyl-lipopolysaccharide [TNP-LPS) and to DNP-KLH were similar in magnitude. By contrast, some high-responder lines were low responders to DNP-human serum albumin, and vice versa. Low-responder carp were relatively susceptible to infection with the parasite Trypanoplasma borreli. This suggested that at least one gene with a major influence on resistance differed between the two homozygous parents (69, 85) used to generate the high- and low-responder homozygous families, respectively. The isogenic lines showed no within-line variation in DNA fingerprints, but differed with respect to their MhcCyca-DAB genes.     

SPECT体积测试中系统误差校正初探

本文详细的介绍了一种在用ＳＰＥＣＴ测量脏器体积中校正系统误差的有效方法。对动物试验的结果,相对误差小于５％,证明了这一方法的可靠性和实用意义。文章还对测试结果和这一方法的改进作了进一步的讨论。     

Identification of QTLs influencing wood property traits in loblolly pine (Pinus taeda L.). I. Physical wood properties

Physical wood property traits for loblolly pine (Pinus taeda L.) were analyzed for the presence of quantitative trait loci (QTLs) in a three-generation outbred pedigree. These traits include wood specific gravity (wsg), volume percentage of latewood (vol%), and microfibril angle (mfa). Phenotypic data were collected for rings 2–11 for wsg and vol%, and rings 3, 5 and 7 for mfa. Both earlywood and latewood were analyzed for each trait. An interval mapping approach designed for an outbred pedigree was used to estimate the number of QTLs, the magnitude of QTL effects, and their genomic position. Nine unique QTLs were detected for composite traits (average of all rings) for wsg, five for vol%, and five for mfa. The majority of these QTLs were verified by analyses of individual-ring traits. Additional QTLs for each trait were also detected by these individual-ring analyses. Most QTLs for wsg were specific to either earlywood or latewood, whereas each QTL for mfa was detected for both earlywood and latewood. Before these QTLs are utilized in a breeding program, they should be verified in larger experiments and in different genetic and environmental backgrounds. Received: 15 February 2000 / Accepted: 31 March 2000     

Specific action of blue light on phytochrome-mediated enzyme syntheses in the shoot of milo (Sorghum vulgare Pers.)

Abstract. This paper describes the effect of prolonged treatments with red or blue light on the capacity of the milo ( Sorghum vulgare Pers.) shoot to respond to Pfr in subsequent darkness. Two groups of enzymes were studied. In group I (NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, NADP-GPD. EC 1.2.1.13 and ribulose-bisphosphate carboxylase, carboxylase, EC 4.1.1.39) enzyme formation is strongly enhanced by red light pulses (operating through phytochrome) whereas in group II (NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD-GPD, EC 1.2.1.12 and NAD-dependent malate dehydrogenase, MDH. EC 1.1.1.37) enzyme formation hardly responds to red light pulses.
In group 1 a 24-h treatment with blue light (but not with red light) leads to a strong increase in responsivity to Pfr whereas in group II a 24-h treatment with blue or red light does not increase responsivity to Pfr in subsequent darkness.
The specific effect of blue light cannot be explained by an effect of light on gross protein synthesis. Rather, the data indicate that amplification of responsivity to Pfr by blue light is a specific process directly related to the mechanism of modulation of gene expression by phytochrome.     

Volume restoration in osmotically swollen lymphocytes does not involve changes in free Ca2+ concentration

An increase in cytoplasmic free [Ca²⁺], [Ca²⁺]_i, has been suggested as the trigger for the permeability changes that bring about cell volume restoration following exposure to anisotonic media. This idea was directly tested in human peripheral lymphocytes undergoing regulatory volume decrease following a hypotonic dilution of the suspension. [Ca²⁺]_i was measured with the intracellularly trapped fluorescent indicator, quin2, and showed no measurable change on hypotonic swelling or during the subsequent volume decrease. Moreover, even though the incorporated quin2 adds significant Ca-buffering to the cytoplasm, regulatory volume decrease occurred normally in the quin2-loaded cells. It appears that alterations in [Ca²⁺]_i are not involved in these processes of volume regulation. An intracellularly trapped derivative of fluorescein, bis(carboxyethyl)carboxyfluorescein, was used to monitor cytoplasmic pH, which also showed no change during regulatory volume decrease.     

Mobile charges in the cell membranes ofHalicystis parvula

Charge-pulse experiments were performed on cells of the giant marine algaHalicystis parvula. At normal pH (8.2), the voltage decay following a charge-pulse of 500 ns duration fed to the vacuole could be described by summing two exponential relaxations. The amplitudes and time constants of these relaxations were widely separated. The parameters of the two relaxation processes were found to be pH-dependent. Reduction of the external pH value from pH 8.2 to 5 resulted in a complete change of the two relaxation processes within a few minutes. Only one relaxation process could be observed at pH 5, within the time resolution of our instrumentation. The experimental data could not be explained by a two-membrane model with reasonable values for the specific capacitances of tonoplast and plasmalemma. The results of the charge-pulse relaxations were found to be consistent with the assumption that both membranes have very similar electrical properties and that both contain mobile charges with a total surface concentration of about 30 nmol·m^-2 and a translocation-rate constant of about 500·s^-1. The mobile charges became neutralized at pH 5 hhich led to a decrease of the apparent specific capacitance of the algal cells. They are presumably either part of a transport system for cations or connected with the chloride pump ofHalicystis parvula.Abbreviation RC (R)esistance·(C)apacitance     

Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture

Summary The genetics behind response in barley anther culture was studied with 22 reciprocal and one single: cross between three varieties with high and four varieties with low capacity for green plant formation. Effects of genotypes dominated embryo formation and percentages of green plants, accounting for 62 and 76% of total variation, respectively, with almost no genetic effect on the ability to regenerate plants from pollen embryos. Nuclear genes could explain all genotype effects in this plant material, since no reciprocal effects were indicated. The three parents with high and the four parents with low capacity for green plant formation formed two phenotypically homogeneous groups, producing 27–52% and 0–7% green plants, respectively. Genetic variation within hybrids for both embryo and green plant formation could be explained completely by general combining ability (GCA). The results are discussed with respect to a previous similar study in hexaploid wheat and the reported existence of DNA deletions in the plastid genomes in albino plants from anther culture of wheat and barley.     

Effect of chronic microwave radiation on T cell-mediated immunity in the rabbit

Experiments were conducted to elucidate the effects of chronic low power-level microwave radiation on the immunological systems of rabbits. Fourteen male Belgian white rabbits were exposed to microwave radiation at 5 mW/cm², 2.1 GHz, 3 h daily, 6 days/week for 3 months in two batches of 7 each in specially designed miniature anechoicchambers. Seven rabbits were subjected to sham exposure for identical duration. The microwave energy was provided through S band standard gain horns connected to a 4K3SJ2 Klystron power amplifier. The first batch of animals were assessed for T lymphocyte-mediated cellular immune response mechanisms and the second batch of animals for B lymphocyte-mediated humoral immune response mechanisms. The peripheral blood samples collected monthly during microwave/sham exposure and during follow-up (5/14 days after termination of exposures, in the second batch animals only) were analysed for T lymphocyte numbers and their mitogen responsiveness to ConA and PHA. Significant suppression of T lymphocyte numbers was noted in the microwave group at 2 months (P<0.01, % 21.5%) and during follow-up (P<0.01, % 30.2%). The first batch animals were initially sensitised with BCG and challenged with tuberculin (0.03 ml) at the termination of microwave irradiation/sham exposure and the increase in foot pad thickness ( mm), which is a measure of T cell-mediated immunity (delayed type hypersensitivity response, DTH) was noted in both the groups. The microwave group revealed a better response than the control group (%+12.4 vs.+7.54). The animals were sacrified and the tissue T lymphocyte counts (spleen and lymph node) were analysed. No significant variation was observed in the tissue T lymphocyte counts of microwave-irradiated rabbits. From these results it is speculated that the T lymphocytes are sequestered to various lymphoid organs under the influence of microwaves. A sub-population of T cells known as T helper cells (mediating DTH response) are probably not affected by microwave radiation. It is clear from our experiments that although chronic microwave radiation at 5 mW/cm² leads to suppression of peripheral T lymphocyte numbers, there is no concomitant functional impairment of these cells as evidenced by functional assays.