Papyrus swamp development in the Upemba Basin, Zaïre: studies of population structure in Cyperus papyrus stands

The ecology and distribution of papyrus swamps are briefly reviewed together with the ecology, distribution structure and growth characteristics of Cyperus papyrus.
Three age-classes of culm are recognized and girth and density of each are recorded along transects through mree swamps in the Upemba Basin of Zaïre. A regression of culm-unit biomass on culm girth, developed for Ugandan swamps, is used to convert Upemba measurements into biomasses. It is shown that simple measurements, taken at only a single point in time, yield much information on both the structure and the dynamics of papyrus populations.
It is suggested that the standing-crops of such populations are determined by available light, and by local temperature regimes, and are therefore constant, irrespective of site quality (nutrient status), for any particular climatic zone. Culm-duration (life-time) is not significantly influenced by climate and the ratio culms/rhizomes provides a useful indicator of comparative productivity. Unlike community biomass, productivity can be limited by site quality.     

Temperature response of the cell cycle of Haplopappus gracilis in suspension culture and its significance to the G1 transition probability model

The effects of temperature on the cell cycle of Haplopappus gracilis suspension cultures were analysed by the fraction of labelled mitoses method. Sphase in these cultures shows a different temperature optimum as compared to optima derived for G₂ and mitosis. G₁ phase has a much lower Q₁₀ than the other cell cycle phases and shows no temperature optimum between 22 and 34° C. These results are discussed in relation to a transition probability model of the cell cycle proposed by Smith and Martin (Proc. Natl. Acad. Sci. USA 70, 1263–1267, 1973), in which each cell has a time independent probability of initiating the transition into another round of DNA replication and division. The implications of such a model for cell cycle analysis are discussed and a tentative model for a probabilistic transition trigger is advanced.Abbreviations FLM Fraction of labelled mitoses - T_B Total B-phase     

High-density photoautotrophic algal cultures: design, construction, and operation of a novel photobioreactor system

A photobioreactor system has been designed, constructed and implemented to achieve high photosynthetic rates in high-density photoautotrophic algal cell suspensions. This unit is designed for efficient oxygen and biomass production rates, and it also can be used for the production of secreted products. A fiber-optic based optical transmission system that is coupled to an internal light distribution system illuminates the culture volume uniformly, at light intensities of 1.7 mW/cm(2) over a specific surface area of 3.2 cm(2)/cm(3). Uniform light distribution is achieved throughout the reactor without interfering with the flow pattern required to keep the cells in suspension. An on-line ultrafiltration unit exchanges spent with fresh medium, and its use results in very high cell densities, up to 10(9) cells/mL [3% (w/v)] for eukaryotic green alga chlorella vulgaris. DNA histograms obtained form flow cytometric analysis reveal that on-line ultrafiltration influences the growth pattern. Prior to ultrafiltration the cells seem to have at a particular point in the cell cycle where they contain multiple chromosomal equivalents. Following ultrafiltration, these cells divide, and the new cells are committed to division so that cell growth resumes. The Prototype photobioreactor system was operated both in batch and in continuous mode for over 2 months. The measured oxygen production rate of 4-6 mmol/L culture h under continuous operation is consistent with the predicted performance of the unit for the provided light intensity.     

Chloroplast phosphoribulokinase associates with yeast phosphoriboisomerase in the presence of substrate

Pea chloroplastic phosphoribulokinase and yeast phosphoriboisomerase partition independently of one another in a two-phase polyethyleneglycol, dextran system, but apparent interaction is seen when ribose-5-phosphate is added to the two-phase system. It appears that the pea leaf of kinase recognizes yeast isomerase when it is carrying metabolite.     

Cell Cycling of Astrocytes and Their Precursors in Primary Cultures: A Mevalonate Requirement Identified in Late G1, but Before the G1/S Transition, Involves Polypeptides

The relationship between mevalonate and cell cycling was investigated in developing glial cells. Primary cultures of newborn rat brains were serum-depleted (0.1%, vol/vol) for 48 h on days 4-6 in vitro, then returned to 10% calf serum (time 0). After 48 h, 70-80% of the cells were glial fibrillary acidic protein (GFAP)-negative by indirect immunofluorescence; 79 +/- 7% were GFAP-positive after an additional 3 days. Serum shift-up resulted in 12 h of quiescence, and then by 20 h (S phase) in increased proportions of cells synthesizing DNA (from 15 +/- 6% to 75 +/- 4% by bromodeoxyuridine immunofluorescence at 12 h and 20 h, respectively) and rates of DNA synthesis (42 +/- 6 versus 380 +/- 32 cpm/micrograms of protein/h of [3H]thymidine uptake). Additional mevalonate (25 mM) for 30 min at 10 h reversed the inhibition of DNA synthesis apparent with mevinolin (150 microM), an inhibitor of mevalonate synthesis, present from time 0. Cycloheximide added simultaneously with mevalonate prevented this reversal of inhibition. To cause arrest at G1/S, cultures were exposed to hydroxyurea between 10 and 22 h. By 3 h after hydroxyurea removal, bromodeoxyuridine-labeled nuclei increased from 0% to 75 +/- 9%, and DNA synthesis increased 10-fold. Mevinolin failed to inhibit these increases. Thus, primary astroglial precursors stimulated to progress through the cell cycle express a mevalonate requirement in late G1, but before the G1/S transition. The effect of mevalonate was characterized further as being brief (30 min) and as requiring polypeptides.     

Alternation between attachment mechanisms by limpets in the field

The attachment mechanism used by limpets in the rocky, wave-swept intertidal zone of California was determined during high tide and low tide. The two mechanisms that limpets are known to use, suction and glue-like adhesion, were distinguished by measuring the limpets' attachment forces in shear and by staining for glue-like residues where the limpets had been attached. The results show that ≈ 73% of limpets at high tide use suction, while the rest use glue-like adhesion. Conversely, ≈ 75% of limpets at low tide use glue-like adhesion, while the rest use suction. The normal tenacity of limpets was also measured at high and low tide. The mean tenacity at high tide was significantly less than that at low tide. From these data it was estimated that the mean tenacity of glue-like adhesion is ≈ 0.23 MN·m⁻² and the mean tenacity of suction adhesion is ≈ 0.09 MN·m⁻². It is hypothesized that the cycle of alternating attachment mechanisms is linked to the limpets foraging cycles.     

Analysis of cellular interactions in density-dependent inhibition of 3T3 Cell proliferation

Subpopulations of different proliferative status are determined during cell-density dependent proliferation of 3T3 cells. From these data the probability of conversion of proliferative to quiescent cells is derived and found to correlate well with published data on binding of growth-inhibiting factors secreted from growth-inhibited cells.Based on material presented at the Symposium Intercellular Communication Stuttgart, September 16–17, 1982     

A regression analysis of the summer population dynamics of Polyarthra vulgaris in a northern Michigan bog lake

The population dynamics of a planktonic rotifer (Polyarthra vulgaris) were examined in a brown water, acid lake in northern Michigan, U.S.A. Predation by Chaoborus punctipennis and low food (Navicula spp. and Cyclotella spp.) concentrations were the main factors limiting P. vulgaris populations of all factors examined. The data presented here support a hypothesis for zooplankton limitation by an invertebrate predator.     

Correlation of rotifer associations in a chain of lakes fed by reclaimed sewage

The quantitative composition of the rotifer fauna of seven interconnected lakes located in southern California was studied in a preliminary investigation. The lakes are fed by reclaimed sewage, and the water quality increases rapidly from the first lake, barely able to support life, to a series of lovely lakes stocked with fish and utilized for recreation. The Shannon index of rotifer population diversity showed an appropriate increase. Up to 24 – 28 different rotifer taxa in each lake provided a rich community dominated by Brachionids, Keratella and Trichocerca. The diversity and quantity of the rotifer association then declined again in the last lake. Comparison of the lakes using the Bray and Curtis index of similarity and cluster analysis supported the picture of a lake-chain evolution, a biotic development showing a maximum curve.