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51.
In this paper we present a conceptual model of integrated plant-soil interactions which illustrates the importance of identifying the primary belowground feedbacks, both positive and negative, which can simultaneously affect plant growth responses to elevated CO2. The primary negative feedbacks share the common feature of reducing the amount of nutrients available to plants. These negative feedbacks include increased litter C/N ratios, and therefore reduced mineralization rates, increased immobilization of available nutrients by a larger soil microbial pool, and increased storage of nutrients in plant biomass and detritus due to increases in net primary productivity (NPP). Most of the primary positive feedbacks share the common feature of being plant mediated feedbacks, the only exception being Zak et al.'s hypothesis that increased microbial biomass will be accompanied by increased mineralization rates. Plant nutrient uptake may be increased through alterations in root architecture, physiology, or mycorrhizal symbioses. Further, the increased C/N ratios of plant tissue mean that a given level of NPP can be achieved with a smaller supply of nitrogen.Identification of the net plant-soil feedbacks to enhanced productivity with elevated CO2 are a critical first step for any ecosystem. It is necessary, however, that we first identify how universally applicable the results are from one study of one ecosystem before ecosystem models incorporate this information. The effect of elevated CO2 on plant growth (including NPP, tissue quality, root architecture, mycorrhizal symbioses) can vary greatly for different species and environmental conditions. Therefore it is reasonable to expect that different ecosystems will show different patterns of interacting positive and negative feedbacks within the plant-soil system. This inter-ecosystem variability in the potential for long-term growth responses to rising CO2 levels implies that we need to parameterize mechanistic models of the impact of elevated CO2 on ecosystem productivity using a detailed understanding of each ecosystem of interest. 相似文献
52.
We conducted a set of in situ incubations to evaluate patterns of N availability among dominant land uses in the shortgrass
steppe region of Colorado, USA, and to assess recovery of soil fertility in abandoned fields. Replicated 30 d incubations
were performed in 3 sets of native (never cultivated), abandoned (cultivated until 1937), and currently cultivated, fallow
fields. Net N mineralization and the percentage of total N that was mineralized increased in the order: native, abandoned,
cultivated. Higher soil water content in fallow fields is the most likely reason for greater mineralization in cultivated
fields, while higher total organic C and C/N ratios in native and abandoned fields may explain differences in mineralization
between these land uses. Recovery of soil organic matter in abandoned fields appears to involve accumulation of soil C and
N under perennial plants, but probable methodological artifacts complicate evaluation of the role of individual plants in
recovery of N availability. Higher N mineralization and turnover in cultivated fields may make them more susceptible to N
losses; recovery of N cycling in abandoned fields appears to involve a return to slower N turnover and tighter N cycling similar
to native shortgrass steppe. 相似文献
53.
J. Hassink 《Plant and Soil》1995,176(1):71-79
Different methods for estimating the non-fertilizer N supply (NFNS) of mineral grassland soils were compared. NFNS was defined as the N uptake on unfertilized plots. The potential mineralization rate (0–12 weeks), macroorganic matter and active microbial biomass (determined by the substrate-induced respiration method; SIR) were correlated positively with NFNS. The difference between the actual soil organic N or microbial N content (determined by the fumigation incubation method) and their contents under equilibrium conditions ( org. N and MB-N), however, gave the best estimations of NFNS. For field conditions the best estimation for NFNS was: NFNS (kg N ha–1 yr–1)=132.3+42.1× org. N (g kg–1 soil; r=0.80). This method is based on the observation that, under old grassland swards, close relationships exist between soil texture and the amounts of soil organic N and microbial N. These relationships are assumed to represent equilibrium conditions as under old swards under constant management, the gain in soil organic N and microbial N equals the losses. Soils under young grassland and recently reclaimed soils contained less soil organic N and microbial N. In such soils the amounts of organic N and microbial N increase with time, which is reflected in a lower NFNS. The annual accumulation of organic and microbial N gradually becomes smaller until organic N, microbial N and NFNS reach equilibrium. The main advantage of the difference method in comparison with the other methods is its speed and simplicity.FAX no: +31 50337291 相似文献
54.
We examined the effects of the conversion of tropical forest to pasture on soil organic matter (SOM) origin and quality along
a chronosequence of sites, including a primary forest and six pastures. Bulk soil samples received a physical size-fractionation
treatment to assess the contribution of each compartment to total SOM pool. Besides a general increase in total C and N stocks
along the chronosequence, we observed a reduction of the relative contribution of the coarser fractions to total soil C content,
and an increased concentration in the finer fractions. The origin of the C in each size fraction was established from measurements
of13C abundance. After 80 years about 93% of the C in the least humified fraction of the top 10 cm of soil was of pasture origin,
while in the most humified it was 82%. Chemical analyses indicated that the fine silt and coarse clay fractions contained
the most refractory carbon. 相似文献
55.
Summary This note surveys the current knowledge of the existence of acute zones in insects' eyes in relation to beeflower interactions.We suggest some broad generalizations correlating the size, use, structure and presentation of flowers with the foraging behaviour, body size and eye design of bees.Special emphasis is given to the differences between small and large bees and the architecture of flowers that they use. 相似文献
56.
Effects of manipulation of food supply on estuarine meiobenthos 总被引:1,自引:0,他引:1
A comparative mesocosm experiment was carried out to determine the effects of natural foods of different quality and quantity on the structure of natural meiobenthic communities collected in undisturbed sediment from the polluted Westerschelde and the comparatively undisturbed Gironde estuaries. Nematode communities are more diverse and species rich in the latter estuary. The organic matter or foods used were phytoplankton, green alga, salt marsh plant detritus and leaf litter detritus which were added at three dose rates including a high dose. There was no change in community structure in response to the treatments in either of the estuarine meiobenthic communities. Analysis of all the results from this experiment indicate that the food quantity manipulations had almost no effect on the deposit feeding meiofauna. It may be that the reserves of organic matter within the sediment were sufficient to satisfy their dietary requirements for the duration of the experiment. The abundance of diatom/epigrowth feeding nematodes which were initially dominant in the Gironde, declined substantially suggesting that they may have been food limited since diatoms were not among the sources of organic matter added to the mesocosm. There was no specific response to the five different types of organic matter added to the mesocosm 相似文献
57.
It is widely held that there was a phosphate compound in prebiotic chemistry that played the role of adenosine triphosphate and that the first living organisms had ribose-phosphate in the backbone of their genetic material. However, there are no known efficient prebiotic synthesis of high-energy phosphates or phosphate esters. We review the occurrence of phosphates in Nature, the efficiency of the volcanic synthesis of P4O10, the efficiency of polyphosphate synthesis by heating phosphate minerals under geological conditions, and the use of high-energy organic compounds such as cyanamide or hydrogen cyanide. These are shown to be inefficient processes especially when the hydrolysis of the polyphosphates is taken into account. For example, if a whole atmosphere of methane or carbon monoxide were converted to cyanide which somehow synthesized polyphosphates quantitatively, the polyphosphate concentration in the ocean would still have been insignificant. We also attempted to find more efficient high-energy polymerizing agents by spark discharge syntheses, but without success. There may still be undiscovered robust prebiotic syntheses of polyphosphates, or mechanisms for concentrating them, but we conclude that phosphate esters may not have been constituents of the first genetic material. Phosphoanhydrides are also unlikely as prebiotic energy sources.
Correspondence to: S.L. Miller 相似文献
58.
The catalytic properties of bovine liver catalase have been investigated in organic solvents. In tetrahydrofuran, dioxane, and acetone (all containing 1% to 3% of water), the enzyme breaks down tert-butyl hydroperoxide several fold faster than in pure water. Furthermore, the rate of catalase-catalyzed production of tert-butanol from tert-butyl hydroperoxide increases more than 400-fold upon transition from aqueous buffer to ethanol as the reaction medium. The mechanistic rationale for this striking effect is that in aqueous buffer the rate-limiting step of the enzymatic process involves the reduction of catalase's compound I by tert-butyl hydroperoxide. In ethanol, and additional step in the reaction scheme becomes available in which ethanol, greatly outcompeting the hydroperoxide, is oxidized by compound I regenerating the free enzyme. In solvents, such as acetonitrile or tetrahydrofuran, which themselves are not oxidizable by compound I, catalase catalyzes the oxidation of numerous primary and secondary alcohols with tert-butyl hydroperoxide to the corresponding aldehydes or ketones. The enzymatic oxidation of some chiral alcohols (2,3-butanediol, citronellol, and menthol) under these conditions occurs enantioselectively. Examination of the enantioselectivity for the oxidation of 2,3-butanediol in a series of organic solvents reveals a considerable solvent dependence. (c) 1995 John Wiley & Sons, Inc. 相似文献
59.
The effective diffusion coefficient and the distribution constant for small molecules in calcium-alginate gel beads 总被引:2,自引:0,他引:2
The effective diffusion coefficient, D(e), and the distribution constant, K(i), for selected mono- and disaccharides and organic acids were determined in homogeneous calcium-alginate gel with and without entrapped bacteria. Results were obtained from transient concentration changes in well-stirred solutions of limited volume, in which the gel beads were suspended. The effective diffusioncoefficients and the distribution constants were estimated by fitting mathematical model predictions to the experimental data using a nonlinear model fitting program (MODFIT). Both single solute diffusion and multiple solute diffusion were performed. A small positive effect was obtained onthe values of D(e) for the system of multiple solute diffusion; however, the values of K(i) were not significantly influenced. For the nine solutes tested, D(e) for 2% Ca-alginate gel beads was found to be approximately 85% of the diffusivity measured in water. The effects on D(e) and K(i), for lactose and lactic acid were determined for variations of alginate concentration, pH, temperature, and biomass content in the beads. D(e) decreased linearly for both lactose and lactic acid with increasing cell concentration in the Ca-alginate gel. K(i), was constant for both lactose and lactic acid with increasing cell concentration. D(e) was significantly lower at pH 4.5 than at pH 5.5 and 6.5 for both lactose and lactic acid. Furthermore, D(e) seemed to decrease with increased alginate concentration in the range of 1% to 4%. The diffusion rate increased with increasing temperature, and the activation energy for the diffusion process for both lactose and lactic acid was constant in the temperature range tested. (c) 1995 John Wiley & Sons Inc. 相似文献
60.
Polyphenoloxidases immobilized in organic gels: Properties and applications in the detoxification of aromatic compounds 总被引:5,自引:0,他引:5
Gelatine gels originate from water in oil microemulsions in which the ternary system consists of isooctane/ sulfosuccinic acid bis [2-ethyl hexyl] ester/water; the solubilization of gelatin in the water pool of these microemulsions transforms them into viscous gels in which it is possible to cosolubilize various reactive molecules. These gels were used to immobilize two phenoloxidases, a laccase from Trametes versicolor and a tyrosinase from mushroom. The best balance between gel retention and catalytic activity was reached at a gelatine concentration of 2.5% (w/v) in the case of tyrosinase, while laccase immobilization was independent of gelatine concentration. Both enzymes kept the same optimum pH as the corresponding soluble controls, while a partial loss of activity was observed when they were immobilized. Immobilized enzymes showed an increased stability when incubated for several days at 4 degrees C with a very low release from the gels in the incubation solutions. The immobilization of tyrosinase and of laccase enhanced stability to thermal inactivation. Furthermore, gel-entrapped tyrosinase was almost completely preserved from proteolysis: more than 80% of the activity was maintained, while only 25% of the soluble control activity was detected after the same proteolytic treatments. A column packed with gel-immobilized tyrosinase was used to demonstrate that enzymes immobilized with this technique may be reused several times in the same reaction without loosing their efficiency. Finally, gel-entrapped tyrosinase and laccase were capable of removing naturally occurring and xeno-biotic aromatic compounds from aqueous suspensions with different degrees of efficiency. (c) 1995 John Wiley & Sons, Inc. 相似文献