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201.
Luisa Zupin Rossella Gratton Francesco Fontana Libera Clemente Lorella Pascolo Maurizio Ruscio Sergio Crovella 《Journal of biophotonics》2021,14(4):e202000496
The study of any intervention able to counteract SARS-CoV-2 pandemic is considerably envisaged. It was previously shown, in in vitro models of infections, that the LED blue light is able to decrease the viral load of HSV-1 and ZIKV. In our study, LED photobiomodulation therapy (PBMT) at blue wavelengths (450, 454 and 470 nm) was tested in an in vitro model of SARS-CoV-2 infection, employing three experimental settings: SARS-CoV-2 was irradiated and then transferred to cells; already infected cells were irradiated; cells were irradiated prior to infection. A decrement of the viral load was observed when previously infected cells were irradiated with all three tested wavelengths and relevant effects were registered especially at 48 hours post-infection, possibly suggesting that the blue light could interfere with the intracellular viral replication machinery. Our in vitro findings could represent the starting point for translational applications of PBMT as a supportive approach to fight SARS-CoV-2. 相似文献
202.
203.
Seventy-eight heterocyclic compounds, which included derivatives of furan, isoxazole, pyridine, pyrrole, and thiophene, were tested in an in vitro system for their antimicrobial activity and their strain differentiating properties against three strains of Trypanosoma cruzi (Brazil, Corpus Christi, Tulahuen) and for their effect, in tissue culture, on African Green Monkey Kidney Cells—strain Vero. Forty-eight compounds were 75–100% effective in immobilizing trypomastigote forms of the three strains tested, and 29 of these were rated nondestructive to African Green Monkey Kidney (AGMK) cells. Of these 29 compounds, 12 gave variable results ranging from complete immobilization of one strain to no apparent effect on the motility of one or two of the other strains. Nine of the compounds rated harmless to AGMK fibroblasts are proposed as a basis for constructing molecules which might be active chemotherapeutic agents against various strains of T. cruzi. 相似文献
204.
Cell-free extracts of Trypanosoma cruzi, Leishmania donovani, and L. mexicana, cultivated in a medium supplemented with 5% fetal calf serum, contain a factor that induces lysis of mammalian red blood cells and Vero cells. All the lytic activity was found in the insoluble fraction of parasite extracts obtained after centrifugation at 100,000g for 2 hr. The lytic agent is pronase, trypsin, and temperature resistant. The optimum pH of the lytic effect is pH 6.5. Normal red blood cells of several mammalian species had different sensitivities to the lytic agent. The lipid phase of T. cruzi extract contains the total lytic activity. Albumins of different animal species at 1 mg/ml, completely inhibit the lytic activity of parasite extracts. 相似文献
205.
SYNOPSIS. A video technic is described that permits a quantification of the degree of attraction of Trypanosoma cruzi trypomastigotes to vertebrate cells in vitro. Bovine embryo skeletal muscle cells (BESM), HeLa cells and Vero cells all attract a myotropic strain of T. cruzi trypomastigotes. BESM cells, however, are 2-fold more attractive to trypomastigotes than HeLa cells and 10-fold more attractive than Vero cells. Heat-inactivation of BESM cells abolishes their ability to respire and also to attract T. cruzi trypomastigotes. As there is no difference in the endogenous oxygen consumption between BESM, HeLa, and Vero cells, it is unlikely that differences in the attraction of trypomastigotes to the 3 cell types are due to variations in the magnitude of pO2 or pCO2 gradients in the milieu around the cells. 相似文献
206.
W. S. Ramsey W. Hertl E. D. Nowlan N. J. Binkowski 《In vitro cellular & developmental biology. Plant》1984,20(10):802-808
Summary Photoelectron spectroscopic examination of treated plastic surfaces showed that surface oxidation, primarily as carboxyl groups,
was responsible for formation of good growth surfaces. Gas-plasma studies indicated that only very short exposures were required
and that the effect was confined to a thin surface layer that produced adhesive surfaces. Highly adhesive surfaces were produced
using oxidizing chemicals. Studies with a polymeric ester demonstrated the importance of unesterified carboxyl groups for
high adhesiveness. 相似文献
207.
Toru Nishikawa Jun Fujii Shin-ichi Yoshida Takashi Yutsudo 《FEMS microbiology letters》1999,178(1):13-18
Escherichia coli clones expressing recombinant Shiga toxin (Stx)1-A and recombinant Stx1-B subunits, were established. Culture supernatants of these clones were examined for inhibitory activity on in vitro protein synthesis using luciferase as a reporter enzyme. Culture supernatant of the clone expressing Stx1-A, but not Stx1-B, showed the inhibitory activity. Neither recombinant Stx1-A nor Stx1-B showed Vero cell cytotoxicity. For reconstitution of biologically active toxin, the culture supernatants of the Stx1-A clone and the Stx1-B clone were mixed. The reconstituted recombinant Stx1 showed both Vero cell cytotoxicity and inhibition of in vitro protein synthesis. 相似文献
208.
Summary A mixture containing an ultrafiltrate fraction (UF) of bovine colostrum (6.7%), adult bovine serum (BS) (1%), and human holo-transferrin
(hTF) (5 mg/liter) was developed for cultivation of Chinese hamster ovary cells (CHO-K1) and African green monkey kidney cells
(Vero). The growth-supporting activity of the mixture (UF/BS/hTF) was comparable to that of 1 to 10% fetal bovine serum (FBS)
and considerably better than 1 to 2% BS. Cells could be directly seeded from FBS-supplemented medium to UF/BS/hTF-supplemented
medium without any weaning period, even at initial plating density of 1700 cells/ml. Vero and CHO-K1 cells were cultivated
in UF/BS/hTF-supplemented media for up to 43 days without any apparent reduction in growth. The UF/BS/hTF mixture could also
be used as a freezing medium. Cells were passaged twice in the mixture, frozen, and stored at liquid N2 for 11 wk. After thawing, the viability of Vero and CHO-K1 cells was reduced 13 and 7%, respectively, and both cell lines
started to grow well. Additional hTF could be replaced with bovine holo-transferrin, although a high concentration (150 mg/liter)
should be used for CHO-K1 cells. The results suggest that the UF/BS/hTF mixture provides a new economical alternative to FBS
in cultivation of Vero and CHO-K1 cells in the presence of reduced protein amounts. 相似文献