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排序方式: 共有59条查询结果,搜索用时 15 毫秒
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Cockram J Chiapparino E Taylor SA Stamati K Donini P Laurie DA O'sullivan DM 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(7):993-1001
In barley, variation in the requirement for vernalization (an extended period of low temperature before flowering can occur) is determined by the VRN-H1, -H2 and -H3 loci. In European cultivated germplasm, most variation in vernalization requirement is accounted for by alleles at VRN-H1 and VRN-H2 only, but the range of allelic variation is largely unexplored. Here we characterise VRN-H1 and VRN-H2 haplotypes in 429 varieties representing a large portion of the acreage sown to barley in Western Europe over the last 60 years. Analysis of genotype, intron I sequencing data and growth habit tests identified three novel VRN-H1 alleles and determined the most frequent VRN-H1 intron I rearrangements. Combined analysis of VRN-H1 and VRN-H2 alleles resulted in the classification of seventeen VRN-H1/VRN-H2 multi-locus haplotypes, three of which account for 79% of varieties. The molecular markers employed here represent powerful diagnostic tools for prediction of growth habit and assessment of varietal purity. These markers will also allow development of germplasm to test the behaviour of individual alleles with the aim of understanding the relationship between allelic variation and adaptation to specific agri-environments. 相似文献
34.
Identification of quantitative trait loci and associated candidate genes for low-temperature tolerance in cold-hardy winter wheat 总被引:4,自引:0,他引:4
Båga M Chodaparambil SV Limin AE Pecar M Fowler DB Chibbar RN 《Functional & integrative genomics》2007,7(1):53-68
Low-temperature (LT) tolerance is an important economic trait in winter wheat (Triticum aestivum L.) that determines the plants’ ability to cope with below freezing temperatures. Essential elements of the LT tolerance mechanism are associated with the winter growth habit controlled by the vernalization loci (Vrn-1) on the group 5 chromosomes. To identify genomic regions, which in addition to vrn-1 determine the level of LT tolerance in hexaploid wheat, two doubled haploid (DH) mapping populations were produced using parents with winter growth habit (vrn-A1, vrn-B1, and vrn-D1) but showing different LT tolerance levels. A total of 107 DH lines were analyzed by genetic mapping to produce a consensus map of 2,873 cM. The LT tolerance levels for the Norstar (LT50=−20.7°C) × Winter Manitou (LT50=−14.3°C) mapping population ranged from −12.0 to −22.0°C. Single marker analysis and interval mapping of phenotyped lines revealed a major quantitative trait locus (QTL) on chromosome 5A and a weaker QTL on chromosome 1D. The 5A QTL located 46 cM proximal to the vrn-A1 locus explained 40% of the LT tolerance variance. Two C-repeat Binding Factor (CBF) genes expressed during cold acclimation in Norstar were located at the peak of the 5A QTL. 相似文献
35.
36.
Physical mapping of the Vrn-A1 and Fr1 genes on chromosome 5A of wheat using deletion lines 总被引:1,自引:0,他引:1
J. Sutka G. Galiba A. Vagujfalvi B. S. Gill J. W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):199-202
Homozygous deletion lines of wheat for 5AL, generated in the variety ‘Chinese Spring’, were tested for flowering time without
vernalization and for frost resistance after cold hardening. It was found that the Vrn-A1 gene for vernalization requirement mapped between breakpoints 0.68 and 0.78, whilst the frost resistance gene Fr1 was flanked by deletion breakpoints 0.67 and 0.68. This confirms previous evidence that these genes are linked but are not
the pleiotropic effect of a single gene. A comparison between the physical and genetic maps for Vrn-A1 and Fr1 shows that the linear order is identical. These results indicate that cytogenetically based physical maps of Vrn-A1 and Fr1 loci, together with genetic maps, could be useful in the further study of genome synteny and in elaborating a gene cloning
strategy.
Received: 16 November 1998 / Accepted: 28 November 1998 相似文献
37.
In vivo and in vitro flowering response of chicory (Cichorium intybus L.): influence of plant age and vernalization 总被引:3,自引:0,他引:3
Chicory plants (Cichorium intybus L. var foliosum cv Flash) were tested with and without a 4-week-long cold treatment for in vivo and in vitro flowering potential every 2 weeks during the growing season. One hundred percent of the plants harvested 112 days or later
after sowing and then vernalized flowered in vivo. In vitro, no vernalization was needed to initiate flowering-stems on chicory explants taken from roots of 100 days old and older.
5-Azacytidine, a DNA demethylation agent, increased the flowering percentage on explants from young, vernalized roots but
could not induce more than 15% flowering on young, nonvernalized roots. The greater flowering potential of chicory root explants
in vitro when compared to plants of the same age tested in vivo was clearly established. This result suggests that some negative control on flowering was removed when root explants were
excised and the main plant body discarded.
Received: 31 August 1998 / Revision received: 27 October 1998 / Accepted: 10 November 1998 相似文献
38.
Epigenetic regulation is important for maintaining gene expression patterns in multicellular organisms. The Polycomb Group (PcG) proteins form several complexes with important and deeply conserved epigenetic functions in both the plant and animal kingdoms. The plant Polycomb Repressive Complex 2 (PRC2) contains four core proteins, Enhancer of Zeste (E(z)), Suppressor of Zeste 12 (Su(z)12), Extra Sex Combs (ESC), and Multicopy Suppressor of IRA 1 (MSI1), and functions in many developmental transitions. In some plant species, including rice and Arabidopsis, duplications in the core PRC2 proteins allow the formation of PRC2s with distinct developmental functions. In addition, members of the plant specific VEL PHD family have been shown to associate with the PRC2 complex in Arabidopsis and may play a role in targeting the PRC2 to specific loci. Here we examine the evolution and expression of the PRC2 and VEL PHD families in Aquilegia, a member of the lower eudicot order Ranunculales and an emerging model for the investigation of plant ecology, evolution and developmental genetics. We find that Aquilegia has a relatively simple PRC2 with only one homolog of Su(z)12, ESC and MSI1 and two ancient copies of E(z), AqSWN and AqCLF. Aquilegia has four members of the VEL PHD family, three of which appear to be closely related to Arabidopsis proteins known to associate with the PRC2. The PRC2 and VEL PHD family proteins are expressed at a relatively constant level throughout Aquilegia vulgaris development, with the VEL PHD family and MSI1 expressed at higher levels during and after vernalization and in the inflorescence. Both AqSWN and AqCLF are expressed in Aquilegia endosperm but neither copy is imprinted. 相似文献
39.
The transition from the vegetative to reproductive stage followed by inflorescence is a critical step in plant life; therefore, studies of the genes that influence flowering time have always been of great interest to scientists. Flowering is a process controlled by many genes interacting mutually in a genetic network, and several hypothesis and models of flowering have been suggested so far. Plants in temperate climatic conditions must respond mainly to changes in the day length (photoperiod) and unfavourable winter temperatures. To avoid flowering before winter, some plants exploit a specific mechanism called vernalization. This review summarises current achievements in the study of genes controlling flowering in the dicot model species thale cress (Arabidopsis thaliana), as well as in monocot model species rice (Oryza sativa) and temperate cereals such as barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.). The control of flowering in crops is an attractive target for modern plant breeding efforts aiming to prepare locally well-adapted cultivars. The recent progress in genomics revealed the importance of minor-effect genes (QTLs) and natural allelic variation of genes for fine-tuning flowering and better cultivar adaptation. We briefly describe the up-to-date technologies and approaches that scientists may employ and we also indicate how these modern biotechnological tools and “-omics” can expand our knowledge of flowering in agronomically important crops. 相似文献
40.
A. E. Limin D.B. Fowler 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):277-281
Reduced cell size is an important adaptive feature in plant response to environmental stresses. The objectives of the present
study were to determine the inheritance and location of genes controlling cell size and to establish the relationship between
cell size, low-temperature (LT) tolerance, and growth habit as determined by the Vrn loci in wheat. Guard cell length was measured in F1, F2, andF2-derived F3 populations from parents ranging widely in cell size and in the Chinese Spring/ Cheyenne (CS/CNN) chromosome substitution
series. The cell size of F1 hybrids was similar to the parental midpoint and the F2 frequency distribution was symmetrical about the mean indicating that cell size was determined by additive gene action with
little or no dominance. It appears that there are several genes involved since none of the F2 progeny had a cell size as large or as small as the parental mean range. The cell size of the homozygous spring and winter
lines from F2-derived F3 populations fell into two distinct groups that were related to plant growth habit. Large cell size was associated with the
spring-habit alleles (Vrn-A1) and small cell size was associated with the winter-habit alleles (vrn-A1) on chromosome 5A. Analyses of the CS/CNN chromosome substitution series showed that CNN chromosomes 5A and 5B both reduced
cell size without changing the growth habit, indicating that growth habit per se does not determine cell size. The group-5
chromosomes therefore appear to carry homoeologous alleles with major effects on cell size in wheat. This places cell-size
control and many other low-temperature (LT) tolerance associated characters in close proximity to the vrn region of the group-5 chromosomes.
Received: 17 August 2000 / Accepted: 20 November 2000 相似文献