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971.
Synopsis Changes in the daily appetite and weekly growth rates of individual adult minnows,Phoxinus phoxinus, on ad libitum rations were recorded before and after they had experienced 4 or 16 days of food restriction. Feeding levels during the restriction periods were either starvation or a maintenance ration. The latter was estimated from a previously determined regression model. Water temperature was 15°C and the photoperiod 9L15D in all experiments. The mean weight of fish used ranged from 1.06 to 2.15 g. The 4 day restriction had no detectable effects on appetite or growth. After the 16 day restriction, the minnows showed hyperphagia and had increased specific growth rates and growth efficiencies compared with control fish. The compensatory increases in appetite and growth were not sustained and within three weeks had declined to levels not significantly different from those of the control fish. At the end of the experiments, there were no significant differences between the mean weights or cumulative food consumption of the restricted and control groups. The results suggest that adult minnows regulate their appetite and growth rate in relation to their previous nutritional history. 相似文献
972.
Habitat associations with topography and canopy structure of tree species in a tropical montane forest on Mount Kinabalu,Borneo 总被引:1,自引:0,他引:1
Habitat associations with topography and canopy structure of 42 abundant tree species were studied in a 2.74-ha plot of tropical
montane forest on Mount Kinabalu, Borneo. Many of these species belong to the same higher taxa including eight families and
four genera. Analysis of intraspecific spatial distributions for stems ≥ 10 cm diameter revealed that 28 species (including
all six species of Fagaceae) showed aggregated distributions at the 100-m2 and/or 400-m2 scales, and that 20 species showed habitat associations with topography by torus-translation tests; 17 species showed both
characteristics. Species' associations with the local canopy structure were characterized by crown position index (CPI), which
was defined relative to neighbour trees. The CPI differed greatly among individual stems at 10–40 cm diameter, and 19 species
showed significantly different frequencies of crowns exposed vertically versus those shaded beneath the canopy. Mean growth
rates at 10–40 cm diameter and size distributions of species were not related to topographic associations, but were explained
by the associations with canopy structure; species with more exposed crowns grew faster and had less positively skewed distributions.
Diversity in habitat associations was manifest between two genera (Syzygium and Tristaniopsis) in the family Myrtaceae and among species in these genera, but was less evident in other families and two genera (Garcinia and Lithocarpus).
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
973.
974.
从人良性增生前列腺组织中经硫酸铵沉淀和肝素-琼脂糖凝胶层析纯化出人前列腺生长因子(hPGF),纯化倍数约1000倍,SDS-PAGE和等电聚焦电泳示分子量约为17kD、等电点同标准bFGF,利用分离培养的人前列腺间质成纤维细胞进行活性鉴定,发现以1.3~1.7mol/LNaCl洗脱部分为hPGF,活性最高,对间质成纤维细胞有显著刺激增殖作用。 相似文献
975.
Experiments were conducted in greenhouse, growth chamber, and laboratory conditions to determine the effect of ammonium sulfate [(NH4)2SO4] on the phytotoxicity, foliar uptake, and translocation of imazamethabenz on wild oat. Rates of (NH4)2SO4 up to 5% (w/v) applied with a greenhouse sprayer did not affect the phytotoxicity of the herbicide when the mix was applied at the one- to two-leaf stage. However, inclusion of 1 and 2% (NH4)2SO4 increased the phytotoxicity of the herbicide when the mix was sprayed at the two- to three-leaf, or the three- to four-leaf stage. At 10%, (NH4)2SO4 decreased the phytotoxicity of the sublethal dosage of the herbicide. When the herbicide was applied as individual drops to the growth chamber-grown plants, inclusion of (NH4)2SO4 at 1% did not affect phytotoxicity as measured by shoot growth. The presence of (NH4)2SO4 did not affect the amount of imazamethabenz retained by wild oat foliage, but it decreased [14C]imazamethabenz absorption, slightly antagonized acropetal translocation, and increased the basipetal translocation of [14C]imazamethabenz. It was concluded that application methods greatly modify the effect of (NH4)2SO4 on imazamethabenz phytotoxicity. Herbicide absorption and translocation as determined by one method do not necessarily represent the absorption and translocation patterns when different application methods are used. Absorption and translocation were not the factors that were responsible for the observed effect of (NH4)2SO4 on the herbicide phytotoxicity.Abbreviations SC
suspension concentrate 相似文献
976.
Chang WC Shyu WJ Shi GY Lin MT Jen CJ Wing LY Tang MJ Wu HL 《Journal of biomedical science》1996,3(1):59-66
Treatment of cultured bovine carotid artery endothelial cells with 0.1 µM human plasmin has been reported to induce a receptor-mediated short burst of arachidonate release, which is a pertussis toxin-sensitive and extracellular calcium-dependent reaction. Plasmin-induced calcium influx in cells was significantly inhibited by pretreatment with pertussis toxin, indicating that the former was coupled with a pertussis toxin-sensitive guanosine 5-triphosphate (GTP)-binding protein. Plasmin significantly induced the formation of lysophosphatidylcholine but not lysophosphatidylethanolamine. A cellular phospholipase A2 with an arachidonyl specificity at the sn-2 position of phosphatidylcholine, which required submicromolar calcium, was identified as a cytosolic phospholipase A2 by immunoblot analysis. By a cell-free enzyme activity assay and immunoblot analysis, plasmin was found to induce a translocation of the cytosolic phospholipase A2 from the cytosol to the membrane. Taken together, the results suggest that plasmin bound to its putative receptor and activated a GTP-binding protein coupled to calcium influx channel, followed by translocation and activation of cytosolic phospholipase A2 in endothelial cells. 相似文献
977.
本文利用PCR技术,对人肿瘤坏死因子α(hTNFα)基因进行了改造,并将其与人白细胞介素-6(hIL-6)成熟肽编码区cDNA进行融合,构建了5′IL-6-TNF△融合蛋白的表达质粒pBVIL6-TNFA△。DNA序列分析证明,PCR扩增片段核苷酸序列与引物设计序列及相应的cDNA序列完全一致;重组子用限制性内切酶酶切鉴定,含有正确的IL6-TNF△融合cDNA片段;表达产物经SDS-聚丙烯酰胺凝胶电泳,分子量约为37kD,与预计的相符合;生物学活性分析初步表明,该融合蛋白具有抗肿瘤活性。 相似文献
978.
本工作利用放射性标记的bGH基因(3.0kb)为探针,通过原位杂交定位牛生长激素基因于染色体5q22-26内。该结果与以前的bGH基因定位的结果不同,讨论了基因探针、基因定位方法等方面与定位准确性的关系。 相似文献
979.
Past work on tree-to-tree interactions in semi-arid savannas, through the use of nearest neighbour analysis, has shown both a regular dispersion pattern and a positive correlation between the size of a tree and the distance to its nearest neighbour. From these results, the importance of competition has been inferred. In the present study, tree-to-tree interactions in a mesic savanna woodland at Marondera, Zimbabwe, dominated by relatively small-sized individuals of Brachystegia spiciformis and Julbernardia globiflora, were examined. Results show that the regular dispersion pattern is uncommon; that there are few significant positive correlations between size of individual and distance to nearest neighbour; and that growth rate is not consistently correlated with size and distance of nearest neighbour. Results of a study of regeneration relative to canopy cover suggest a possible mechanism for these findings: unlike semi-arid savannas, young plants often grow in under-canopy environments so that early spacing of young trees does not occur. Significant positive correlations between the size of a tree and the distance to its nearest neighbour were found in a stand of larger, presumably older, trees, suggesting that such a relationship only develops in mesic miombo woodland through a thinning process as the trees mature. Familial clumping, which is common in the study area, may mask spatial patterns.Abbreviations RBAI
Relative basal area increment 相似文献
980.