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51.
Juan Wang Bilian Chen Xiaozhen Rao Jian Huang Min Li 《World journal of microbiology & biotechnology》2007,23(10):1345-1350
The red microalga Porphyridium contains many valuable compounds such as polysaccharides, polyunsaturated fatty acids, and phycoerythrin (PE). In this study,
a uniform design method and regression analysis were used to investigate the effects of initial pH, light intensity, inoculation
ratio, and liquid volume in flask on the optimal biomass, exopolysaccharides (EPS), and PE production of Porphyridium cruentum in a batch culture at laboratory scale. Using regression analysis, we obtained the models to clarify the effects of individual
factors and their interactions on the biomass, EPS, and PE production of P. cruentum. The optimal condition for the biomass was the following: pH 5.0, light intensity 7098.0 lx, inoculation ratio 1:17.2, and
liquid volume 100.0 ml; for EPS was pH 5.0, light intensity 4501.0 lx, inoculation ratio 1:20, and liquid volume 100.3 ml;
while pH 8.0, light intensity 7100.0 lx, inoculation ratio 1:20, and liquid volume 100.3 ml was the best for PE production.
The maximum biomass 3.27 g/l, EPS production 543.1 mg/l, and PE production 132.0 mg/l were demonstrated by confirmatory experiment
to the optimum culture conditions in a reciprocal shaker. The statistical methods used in the present study are useful strategies
for optimizing of culture conditions for other microalgae. 相似文献
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考察了在大肠杆菌中自诱导表达人胰高血糖素样肽-1突变体融合蛋白的可行性,并对自诱导培养条件及培养基成分进行优化,以提高蛋白产量。实验结果表明,最优培养基成分为蛋白胨19.17g/L,酵母膏9.59g/L,Na2HPO45.72g/L,KH2PO45.48g/L,(NH4)2SO42.66g/L,NaCl3.33g/L,甘油2%(V/V),葡萄糖0.68g/L,乳糖6.33g/L,MgSO40.24g/L。在温度33°C、接种量1%、pH7、装瓶量20mL/100mL培养条件下,用该最优培养基自诱导表达人胰高血糖素样肽-1突变体融合蛋白的产量可达348.6mg/L。 相似文献
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Spatial Diversity of Dry Savanna Woodlands 总被引:1,自引:0,他引:1
Friedrich Patrick Graz 《Biodiversity and Conservation》2006,15(4):1143-1157
The dry woodland savannas of Namibia are of significant socio-economic importance. The paper tests the suitability of a number
of diversity indicators developed for species poor systems in Europe in the woodland context. The indicators that were tested
included the species specific mingling index, MSp, the measure of surround and the uniform angle index. The simple application of the methods permit relatively unschooled
crews to conduct an enumeration in the field. The results show that the indicators do not only display current diversity status,
but also reflect the ecological context of the individual species. 相似文献
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