全文获取类型
收费全文 | 7670篇 |
免费 | 506篇 |
国内免费 | 524篇 |
专业分类
8700篇 |
出版年
2024年 | 18篇 |
2023年 | 125篇 |
2022年 | 119篇 |
2021年 | 178篇 |
2020年 | 209篇 |
2019年 | 277篇 |
2018年 | 213篇 |
2017年 | 212篇 |
2016年 | 237篇 |
2015年 | 296篇 |
2014年 | 349篇 |
2013年 | 477篇 |
2012年 | 280篇 |
2011年 | 323篇 |
2010年 | 280篇 |
2009年 | 409篇 |
2008年 | 453篇 |
2007年 | 458篇 |
2006年 | 409篇 |
2005年 | 329篇 |
2004年 | 298篇 |
2003年 | 315篇 |
2002年 | 233篇 |
2001年 | 191篇 |
2000年 | 197篇 |
1999年 | 149篇 |
1998年 | 161篇 |
1997年 | 158篇 |
1996年 | 114篇 |
1995年 | 112篇 |
1994年 | 110篇 |
1993年 | 109篇 |
1992年 | 122篇 |
1991年 | 87篇 |
1990年 | 78篇 |
1989年 | 70篇 |
1988年 | 63篇 |
1987年 | 55篇 |
1986年 | 60篇 |
1985年 | 78篇 |
1984年 | 51篇 |
1983年 | 42篇 |
1982年 | 50篇 |
1981年 | 48篇 |
1980年 | 22篇 |
1979年 | 21篇 |
1978年 | 14篇 |
1977年 | 16篇 |
1976年 | 9篇 |
1972年 | 6篇 |
排序方式: 共有8700条查询结果,搜索用时 55 毫秒
201.
MEGAN BECKETT FRANCESCO LORETO VIOLETA VELIKOVA CECILIA BRUNETTI MARTINA DI FERDINANDO MASSIMILIANO TATTINI CARLO CALFAPIETRA JILL M. FARRANT 《Plant, cell & environment》2012,35(12):2061-2074
We investigated the photosynthetic limitations occurring during dehydration and rehydration of Xerophyta humilis, a poikilochlorophyllous resurrection plant, and whether volatile and non‐volatile isoprenoids might be involved in desiccation tolerance. Photosynthesis declined rapidly after dehydration below 85% relative water content (RWC). Raising intercellular CO2 concentrations during desiccation suggest that the main photosynthetic limitation was photochemical, affecting energy‐dependent RuBP regeneration. Imaging fluorescence confirmed that both the number of photosystem II (PSII) functional reaction centres and their efficiency were impaired under progressive dehydration, and revealed the occurrence of heterogeneous photosynthesis during desiccation, being the basal leaf area more resistant to the stress. Full recovery in photosynthetic parameters occurred on rehydration, confirming that photosynthetic limitations were fully reversible and that no permanent damage occurred. During desiccation, zeaxanthin and lutein increased only when photosynthesis had ceased, implying that these isoprenoids do not directly scavenge reactive oxygen species, but rather protect photosynthetic membranes from damage and consequent denaturation. X. humilis was found to emit isoprene, a volatile isoprenoid that acts as a membrane strengthener in plants. Isoprene emission was stimulated by drought and peaked at 80% RWC. We surmise that isoprene and non‐volatile isoprenoids cooperate in reducing membrane damage in X. humilis, isoprene being effective when desiccation is moderate while non‐volatile isoprenoids operate when water deficit is more extreme. 相似文献
202.
在蔗糖预培养诱导耐脱水性过程中黄皮胚轴的水分状态和可溶性蛋白含量的变化 总被引:5,自引:0,他引:5
逐步增加培养基的蔗糖浓度可提高黄皮胚轴的耐脱水性。用差示扫描量热仪(DSC)和SDS—PAGE方法探讨了黄皮胚轴在耐脱水性获得过程中的水分状态和可溶性蛋白的变化。DSC图谱显示,蔗糖预培养胚轴的升温图谱与对照不同,有比较明显的台阶式变化,有玻璃化形成的倾向;应用线性回归方程法或冰熔化热法计算黄皮胚轴的不可冻结水,发现蔗糖预培养胚轴的不可冻结水含量与对照胚轴无显著差异。蛋白分析表明,蔗糖预培养诱导黄皮胚轴中可溶性蛋白增加68%,其中尤以20kD蛋白的增幅最大。 相似文献
203.
Magdalena Kanabus Adrianna Nowicka Ewa Sledziewska-Gójska Piotr Jonczyk Zygmunt Ciesla 《Molecular & general genetics : MGG》1995,247(2):216-221
It has previously been suggested that inhibition of the proofreading 3-5 exonuclease activity of DNA polymerase may play an important role in generation of UV-induced mutations inEscherichia coli. Our previous work showing that overproduction of , the proofreading subunit of DNA polymerase III, counteracts the SOS mutagenic response ofE. coli seemed to be consistent with this hypothesis. To explore further the nature of the antimutagenic effect of we constructed plasmid pMK17, which encodes only two of the three highly conserved segments of — Exol and ExoII; the third segment, ExoIII, which is essential for 3–5 exonuclease activity, is deleted. We show that at 40°C, over-production of the truncated e subunit significantly delays production of M13 phage, suggesting that the protein retains its capacity to bind to DNA. On the other hand, the presence of pMK17 in atrpE65 strain growing at 40°C causes a 10-fold decrease in the frequency of UV-induced Trp+ mutations. This antimutagenic effect of the truncated s is effectively relieved by excess UmuD,C proteins. We also show that the presence of plasmid pIP21, which contains thednaQ49 allele encoding an subunit that is defective in proofreading activity, almost completely prevents generation of UV-induced mutations in thetrpE65 strain. We propose that the DNA binding ability of free , rather than its 3–5 exonuclease activity, affects processing of premutagenic UV-induced lesions, possibly by interfering with the interaction between the UmuC-UmuD-RecA complex and Pol III holoenzyme. This interaction is probably a necessary condition for translesion synthesis. 相似文献
204.
Satiation-dependent, intra-cohort variations in prey size selection of young roach (Rutilus rutilus)
The tolerance to freezing and thawing of Leucodon sciuroides, a moss growing in mountainous areas of the Mediterranean (south-east Spain), was investigated by means of CO2 gas exchange, modulated chlorophyll (Chl) a fluorescence and pigment analysis by high-performance liquid chromatography. Evidence is presented for freezing-induced decreases
in CO2 fixation that enhance non-radiative dissipation of absorbed light energy, a process which protects the photosynthetic apparatus.
The photosynthetic apparatus of L. sciuroides remained fully recuperable after freezing, as indicated by the recovery of photosynthetic CO2 fixation and Chl fluorescence parameters to pre-freezing values during thawing. The rapid recovery of photosynthesis activity
during thawing in L. sciuroides suggests that this moss is capable of tolerating freeze-thaw cycles in a manner similar to that found at higher latitudes
or in the Antarctic. The resistance of the photosynthetic apparatus of this moss to freezing might be achieved, at least partially,
through the employment of dissipative pathways, such as non-radiative dissipation of absorbed light energy.
Received: 4 June 1998 / Accepted: 15 February 1999 相似文献
205.
全球范围内干旱频率和强度的增加严重影响树木生长,甚至导致森林大面积死亡。压力-容积(PV)曲线能够反映树木对干旱的容忍能力,但在局域尺度上尚未确定哪个PV曲线参数具有最优指示性。通过测定东北温带森林20种主要树种(包括16种被子植物和4种裸子植物)的PV曲线性状,包括质壁分离时的相对含水量(RWCtlp)、失膨点叶水势(TLP)、饱和含水时的叶渗透势(π0)、细胞弹性模量(ε)、叶水容(Cleaf)及叶结构性状(比叶面积和叶密度),研究局域尺度上叶片耐旱性的最佳指示性状,并分析叶片PV性状与结构性状间的相关性。结果表明: 被子植物的RWCtlp 显著大于裸子植物,但其Cleaf 显著小于裸子植物,这表明用RWCtlp和Cleaf可以指示东北温带森林不同功能型树种间耐旱性的大小。在被子植物中,TLP和π0与叶密度呈显著负相关,且均与比叶面积呈显著正相关;而ε与比叶面积呈显著负相关。然而,裸子植物PV曲线性状与叶结构性状之间呈现与被子植物完全相反的趋势。裸子植物与被子植物树种之间PV曲线性状与叶结构性状关系的差异,可能归因于二者采取不同的干旱响应和适应策略。 相似文献
206.
The physiological basis of plant reaction to and tolerance of aluminium (Al) is poorly understood. We review the results of
investigations into Al toxicity and root physiology to develop a theoretical basis for explaining the reaction of the root
to Al, including suggested roles for Ca2+, mucilaginous cap secretions and endogenous growth regulators in mediating a transmitted response between Al-damaged cap
cells and the interacting cell populations of the cap and root.
This information is used to identify possible mechanisms of Al tolerance, notably involving signal transduction, Al uptake
pathways and root morphogenesis; and to briefly discuss how procedures selecting for Al tolerance may be improved by incorporating
the concept of stimulus-response coupling.
Similarities in the responses of roots to Al and other signals (e.g. gravity, light, mechanical impedance) are used to develop the hypothesis that roots respond to environmental signals by way
of a common regulatory system. New research prospects for extending our perception of Al tolerance mechanisms are identified. 相似文献
207.
Jessica Carrington Mads Kuhlmann Andersen Kaylen Brzezinski Heath A. MacMillan 《Proceedings. Biological sciences / The Royal Society》2020,287(1941)
There is a growing appreciation that insect distribution and abundance are associated with the limits of thermal tolerance, but the physiology underlying thermal tolerance remains poorly understood. Many insects, like the migratory locust (Locusta migratoria), suffer a loss of ion and water balance leading to hyperkalaemia (high extracellular [K+]) in the cold that indirectly causes cell death. Cells can die in several ways under stress, and how they die is of critical importance to identifying and understanding the nature of thermal adaptation. Whether apoptotic or necrotic cell death pathways are responsible for low-temperature injury is unclear. Here, we use a caspase-3 specific assay to indirectly quantify apoptotic cell death in three locust tissues (muscle, nerves and midgut) following prolonged chilling and recovery from an injury-inducing cold exposure. Furthermore, we obtain matching measurements of injury, extracellular [K+] and muscle caspase-3 activity in individual locusts to gain further insight into the mechanistic nature of chilling injury. We found a significant increase in muscle caspase-3 activity, but no such increase was observed in either nervous or gut tissue from the same animals, suggesting that chill injury primarily relates to muscle cell death. Levels of chilling injury measured at the whole animal level, however, were strongly correlated with the degree of haemolymph hyperkalaemia, and not apoptosis. These results support the notion that cold-induced ion balance disruption triggers cell death but also that apoptosis is not the main form of cell damage driving low-temperature injury. 相似文献
208.
Using microcosm experiments, we investigated the interactive effects of temperature and light on specific growth rates of three species each of the phytoplanktonic genera Cryptomonas and Dinobryon. Several species of these genera play important roles in the food web of lakes and seem to be sensitive to high water temperature. We measured growth rates at three to four photon flux densities ranging from 10 to 240 μmol photon · m?2 · s?1 and at 4–5 temperatures ranging from 10°C to 28°C. The temperature × light interaction was generally strong, species specific, and also genus specific. Five of the six species studied tolerated 25°C when light availability was high; however, low light reduced tolerance of high temperatures. Growth rates of all six species were unaffected by temperature in the 10°C–15°C range at light levels ≤50 μmol photon · m?2 · s?1. At high light, growth rates of Cryptomonas spp. increased with temperature until the temperature optimum was reached and then declined. The Dinobryon species were less sensitive than Cryptomonas spp. to photon flux densities of 40 μmol photon · m?2 · s?1 and 200 μmol photon · m?2 · s?1 over the entire temperature range but did not grow under a combination of very low light (10 μmol photon · m?2 · s?1) and high temperature (≥20°C). Among the three Cryptomonas species, cell volume declined with temperature and the maximum temperature tolerated was negatively related to cell size. Since Cryptomonas is important food for microzooplankton, these trends may affect the pelagic carbon flow if lake warming continues. 相似文献
209.
水稻的光合作用、生长、碳同位素分辨作用及抗渗透胁迫性对CO_2浓度增高的响应(英文) 总被引:3,自引:0,他引:3
四个水稻 (OryzasativaL .)品种“IR72”、“特三矮 2号”、“桂朝 2号”和“Ⅱ优 44 80”在田间栽于含 35 μmol mol和 6 0 μmol molCO2 的塑料大棚中 ,自然光照。高浓度CO2 下供试水稻品种的光合速率变化表现为提高型 (“IR72”、“特三矮 2号”)、稳定型 (“桂朝 2号”的Pn几无变化 )和下调型 (“Ⅱ优 44 80”)。生长速率、穗重、由Δ1 3 C计算而得的长期水分利用效率和清除DPPH· 自由基的能力皆增加。除“Ⅱ优 44 80”外 ,其他 3个品种明显增高总生物量。供试品种的穗重 总生物量比不同程度地受到高浓度CO2 的改变。叶片段经PEG渗透胁迫后 ,不同的生长于高浓度CO2 者的电解质渗漏率较小。结果表明高浓度CO2 可改变水稻的光合作用和水分关系特性 ,品种间不同的响应显示了选育适于未来高浓度CO2 下具有高产和抗逆性品种的可能性。 相似文献
210.
The pseudanthia ofHeliopsis scabra andRudbeckia vulgaris (Asteraceae) were examined during the anthesis for differences in their UV patterns. Distinct changes in the reflectance and absorbance properties could be observed. The results suggest a close correlation between different stages of floral development and pollinator attraction.
Herrn Prof. Dr.Lothar Geitler zum 90. Geburtstag gewidmet. 相似文献