Ontogenetic histology of Stegosaurus plates and spikes

Abstract: The dinosaur Stegosaurus is characterized by osteoderms of alternating plates and terminal paired spikes. Previous studies have described the histological features and possible functions of these osteoderms. However, ontogenetic changes are poorly documented. In this study, the ontogenetic changes of the osteoderms are examined using eight different ontogenetic skeletons (a juvenile, a subadult, a young adult, and five old adults based on the cortical histology of their body skeletons). The juvenile plate and subadult spike show thin cortex and thick cancellous bone. The young adult plates have an extensive vascular network, which is also seen in old adults. Old adult spikes are different from old adult plates in having a thick cortex and a large axial channel. The cortical histology, in both plates and spikes, show well‐vascularized bone tissue consisting of dense mineralized fibres in young adult forms. In old adult forms, the bone tissues in the spikes become more compact and are extensively remodelled. This might contribute to the structural reinforcement of the spikes. The plates in old adult forms also show extensive remodelling and lines of arrested growth, but only limited signs of compaction. The timing for acquisition of features seen in old adults is different between plates (an extensive vascular network in the young adult) and spikes (a thick cortex with a large axial channel in old adults). The result suggests that the timing for plate and spike functions is different. The extensive vascular networks seen in large plates suggest their function is for display and/or thermoregulation. The thick cortical bone of spikes of old adults suggests that spikes acquire a weapon function for defence ontogenetically late.     

Inferring the evolutionary history of divergence despite gene flow in a lizard species,Scincella lateralis (Scincidae), composed of cryptic lineages

Although recent radiations are fruitful for studying the process of speciation, they are difficult to characterize and require the use of multiple loci and analytical methods that account for processes such as gene flow and genetic drift. Using multilocus sequence data, we combine hierarchical cluster analysis, coalescent species tree inference, and isolation‐with‐migration analysis to investigate evolutionary relationships among cryptic lineages of North American ground skinks. We also estimate the extent that gene flow has accompanied or followed diversification, and also attempt to account for and minimize the influence of gene flow when reconstructing relationships. The data best support seven largely parapatric populations that are broadly concordant with mitochondrial (mt)DNA phylogeography throughout most of the species range, although they fail to fully represent extensive mtDNA divergence along the Gulf Coast. Relationships within and among three broad geographical groups are well supported, despite evidence of gene flow among them. Rejection of an allopatric divergence model partially depends on the inclusion of samples from near parapatric boundaries in the analyses, suggesting that allopatric divergence followed by recent migration may best explain migration rate estimates. Accounting for geographical variation in patterns of gene flow can improve estimates of migration–divergence parameters and minimize the influence of contemporary gene flow on phylogenetic inference. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, ?? , ??–??.     

Temperature effects of intraventricular serotonin,norepinephrine and pilocarpine in the morphine-tolerant rat

Studies were undertaken to examine the possibility that changes occur in the responsiveness of thermoregulatory neurons in the anterior hypothalamus to endogenously released neurotransmitters upon the development of tolerance to morphine. In experiments conducted at an environmental temperature of 20–21°C, tolerance produced by the subcutaneous implantation of morphine alkaloid pellets failed to alter the temperature response of rats to intraventricular injections of 5-HT (10 μg), NE (20 μg) or pilocarpine (100 μg). It is concluded that tolerance development to morphine-induced hypothermia is not a result of changes in the postsynaptic sensitivity to the putative neurotransmitters in the thermoregulatory center.     

Exposure to food advertising on television: Associations with children's fast food and soft drink consumption and obesity

There is insufficient research on the direct effects of food advertising on children's diet and diet-related health, particularly in non-experimental settings. We employ a nationally-representative sample from the Early Childhood Longitudinal Survey–Kindergarten Cohort (ECLS-K) and the Nielsen Company data on spot television advertising of cereals, fast food restaurants and soft drinks to children across the top 55 designated-market areas to estimate the relation between exposure to food advertising on television and children's food consumption and body weight. Our results suggest that soft drink and fast food television advertising is associated with increased consumption of soft drinks and fast food among elementary school children (Grade 5). Exposure to 100 incremental TV ads for sugar-sweetened carbonated soft drinks during 2002–2004 was associated with a 9.4% rise in children's consumption of soft drinks in 2004. The same increase in exposure to fast food advertising was associated with a 1.1% rise in children's consumption of fast food. There was no detectable link between advertising exposure and average body weight, but fast food advertising was significantly associated with body mass index for overweight and obese children (≥85th BMI percentile), revealing detectable effects for a vulnerable group of children. Exposure to advertising for calorie-dense nutrient-poor foods may increase overall consumption of unhealthy food categories.     

Proton nmr spectroscopy of high-spin ferrous cytochrome P-450 models

Alkyl mercaptide complexes of both synthetic and natural-derivative iron(II) porphyrins have been characterized in DMSO solution by proton nmr spectroscopy. A single mercaptide ligand binds to form a high-spin iron(II) complex as determined by solution magnetic measurements and the nmr isotropic shift pattern. Ligand exchange is slow on the nmr time scale unlike corresponding 2-methyl imidazole exchange rates which are very rapid. Further comparison of mercaptide and 2-methyl imidazole adducts reveals a downfield bias in isotropic shift values for the mercaptide species, which may be explained by different signs in the dipolar shift term for the two complexes. This apparent magnetic anisotropy of the mercaptide complex is in the same direction, although smaller, than that observed for bacterial cytochrome P-450. Isotropic shift values of at least 250 ppm for methylene resonances of the coordinated mercaptide support a very efficient unpaired spin delocalization for this axial ligand.     

Wildfire effects and post-fire responses of an invasive mesquite population: the interactive importance of grazing and non-native herbaceous species invasion

Aim To determine how responses of an established velvet mesquite (Prosopis velutina Woot.) population to a 2002 wildfire were shaped by grazing and non‐native herbaceous species invasions, both of which influenced fire behaviour. Location The study was conducted on contiguous ranches (one actively grazed by cattle, one that had not been grazed since 1968) in the Sonoita Valley of southern Arizona. Plant communities on both ranches were comprised of Chihuahuan semi‐desert grassland, savanna, and Madrean evergreen woodland ecosystems, but large areas were dominated by Lehmann and Boer lovegrass, African grass species that were introduced more than 50 years ago. Methods We selected 243 individuals that had been defoliated and bark scorched during the fire using a stratified random design based on pre‐fire grazing status and dominant grass cover. After the start of the 2003 growing season, we recorded individual tree characteristics, fire damage, and measures of post‐fire response, and tested for relationships among classes of: grazing status, bark damage, dominant grass cover type, abundance of live and dead aboveground branches, flowering status, and sprout number and size. Analyses of fire damage and post‐fire response were interpreted with respect to values of fireline intensity, scorch height and energy release that were projected by a fire behaviour model, nexus . Results Nearly all of the trees on grazed areas suffered low levels of fire damage, while a majority on ungrazed areas suffered moderate to severe damage. Trees on grazed areas consequently had significantly more leaf‐bearing twigs and branches in 2003 but a very low number of root sprouts, while individuals on ungrazed areas had a greater density of root sprouts but little post‐fire dead branching and almost no living branches. Among the ungrazed grassland types, more than 75% of the trees on Boer lovegrass plots suffered moderate to severe damage, while a similar percentage of trees in native grass areas suffered low damage. These differences were: (1) attributed to variations in fire characteristics that were caused by differences in litter production and removal, and (2) ecologically significant because trees in the severe damage class showed almost no aboveground post‐fire branching, either live or dead in 2003, while trees in the low damage class exhibited a greater amount of both. Main conclusions Our results affirm the notion that effective management of western grasslands where mesquite encroachment has or will become a problem requires a better understanding of how interactions among key ecosystem influences (e.g. fire, grazing, non‐native species) affect not only mesquite seedlings and saplings but also larger, established individuals and thereby the long‐term structure and functioning of semi‐desert grassland ecosystems. As managers shift their focus from eradication to management of mesquite in western grasslands and savannas, our results provide insights into how prescribed fires (and their effects on mesquite populations) differ from wildfires and how such effects may be mediated by the altered land uses and ecosystem characteristics that now exist in many western ecosystems.     

Counter transport of glutamine and choline in cultures of human glioma cells.

Tritiated glutamine and choline were released from human glioma cells in culture when incubated in permeant-free solution. Counter transport experiments revealed that the two neurotransmitter precursors were taken up and released by facilitated diffussion transport systems. It is suggested that glial cells $\text{in}\text{vivo}$ can provide neurons with glutamine and choline for transmitter synthesis via such systems.     

Land use and climate influences on waterbirds in the Prairie Potholes

Aim We examined the influences of regional climate and land‐use variables on mallard (Anas platyrhynchos), blue‐winged teal (Anas discors), ruddy duck (Oxyura jamaicensis) and pied‐billed grebe (Podilymbus podiceps) abundances to inform conservation planning in the Prairie Pothole Region of the United States. Location The US portion of Bird Conservation Region 11 (US‐BCR11, the Prairie Potholes), which encompasses six states within the United States: Montana, North Dakota, South Dakota, Nebraska, Minnesota and Iowa. Methods We used data from the North American Breeding Bird Survey (NABBS), the National Land Cover Data Set, and the National Climatic Data Center to model the effects of environmental variables on waterbird abundance. We evaluated land‐use covariates at three logarithmically related spatial scales (1000, 10,000 and 100,000 ha), and constructed hierarchical spatial count models a priori using information from published habitat associations. Model fitting was performed using a hierarchical modelling approach within a Bayesian framework. Results Models with the same variables expressed at different scales were often in the best model subset, indicating that the influence of spatial scale was small. Both land‐use and climate variables contributed strongly to predicting waterbird abundance in US‐BCR11. The strongest positive influences on waterbird abundance were the percentage of wetland area across all three spatial scales, herbaceous vegetation and precipitation variables. Other variables that we included in our models did not appear to influence waterbirds in this study. Main conclusions Understanding the relationships of waterbird abundance to climate and land use may allow us to make predictions of future distribution and abundance as environmental factors change. Additionally, results from this study can suggest locations where conservation and management efforts should be focused.