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251.
Aditi Pai Igor V. Sharakhov Olga Braginets Christina Costa Guiyun Yan 《Molecular ecology resources》2003,3(3):425-427
We isolated and characterized microsatellite markers for the red flour beetle Tribolium castaneum, an important model species for studies in various areas of evolutionary biology and ecology. A microsatellite‐enriched genomic library was constructed and screened with single stranded oligonucleotide probes [(CCT)17, (AAT)17 and (CAG)17]. Forty‐five primer pairs were designed of which 19 pairs produced successful amplification. Polymorphism screening involved beetles from five beetle strains and revealed 15 polymorphic and four monomorphic markers. The development of polymorphic microsatellite markers will facilitate future ecological and genetic studies involving T. castaneum beetles. 相似文献
252.
253.
Gerhard W. Kalmus William E. Oakley Jr. 《Invertebrate reproduction & development.》2013,57(2-3):205-210
Summary The 5th instar larvae of the common flour beetle, Tribolium confusum, was utilized as an invertebrate model to test the larvicidal potential of oxidizing agents used as flour preservatives. Larvae were exposed to 10 ppm of either diflubenzuron or benzoyl peroxide. Results showed decreases in both total protein and glucose concentrations. Histological examination also indicated smaller deposition of chitinous material in the endocuticle. Both treatments had similar effects. Thus, these data indicate that oxidizing agents mixed in flour may be used for the control of development of flour beetle larvae. 相似文献
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255.
Andreas Berghammer Gregor Bucher Florian Maderspacher M. Klingler 《Development genes and evolution》1999,209(6):382-389
Due to its small size, short life cycle, and easy maintenance, the flour beetle Tribolium castaneum is well suited for the genetic analysis of development. One drawback of Tribolium as a genetic system is, however, the difficulty of keeping embryonic lethal lines. Presently, only few lethal mutations can
be kept as balanced stocks. Therefore, heterozygous carriers must be identified anew in every generation in order to maintain
a recessive embryonic mutation. To alleviate this problem we have devised a block system that allows the simultaneous processing
of many mutant lines or test crosses for visual inspection of larval cuticle phenotypes. Using this technique, one person
can maintain about 100 embryonic lethal stocks, which makes feasible the thorough genetic analysis of embryogenesis in this
species.
Received: 4 November 1998 / Accepted: 7 February 1999 相似文献
256.
Hiromasa Yamauchi Masami Harada Akihiro Miyanoshita 《Bioscience, biotechnology, and biochemistry》2018,82(2):229-231
Tribolium castaneum, a pest of stored grain, has a uniform morphology, preventing the visual identification of strains from different areas. Polymorphisms in the nucleotide sequences of the mitochondrial genes of this species were examined, and combined into seven haplotypes among the test insect specimens originating from Japan, Thailand, and Canada. These results suggested the potential for geographical differentiation. 相似文献
257.
The red flour beetle, Tribolium castaneum, secretes quinones that control the microbial flora in the surrounding environment. These secretions act as an external immune defence that provides protection against pathogens. At high concentrations, however, these secretions are harmful to the host itself, and selection may thus have optimized the level of expression under natural conditions. Here, we show that the expression of external immunity responded to selection during experimental evolution within a few generations. At the same time, one component of internal immune defence (phenoloxidase activity) was compromised in beetles selected for either high or low external defences. Intriguingly, offspring protection against a natural pathogen was reduced in flour obtained from beetle lines selected for low amounts of secretions. Altogether, this suggests that external and internal immune defences work together efficiently under natural conditions, whereas every manipulation on the side of external immune defence comes with costs to the internal immune defence. 相似文献
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259.