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11.
Filipe Anibal Carvalho‐Costa Silvia M Dos Santos Marize Quinhones Pires Catarina Macedo Lopes François Noireau Raquel S. Pacheco 《Journal of vector ecology》2010,35(2):295-300
Chagas disease remains a public health concern in Brazil and other Latin American countries, mainly due to the potential domiciliation of native triatomine species. We analyzed the genetic variability of Triatoma pseudomaculata in sylvatic and peridomestic ecotopes throughout three localities in the northeastern state of Bahia, Brazil. We studied polymorphisms generated by random amplified polymorphic DNA (RAPD) and isoenzyme electrophoresis analyses. Based on RAPD analysis, each specimen was assigned to one of three genetic clusters. Although all sylvatic specimens from one locality were grouped into the same cluster, sylvatic and peridomestic specimens from the other two localities were broadly distributed between the remaining two clusters, suggesting that geographic population structuring was not occurring. Furthermore, isoenzyme analysis suggested that distinct populations were in Hardy‐Weinberg equilibrium. Low statistical values for Wright's Fst index also supported the absence of population structuring and suggested the occurrence of panmixia. We conclude that genetic flow occurs between sylvatic and peridomestic T. pseudomaculata populations, probably as a consequence of passive and active dispersion of the insects, associated with deforestation and anthropic transformations. 相似文献
12.
番茄幼苗对接种晚疫病菌的生理响应 总被引:3,自引:1,他引:2
以番茄晚疫病抗病品系CLN2037E、感病品系5号自交系及其杂种一代F1为材料,人工接种番茄晚疫病菌生理小种T1、T2、T3,研究番茄苗期抵抗晚疫病的生理响应.结果显示:(1)感病品系的膜脂过氧化产物丙二醛(MDA)与过氧化氢(H2O2)的含量增加幅度较大,抗病品系的含量则相对稳定,F1代的变化趋势接近感病品系;(2)抗病品系和感病品系的多酚氧化酶(PPO)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性变化都呈先升高后下降,抗病品系的酶活性高峰出现早且峰值小于感病品系.研究发现,抗病番茄品系幼苗的多项生理指标在接种晚疫病菌后变化幅度小而稳定,并能在短时间内基本恢复到原来的正常状态,从而表现出较强的抗病性. 相似文献
13.
María Sol Gaspe Yael Mariana Provecho Romina Valeria Piccinali Ricardo Esteban Gürtler/ 《Memórias do Instituto Oswaldo Cruz》2015,110(3):310-318
House re-invasion by native triatomines after insecticide-based control campaigns
represents a major threat for Chagas disease vector control. We conducted a
longitudinal intervention study in a rural section (Area III, 407 houses) of Pampa
del Indio, northeastern Argentina, and used wing geometric morphometry to compare
pre-spray and post-spray (re-infestant bugs) Triatoma infestans
populations. The community-wide spraying with pyrethroids reduced the prevalence of
house infestation by T. infestans from 31.9% to < 1% during a
four-year follow-up, unlike our previous studies in the neighbouring Area I. Two
groups of bug collection sites differing in wing shape variables before interventions
(including 221 adults from 11 domiciles) were used as a reference for assigning 44
post-spray adults. Wing shape variables from post-spray, high-density bug colonies
and pre-spray groups were significantly different, suggesting that re-infestant
insects had an external origin. Insects from one house differed strongly in wing
shape variables from all other specimens. A further comparison between insects from
both areas supported the existence of independent re-infestation processes within the
same district. These results point to local heterogeneities in house re-infestation
dynamics and emphasise the need to expand the geographic coverage of vector
surveillance and control operations to the affected region. 相似文献
14.
Jean-Pierre Dujardin Truong Xuan Lam Pham Thi Khoa Christopher John Schofield 《Memórias do Instituto Oswaldo Cruz》2015,110(3):319-323
The migration of invasive vector species has contributed to the worldwide extension
of infectious diseases such as dengue (Aedes aegypti) and chikungunya (Aedes
albopictus). It is probably a similar behaviour for certain vectors of Chagas disease
which allowed it to become a continental burden in Latin America. One of them,
Triatoma rubrofasciata has also been spreading throughout the tropical and
subtropical world. Here, the recent and massive peridomestic presence of T.
rubrofasciata in Vietnam cities is reported, and tentatively explained, highlighting
the need for improved entomological surveillance. 相似文献
15.
Paula Medone Soledad Ceccarelli Paul E. Parham Andreína Figuera Jorge E. Rabinovich 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2015,370(1665)
Chagas disease, caused by the parasite Trypanosoma cruzi, is the most important vector-borne disease in Latin America. The vectors are insects belonging to the Triatominae (Hemiptera, Reduviidae), and are widely distributed in the Americas. Here, we assess the implications of climatic projections for 2050 on the geographical footprint of two of the main Chagas disease vectors: Rhodnius prolixus (tropical species) and Triatoma infestans (temperate species). We estimated the epidemiological implications of current to future transitions in the climatic niche in terms of changes in the force of infection (FOI) on the rural population of two countries: Venezuela (tropical) and Argentina (temperate). The climatic projections for 2050 showed heterogeneous impact on the climatic niches of both vector species, with a decreasing trend of suitability of areas that are currently at high-to-moderate transmission risk. Consequently, climatic projections affected differently the FOI for Chagas disease in Venezuela and Argentina. Despite the heterogeneous results, our main conclusions point out a decreasing trend in the number of new cases of Tr. cruzi human infections per year between current and future conditions using a climatic niche approach. 相似文献
16.
Infestation of arboreal nests of coatis by triatomine species,vectors of Trypanosoma cruzi,in a large Neotropical wetland
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Juliane Saab de Lima Fabiana Lopes Rocha Fernanda Moreira Alves Elias Seixas Lorosa Ana Maria Jansen Guilherme de Miranda Mourão 《Journal of vector ecology》2015,40(2):379-385
The coati (Nasua nasua, Carnivora) is a medium‐sized mammal common in the Pantanal of Brazil. Unlike most mammals, coatis construct arboreal nests used for resting and reproduction. In this region, the coati is an important host of Trypanosoma cruzi, the causative agent of Chagas disease. There are two possible routes through coatis can be infected by T. cruzi: the oral route or the vectorial route. However, the relative importance of each of these routes in the infection of coatis and its role in the sylvatic cycle of the parasite are unknown. Our objectives were to investigate: (i) whether coati nests were infested by triatomine bugs, (ii) what species were frequent in the nests, (iii) whether the triatomines in nests were infected by T. cruzi, and (iv) what were the food resources of these triatomines. Eight of the 24 nests sampled were infested with triatomines, a total of 37 specimens of at least two species (Rhodnius stali and Triatoma sordida). In one nest, R. stali and T. sordida co‐occurred and both fed on multiple resources, including coatis. This is the first report of triatomines occurring in arboreal nests of coatis. The co‐occurrence of two different genera of triatomine vectors and coatis within the limited space of the coati nests provide multiple opportunities for the exchange of the protozoan parasite through both the vectorial and oral transmission routes. 相似文献
17.
Tolga O. Bozkurt Khaoula Belhaj Yasin F. Dagdas Angela Chaparro‐Garcia Chih‐Hang Wu Liliana M. Cano Sophien Kamoun 《Traffic (Copenhagen, Denmark)》2015,16(2):204-226
A number of plant pathogenic and symbiotic microbes produce specialized cellular structures that invade host cells where they remain enveloped by host‐derived membranes. The mechanisms underlying the biogenesis and functions of host–microbe interfaces are poorly understood. Here, we show that plant late endocytic trafficking is diverted toward the extrahaustorial membrane (EHM); a host–pathogen interface that develops in plant cells invaded by Irish potato famine pathogen Phytophthora infestans. A late endosome and tonoplast marker protein Rab7 GTPase RabG3c, but not a tonoplast‐localized sucrose transporter, is recruited to the EHM, suggesting specific rerouting of vacuole‐targeted late endosomes to a host–pathogen interface. We revealed the dynamic nature of this process by showing that, upon activation, a cell surface immune receptor traffics toward the haustorial interface. Our work provides insight into the biogenesis of the EHM and reveals dynamic processes that recruit membrane compartments and immune receptors to this host–pathogen interface. 相似文献
18.
Changes of Genotype,Sensitivity and Aggressiveness in Phytophthora infestans Isolates Collected in European Countries in 1997, 2006 and 2007 总被引:1,自引:0,他引:1
Ulrich Gisi Florian Walder Zipora Resheat‐Eini Dominique Edel Helge Sierotzki 《Journal of Phytopathology》2011,159(4):223-232
A total of 241 isolates of Phytophthora infestans were collected in 1997, 2006 and 2007 in eight European countries and characterized with molecular markers (simple sequence repeats, SSR genotypes) and phenotypic traits such as sensitivity to fungicides, mating type and aggressiveness. The mating type distribution changed from mainly A1 in 1997 to a majority of A2 in 2007. No resistant isolates were detected for fluazinam and mandipropamid, whereas the proportion of isolates resistant to mefenoxam (MFX) was high and increased over the years. There was no genetic link between mating type and MFX resistance. Aggressiveness (product between lesion expansion and sporulation capacity) was slightly higher for MFX‐resistant compared to sensitive isolates and for isolates collected later compared to earlier in the same season. It was about equally high for A1 and A2 types, and for French isolates in 1997 and British isolates in 2007, but lower for French isolates in 2007. Six different SSR genotype families were distinguished. In 1997, populations were dominated by genotype families I and III/IV, which significantly declined in 2007 being largely displaced by genotype families II (‘blue 13’ type) and V, which are by coincidence mainly A2 MFX resistant and A1 MFX sensitive, respectively. However, mating type and MFX resistance were genetically not linked to SSR genotypes. 相似文献
19.
A survey for natural entomopathogenic fungi of the Chagas disease vector Triatoma infestans was conducted in five provinces of Argentina since 2001. Nymphs (1.5%) and adults (3.3%) infected with a strain of the fungus Beauveria bassiana were found at Dean Funes, Córdoba province, Argentina. Field collected insects that died in the laboratory were maintained in moist chambers and incubated at 22 °C. Beauveria bassiana from infected insects was cultured on SDAY media. Pathogenicity tests were conducted with a conidial suspension (1 × 107 conidia/ml) of this isolate on T. infestans adults. A mortality rate of 100% was obtained at 15 days post-infection. This is the first record of natural infection of T. infestans by B. bassiana. 相似文献
20.
Liu Z Bos JI Armstrong M Whisson SC da Cunha L Torto-Alalibo T Win J Avrova AO Wright F Birch PR Kamoun S 《Molecular biology and evolution》2005,22(3):659-672
Phytophthora infestans, the organism responsible for the Irish famine, causes late blight, a re-emerging disease of potato and tomato. Little is known about the molecular evolution of P. infestans genes. To identify candidate effector genes (virulence or avirulence genes) that may have co-evolved with the host, we mined expressed sequence tag (EST) data from infection stages of P. infestans for secreted and potentially polymorphic genes. This led to the identification of scr74, a gene that encodes a predicted 74-amino acid secreted cysteine-rich protein with similarity to the Phytophthora cactorum phytotoxin PcF. The expression of scr74 was upregulated approximately 60-fold 2 to 4 days after inoculation of tomato and was also significantly induced during early stages of colonization of potato. The scr74 gene was found to belong to a highly polymorphic gene family within P. infestans with 21 different sequences identified. Using the approximate and maximum likelihood (ML) methods, we found that diversifying selection likely caused the extensive polymorphism observed within the scr74 gene family. Pairwise comparisons of 17 scr74 sequences revealed elevated ratios of nonsynonymous to synonymous nucleotide-substitution rates, particularly in the mature region of the proteins. Using ML, all 21 polymorphic amino acid sites were identified to be under diversifying selection. Of these 21 amino acids, 19 are located in the mature protein region, suggesting that selection may have acted on the functional portions of the proteins. Further investigation of gene copy number and organization revealed that the scr74 gene family comprises at least three copies located in a region of no more than 300 kb of the P. infestans genome. We found evidence that recombination contributed to sequence divergence within at least one gene locus. These results led us to propose an evolutionary model that involves gene duplication and recombination, followed by functional divergence of scr74 genes. This study provides support for using diversifying selection as a criterion for identifying candidate effector genes from sequence databases. 相似文献