石灰岩山地优势种淡竹生物量分配的影响主因研究

该研究在江西省瑞昌市设置9个淡竹林样地,调查和测定了淡竹林密度、淡竹各构件的生物量和总生物量,以及土壤含水率、土层厚度、林下裸岩率、pH、电导率、全氮和全磷等7个土壤环境因子,并对竹林密度、土壤环境因子和淡竹生物量分配指标进行了相关分析和回归分析。结果表明：(1)密度与淡竹蔸比重相关系数r达0.66( P＝0.02<0.05),而与叶比重、枝比重、秆比重、鞭比重、根比重及根冠比均无显著相关关系;土壤环境因子与生物量分配指标有密切相关,环境主成分Z1与叶比重、秆比重及蔸比重均显著相关(P<0.05),Z2与鞭比重显著相关(P＝0.034<0.05)。(2)密度与土壤环境因子密切相关(P<0.05),控制土壤环境因子的偏相关分析显示密度与淡竹生物量分配不显著相关( P>0.05),而控制密度时,土壤环境因子与淡竹生物量分配仍有显著相关关系(P<0.05);逐步回归分析也验证了偏相关分析的结果,密度被排除出回归方程。分析认为,土壤含水率、土层厚度及土壤养分等环境因子是影响石灰岩山地优势种淡竹生物量分配的主因,密度对生物量分配的影响实为土壤环境因子的间接作用。该研究结果为石灰岩地区植被恢复提供了理论支撑。     

Serum B-cell activating factor predicts prognosis in nasal type,extranodal NK/T cell lymphoma

B-cell activating factor (BAFF) plays important roles in a variety of lymphoid malignancies. Compared with healthy adults, patients with non-Hodgkin lymphoma had higher level of serum BAFF, and it corresponded with disease severity, response for therapy and clinical outcome. Latent membrane protein 1 (LMP1) encoded by Epstein-Barr virus (EBV) which is a known agent of nasal, extranodal NK/T cell lymphoma (ENKTCL) can switch the BAFF activating promoter leading to higher expression of BAFF in EBV-related tumor cells. However, the relationship between BAFF and ENKTCL has not been reported. Here we proposed a hypothesis that BAFF might play a regulatory role in ENKTCL development and maintenance. Our results showed that serum BAFF in ENKTCL patients was significantly higher than that in control group and negatively correlated with patients’ survival. It may be a valuable prognostic factor and deserved further study.     

Association between high expression macrophage migration inhibitory factor (MIF) alleles and West Nile virus encephalitis

Infection with mosquito-borne West Nile virus (WNV) is usually asymptomatic but can lead to severe WNV encephalitis. The innate cytokine, macrophage migration inhibitory factor (MIF), is elevated in patients with WNV encephalitis and promotes viral neuroinvasion and mortality in animal models. In a case-control study, we examined functional polymorphisms in the MIF locus in a cohort of 454 North American patients with neuroinvasive WNV disease and found patients homozygous for high-expression MIF alleles to be >20-fold (p = 0.008) more likely to have WNV encephalitis. These data indicate that MIF is an important determinant of severity of WNV neuropathogenesis and may be a therapeutic target.     

Plasma levels of TGF-β1 in homeostasis of the inflammation in sickle cell disease

Sickle cell disease (SCD) represents a chronic inflammatory condition with complications triggered by the polymerization of hemoglobin S (Hb S), resulting in a series of cellular interactions mediated by inflammatory cytokines, as the transforming growth factor beta (TGF-β), which plays an important role in inflammation resolution. This study assessed the relation between SCD inflammation and the plasma concentration of TGF-β1, and also checked the influence of the presence of −509C/T polymorphism in TGFB1 gene on TGF-β1 plasma values. The plasma levels of TGF-β1 were quantified by ELISA in 115 patients with SCD (genotypes SS, SD-Los Angeles, Sβ-thalassemia and SC) and in 58 individuals with no hemoglobinopathies (Hb AA), as the control group. The −509C/T polymorphism in TGFB1 gene was screened by PCR-RFLP. The correlation between TGF-β1 plasma levels and the inflammation was based on its association with the count of platelets, total white blood cells (WBC) and neutrophils in the peripheral blood. Patients with SCD showed plasma levels of TGF-β1 higher than the control group, especially the Hb SS genotype, followed by the group with Hb SD. Polymorphism investigation showed no interference in the values obtained for the cytokine in the groups evaluated. All SCD groups showed TGF-β1 levels positively correlated to the platelets and WBC counts. The original data obtained in this study for SCD support the involvement of TGF-β1 in regulating of the inflammatory response and suggest that this marker possibly may become a potential therapeutic target in the treatment of the disease.     

TL1A increased IL-6 production on fibroblast-like synoviocytes by preferentially activating TNF receptor 2 in rheumatoid arthritis

TNF-like protein 1A (TL1A), a member of tumor necrosis factor family, recognized as a ligand of death receptor 3 (DR3) and decoy receptor 3 (DcR3). The interaction of TL1A and DR3 may participate in the pathogenesis of some autoimmune diseases including rheumatoid arthritis (RA). Our previous results showed that high concentrations of TL1A could be found in synovial and serum in RA patients, and it was correlated with disease severity. In addition, TL1A could promote Th17 differentiation induced by TGF-β and IL-6 and increased the production of IL-17A. In the present study, we found that TL1A could promote the expression of IL-6 on fibroblast-like synoviocytes (FLS) of RA patients via NF-κB and JNK signaling pathway. TL1A-stimulated FLS increased the percentage of Th17 of peripheral blood mononuclear cells (PBMC) in RA via the production of IL-6, a critical cytokine involved in the differentiation of Th17. Moreover, the blocking of tumor necrosis factor receptor 2 (TNFR2) decreased TL1A-stimulated IL-6 production by RA FLS. Our results suggest that TL1A was capable of acting on RA FLS to elevate IL-6 expression, which promoted the production of Th17. More importantly, we showed that TL1A could influence RA FLS through binding to TNFR2 rather than DR3 on FLS, which indicated that the treatment of TNF inhibitors not only blocked the TNF but also suppressed the TL1A in RA patients.     

青岛胶州湾四种类型湿地AM真菌多样性

对青岛胶州湾4种类型湿地（盐田、滩涂、湖泊和河口）中芦苇Phragmites communis、香蒲Typha orientalis和碱蓬Suaeda glauca 根围土壤中丛枝菌根（arbuscular mycorrhizal,AM）真菌进行孢子分离与鉴定,分析湿地生态系统中植物根围AM真菌群落特征。共分离到AM真菌5属10种,包括斗管囊霉属Funneliformis 2种、无梗囊霉属Acaulospora 3种、近明球囊霉属Claroideoglomus 2种、球囊霉属Glomus 1种、巨孢囊霉属Gigaspora 2种,其中,斗管囊霉属Funneliformis及地斗管囊霉Funneliformis geosporum的分离频度和相对多度最高,分别为湿地中AM真菌优势属和优势种。滩涂和河口湿地中植物AM真菌侵染率显著高于湖泊和盐田湿地植物,AM真菌孢子密度以滩涂湿地最高（572个/20mL）,湖泊湿地最低（220个/20mL）;滩涂湿地的种丰度和Shannon-Wiener指数最高,分别为3.8和1.2。从植物种类来看,AM真菌侵染率总体呈现出香蒲>碱蓬>芦苇,AM真菌孢子密度以香蒲最高,芦苇最低,植物种类对AM真菌种丰度和Shannon-Wiener指数影响不显著（P>0.05）。二因素方差分析和典型RDA相关分析表明,寄主植物种类对AM真菌孢子密度有一定影响,但湿地类型对AM真菌多样性的影响更为显著（P<0.05）,胶州湾湿地土壤因子Ca ²⁺、速效P含量与AM真菌孢子密度、物种丰度和多样性指数显著负相关,而速效K、Na ⁺、pH与其显著正相关。结果表明植物种类主要影响AM真菌孢子密度,AM真菌多样性受植物种类和湿地类型综合影响,滩涂湿地较丰富的AM真菌多样性可能与该湿地较良好的理化性质有关。     

脆木耳生物学特性及驯化栽培

对采自海南省白沙县鹦哥岭阔叶树腐木上的标本,根据形态学特征和多基因分析,鉴定为脆木耳Auricularia fibrillifera,经分离纯化获得纯菌株作为实验材料,为了充分利用和开发木耳属资源,首次对该种的生物学特性和驯化栽培进行了研究。研究不同碳源、氮源、无机盐和生长因子在固体培养条件下对脆木耳菌丝生长的影响,对以上4个因素进行单因素试验,从中选出3个最优的水平进行正交试验。结果表明：脆木耳菌丝最适生长温度为33℃,最佳碳源为麦芽糖,最佳氮源为牛肉粉,最佳无机盐添加量为1.5% PO₄^3--1% Mg²⁺,最佳生长因子为玉米汁。驯化栽培过程中,栽培配方为：木屑78%,麸皮20%,石膏粉1%,石灰粉1%,发菌温度为22℃可使脆木耳菌丝在40d左右满袋,且生长旺盛,可培育出子实体。     

沙丁胺醇联合福多司坦治疗慢性阻塞性肺疾病稳定期的疗效及对患者血清IL-6、TNF-α、hs-CRP水平的影响

目的:探讨沙丁胺醇联合福多司坦治疗慢性阻塞性肺疾病稳定期的临床疗效及对患者血清白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、超敏c反应蛋白(hs-CRP)水平的影响。方法:选择2016年1月到2017年1月我院接诊的稳定期慢性阻塞性肺病患者100例作为研究对象,按照随机数表法分为观察组(n=51)和对照组(n=49)。对照组使用沙丁胺醇治疗,观察组采用沙丁胺醇联合福多司坦治疗。比较两组治疗后的疗效、治疗前后血清IL-6、TNF-α、hs-CRP、肺功能的变化及不良反应的发生情况。结果:治疗后,观察组临床疗效总有效率(94.12%)显著高于对照组(75.51%,P0.05)。两组患者治疗后血清IL-6、TNF-α、hs-CRP水平均治疗前均明显下降,且观察组患者血清IL-6、TNF-α、hs-CRP水平均明显低于对照组(P0.05);两组治疗后各第1秒用力呼气容积(FEV1)、用力肺活量(FVC)、最大呼气流量(PEF)较治疗前均显著升高(P0.05),且观察组FEV1、FVC、PEF均明显高于对照组(P0.05);两组患者不良反应总发生率分别19.61%、38.78%,观察组显著低于对照组(P0.05)。结论:沙丁胺醇联合福多司坦治疗慢性阻塞性肺疾病稳定期的临床疗效和安全性均显著优于单用沙丁胺醇治疗,可能与其有效改善患者血清IL-6、TNF-α、hs-CRP水平有关。     

成纤维细胞生长因子21对非酒精性脂肪性肝炎的作用及机制的研究

目的:探索成纤维细胞生长因子21(FGF-21)对非酒精性脂肪性肝炎(NASH)的作用及其机制。方法:用浓度为500μmol/L的油酸和棕榈酸混合物(摩尔比=2:1)诱导HepG2细胞建立NASH细胞模型,实验分为5组:正常对照组(Control)、模型组(Model)、低剂量FGF-21组(LFGF-21,0.5μmol/L)、中剂量FGF-21组(MFGF-21,1.0μmol/L)和高剂量FGF-21组(HFGF-21,2.0μmol/L),油红O染色法观察细胞内脂滴,全自动生化分析仪检测细胞内ALT、AST、TC、TG的水平,Real-time PCR和Western blot分别检测细胞内核因子E2相关因子-2(Nrf-2)、核苷酸结合寡聚化结构域样受体3(NLRP3)的m RNA和蛋白水平,ELISA检测IL-1β、TNF-α水平。结果:NASH细胞造模成功,油红O染色结果显示对照组细胞无明显脂滴蓄积,模型组细胞内可见大量橘红色脂滴,并出现融合现象。不同浓度FGF-21治疗组的细胞内红色脂滴明显减少,并呈剂量依赖性。模型组ALT、AST、TC、TG、IL-1β、TNF-α和NLRP3的水平均高于对照组(P0.05),FGF-21治疗组其水平低于模型组(P0.05)。模型组Nrf2的水平低于对照组(P0.05),FGF-21治疗组Nrf2的水平高于模型组(P0.05)。结论:FGF-21通过促进Nrf-2、抑制NLRP3减少非酒精性脂肪性肝炎细胞的脂质沉积,减轻炎症反应,对NASH有保护作用。     

乳腺癌患者心理韧性相关影响因素分析及心理韧性与焦虑的关系

目的:分析乳腺癌患者心理韧性的影响因素,并分析心理韧性与焦虑的关系。方法:于2017年2月～2018年4月期间,选择蚌埠医学院第二附属医院收治的乳腺癌患者287例为研究对象,分别采用心理韧性量表(RS)、焦虑自评量表(SAS)评估研究对象的心理韧性及焦虑状态,并采用多因素Logistic回归分析法分析乳腺癌患者心理韧性相关的影响因素,应用多元逐步线性回归分析法分析乳腺癌患者心理韧性与焦虑的关系。结果:乳腺癌患者RS得分为(76.72±9.82)分。单因素分析显示,不同年龄、居住地、家庭月收入及费用支出形式患者的RS得分比较差异有统计学意义(P0.05);不同文化程度、婚姻状况、手术类型及肿瘤分期患者的RS得分比较差异无统计学意义(P0.05)。多因素Logistic回归分析结果显示,年龄为20～40岁、居住地为农村、家庭月收入3000元、费用支出形式为自费是乳腺癌患者心理韧性的影响因素(P0.05)。乳腺癌患者SAS得分为(5.02±1.42)分,以心理韧性为自变量,焦虑为因变量进行多元逐步线性回归分析,结果显示,乳腺癌患者心理韧性为焦虑的预测因子,其心理韧性水平越高,焦虑程度越低(P0.05)。结论:乳腺癌患者心理韧性水平偏低,且其对患者焦虑状态具有预测作用,年龄为20～40岁、居住地为农村、家庭月收入3000元、费用支出形式为自费是乳腺癌患者心理韧性的影响因素,临床治疗中应该根据以上影响因素进行相关干预。