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101.
Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine has been known for a long time to prevent tuberculosis (TB) worldwide since 1921. Nonetheless, we know little about BCG membrane proteome. In the present study, we utilized alkaline incubation and Triton X-114-based methods to enrich BCG membrane proteins and subsequently digested them using proteolytic enzyme. The recovered peptides were further separated by 2-D LC and identified by ESI-MS/MS. As a result, total 474 proteins were identified, including 78 integral membrane proteins (IMPs). Notably, 18 BCG IMPs were described for the first time in mycobacterium. Further analysis of the 78 IMPs indicated that the theoretical molecular mass distribution of them ranged from 8.06 to 167.86 kDa and pI scores ranged from 4.40 to 11.60. Functional classification revealed that a large proportion of the identified IMPs (67.9%, 53 out of 78) were involved in cell wall and cell processes functional group. In conclusion, here we reported a comprehensive profile of the BCG membrane subproteome. The present investigation may allow the identification of some valuable vaccine and drug target candidates and thus provide basement for future designing of preventive, diagnostic, and therapeutic strategies against TB. 相似文献
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103.
Piotr Indykiewicz Patrycja Podlaszczuk Maciej Kamiski Radosaw Wodarczyk Piotr Minias 《Ibis》2019,161(4):744-758
Central nesting sites within avian colonies are often more profitable in terms of fitness, as they can offer better protection against predators compared with colony edges. Thus, central sites are expected to attract high‐quality individuals, which should produce a clear central–periphery gradient in the phenotypic quality of nesting individuals and reproductive output within colonies. The aim of this study was to assess spatial patterns of individual quality within a colony of a common larid, the Black‐headed Gull Chroicocephalus ridibundus. We found that laying was advanced in the central zone of the colony when compared with the periphery, and central pairs tended to lay larger eggs and had higher hatching success than peripheral pairs. Centrally nesting individuals had higher blood haemoglobin concentrations, size‐corrected body mass and heterozygosity (males only), indicating their better condition and higher genetic quality. In contrast, central females had lower plasma albumin concentration, which could possibly be attributed to their greater investment in egg production. Finally, pairs breeding in the central zone of the colony had an elevated heterophil/lymphocyte ratio, suggesting that social stress can be recognized as an important cost of nesting in dense aggregations. The results provide empirical support for central–periphery gradients in the individual quality of colonially nesting Black‐headed Gulls, indicating that spatial gradients in pair quality should be carefully taken into account when conducting research on colonial birds. 相似文献
104.
In angiosperms, cyclic electron transport (CET) around photosystem I (PSI) consists of two pathways, depending on PGR5/PGRL1 proteins and the chloroplast NDH complex. In single mutants defective in chloroplast NDH, photosynthetic electron transport is only slightly affected at low light intensity, but in double mutants impaired in both CET pathways photosynthesis and plant growth are severely affected. The question is whether this strong mutant phenotype observed in double mutants can be simply explained by the additive effect of defects in both CET pathways. In this study, we used the weak mutant allele of pgr5-2 for the background of double mutants to avoid possible problems caused by the secondary effects due to the strong mutant phenotype. In two double mutants, crr2-2 pgr5-2 and ndhs-1 pgr5-2, the plant growth was unaffected and linear electron transport was only slightly affected. However, NPQ induction was more severely impaired in the double mutants than in the pgr5-2 single mutant. A similar trend was observed in the size of the proton motive force. Despite the slight reduction in photosystem II parameters, PSI parameters were severely affected in the pgr5-2 single mutant, the phenotype that was further enhanced by adding the NDH defects. Despite the lack of ?pH-dependent regulation at the cytochrome b6f complex (donor-side regulation of PSI), the plastoquinone pool was more reduced in the double mutants than in the pgr5-2 single mutants. This phenotype suggests that both PGR5/PGRL1- and NDH-dependent CET contribute to supply sufficient acceptors from PSI by balancing the ATP/NADPH production ratio. 相似文献
105.
Photosynthetic regulation under fluctuating light in field-grown Cerasus cerasoides: A comparison of young and mature leaves 总被引:1,自引:0,他引:1
Photosystem I (PSI) is a potential target of photoinhibition under fluctuating light. However, photosynthetic regulation under fluctuating light in field-grown plants is little known. Furthermore, it is unclear how young leaves protect PSI against fluctuating light under natural field conditions. In the present study, we examined chlorophyll fluorescence, P700 redox state and the electrochromic shift signal in the young and mature leaves of field-grown Cerasus cerasoides (Rosaceae). Within the first seconds after any increase in light intensity, young leaves showed higher proton gradient (ΔpH) across the thylakoid membranes than the mature leaves, preventing over-reduction of PSI in the young leaves. As a result, PSI was more tolerant to fluctuating light in the young leaves than in the mature leaves. Interestingly, after transition from low to high light, the activity of cyclic electron flow (CEF) in young leaves increased first to a high level and then decreased to a stable value, while this rapid stimulation of CEF was not observed in the mature leaves. Furthermore, the over-reduction of PSI significantly stimulated CEF in the young leaves but not in the mature leaves. Taken together, within the first seconds after any increase in illumination, the stimulation of CEF favors the rapid lumen acidification and optimizes the PSI redox state in the young leaves, protecting PSI against photoinhibition under fluctuating light in field-grown plants. 相似文献
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107.
Species extinctions alter ecosystem services, and the magnitude of this impact is likely to change across environmental gradients. In Australia, soil‐disturbing mammals that are now considered ecologically extinct are thought to be important ecosystem engineers. Previous studies have demonstrated microsite‐level impacts of reintroduced soil‐disturbing mammals on soil functions, but effects are yet to be tested across larger scales. Further, it is unclear how impacts vary across environmental gradients and if the restoration potential of reintroductions changes with climate. We examined the effects of soil‐disturbing mammal reintroductions across a large rainfall gradient in Australia to test the hypothesis that ecosystem engineering effects on soil function depend on climate. We compared soil labile carbon, available nitrogen and the activity of four enzymes associated with nutrient cycling in three microsite types with and without soil‐disturbing mammals in five sites along a large rainfall gradient (166–870 mm). Soil enzyme activity was greatest in the presence of soil‐disturbing mammals and increased with rainfall, but soil available carbon and nitrogen varied across the gradient and among microsites. Microsite effects were often stronger than any effects of soil‐disturbing mammals, with soil beneath vegetated patches (shrubs and trees) having greater enzyme activity, carbon and nitrogen than bare soils. However, soil‐disturbing mammals homogenised nutrient distributions across microsites. The impacts of soil‐disturbing mammals on soil function previously detected at micro‐scales was detected at a landscape‐scale. However, the overall effects of soil‐disturbing mammals on soil functions varied with productivity (rainfall). The context of soil‐disturbing mammal reintroductions is thus likely to be critical in determining their effectiveness in restoring soil function. 相似文献
108.
Charles W. Heckman Heino Kamieth Manfred Sthr 《International Review of Hydrobiology》1990,75(3):353-377
The data obtained during an extensive multidisciplinary investigation of a polluted harbor basin in Hamburg were analyzed in various ways in order to determine which statistical methods are easiest to interpret and permit the most accurate evaluations. The methods analyzed include the species deficit, index of species similarity, and saprobity index, as well as the more complex cluster and gradient analyses, which revealed a variety of relationships among the biotic communities at the various sampling sites and some ecological tolerance limits of various species. The shortcomings of numerical indices for characterizing water bodies are discussed. It is suggested that biostatistical methods are much more valuable for the empirical evaluation of data than for artificially classifying ecosystems. 相似文献
109.
110.
Neuregulins: functions,forms, and signaling strategies 总被引:35,自引:0,他引:35
Falls DL 《Experimental cell research》2003,284(1):14-30
The neuregulins (NRGs) are cell-cell signaling proteins that are ligands for receptor tyrosine kinases of the ErbB family. The neuregulin family of genes has four members: NRG1, NRG2, NRG3, and NRG4. Relatively little is known about the biological functions of the NRG2, 3, and 4 proteins, and they are considered in this review only briefly. The NRG1 proteins play essential roles in the nervous system, heart, and breast. There is also evidence for involvement of NRG signaling in the development and function of several other organ systems, and in human disease, including the pathogenesis of schizophrenia and breast cancer. There are many NRG1 isoforms, raising the question "Why so many neuregulins?" Study of mice with targeted mutations ("knockout mice") has demonstrated that isoforms differing in their N-terminal region or in their epidermal growth factor (EGF)-like domain differ in their in vivo functions. These differences in function might arise because of differences in expression pattern or might reflect differences in intrinsic biological characteristics. While differences in expression pattern certainly contribute to the observed differences in in vivo functions, there are also marked differences in intrinsic characteristics that may tailor isoforms for specific signaling requirements, a theme that will be emphasized in this review. 相似文献