全文获取类型
收费全文 | 842篇 |
免费 | 41篇 |
国内免费 | 25篇 |
出版年
2024年 | 3篇 |
2023年 | 7篇 |
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 9篇 |
2019年 | 18篇 |
2018年 | 16篇 |
2017年 | 16篇 |
2016年 | 14篇 |
2015年 | 29篇 |
2014年 | 66篇 |
2013年 | 56篇 |
2012年 | 69篇 |
2011年 | 94篇 |
2010年 | 73篇 |
2009年 | 16篇 |
2008年 | 35篇 |
2007年 | 31篇 |
2006年 | 20篇 |
2005年 | 21篇 |
2004年 | 33篇 |
2003年 | 21篇 |
2002年 | 26篇 |
2001年 | 11篇 |
2000年 | 9篇 |
1999年 | 9篇 |
1998年 | 13篇 |
1997年 | 14篇 |
1996年 | 13篇 |
1995年 | 16篇 |
1994年 | 10篇 |
1993年 | 13篇 |
1992年 | 7篇 |
1991年 | 15篇 |
1990年 | 8篇 |
1989年 | 10篇 |
1988年 | 7篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 7篇 |
1982年 | 11篇 |
1981年 | 7篇 |
1980年 | 8篇 |
1979年 | 8篇 |
1978年 | 11篇 |
1977年 | 1篇 |
1976年 | 1篇 |
排序方式: 共有908条查询结果,搜索用时 14 毫秒
841.
Recently, new and improved methods have been developed to measure translocation of membrane-active peptides (antimicrobial, cytolytic, and amphipathic cell-penetrating peptides) across lipid bilayer membranes. The hypothesis that translocation of membrane-active peptides across a lipid bilayer is determined by the Gibbs energy of insertion of the peptide into the bilayer is re-examined in the light of new experimental tests. The original hypothesis and its motivation are first revisited, examining some of the specific predictions that it generated, followed by the results of the initial tests. Translocation is understood as requiring two previous steps: binding and insertion in the membrane. The problem of peptide binding to membranes, its prediction, measurement, and calculation are addressed. Particular attention is given to understanding the reason for the need for amphipathic structures in the function of membrane-active peptides. Insertion into the membrane is then examined. Hydrophobicity scales are compared, and their influence on calculations is discussed. The relation between translocation and graded or all-or-none peptide-induced flux from or into lipid vesicles is also considered. Finally, the most recent work on translocation is examined, both experimental and from molecular dynamics simulations. This article is part of a Special Issue entitled: Interfacially Active Peptides and Proteins. Guest Editors: William C. Wimley and Kalina Hristova. 相似文献
842.
SET, the translocation breakpoint-encoded protein in acute undifferentiated leukemia (AUL), is identified as a 39-kDa phosphoprotein found predominantly in the cell nuclei [1994, J. Biol. Chem. 269,2258-2262]. SET is fused to a putative oncoprotein, CAN, in AUL and is thought to regulate the transformation potential of SET-CAN by its nuclear localization and phosphorylation. We investigated in detail the in vivo phosphorylation of SET. Phosphorylation of SET occurred in all human cell lines examined in vivo, primarily on serine residues. Endoproteinase Glu-C digestion of phosphorylated SET yielded two phosphopeptides. By radiosequencing, we identified the in vivo phosphorylation sites of SET as Ser9 and Ser24. The surrounding sequences of Ser9 and Ser24 contained an apparent consensus site sequence for protein kinase C. 相似文献
843.
S. L. Grokhovsky I. A. Il’icheva D. Yu. Nechipurenko L. A. Panchenko R. V. Polozov Yu. D. Nechipurenko 《Biophysics》2008,53(3):250-251
Looking for new means of assessing local conformational and dynamic heterogeneities in DNA structure, we have estimated the rates of phosphodiester bond cleavage in DNA fragments of known sequence caused by ultrasonic treatment. Among the 16 dinucleotide steps possible, those with 5′-ward cytosine [5′-d(CpN)-3′] are distinguished by significantly higher cleavage rates: CG > CA = CT > CC. The possible causes of this intriguing phenomenon are considered. 相似文献
844.
Summary The effect of Ca on the absorption and translocation of Mn, Zn and Cd in excised barley roots was studied using a multi-compartment
transport box technique. A radioisotope (54Mn,65Zn or115mCd)-labelled test solution was supplied to the apexes of excised roots and the distribution pattern in the roots was examined
in the absence or presence of Ca. Results obtained were as follows.
Addition of Ca to the test solution reduced the absorption of Mn and inhibited drastically its translocation in excised roots.
With increasing concentrations of Ca in test solutions, its inhibitory effects on the absorption and translocation of Mn became
severe.
Similar results were observed for the absorption and translocation of Zn. Ca in the test solution decreased the absorption
and inhibited drastically the translocation of Zn; as in the case of Mn, higher concentrations of Ca had severe effects on
these functions.
It was also evident that the addition of Ca to the test solution reduced the absorption of Cd at all levels of Cd concentration
(1, 10, and 100 μM). Cd absorption decreased with increasing concentrations of Ca in the test solution. However, Ca accelerated the translocation
of Cd in excised roots supplied with test solutions containing up to 10μM Cd. At 100μM Cd, addition of Ca caused a negligibly small acceleration of Cd translocation.
The accelerating effect of Ca on Cd translocation, especially “xylem exudation”, decreased markedly with the addition of 2,4-dinitrophenol,
but not with the addition of chloramphenicol or p-chloromercuribenzene sulphonic acid. When barley plants were supplied with
only CaSO4 during the entire growing period, that is, plants were not supplied with nutrient solution on the last day of this period,
Ca had no accelerating effect on Cd translocation in excised roots. 相似文献
845.
846.
P. W. Jones 《Biometrical journal. Biometrische Zeitschrift》1978,20(6):619-622
BAYESian fixed sample size and sequential procedures are considered for the estimation of the unknown parameter v of a uniform distribution over (0, v), under several loss functions. 相似文献
847.
848.
Summary Experiments were performed to measure the pH-sensitive steps in nodulation and symbiotic fixation byPisum sativum and isolate RP-212-1 ofRhizobium leguminosarum. An aeroponic system with rigorous pH control was used to obtain numerous effective nodules. After exposure to various pH levels, the following responses were measured: (1) legume root growth and development, (2) survival and growth rate of a single effective bacterial isolate, (3) degree of nodulation, (4) rate of nitrogen fixation, (5) plant biomass, and (6) nitrogen content of plants. Both bacterial growth and root development were adequate at all pH levels from 4.4 to 6.6, but efficient nodulation and nitrogen fixation did not occur at pH 4.8 and below. The processes required for symbiosis were about 10 times as sensitive to acidity as either bacterial growth or root growth alone. Nodulation was the most acid-sensitive step. 相似文献
849.
A. MEATS 《Physiological Entomology》1987,12(2):165-170
ABSTRACT. Survival time at subzero temperatures is related to both long-term thermal history and the rate at which the insects are cooled. Insects cooled at one degree per hour survive for up to 3 times as long at a given test temperature than do insects cooled at 1C/min. Survival times are significantly shorter than times taken to freeze. Survival time at an extreme high temperature is related to long-term thermal history but the rate of heating makes no difference. 相似文献
850.
Ping Wang Yongling Liu Tao Chen Wenhua Xu Jinmao You Yongjun Liu Yulin Li 《Phytochemical analysis : PCA》2013,24(6):603-607