全文获取类型
收费全文 | 959篇 |
免费 | 1篇 |
国内免费 | 14篇 |
专业分类
974篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 11篇 |
2020年 | 4篇 |
2019年 | 11篇 |
2018年 | 8篇 |
2017年 | 9篇 |
2016年 | 3篇 |
2015年 | 19篇 |
2014年 | 84篇 |
2013年 | 80篇 |
2012年 | 71篇 |
2011年 | 90篇 |
2010年 | 90篇 |
2009年 | 35篇 |
2008年 | 27篇 |
2007年 | 35篇 |
2006年 | 38篇 |
2005年 | 29篇 |
2004年 | 28篇 |
2003年 | 24篇 |
2002年 | 25篇 |
2001年 | 10篇 |
2000年 | 10篇 |
1999年 | 14篇 |
1998年 | 13篇 |
1997年 | 11篇 |
1996年 | 7篇 |
1995年 | 11篇 |
1994年 | 16篇 |
1993年 | 8篇 |
1992年 | 12篇 |
1991年 | 15篇 |
1990年 | 10篇 |
1989年 | 13篇 |
1988年 | 6篇 |
1987年 | 11篇 |
1986年 | 9篇 |
1985年 | 9篇 |
1984年 | 14篇 |
1983年 | 11篇 |
1982年 | 21篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有974条查询结果,搜索用时 0 毫秒
971.
Sayuri Sakuragi Tatsuo Shioda Jun-ichi Sakuragi 《Microbes and infection / Institut Pasteur》2019,21(2):119-123
About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this system, we analyzed the genome packaging modes of primate lentiviruses such as HIV-1, 2, SIVmac and SIVagm. The data suggested that the modes of all viruses analyzed were very similar. In addition, we observed that the Gag-AUG sequences of them played important roles for maintaining efficient packaging, other than the initiation of translation. 相似文献
972.
973.
Phosphorylation of the yeast ribosomal stalk. Functional effects and enzymes involved in the process
Juan P.G. Ballestaa Miguel A. Rodriguez-Gabriela German Boua Elisa Brionesa Reina Zambranoa Miguel Remachaa 《FEMS microbiology reviews》1999,23(5):537-550
The ribosomal stalk is directly involved in the interaction of the elongation factors with the ribosome during protein synthesis. The stalk is formed by a complex of five proteins, four small acidic polypeptides and a larger protein which directly interacts with the rRNA at the GTPase center. In eukaryotes the acidic components correspond to the 12-kDa P1 and P2 proteins, and the RNA binding component is the P0 protein. All these proteins are found phosphorylated in eukaryotic organisms, and previous in vitro data suggested this modification was involved in the activity of this structure. Results from mutational studies have shown that phosphorylation takes place at a serine residue close to the carboxy end of the P proteins. Modification of this serine residue does not affect the formation of the stalk and the activity of the ribosome in standard conditions but induces an osmoregulation-related phenotype at 37 degrees C. The phosphorylatable serine is part of a consensus casein kinase II phosphorylation site. However, although CKII seems to be responsible for part of the stalk phosphorylation in vivo, it is probably not the only enzyme in the cell able to perform this modification. Five protein kinases, RAPI, RAPII and RAPIII, in addition to the previously reported CKII and PK60 kinases, are able to phosphorylate the stalk proteins. A comparison of the five enzymes shows differences among them that suggest some specificity regarding the phosphorylation of the four yeast acidic proteins. It has been found that some typical effectors of the PKC kinase stimulate the in vitro phosphorylation of the stalk proteins. All the data suggest that although phosphorylation is not involved in the interaction of the acidic P proteins with the ribosome, it can affect the ribosome activity and might participate in a possible ribosome regulatory mechanism. 相似文献
974.