全文获取类型
收费全文 | 3252篇 |
免费 | 227篇 |
国内免费 | 404篇 |
出版年
2023年 | 38篇 |
2022年 | 59篇 |
2021年 | 72篇 |
2020年 | 68篇 |
2019年 | 105篇 |
2018年 | 98篇 |
2017年 | 82篇 |
2016年 | 91篇 |
2015年 | 109篇 |
2014年 | 134篇 |
2013年 | 180篇 |
2012年 | 106篇 |
2011年 | 114篇 |
2010年 | 126篇 |
2009年 | 137篇 |
2008年 | 174篇 |
2007年 | 145篇 |
2006年 | 159篇 |
2005年 | 182篇 |
2004年 | 153篇 |
2003年 | 143篇 |
2002年 | 153篇 |
2001年 | 105篇 |
2000年 | 117篇 |
1999年 | 87篇 |
1998年 | 76篇 |
1997年 | 62篇 |
1996年 | 54篇 |
1995年 | 76篇 |
1994年 | 55篇 |
1993年 | 53篇 |
1992年 | 55篇 |
1991年 | 44篇 |
1990年 | 36篇 |
1989年 | 55篇 |
1988年 | 54篇 |
1987年 | 51篇 |
1986年 | 37篇 |
1985年 | 45篇 |
1984年 | 44篇 |
1983年 | 33篇 |
1982年 | 28篇 |
1981年 | 24篇 |
1980年 | 14篇 |
1979年 | 17篇 |
1978年 | 9篇 |
1977年 | 11篇 |
1976年 | 8篇 |
1975年 | 3篇 |
1973年 | 1篇 |
排序方式: 共有3883条查询结果,搜索用时 421 毫秒
831.
Violaine Bonnefoy Marie-Claire Pascal Jeanine Ratouchniak Marc Chippaux 《Molecular & general genetics : MGG》1986,204(1):180-184
Summary Nitrate reductase is demonstrated to exert an autogenous control on its own synthesis. This effect requires the participation of the molybdenum cofactor. Use of strains in which the control region of the nar operon is mutated reveals two loci in this region: one, affected in strain LCB94, is common to both autoregulation and induction by nitrate while the other, mutated in strain LCB188, is specific for the induction by nitrate. It is proposed that the autogenous control prevents the unnecessary accumulation of the nitrate reductase subunits in the cytoplasm. 相似文献
832.
833.
834.
Lukas K. Tamm 《生物化学与生物物理学报:生物膜》2003,1614(1):14-23
Substantial progress has been made in recent years to augment the current understanding of structures and interactions that promote viral membrane fusion. This progress is reviewed with a particular emphasis on recently determined structures of viral fusion domains and their interactions with lipid membranes. The results from the different structural and thermodynamic experimental approaches are synthesized into a new proposed mechanism, termed the “spring-loaded boomerang” mechanism of membrane fusion, which is presented here as a hypothesis. 相似文献
835.
836.
Hisakazu Yamane Noboru Murofushi Nobutaka Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(1):207-210
A novel metabolite from Gibberella fujikuroi was isolated and its structure was elucidated as 4β,7β-dihydroxy-18-norkaurenolide (I). 相似文献
837.
838.
《Molecular membrane biology》2013,30(1):9-25
AbstractThe icosahedral Polio virus capsid consists of 60 copies of each of the coat proteins VP1, VP2, VP3 and myristolyated VP4 (myrVP4). Catalyzed by the host cell receptor the Polio virus enters the host cell via externalization of myrVP4 and the N terminal part of VP1. There are several assumptions about the individual role of both of the proteins in the mechanism of membrane attachment and genome injection. We use the first 32 N terminal amino acids of VP1 and applied molecular dynamics simulations to assess its mechanism of function when attached and inserted into hydrated lipid membranes (POPC). Helical models are placed in various positions in regard to the lipid membrane to start with. As a comparison, the first 33 amino acids of the fusion peptide of gp41 of HIV-1 are simulated under identical conditions. Computational data support the idea that VP1 is not penetrating into the membrane to form a pore; it rather lays on the membrane surface and only perturbs the membrane. Furthermore, this idea is strengthened by channel recordings of both peptides showing irregular openings. 相似文献
839.
Tek-Hyung Lee Timothy S. Carpenter Patrik D'haeseleer David F. Savage Mimi C. Yung 《Biotechnology and bioengineering》2020,117(3):603-613
Antimicrobial peptides (AMPs) are regarded as attractive alternatives to conventional antibiotics, but their production in microbes remains challenging due to their inherent bactericidal nature. To address these limitations, we have developed a novel AMP fusion protein system based on an encapsulin nanocompartment protein and have demonstrated its utility in enhancing expression of HBCM2, an AMP with activity against Gram-negative bacteria. Here, HBCM2 was fused to the N-terminus of several Encapsulin monomer (Enc) variants engineered with multiple TEV protease recognition site insertions to facilitate proteolytic release of the fused HBCM2. Fusion of HBCM2 to the Enc variants, but not other common carrier proteins, enabled robust overexpression in Escherichia coli C43(DE3) cells. Interestingly, variants with a TEV site insertion following residue K71 in Enc exhibited the highest overexpression and HBCM2 release efficiencies compared to other variants but were deficient in cage formation. HBCM2 was purified from the highest expressing variant following TEV protease digestion and was found to be highly active in inhibiting E. coli growth (MIC = 5 μg/ml). Our study demonstrates the potential use of the Enc system to enhance expression of AMPs for biomanufacturing and therapeutic applications. 相似文献
840.