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81.
A. Santarelli H. Brinkhuis F. J. Hilgen L. J. Lourens G. J. M. Versteegh H. Visscher 《Marine Micropaleontology》1998,33(3-4)
A high-resolution palynological study of the cyclically bedded Faneromeni section (upper Tortonian-lower Messinian) on Crete (Greece) is presented. This study aims to recognize orbitally-driven variations in the palynological record and to validate the age model based on the astronomical calibration of the sedimentary cycles. Four palynology-based environmental proxies were utilised using interpretations of fossil dinoflagellate associations based on modern ecological characteristics. Cross-spectral analysis between the proxy records and astronomical target curve, the 65°N summer insolation, yielded in most cases significant spectral power and coherence in the precession and/or obliquity frequency bands. Precession-controlled variations in the proxy records are related to lithology and indicate that maxima in continental input and minima in sea surface salinity coincide with sapropel formation. The influence of obliquity is most clearly reflected in the index of continental versus marine palynomorphs (S-D). The absence of a distinct time lag relative to obliquity indicates that the 41-kyr component in continental input is controlled by oscillations in regional Mediterranean climate rather than by glacial cyclicity. Phase relations in the different astronomical frequency bands of the spectrum, as compared with the Mediterranean Pliocene, essentially confirm the validity of the Miocene astronomical time scale. Finally, a major non-cyclic change in the palynological assemblage at 6.68 Ma indicates enhanced salinity and decreased river discharge. This shift coincides with a significant drop in sedimentation rate informally termed the “Early Messinian Sediment starvation Event”. 相似文献
82.
RONALD N. TOMAS 《The Journal of eukaryotic microbiology》1974,21(2):316-321
Mitosis and cytokinesis are described and illustrated for the first time in the mesokaryotic, catenate dinoflagellate Gonyaulax catenella. A structure similar to the central body of G. tamarensis and G. monilata is shown by light and electron microscopy to be situated intranuclearly near the arms of the U-shaped interphase nucleus, and is suggested to function in the segregation of daughter chromosomes. This structure has the fine structure of a nucleolus, and it is suggested that the term central body be replaced by persistent nucleolus (= endosome). The time required for the completion of mitosis is 4–6 hr, while cytokinesis requires at least 2 hr. Cytokinesis begins during the mitotic cycle, and the plane of fission is perpendicular to the mitotic plane of division. Parental fission moieties are retained and shared by the daughter cells while either a new antero-sinistral moiety or a posterodextral moiety is synthesized by the dividing cell. 相似文献
83.
Gymnoxanthella radiolariae gen. et sp. nov. (Dinophyceae), a dinoflagellate symbiont from solitary polycystine radiolarians 下载免费PDF全文
The symbiotic dinoflagellate Gymnoxanthella radiolariae T. Yuasa et T. Horiguchi gen. et sp. nov. isolated from polycystine radiolarians is described herein based on light, scanning and transmission electron microscopy as well as molecular phylogenetic analyses of SSU and LSU rDNA sequences. Motile cells of G. radiolariae were obtained in culture, and appeared to be unarmored. The cells were 9.1–11.4 μm long and 5.7–9.4 μm wide, and oval to elongate oval in the ventral view. They possessed an counterclockwise horseshoe‐shaped apical groove, a nuclear envelope with vesicular chambers, cingulum displacement with one cingulum width, and the nuclear fibrous connective; all of these are characteristics of Gymnodinium sensu stricto (Gymnodinium s.s.). Molecular phylogenetic analyses also indicated that G. radiolariae belongs to the clade of Gymnodinium s.s. However, in our molecular phylogenetic trees, G. radiolariae was distantly related to Gymnodinium fuscum, the type species of Gymnodinium. Based on the consistent morphological, genetic, and ecological divergence of our species with the other genera and species of Gymnodinium s.s., we considered it justified to erect a new, separate genus and species G. radiolariae gen. et sp. nov. As for the peridinioid symbiont of radiolarians, Brandtodinium has been erected as a new genus instead of Zooxanthella, but the name Zooxanthella is still valid. Brandtodinium is a junior synonym of Zooxanthella. Our results suggest that at least two dinoflagellate symbiont species, peridinioid Zooxanthella nutricula and gymnodinioid G. radiolariae, exist in radiolarians, and that they may have been mixed and reported as “Z. nutricula” since the 19th century. 相似文献
84.
The persistence of the larvicidal activity of Bacillus thuringiensis var. israelensis (Bti) was tested over a five-month period in a low-temperature aquatic environment. Diffusion chambers filled with a suspension of Bti (100 mg l - 1) and different experimental substrates (pond water, periphyton, sediments and vegetation), but without mosquito larvae, were placed near the bottom of a large pond and removed at various intervals to measure residual toxic activity to mosquito larvae, spore concentration and proteolytic activity. Within the pond water substrate, 50% of the initial toxicity was still present after one month in cold water, while with the periphyton substrate, 30% remained in the liquid fraction after the same period of exposure. Within the vegetation substrate (blue-joint grass, Calamagrostis canadensis), an average of 30% of the initial toxicity was still present in the liquid fraction between day 84 to day 154. Solid fractions of vegetation became toxic very early and remained toxic for five months. At the end (day 154), there was still 54% of the original toxic activity put in the chambers associated with the vegetation samples. In the absence of mosquito larvae, spore recycling was observed in the chambers especially with sediments and vegetation. But spore recycling did not appear to play a major role in the observed persistence, but rather rapid absorption onto vegetation substrates was responsible for the persistence of Bti in a cold climate. 相似文献
85.
86.
The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO2 下载免费PDF全文
This work originates from three facts: (i) changes in CO2 availability influence metabolic processes in algal cells; (ii) Spatial and temporal variations of nitrogen availability cause repercussions on phytoplankton physiology; (iii) Growth and cell composition are dependent on the stoichiometry of nutritional resources. In this study, we assess whether the impact of rising pCO2 is influenced by N availability, through the impact that it would have on the C/N stoichiometry, in conditions of N sufficiency. Our experiments used the dinoflagellate Protoceratium reticulatum, which we cultured under three CO2 regimes (400, 1,000, and 5,000 ppmv, pH of 8.1) and either variable (the NO3? concentration was always 2.5 mmol · L?1) or constant (NO3? concentration varied to maintain the same Ci/NO3? ratio at all pCO2) Ci/NO3? ratio. Regardless of N availability, cells had higher specific growth rates, but lower cell dry weight and C and N quotas, at elevated CO2. The carbohydrate pool size and the C/N was unaltered in all treatments. The lipid content only decreased at high pCO2 at constant Ci/NO3? ratio. In the variable Ci/NO3? conditions, the relative abundance of Rubisco (and other proteins) also changed; this did not occur at constant Ci/NO3?. Thus, the biomass quality of P. reticulatum for grazers was affected by the Ci/NO3? ratio in the environment and not only by the pCO2, both with respect to the size of the main organic pools and the composition of the expressed proteome. 相似文献
87.
Cultures of four strains of the dinoflagellate Gymnodinium aureolum (Hulburt) G. Hansen were established from the Elizabeth River, a tidal tributary of the Chesapeake Bay, USA. Light microscopy, scanning electron microscopy, nuclear-encoded large sub-unit rDNA sequencing, and culturing observations were conducted to further characterize this species. Observations of morphology included: a multiple structured apical groove; a peduncle located between the emerging points of the two flagella; pentagonal and hexagonal vesicles on the amphiesma; production and germination of resting cysts; variation in the location of the nucleus within the center of the cell; a longitudinal ventral concavity; and considerable variation in cell width/length and overall cell size. A fish bioassay using juvenile sheepshead minnows detected no ichthyotoxicity from any of the strains over a 48-h period. Molecular analysis confirmed the dinoflagellate was conspecific with G. aureolum strains from around the world, and formed a cluster along with several other Gymnodinium species. Morphological evidence suggests that further research is necessary to examine the relationship between G. aureolum and a possibly closely related species Gymnodinium maguelonnense. 相似文献
88.
Nagasaki K 《Journal of microbiology (Seoul, Korea)》2008,46(3):235-243
Since the first discovery of the very high virus abundance in marine environments, a number of researchers were fascinated with the world of "marine viruses", which had previously been mostly overlooked in studies on marine ecosystems. In the present paper, the possible role of viruses infecting marine eukaryotic microalgae is enlightened, especially summarizing the most up-to-the-minute information of marine viruses infecting bloom-forming dinoflagellates and diatoms. To author's knowledge, approximately 40 viruses infecting marine eukaryotic algae have been isolated and characterized to different extents. Among them, a double-stranded DNA (dsDNA) virus "HcV" and a single-stranded RNA (ssRNA) virus "HcRNAV" are the only dinoflagellate-infecting (lytic) viruses that were made into culture; their hosts are a bivalve-killing dinoflagellate Heterocapsa circularisquama. In this article, ecological relationship between H. circularisquama and its viruses is focused. On the other hand, several diatom-infecting viruses were recently isolated and partially characterized; among them, one is infectious to a pen-shaped bloom-forming diatom species Rhizosolenia setigera; some viruses are infectious to genus Chaetoceros which is one of the most abundant and diverse diatom group. Although the ecological relationships between diatoms and their viruses have not been sufficiently elucidated, viral infection is considered to be one of the significant factors affecting dynamics of diatoms in nature. Besides, both the dinoflagellate-infecting viruses and diatom-infecting viruses are so unique from the viewpoint of virus taxonomy; they are remarkably different from any other viruses ever reported. Studies on these viruses lead to an idea that ocean may be a treasury of novel viruses equipped with fascinating functions and ecological roles. 相似文献
89.
Cryptophyte vestiges showing selective digestion of nuclei were found in the gonyaulacalean dinoflagellates Amylax buxus (Balech) Dodge and Amylax triacantha (Jörgensen) Sournia. They emitted bright yellow‐orange fluorescence (590‐nm emission) under epifluorescent microscopy and possessed U‐shaped plastids, suggesting the vestiges were active in photosynthesis. Under transmission electron microscopy, the plastid was characterized by a loose arrangement of two to three thylakoid stacks and included a stalked pyrenoid, as in the cryptophyte genus Teleaulax. Indeed, molecular data based on the plastid small‐subunit rRNA gene demonstrated that the vestiges in Amylax originated from Teleaulax amphioxeia. The stolen plastid (kleptoplastids) in Dinophysis is also derived from this cryptophyte species. However, in sharp contrast to Dinophysis, the plastid of the vestige in Amylax was surrounded by a double layer of plastid endoplasmic reticulum, and within the periplastidal area, a nucleomorph was retained. The vestiges also possessed mitochondria with characteristic plate‐like cristae, but lost the cell‐surface structure. The phagocytotic membrane of the dinoflagellates seemed to surround the cryptophytes right after the incorporation, but the membrane itself would probably be digested eventually. Remarkably, only one cryptophyte cell among 14 vestiges in a cell of A. buxus had a nucleus. This is the first recording of possible kleptoplastidy in gonyaulacalean dinoflagellates, and documents the strategy of a dinoflagellate involving the selective elimination of the cryptophyte nucleus. 相似文献
90.