Foliar macronutrients (N,P, K,Ca and Mg) in lime (Tilia spp.)

Summary The effect of sampling technique upon the measurement of variation in nutrient (N, P, K, Ca and Mg) concentration was examined. Sampling and analytical precision over two years were indicated by coefficients of variation which ranged from 5.7 to 14.0 per cent for sampling error and 4.3 to 16.1 per cent for analytical error. Concentration of N, P and K in the leaves was found to be independent of sampling height. Ca and Mg concentrations, however, decreased with increased height on the crown. The differences in concentration between the bottom and top of the crown were 15 and 19 per cent for Ca and Mg respectively. The nutrient concentrations of samples taken from basal coppice shoots were significantly different from those in the true crown. Nutrient concentration was independent of aspect. Leaf area varied throghout the season, but the largest leaves were always at the base of the crown and the smallest at the top.     

长白山阔叶红松林紫椴种群结构与动态特征

紫椴(Tilia amurensis)为国家二级重点保护野生植物。以长白山北坡阔叶红松林内的紫椴种群为研究对象,通过建立种群静态生命表、绘制存活曲线、计算数量动态指数并引入生存函数和时间序列预测模型定量描绘其结构与动态特征。结果表明:1)种群年龄结构呈不规则金字塔形,幼龄阶段(Ⅰ-Ⅳ龄级)个体数之和占个体总数的67.26%。2)种群存活曲线接近Deevey-Ⅲ型,表明植株在幼龄阶段死亡率高。3)总体数量动态变化指数V_pi>V''_pi>0,表明紫椴种群为增长型,但V''_pi趋近于0,结合死亡率(q_x)和消失率(K_x)曲线呈现出连续性先增后降的复杂动态变化趋势,可知该种群不稳定且在干扰情况下增长趋势不明显。4)生存函数分析显示,种群在Ⅲ-Ⅳ龄级间达到平衡状态,尔后进入衰退期且从第Ⅷ龄级开始进入生理衰退。5)时间序列预测分析表明,在未来2、3、4、5、6、7、8、9、10龄级时间后,紫椴幼龄个体数逐渐减小,而中、成和老龄个体数量将逐渐增加,说明未来紫椴种群的增长主要依靠后者。生物学特性和生境是限制紫椴种群增长的主要原因。建议加强幼苗抚育工作,提高幼苗和小树的存活率,保护和改善生存环境,从而促进紫椴种群的自然更新和恢复。     

华东椴花序苞片和叶片发育及结构特征比较研究

花序苞片是椴树属最典型的特征,但它与其他器官的演化关系尚不明确。本文对华东椴（Tilia japonica Simonk.）花序苞片和叶片在生长发育上的关联性进行分析,并对其表面微形态特征和内部解剖结构进行详细比较。结果显示,华东椴苞片和叶片在生长发育过程中表现出非常相似的规律性,二者在苞片幼期阶段均生长迅速、中期阶段生长变缓、成熟阶段再有小幅生长。对苞片与叶片形态性状的相关性分析显示,苞片长、苞片宽、叶长、叶宽的相关性均极显著。在表面微形态特征上,苞片和叶片总体上非常相似;在解剖特征上,苞片没有栅栏组织和海绵组织的分化,叶片有明显的分化。结合椴树属苞片的化石记录,我们认为椴树属苞片最早由叶片特化而来,是一种叶状结构,二者在表皮微形态特征及解剖结构上的差异,主要是在演化过程中为了适应各自不同功能而逐渐产生的。     

Fruit-bearing branchlets are carbon autonomous in mature broad-leaved temperate forest trees

In order to evaluate the degree of carbon autonomy for fruit development, the carbon source-sink relationship in fruit-bearing branchlets of mature deciduous forest trees was manipulated in situ. The tests included half and complete defoliation, girdling or the combination of both treatments, which were applied on fruiting branchlets by using a canopy crane. Concentrations of non-structural carbohydrates (NSC) were analysed in different branchlet tissues and fruits, to identify situations of carbon imbalances induced by the treatments. NSC concentrations of branchlets were generally lower under treatments resulting in decreased fruit growth. All three investigated species (Carpinus betulus, Fagus sylvatica and Tilia platyphyllos) exhibited complete carbon autonomy of fruiting at the level of whole, undisturbed branchlets, since neither a decrease of total infructescence biomass, nor of individual fruit mass occurred on girdled, un-defoliated branchlets. On girdled, 100% defoliated branchlets, fruit biomass relative to controls decreased by approximately 50% in Carpinus and Tilia, but by almost 80% in Fagus, which can be explained by different proportions of photosynthetically active infructescence tissues among the species. In contrast to the other two species, Tilia branchlets did not import carbon to compensate for assimilate loss after defoliation.     

Protein limitation of a tree leaf feeding Lepidopteran

The hypothesis that arbivorous insects are protein limited was tested by feeding larvae of Datana ministra mature leaves of Tilia americana that were coated with a mixture of protein. Larvae fed the protein supplement had a lower ingestion rate and net growth efficienty but higher apparent digestion efficiency and apparent assimilation rate than control larvae. Protein supplement had no effect on the rate of growth of 5th instar larvae although larvae fed added protein pupated at a lower weight than control larvae. The added protein placed a metabolic load on the larvae, perhaps by excessive nitrogen excretion demands and osmotic imbalance. The results do not support the hypothesis that arbivorous insects are protein limited.

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Résumé L'hypothèse suivant laquelle la croissance des larves d'insectes est limitée par l'insuffisance des protéines disponibles dans les feuilles d'arbres a été examinée par l'alimentation des chenilles de Datana ministra Drury sur feuilles développées de Tilia americana L. enduites d'une mixture de protéines d'oeuf et de lait. Des chenilles de second stade, prélevées dans des colonies naturelles, ont été séparées en un lot témoin élevé en masse sur feuilles coupées et un lot expérimental élevé sur feuiles coupées mais enduites de protéines. Le bilan en matière sèche, le taux de croissance, la relation entre ce taux de croissance et la quantité de matière ingérée, le poids des prénymphes ont été déterminés sur 10 chenilles de dernier stade dans chaque lot. Les chenilles ayant consommé un supplément de protéine ont présenté un plus faible taux d'ingestion et un plus faible rendement de croissance, mais une meilleure efficacité apparente d'ingestion et un meilleur taux apparent d'assimilation que les témoins.Le complément protéique n'a pas eu d'effet sur le taux de croissance des chenilles de 5ème stade bien que l'alimentation avec addition de protéines ait provoqué la nymphose à un poids larvaire plus faible. L'addition de protéines avait provoqué une chargé métabolique pour les chenilles, peut-être en provoquant une demande d'excrétion d'azote excessive ou en créant un déséquilibre osmotique, ce qui aurait provoqué une réduction du taux de croissance et du poids des chenilles malgré un taux d'assimilation supérieur. Ces résultats ne confirment pas l'hypothèse que les insectes des arbres sont carencés en protéines.

     

紫椴、水曲柳人工幼林霜冻发生与控制研究

采用垂直梯度观测与样地调查相结合的方法,对紫椴和水曲柳人工幼林最低温度变化、物候及霜冻程度进行了系统研究和分析,同时进行了防止紫椴晚霜冻田间试验.结果表明,紫椴的物候较水曲柳晚;受逆温现象的影响,不同坡向和坡位的紫椴和水曲柳物候变化表现出特殊的规律性,西坡芽苞开放时间较东坡早,芽苞开放随坡位的上升而提前,并以坡上最早;晚霜的发生是早春季节降温过程与该阶段林木物候变化动态吻合的结果;通过立地选择可以有效地控制霜冻,该地区坡下特别是东坡的坡下不适合营造紫椴和水曲柳人工林;化学物质和生物遮荫可以推迟紫椴萌动,进而防止霜冻;混交林对防止霜冻具有明显效果,无论是霜冻指数还是各受害等级的株数比例均低于纯林.     

MALDI-TOF mass spectrometry and PSD fragmentation as means for the analysis of condensed tannins in plant leaves and needles

MALDI-TOF mass spectrometry and 13C NMR spectroscopy were applied to unveil typical characteristics of condensed tannins of leaves and needles from willow (Salix alba), spruce (Picea abies) and beech (Fagus sylvatica) of three tree species that are ubiquitous in German forests and landscapes. For further evaluation, lime (Tilia cordata) was included. The 13C NMR spectroscopy confirmed the purity of the condensed tannin fractions and the efficiency of the procedure used for their extraction. While signals representative for procyanidin units are observable in all liquid-state 13C NMR spectra, resonance lines of prodelphinidin were only detected in those obtained from the condensed tannins of spruce needles and beech leaves. Typical signals in the chemical shift region between 70 and 90 ppm demonstrated the presence of stereoisomers (catechin/epicatechin; gallocatechin/ epigallocatechin). The MALDI-TOF mass spectra of the condensed tannins show signals of polymers of up to undecamers. Supporting the observations from the NMR spectroscopy, the mass spectra of the willow and lime leaf condensed tannins were identified as polymers with mainly procyanidin units, while the polymers of the spruce needle and beech leaves exhibit varying procyanidin/prodelphinidin ratios. Post source decay (PSD) fragmentation lead to a sequential loss of monomers and allowed a detailed characterization and sequencing of individual chains. In the case of the condensed tannins of lime this technique clearly excludes a pelargonidin terminal unit followed by a prodelphinidin unit, which would result in the same molecular masses as a polymer solely built up of procyanidin units.     

Drought acclimation of two deciduous tree species of different layers in a temperate forest canopy

Water-use strategies of Populus tremula and Tilia cordata, and the role of abscisic acid in these strategies, were analysed. P. tremula dominated in the overstorey and T. cordata in the lower layer of the tree canopy of the temperate deciduous forest canopy. Shoot water potential (), bulk-leaf abscisic acid concentration ([ABA]_leaf), abscisic acid concentration in xylem sap ([ABA]_xyl), and rate of stomatal closure following the supply of exogenous ABA (v) decreased acropetally through the whole tree canopy, and foliar water content per area (w), concentration of the leaf osmoticum (c), maximum leaf-specific hydraulic conductance of shoot (L), stomatal conductance (g_s), and the threshold dose per leaf area of the exogenous ABA (d_a) required to reduce stomatal conductance increased acropetally through the tree canopy (from the base of the foliage of T. cordata to the top of the foliage of P. tremula) in non-stressed trees. The threshold dose per leaf dry mass of the exogenous ABA (d_w) required to reduce stomatal conductance, was similar through the tree canopy. After a drought period (3 weeks), the , w, L, g_s, d_a and d_w had decreased, and c and v had increased in both species. Yet, the effect of the drought period was more pronounced on L, g_s, d_a, d_w and v in T. cordata, and on , w and c in P. tremula. It was concluded that the water use of the species of the lower canopy layer—T. cordata, is more conservative than that of the species of the overstorey, P. tremula. [ABA]_leaf had not been significantly changed in these trees, and [ABA]_xyl had increased during the drought period only in P. tremula. The relations between [ABA]_leaf, [ABA]_xyl and the stomatal conductance, the osmotic adjustment and the shoot hydraulic conductance are also discussed.     

基于不同预测变量的天然椴树可加性地上生物量模型构建

森林生物量是森林生态系统的最基本数量特征,生物量数据是研究许多林业问题和生态问题的基础,因此,准确测定生物量对于计算碳储量以及研究气候变化、森林健康、森林生产力、养分循环等十分重要.目前,测算森林生物量常用的方法为生物量模型估算法.本研究基于小兴安岭地区和张广才岭地区97株实测生物量数据,建立了3个天然椴树立木可加性生物量模型系统(基于胸径的一元可加性生物量模型系统、基于胸径和树高的二元可加性生物量模型系统、基于最优变量的最优可加性生物量模型系统),采用非线性似乎不相关回归法进行参数估计,用加权方法解决模型的异方差问题,并采用“刀切法”进行模型检验.结果表明: 3种可加性生物量模型系统均能较好地对椴树各部分生物量进行拟合和预测(调整后确定系数R_a²>0.84,平均预测误差百分比MPE<8.5%,平均绝对误差MAE<16.3 kg,平均百分标准误差MPSE<28.5%),其中,树干和地上生物量的拟合效果优于树叶、树枝和树冠;在引入树高和树冠因子后,提高了模型的拟合效果和预测能力(R_a²提高0.01～0.04,MAE降低0.01～4.55 kg),缩小了预测值置信区间的范围,树干、树叶和地上生物量提高较多,树枝和树冠提高较少.总体来看,最优生物量模型系统效果最好,其次为二元生物量模型系统,再次是一元生物量模型系统,添加树高和树冠因子进行生物量模型的构建十分必要.     

Developmental plasticity of glandular trichomes into somatic embryogenesis in Tilia amurensis

BACKGROUND AND AIMS: In Tilia amurensis, two types of trichomes (hairy and glandular) develop from epidermal surfaces of cotyledons and hypocotyls of zygotic embryos soon after germination. Here, it is demonstrated that glandular trichome initials develop directly into somatic embryos when treated in vitro with 2,4-dichlorophenoxyacetic acid (2,4-D). METHODS: Zygotic embryos of Tilia amurensis were cultured on Murashige and Skoog medium with 3 % sucrose and various concentrations (0, 2.2, 4.4 and 8.8 microm) of 2,4-D. Morphological development of trichomes and somatic embryos was analysed by scanning electron microscope and light microscope after histological sectioning. KEY RESULTS: In zygotic embryos cultured on medium with 4.4 microM 2,4-D, formation of hairy trichomes was completely suppressed but formation of glandular trichome initials increased. That some filamentous trichome initials developed directly into somatic embryos was confirmed by histological and scanning electron microscope observation. When explants with different stages of trichome initials (two-, four- and eight-celled filamentous and fully mature trichomes) were temporally pre-treated with 4.4 microM 2,4-D for 24 h and transferred into hormone-free medium, two-celled and four-celled filamentous trichome initials were the effective stage of trichomes for somatic embryo induction. CONCLUSIONS: It is suggested that early developing filamentous trichome initials have developmental plasticity and that with 2,4-D treatment these trichome initials develop directly into somatic embryos.