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161.
Stephan Brandt Christina Walz Martina Schad Nada Pavlovic Julia Kehr 《Plant Molecular Biology Reporter》2003,21(4):417-427
Many physiological processes are limited to specific tissues or even specific cell types. Analysing entire plants or organs
results in averaged data of all cell types contained in the sample; thus, specific metabolic functions cannot be assigned
to individual cell types. A higher spatial resolution is required. By microdissecting plant organs, homogeneous material can
be obtained. If a suitable amount of material is collected, standard analytical methods can be applied to elucidate cell type-specific
processes. The collection of sufficient quantities of homogeneous material can be done by means of mechanical microdissection.
This technique is a low-cost alternative to laser-coupled microdissection techniques. Here we describe a protocol for chisel-assisted
mechanical microdissection of embedded plant material and demonstrate that the collected material is suitable to obtain nucleic
acids and proteins. 相似文献
162.
《Biotechnic & histochemistry》2013,88(5):289-292
Yeast mlcrocolonies grown on 5 × 10 nun rectangles of membrane (Millipore) filter are treated through the membrane pores with 0.5% sodium azide for 2-3 hr, 10% perchloric acid for 4 days, and Giemsa stain for 1 day. After dehydration in air, they are permanently mounted. Similar preparations can be made with cells from sporularion cultures. 相似文献
163.
Fourier Transform-Infrared [FT-IR] microscopy is a combination of instrumentation from which information can be derived about the structure and composition of materials; however, it presents unique problems for sample preparation. Traditional methods of preparing fiber cross sections employ embedding media such as methacrylates, epoxides and polyvinyl alcohols, all of which have groups in common with the cellulose molecule, and absorb in the same regions of the IR spectrum. Therefore, a new embedding method employing polystyrene has been developed for the preparation of cross and longitudinal sections of cellulosic fibers. Although polystyrene is a strong IR absorbing material, it can be completely removed from specimens prior to analysis. In addition, FT-IR spectra of cross sections have better resolution than conventional preparation methods employing ground samples prepared in a KBr disk. 相似文献