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871.
872.
Bernard D. Goldstein 《人类与生态风险评估》2002,8(6):1223-1228
Hazard identification is based upon the second “law” of toxicology, the specificity of toxic effects caused by a chemical agent. Specificity reflects the differential reactivity inherent in chemical structure and in the biological niches in which chemicals interact. Just as Paracelsus is identified with the first “law” of toxicology, the dose makes the poison, Paré, a century French surgeon, should be credited with an early formulation of the second “law” of toxicology, the specificity of chemical effects. I discuss a number of aspects of hazard identification, including issues related to oxygenated fuels, to routine safety assessment, to the interpretation of hematological neoplasms and to the Precautionary Principle. 相似文献
873.
《MABS-AUSTIN》2013,5(3):349-361
The selective cell surface expression of receptor tyrosine kinase-like orphan receptor 1 (ROR1) in chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) has made ROR1 a novel and promising target for therapeutic monoclonal antibodies (mAbs). Four mouse mAbs generated by hybridoma technology exhibited specific binding to human ROR1. Epitope mapping studies showed that two mAbs (2A2 and 2D11) recognized N-terminal epitopes in the extracellular region of ROR1 and the other two (1A1 and 1A7) recognized C-terminal epitopes. A ROR1- immunotoxin (BT-1) consisting of truncated Pseudomonas exotoxin A (PE38) and the VH and VL fragments of 2A2-IgG was made recombinantly. Both 2A2-IgG and BT-1 showed dose-dependent and selective binding to primary CLL and MCL cells and MCL cell lines. Kinetic analyses revealed 0.12-nM (2A2-IgG) to 65-nM (BT-1) avidity/affinity to hROR1, depicting bivalent and monovalent interactions, respectively. After binding to cell surface ROR1, 2A2-IgG and BT-1 were partially internalized by primary CLL cells and MCL cell lines, and BT-1 induced profound apoptosis of ROR1-expressing MCL cell lines in vitro (EC50 = 16 pM–16 nM), but did not affect ROR1-negative cell lines. Our data suggest that ROR1-immunotoxins such as BT-1 could serve as targeted therapeutic agents for ROR1-expressing B cell malignancies and other cancers. 相似文献
874.
Felipe de Azevedo Rosas Sergio Leonardo Favareto Garles Miller Matias Vieira Mariana Netto de Oliveira Felipe DAlmeida Costa Douglas Guedes de Castro 《Reports of Practical Oncology and Radiotherapy》2021,26(1):138
Primary MALT lymphoma arising at the dura is a rare circumstance with no categorical therapeutic plan in literature. There are few reports available with different treatment courses. Here, we report two cases with a long-term follow-up after the same pattern of management and review the literature. 相似文献
875.
876.
877.
Reformation in chimeric antigen receptor based cancer immunotherapy: Redirecting natural killer cell
Natural killer (NK) cells are an important subset of lymphocytes which play a critical role in host immunity against cancers. With MHC-independent recognition, short lifespan and potent cytotoxicity, NK cells make a promising candidate for chimeric antigen receptor (CAR)-engineered cancer immunotherapy. Due to innate biological properties of NK cells, CAR-NK may outperform CAR-T therapy in terms of less side effects and more universal access, which may become a great reformation in CAR-based cancer immunotherapy. The CARs used in peripheral blood (PB) NK cells as well as NK cell line like NK-92 are the most important outfits defining antigenic specificity. The constructs of CARs used in NK cells from different sources vary, which all undergo generational optimization. The anti-tumor effects of CAR-NK have been validated in numerous preclinical trials for cancers, including hematologic malignancies and many solid tumors, which provide evidence for potential clinical application of CAR-NK. Additionally, this review concludes the challenges faced in the application of CAR-NK. Although CAR-NK is considered as one of the most possible “off-the-shelf” products, the improvement for the efficiency of expansion and transduction as well as the solution for underlying safety issues is still needed. Possible coping strategies for challenges and upgrades in techniques are also highlighted for future development in CAR-NK cancer immunotherapy. 相似文献
878.
Vitaliy O. Kaminskyy Maxim D. Lootsik Rostyslav S. Stoika 《Central European Journal of Biology》2006,1(1):2-15
The purpose of this study was to examine the relationship between the DNA intercalating characteristics and the DNA damaging
capacity of four alkaloids extracted from Chelidonium majus L, as well as their toxicity towards murine NK/Ly lymphoma cells. Chelerythrine, sanguinarine and coptisine were found to be
intercalated into the DNA isolated from NK/Ly cells, meanwhile, chelidonine exhibited no affinity to DNA. Sanguinarine exhibited
the greatest toxicity toward NK/Ly cells, and the toxicity of the other three decreased in descending order: chelerythrine,
coptisine and chelidonine. Chelerythrine and sanguinarine caused DNA damage, illustrated by the formation of comets of the
third class. Coptisine was less toxic than chelerythrine and sanguinarine, and affected the formation the same class of comets
in higher concentration. The quantity of comets induced by chelidonine were negligible, a finding consistent with its inability
to intercalate into DNA structure. The ability of four main alkaloids of Chelidonium majus L., to intercalate into DNA isolated from murine NK/Ly lymphoma cells, correlated with their ability to induce breaks in cellular
DNA and with their toxic effect towards those cells. 相似文献
879.
Jingyu Deng Han Liang Qiuping Dong Yachao Hou Xingming Xie Jun Yu Daiming Fan Xishan Hao 《Open biology》2014,4(7)
The methylation of B-cell CLL/lymphoma 6 member B (BCL6B) DNA promoter was detected in several malignancies. Here, we quantitatively detect the methylated status of CpG sites of BCL6B DNA promoter of 459 patients with gastric cancer (GC) by using bisulfite gene sequencing. We show that patients with three or more methylated CpG sites in the BCL6B promoter were significantly associated with poor survival. Furthermore, by using the Akaike information criterion value calculation, we show that the methylated count of BCL6B promoter was identified to be the optimal prognostic predictor of GC patients. 相似文献
880.
The complexity of cancer and the vast amount of experimental data available have made computer-aided approaches necessary. Biomolecular modelling techniques are becoming increasingly easier to use, whereas hardware and software are becoming better and cheaper. Cross-talk between theoretical and experimental scientists dealing with cancer-research from a molecular approach, however, is still uncommon. This is in contrast to other fields, such as amyloid-related diseases, where molecular modelling studies are widely acknowledged. The aim of this review paper is therefore to expose some of the more common approaches in molecular modelling to cancer scientists in simple terms, illustrating success stories while also revealing the limitations of computational studies at the molecular level. 相似文献