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101.
102.
混合盐碱胁迫对青山杨渗透调节物质及活性氧代谢的影响 总被引:6,自引:0,他引:6
为研究青山杨(Populus pseudo-cathayana × P. deltoides)对盐碱的适应能力,对青山杨2年生扦插苗进行不同盐度和碱度的28组胁迫处理.结果表明:随盐浓度增加,青山杨叶片的电解质外渗率、丙二醛和脯氨酸含量呈上升趋势,可溶性糖、SOD和POD活性先升后降.pH值升高使电解质外渗率、丙二醛和POD活性呈上升趋势,脯氨酸和可溶性糖含量先升后降,SOD活性上升趋势不明显.盐浓度低于100 mmol·L-1时,随pH值升高,各项生理指标的变化不明显,SOD具有较高的活性;盐浓度在200 mmol·L-1、pH 8.99以上时,其电解质外渗率在50%以上,POD活性和丙二醛含量大幅度增加,脯氨酸和可溶性糖含量下降,SOD活性较低.推断盐浓度>200 mmol·L-1、pH>8.99的盐碱条件不适宜青山杨的生长. 相似文献
103.
The effects of cultural conditions on growth and secondary metabolism in Streptomyces thermoviolaceus 总被引:2,自引:0,他引:2
Abstract Granaticin, an isochromate quinone antibiotic is synthesized as a secondary metabolite by Streptomyces thermoviolaceus . Antibiotic productivity was investigated under a variety of cultural conditions, including complex and defined media, mesophilic and thermophilic temperatures and a variety of sole carbon sources. In a defined medium growth was supported, to varying extents, by different carbon sources and in most cases granaticin production was observed. Highest biomass and granaticin yields were obtained when cultures were grown in the presence of xylan, fructose, glutamate or proline as carbon source. Changes in pH during growth affected both the timing and extent of granaticin production. 相似文献
104.
Disturbed nitrogen metabolism associated with the hyperhydric status of fully habituated callus of sugarbeet 总被引:2,自引:0,他引:2
Frédérik Le Dily Jean-Pierre Billard Thomas Gaspar Claude Huault 《Physiologia plantarum》1993,88(1):129-134
The content of polyamines and proline was much lower in a normal (N) callus of Beta vulgaris L. than in a fully habituated hyperhydric (H) callus. The H callus also contained more glutamate and had a higher glutamate dehydrogenase activity. The excess of glutamate, in this chlorophyll-deficient callus, was linked to accumulation of proline and polyamines. Experiments with α-difluoromethylornithine (DFMO) and α-difluoromethylarginine (DFMA) showed that both ornithine decarboxylase and arginine decarboxylase participated in the synthesis of polyamines (especially spermidine and putrescine) and removal of ammonia. It is hypothesized that the H callus was subjected to ammonia stress from the start of the culture. Experiments with gabaculine, an inhibitor of ornithine aminotransferase, showed that this enzyme linked proline degradation to polyamine synthesis through the production of ornithine. This disturbed nitrogen metabolism appeared to be characteristic of the fully habituated callus and might explain the low growth of this hyperhydric tissue. 相似文献
105.
SYNOPSIS. Plasmodium lophurae serine hydroxymethyltransferase (EC 2.1.2.1) was partially purified and characterized by (NH4)2SO4 fractionation and chromatography on Sephadex G-100. The enzyme, precipitated by 3.0–3.3 m (NH4)2SO4, had a molecular weight of 68,300 as estimated by exclusion chromatography on G-100. The pH optimum of the enzyme was 6.8–7.6 in sodium phosphate-citrate buffer. Citrate stabilized the enzyme during storage in phosphate buffer at 4 C. The Km was 4.3 × 10?3m for l -serine and 2.5 × 10?4m for tetrahydrofolate. 相似文献
106.
Eugenol specialty chemical production in transgenic poplar (Populus tremula × P. alba) field trials 下载免费PDF全文
Joaquim V. Marques P. Pawan Chakravarthy Syed G. A. Moinuddin Randi Luchterhand Barri Herman Laurence B. Davin Norman G. Lewis 《Plant biotechnology journal》2017,15(8):970-981
A foundational study assessed effects of biochemical pathway introduction into poplar to produce eugenol, chavicol, p‐anol, isoeugenol and their sequestered storage products, from potentially available substrates, coniferyl and p‐coumaryl alcohols. At the onset, it was unknown whether significant carbon flux to monolignols vs. other phenylpropanoid (acetate) pathway metabolites would be kinetically favoured. Various transgenic poplar lines generated eugenol and chavicol glucosides in ca. 0.45% (~0.35 and ~0.1%, respectively) of dry weight foliage tissue in field trials, as well as their corresponding aglycones in trace amounts. There were only traces of any of these metabolites in branch tissues, even after ~4‐year field trials. Levels of bioproduct accumulation in foliage plateaued, even at the lowest introduced gene expression levels, suggesting limited monolignol substrate availability. Nevertheless, this level still allows foliage collection for platform chemical production, with the remaining (stem) biomass available for wood, pulp/paper and bioenergy product purposes. Several transformed lines displayed unexpected precocious flowering after 4‐year field trial growth. This necessitated terminating (felling) these particular plants, as USDA APHIS prohibits the possibility of their interacting (cross‐pollination, etc.) with wild‐type (native plant) lines. In future, additional biotechnological approaches can be employed (e.g. gene editing) to produce sterile plant lines, to avoid such complications. While increased gene expression did not increase target bioproduct accumulation, the exciting possibility now exists of significantly increasing their amounts (e.g. 10‐ to 40‐fold plus) in foliage and stems via systematic deployment of numerous ‘omics’, systems biology, synthetic biology and metabolic flux modelling approaches. 相似文献
107.
Donald B. Galbraith George L. Wolff Nancy L. Brewer 《Genesis (New York, N.Y. : 2000)》1979,1(2):167-179
This study was conducted to assess microenvironmental variability within integumental tissue of genetically identical mice with respect to a specific cellular response: cyclic synthesis of yellow and black pigment by hair bulb melanocytes. Crosses were performed within and between inbred strains of mice that were isogenic with the exception of a single gene substitution at the agouti locus. Agouti locus genes included the Avy, Aw, A, atd, at, ax, am, and a alleles. The pigment patterns of dorsal, flank, and ventral hairs of the first and third hair generations and of hairs growing in special integumentary areas such as the pinna, tail, and hind foot were studied. It was found that the amount of yellow pigment synthesized by hair bulb melanocytes within genetically identical mice is both agedependent and conditioned by the integumentary environment. Furthermore, the special integumentary regions produce hairs with a variety of pigment patterns in which the distribution and relative amounts of black and yellow pigments do not necessarily conform to dominance relationships expected among agouti locus alleles as judged by their effects on the pigmentation of the dorsal pelage. We conclude that within genetically uniform integumental tissues, microenvironmental differences occur and are reflected as alterations in the metabolic pattern of differentiated cells. 相似文献
108.
Mahtab Tavasoli Sarah Lahire Taryn Reid Maren Brodovsky Christopher R. McMaster 《The Journal of biological chemistry》2020,295(51):17877
The two branches of the Kennedy pathways (CDP-choline and CDP-ethanolamine) are the predominant pathways responsible for the synthesis of the most abundant phospholipids, phosphatidylcholine and phosphatidylethanolamine, respectively, in mammalian membranes. Recently, hereditary diseases associated with single gene mutations in the Kennedy pathways have been identified. Interestingly, genetic diseases within the same pathway vary greatly, ranging from muscular dystrophy to spastic paraplegia to a childhood blinding disorder to bone deformations. Indeed, different point mutations in the same gene (PCYT1; CCTα) result in at least three distinct diseases. In this review, we will summarize and review the genetic diseases associated with mutations in genes of the Kennedy pathway for phospholipid synthesis. These single-gene disorders provide insight, indeed direct genotype-phenotype relationships, into the biological functions of specific enzymes of the Kennedy pathway. We discuss potential mechanisms of how mutations within the same pathway can cause disparate disease. 相似文献
109.
Diel water movement between parenchyma and chlorenchyma of two desert CAM plants under dry and wet conditions 总被引:3,自引:2,他引:3
Abstract. Electric-circuit analogue models of the water relations of crassulacean acid metabolism (CAM) succulents such as Agave deserti and Ferocactus acanthodes have predicted diel movement of water between the water-storage parenchyma and the photo-synthetic chlorenchyma. Injection of tritiated water into either tissue in the laboratory confirmed substantial and bidirectional water movements, especially under conditions of wet soil. For A. deserti , water movement from the water-storage parenchyma to the chlorenchyma increased at night as the chlorenchyma osmotic pressure increased. Although nocturnal osmotic pressure increases and transpiration for both species were minimal in the field under dry conditions, diel changes in the deuterium: hydrogen ratio (expressed as ΔD) were similar for the water-storage parenchyma and the chlorenchyma. Such indication of [substantial mixing of water between the tissues over a 24-h cycle was more evident under wet conditions in the field. For A. deserti , ΔD then increased by 32%o from the afternoon to midnight and was essentially identical in the water-storage parenchyma and the chlorenchyma. For F. acanthodes , the diel changes in ΔD were one-third those of A. deserti , and ΔD was always slightly higher for the chlorenchyma than for the water-storage parenchyma, apparently reflecting the lower surface-to-volume ratio of A. deserti. In summary, data obtained using radioactive and stable isotopes strongly supported model predictions concerning diel cycles of internal water distribution for these CAM species. 相似文献
110.
Gill morphometrics of the thresher sharks (Genus Alopias): Correlation of gill dimensions with aerobic demand and environmental oxygen 下载免费PDF全文
Gill morphometrics of the three thresher shark species (genus Alopias) were determined to examine how metabolism and habitat correlate with respiratory specialization for increased gas exchange. Thresher sharks have large gill surface areas, short water–blood barrier distances, and thin lamellae. Their large gill areas are derived from long total filament lengths and large lamellae, a morphometric configuration documented for other active elasmobranchs (i.e., lamnid sharks, Lamnidae) that augments respiratory surface area while limiting increases in branchial resistance to ventilatory flow. The bigeye thresher, Alopias superciliosus, which can experience prolonged exposure to hypoxia during diel vertical migrations, has the largest gill surface area documented for any elasmobranch species studied to date. The pelagic thresher shark, A. pelagicus, a warm‐water epi‐pelagic species, has a gill surface area comparable to that of the common thresher shark, A. vulpinus, despite the latter's expected higher aerobic requirements associated with regional endothermy. In addition, A. vulpinus has a significantly longer water–blood barrier distance than A. pelagicus and A. superciliosus, which likely reflects its cold, well‐oxygenated habitat relative to the two other Alopias species. In fast‐swimming fishes (such as A. vulpinus and A. pelagicus) cranial streamlining may impose morphological constraints on gill size. However, such constraints may be relaxed in hypoxia‐dwelling species (such as A. superciliosus) that are likely less dependent on streamlining and can therefore accommodate larger branchial chambers and gills. J. Morphol. 276:589–600, 2015. © 2015 Wiley Periodicals, Inc. 相似文献