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91.
《Plant Ecology & Diversity》2013,6(2):217-229
Background: Hybridisation in Senecio/Jacobaea is frequent and of significance in speciation and evolution. Moreover, these genera are model systems for studying the evolution of pyrrolizidine alkaloids (PAs). Hybrids between Jacobaea aquatica and J. vulgaris have been reported from north-western Europe. Aims: Our aim was to determine whether hybrids between Jacobaea aquatica and J. vulgaris also occurred in Austria, and if these hybrids could be responsible for the presumed increase of J. aquatica-like plants in this part of Europe. Furthermore, we tested if such hybrids showed an altered PA spectrum compared with parental species. Methods: We used amplified fragment length polymorphisms (AFLPs) to analyse genetic diversity and structure, and gas chromatography–mass spectrometry (GC–MS) to identify PAs. Results: We were able to identify genetically one hybrid population adjacent to a large J. aquatica population. The AFLP phenotypes of hybrids demonstrated a closer relationship to J. aquatica, suggesting frequent backcrossing. All hybrid individuals contained the same set of PAs as the parental species, but in differing amounts, plus four additional PAs, one of which may be novel. Conclusions: Although hybridisation in Jacobaea is common, we were able to confirm only one hybrid population in Austria. Therefore, it seems unlikely that hybridisation plays a role in the presumed increase of J. aquatica in this area. 相似文献
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Neural progenitor cells have a central role in the development and evolution of the vertebrate brain. During early brain development, neural progenitors first expand their numbers through repeated proliferative divisions and then begin to exhibit neurogenic divisions. The transparent and experimentally accessible optic tectum of Xenopus laevis is an excellent model system for the study of the cell biology of neurogenesis, but the precise spatial and temporal relationship between proliferative and neurogenic progenitors has not been explored in this system. Here we construct a spatial map of proliferative and neurogenic divisions through lineage tracing of individual progenitors and their progeny. We find a clear spatial separation of proliferative and neurogenic progenitors along the anterior‐posterior axis of the optic tectum, with proliferative progenitors located more posteriorly and neurogenic progenitors located more anteriorly. Since individual progenitors are repositioned toward more anterior locations as they mature, this spatial separation likely reflects an increasing restriction in the proliferative potential of individual progenitors. We then examined whether the transition from proliferative to neurogenic behavior correlates with cellular properties that have previously been implicated in regulating neurogenesis onset. Our data reveal that the transition from proliferation to neurogenesis is associated with a small change in cleavage plane orientation and a more pronounced change in cell cycle kinetics in a manner reminiscent of observations from mammalian systems. Our findings highlight the potential to use the optic tectum of Xenopus laevis as an accessible system for the study of the cell biology of neurogenesis. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 76: 1328–1341, 2016 相似文献
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The foundational concept for our research, which is largely shared by statisticians and ecologists, is that biodiversity is one of the most important indicators for environmental assessment. Because this indicator decreases in relation to ecosystem stressors, its measurement is essential for predicting future biological impacts of environmental damages. Although many indices have been proposed, no universally accepted measure for biodiversity has yet been established. In this context, the use of diversity profiles allows the analyst to display a family of indices in a single graph. However, this approach presents two critical limitations: first, a community composition is not always interpretable; second, the diversity profiles could lead to ranking issues when the curves intersect each other. The aim of this paper is to resolve these limitations by introducing functional biodiversity tools. In particular, three functional measures are proposed: the derivatives, the radius of curvature and the curve length. The analysis of derivatives and of the radius of curvature addresses the first limitation and highlights the characteristics, the differences and the similarities among communities. Arc length addresses the second limitation, providing a scalar measure that leads to a unique communities ranking for a given pattern of richness even if profiles intersect. The proposed functional models are applied to a real data set involving lichen biodiversity in the province of Genoa, Italy. Our approach allowed us to analyze the characteristics of lichen communities and to identify the biodiversity ranking. The combined use of these tools provides a useful method for identifying areas of high environmental risk, with the potential to address the monitoring of environmental policies. 相似文献
98.
目的:比较经腹、经阴道与经会阴三种超声途径测量宫颈长度对早产的预测价值。方法:选取我院就诊的孕妇780例,根据测量途径不同分为三组,每组260例,分别采用经腹、经阴道与经会阴方法测量宫颈长度。观察并比较三组患者的妊娠结局,以及三种测量方法的敏感度及特异性。结果:三组患者接受率相比,经腹组、经会阴组患者接受率明显高于经阴道组(P0.05);三组患者宫颈显示率相比,经会阴组、经阴道组宫颈显示率显著高于经腹组(P0.05)。经腹组中,宫颈长度2.5 cm的患者早产率为48.46%,明显高于宫颈长度≥2.5 cm的患者早产率17.69%(P0.05);经会阴组中,宫颈长度2.5 cm的患者早产率为37.69%,明显高于宫颈长度≥2.5 cm的患者早产率13.08%(P0.05);经阴道组中,宫颈长度2.5 cm的患者早产率为36.92%,明显高于宫颈长度≥2.5 cm的患者早产率13.46%(P0.05)。结论:三种超声途径测量宫颈长度对预测早产均有很大的临床价值,其中经会阴途径优势更大,值得进一步推广临床使用。 相似文献
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MyROOT: a method and software for the semiautomatic measurement of primary root length in Arabidopsis seedlings 总被引:1,自引:0,他引:1
Isabel Betegn‐Putze Alejandro Gonzlez Xavier Sevillano David Blasco‐Escmez Ana I. Cao‐Delgado 《The Plant journal : for cell and molecular biology》2019,98(6):1145-1156
Root analysis is essential for both academic and agricultural research. Despite the great advances in root phenotyping and imaging, calculating root length is still performed manually and involves considerable amounts of labor and time. To overcome these limitations, we developed MyROOT, a software for the semiautomatic quantification of root growth of seedlings growing directly on agar plates. Our method automatically determines the scale from the image of the plate, and subsequently measures the root length of the individual plants. To this aim, MyROOT combines a bottom‐up root tracking approach with a hypocotyl detection algorithm. At the same time as providing accurate root measurements, MyROOT also significantly minimizes the user intervention required during the process. Using Arabidopsis, we tested MyROOT with seedlings from different growth stages and experimental conditions. When comparing the data obtained from this software with that of manual root measurements, we found a high correlation between both methods (R2 = 0.997). When compared with previous developed software with similar features (BRAT and EZ‐Rhizo), MyROOT offered an improved accuracy for root length measurements. Therefore, MyROOT will be of great use to the plant science community by permitting high‐throughput root length measurements while saving both labor and time. 相似文献
100.
Gonzalez-Voyer A Székely T Drummond H 《Evolution; international journal of organic evolution》2007,61(8):1946-1955
In many parentally fed species, siblings compete for food not only by begging and scrambling, but also by violently attacking each other. This aggressive competition has mostly been studied in birds, where it is often combined with dominance subordination, aggressive intimidation, and siblicide. Previous experimental and theoretical studies proposed several life-history, morphological, and behavioral variables that may facilitate the evolution of broodmate aggression, and explain its taxonomic distribution. Here we apply phylogenetic comparative analyses for the first time to test the influence of five hypothesized facilitators of the evolution of broodmate aggression, analyzing 69 species in seven avian families using two quantitative measures of aggression: incidence and intensity. We show that incidence and intensity of aggression increase with long nestling periods and indirect feeding, and small brood size is associated with intense aggression. Large food parcels were not correlated with either the incidence or intensity of aggression. Our study suggests that indirect feeding, long nestling periods, and small broods, possibly in combination with other factors, have tended to favor the evolution of aggressive broodmate competition. 相似文献