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11.
I Gallien F Caurant M Bordes P Bustamante P Miramand B Fernandez N Quellard P Babin 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2001,130(3):389-395
Top predators from the northern sub-polar and polar areas exhibit high cadmium concentrations in their tissues. In the aim to reveal possible adverse effects, samples of five Atlantic white-sided dolphins Lagenorhyncusacutus have been collected on the occasion of the drive fishery in the Faroe Islands, for ultrastructural investigations and energy dispersive X-ray microanalyses. Cadmium concentrations were less than the limit of detection in both immature individuals and ranged from 22.7 to 31.1 μg g?1 wet weight in the mature individuals. Two individuals with the highest cadmium concentrations exhibited electron dense mineral concretions in the basal membranes of the proximal tubules. They are spherocrystals made up of numerous strata mineral deposit of calcium and phosphorus together with cadmium. Cadmium has been detected with a molar ratio of Ca:Cd of 10:1 in the middle of these concretions. To our knowledge, this is the first report of such granules in a wild vertebrate. The role of these granules in the detoxification of the metal and the possible pathological effects are considered. 相似文献
12.
Trypsin-releasable glycosaminoglycans from the luminal surface of intact pig aorta were measured following metabolic labelling with35S]sulphate. Chondroitin sulphate was found to be present at a surface density equal to that already established for heparan sulphate (5×1011 chains per cm2). This result was confirmed by X-ray microanalysis of the luminal sulphur content before and after treatment with specific glycosaminoglycan-degrading enzymes. This result implies that approximately half of the luminal surface is occupied by sulphated glycosaminoglycans. 相似文献
13.
Barry M. Gordon 《Biological trace element research》1987,12(1):153-159
Information concerning the chemical state of trace elements in biological systems generally has not been available. Such information
for toxic elements and metals in metalloproteins could prove extremely valuable in the elucidation of their metabolism and
other biological processes. The shielding of core electrons by binding electrons affect the energy required for creating inner-shell
holes. Furthermore, the molecular binding and symmetry of the local environment of an atom affect the absorption spectrum
in the neighborhood of the absorption edge. X-ray absorption near-edge structure (XANES) using synchrotron radiation excitation
can be used to provide chemical speciation information for trace elements at concentrations as low as 10 ppm. The structure
and position of the absorption curve in the region of an edge can yield vital data about the local structure and oxidation
state of the trace element in question. Data are most easily interpreted by comparing the observed edge structure and position
with those of model compounds of the element covering the entire range of possible oxidation states. Examples of such analyses
will be reviewed. 相似文献
14.
Many biomedical experiments require the qualitative and quantitative localization of trace elements with high sensitivity
and good spatial resolution. The feasibility of measuring the chemical form of the elements, the time course of trace element
metabolism, and conducting experiments in living biological systems are also important requirements for biological trace element
research. Nuclear analytical techniques that employ ion or photon beams have grown in importance in the past decade and have
led to several new experimental approaches. Some of the important features of these methods are reviewed here along with their
role in trace element research. Examples of their use are given to illustrate potential for new research directions. It is
emphasized that the effective application of these methods necessitates a closely integrated multidisciplinary scientific
team. 相似文献
15.
A particle-induced X-ray emission (PIXE) analysis method is presented, which allows measurement of eight elements (i.e., K,
Ca, Mn, Fe, Cu, Zn, Se, and Rb) in human brain samples of only a few mg dry weight. The precision and accuracy of the method
were investigated by analyzing animal brain matter with both PIXE and instrumental neutron activation analysis (INAA). The
method was applied to measure the 8 elements in 46 different regions of 3 human brains. The sections analyzed originated from
either the left or the right cerebral hemisphere, brain stem, and cerebellum. For one of the brains, sections were also analyzed
from 26 corresponding regions of both hemispheres. For all elements, similar concentrations were found in the corresponding
areas of the left and right sides of the brain. The concentrations (in μg/g dry weight) of the elements K, Fe, Cu, Zn, Se,
and Rb were consistently higher in cortical structures than in white matter. Deep nuclei and brain stem, which have a mixed
composition, showed intermediate values for K, Zn, Se, and Rb. A hierarchical cluster analysis indicated that the various
brain regions clustered into two large groups, one comprising gray and mixed matter regions and the other, white and mixed
matter brain areas. 相似文献
16.
P. M. Abuja M. Schmuck I. Pilz P. Tomme M. Claeyssens H. Esterbauer 《European biophysics journal : EBJ》1988,15(6):339-342
Limited proteolysis (papain) of the cellobiohydrolase I (CBH I, 65 kDa) from Trichoderma reesei led to the seperation of two functional domains: a core protein (55 kDa) containing the active site, and a C-terminal glycopeptide (10 kDa) implicated in binding to the insoluble matrix (cellulose). The quaternary structures of the intact CBH I and its core in solution are now compared by small angle X-ray scattering (SAXS) measurements. The molecular parameters derived for the core (Rg=2.09 nm, Dmax=6.5 nm) and for the intact enzyme (Rg=4.27 nm, Dmax=18 nm) indicate very different shapes. The resulting models show a tadpole-like structure for the intact enzyme where the isotropic part coincides with the core protein and the flexible tail part should be identified with the C-terminal glycopeptide. Thus in this enzyme, functional differentiation is reflected in structural peculiarities.Abbreviations SAXS
small angle X-ray scattering
- SDS-PAGE
SDS-polyacrylamide gel electrophoresis
- IEF-PAG
polyacrylamide gel isoelectric focusing; cellobiohydrolase (CBH, 1,4--glucan cellobio hydrolase (E.C.3.2.1.91))
- Dmax
maximum diameter
- Rg
radius of gyration 相似文献
17.
Summary Tree-ring data of naturally grown connifers were analyzed to evaluate the possibility of enhanced tree growth due to increased atmospheric CO2. Tree cores were obtained from 34 sites in four different climatic regions in the northern hemisphere. In each of the four regions, the sampling sites were located along ecological gradients between the subalpine treeline and low elevations and, sometimes, the arid forest border. Growth trends after 1950, when the atmospheric CO2 concentration increased by more than 30 l·l-1 indicate an increase in ring-widths at eight of the 34 sites. These chronologies were from sites which moderate temperature or water stress. In four cases the growth increase in the post-1950 period coincided with favorable climatic conditions. In the remaining four cases, the growth increase exceeded the upper bound response expected from CO2 enrichment experiments with seedling conifer species. Therefore, increased growth in any of the tree-ring chronologies examined could not be solely attributed to higher atmospheric CO2 concentrations.Major financial supporters: Swiss National Science Foundation (application no. 1.869-0.83); Swiss Federal Institute of Forestry Research, 8903 Birmensdorf, Switzerland; other financial supporters: Carbon Dioxide Research Division, U.S. Department of Energy under subcontract no. 11X-57507V with Martin Marietta Energy Systems, IncOperated by Martin Marietta Energy Systems, Inc., under contract DE-AC05-840R21400 with U.S. Department of Energy 相似文献
18.
The three-dimensional structure of the human immunoglobulin fragment Fab New (IgG1, lambda) has been refined to a crystallographic R-factor of 16.9% to 2 A resolution. Rms deviations of the final model from ideal geometry are 0.014 A for bond distances and 3.03 degrees for bond angles. Refinement was based on a new X-ray data set including 28,301 reflections with F > 2.5 sigma(F) from 6.0 to 2.0 A resolution. The starting model for the refinement procedure reported here is from the Brookhaven Protein Data Bank entry 3FAB (rev. 1981). Differences between the initial and final models include modified polypeptide-chain folding in the third complementarity-determining region (CDR3) and the third framework region (FR3) of VH and in some exposed loops of CL and CH1. Amino acid sequence changes were determined at a number of positions by inspection of difference electron density maps. The incorporation of amino acid sequence changes results in an improved VH framework model for the "humanization" of monoclonal antibodies. 相似文献
19.
The packing of peptide helices in crystals of the leucine-rich decapeptide Boc-Aib-Leu-Aib-Aib-Leu-Leu-Leu-Aib-Leu-Aib-OMe provides an example of ladder-like leucylleucyl interactions between neighboring molecules. The peptide molecule forms a helix with five 5----1 hydrogen bonds and two 4----1 hydrogen bonds near the C terminus. Three head-to-tail NH ... O = C hydrogen bonds between helices form continuous columns of helices in the crystal. The helicial columns associate in an antiparallel fashion, except for the association of Leu ... Leu side chains, which occurs along the diagonal of the cell where the peptide helices are parallel. The peptide, with formula C56H102N10O13, crystallizes in space group P2(1)2(1)2(1) with Z = 4 and cell parameters a = 16.774(3) A, b = 20.032(3) A and c = 20.117(3) A; overall agreement factor R = 10.7% for 2014 data with magnitude of F(obs) greater than 3 sigma (F); resolution 1.0 A. 相似文献
20.
Summary The ectomycorrhizal fungus,Paxillus involutus, produces sclerotia in culture. These can be induced to form on agar medium by exposing mycelium grown at 25°C to various temperatures between6°C and 15°C. Sclerotia formed at 10°C and above were large and covered with drops of exudate, while those formed at 6°C or 8°C were very small and did not produce an exudate. Mature sclerotia were bounded by a compact rind and contained abundant storage reserves. Histochemistry of the larger sclerotia showed large quantities of protein stored as protein bodies in the cytoplasm, lipid present as small droplets, glycogen granules stored in the cytoplasm and polyphosphate present as small granules in the cytoplasm and in the protein bodies. Energy dispersive X-ray microanalysis confirmed the presence of phosphate in the granules and was used to map its distribution throughout the sclerotium. The smaller sclerotia induced at 8°C and below on the same medium had the same basic structure and composition, but lacked the complex phenolic cell network found in large sclerotia, and had abundant extracellular polysaccharides. The rind was not well developed and these small sclerotia are interpreted to have been arrested at an early stage of development. 相似文献