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51.
Kuttanpillai Santhosh Kumar Kugen Permaul Suren Singh 《Engineering in Life Science》2009,9(4):298-302
Ten strains of Thermomyces lanuginosus from various culture collections were evaluated for extracellular endo‐β‐1,4‐xylanase production. The best xylanase producer (5771±173 nkat/mL) T. lanuginosus SK, was subjected to UV and N‐methyl‐N‐nitro‐N‐nitrosoguanidine mutagenesis. A mutant strain T. lanuginosus MC134, that showed on oatspelts xylan a 1.5 fold higher xylanase production than the parent strain SK, was subjected to a study of the regulation of xylanase synthesis during growth on various carbohydrates and during induction in glucose‐grown cells. In the growth experiments the highest production of xylanase was observed in the presence of xylans, however, an appreciable amount of the enzyme, about 10%, was also produced during growth on xylose. Xylobiose was found to be the most efficient xylanase inducer in the glucose‐grown cells. Its induction efficiency was followed by xylose, beechwood and birchwood xylan. Xylanase induction by polysaccharides started several hours later but proceeded for a longer time than that induced by the low molecular mass inducers, indicating that the polysaccharides serve as more sustainable source of inducers and that they have to be first hydrolyzed by the low level of constitutively synthesized xylanase. The repression of the induction of xylanase by glucose confirmed that the xylanase synthesis in the mutant strain is similar to the parent strain and exhibits an induction‐repression regulation mechanism. 相似文献
52.
Maximal pectinolytic activity was detected in the culture filtrates of Thermomyces lanuginosus when grown in medium containing pectin and sucrose. The pectinolytic enzyme system was optimally active at pH 5.5 and at 70°C with potassium pectate and at pH 4.5 at 50°C with pectin as substrates. Zymogram analyses showed two activity bands with pectin and three with potassium pectate. 相似文献
53.
Substrate recognition of Thermomyces lanuginosus lipase in the acylation of nucleosides was revealed through rational substrate engineering for the first time. T. lanuginosus lipase displayed higher catalytic activities and excellent 5′-regioselectivities (94–>99%) in the acylation of ribonucleosides 1f–1j as compared to those in the acylation of 2′-deoxynucleosides 1a–1e. The higher reaction rates and excellent 5′-regioselectivities might derive from a favorable hydrogen bonding between the 2′-hydroxyl group of 1f–1j and phenolic hydroxyl group of Tyr21 present in the hydrophilic region of the lipase. 相似文献
54.
Z. Q. Jiang S. Q. Yang Q. J. Yan L. T. Li S. S. Tan 《World journal of microbiology & biotechnology》2005,21(6-7):863-867
Summary Thermomyces lanuginosus CAU44, a newly isolated thermophilic fungus strain, was used for the production of extracellular xylanase on various lignocellulosic
materials under shake flask conditions. High-level production of xylanase by the strain was enhanced by optimizing the type
of carbon sources, substrate concentration, particle size and surfactants in the culture medium. The titre of xylanase activity
obtained of up to 4156 U ml−1 was the highest ever reported. 相似文献