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51.
Nigel R. Andrew Robert A. Hart Myung-Pyo Jung Zac Hemmings John S. Terblanche 《Journal of insect physiology》2013
Insects in temperate regions are predicted to be at low risk of climate change relative to tropical species. However, these assumptions have generally been poorly examined in all regions, and such forecasting fails to account for microclimatic variation and behavioural optimisation. Here, we test how a population of the dominant ant species, Iridomyrmex purpureus, from temperate Australia responds to thermal stress. We show that ants regularly forage for short periods (minutes) at soil temperatures well above their upper thermal limits (upper lethal temperature = 45.8 ± 1.3 °C; CTmax = 46.1 °C) determined over slightly longer periods (hours) and do not show any signs of a classic thermal performance curve in voluntary locomotion across soil surface temperatures of 18.6–57°C (equating to a body temperature of 24.5–43.1 °C). Although ants were present all year round, and dynamically altered several aspects of their thermal biology to cope with low temperatures and seasonal variation, temperature-dependence of running speed remained invariant and ants were unable to elevate high temperature tolerance using plastic responses. Measurements of microclimate temperature were higher than ant body temperatures during the hottest part of the day, but exhibited a stronger relationship with each other than air temperatures from the closest weather station. Generally close associations of ant activity and performance with microclimatic conditions, possibly to maximise foraging times, suggest I. purpureus displays highly opportunistic thermal responses and readily adjusts behaviour to cope with high trail temperatures. Increasing frequency or duration of high temperatures is therefore likely to result in an immediate reduction in foraging efficiency. In summary, these results suggest that (1) soil-dwelling temperate insect populations may be at higher risks of thermal stress with increased frequency or duration of high temperatures resulting from climate change than previously thought, however, behavioural cues may be able to compensate to some extent; and (2) indices of climate change-related thermal stress, warming tolerance and thermal safety margin, are strongly influenced by the scale of climate metrics employed. 相似文献
52.
The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events in cryoprotective cocktails containing synthetic ice modulators (SIM)—compounds which hinder ice crystal growth. Cryopreservation by vitrification is a highly complex application, where the likelihood of crystallization, fracture formation, degradation of the biomaterial quality, and other physical events are dependent not only upon the instantaneous cryogenic conditions, but more significantly upon the evolution of conditions along the cryogenic protocol. Nevertheless, cryopreservation success is most frequently assessed by evaluating the cryopreserved product at its end states—either at the cryogenic storage temperature or room temperature. The cryomacroscope is the only available device for visualization of large-size specimens along the thermal protocol, in an effort to correlate the quality of the cryopreserved product with physical events. Compared with earlier cryomacroscope prototypes, the new Cryomacroscope-III evaluated here benefits from a higher resolution color camera, improved illumination, digital recording capabilities, and high repeatability in tested thermal conditions via a commercial controlled-rate cooler. A specialized software package was developed in the current study, having two modes of operation: (a) experimentation mode to control the operation of the camera, record camera frames sequentially, log thermal data from sensors, and save case-specific information; and (b) post-processing mode to generate a compact file integrating images, elapsed time, and thermal data for each experiment. The benefits of the Cryomacroscope-III are demonstrated using various tested mixtures of SIMs with the cryoprotective cocktail DP6, which were found effective in preventing ice growth, even at significantly subcritical cooling rates with reference to the pure DP6. 相似文献
53.
Hidehito Urata Shuji Ogawa Shun-ichi Wada 《Bioorganic & medicinal chemistry letters》2013,23(10):2909-2911
The effects of substituting l-deoxynucleotide for d-deoxynucleotide at duplex termini were evaluated and the terminal substitutions were found to show much less effects on duplex destabilization and to show a similar tendency in base pairing selectivity, compared with internal chiral substitutions. 相似文献
54.
Xiao-long Tang Feng Yue Jian-zheng He Ning-bo Wang Ming Ma Jia-run Mo Qiang Chen 《Zoology (Jena, Germany)》2013,116(6):331-335
A viviparous lizard, Eremias multiocellata, was used to investigate the possible sexual and ontogenetic effects on selected body temperature, thermal tolerance range and the thermal dependence of locomotor performance. We show that adults are sexually dimorphic and males have larger bodies and heads than females. Adults selected higher body temperatures (34.5 vs. 32.4 °C) and could tolerate a broader range of body temperatures (8.1–46.8 vs. 9.1–43.1 °C) than juveniles. The sprint speed and maximum sprint distance increased with temperature from 21 °C to 33 °C, but decreased at 36 °C and 39 °C in both juveniles and adults. Adults ran faster and longer than juveniles at each tested temperature. Adult locomotor performance was not correlated with snout–vent length (SVL) or sex, and sprint speed was positively correlated with hindlimb length. Juvenile locomotor performance was positively correlated with both SVL and hindlimb length. The ontogenetic variation in selected body temperature, thermal tolerance and locomotor performance in E. multiocellata suggests that the effects of morphology on temperature selection and locomotor performance vary at different ontogenetic stages. 相似文献
55.
为了解2013-2014年湖北省外环境中禽流感病毒污染情况,采用实时荧光定量聚合酶链反应(polymerase chain reaction,PCR)对全省7个监测点1 642份活禽相关外环境标本进行禽流感病毒型别与阳性率监测.结果显示,2013-2014年湖北省外环境中禽流感病毒平均阳性检出率为19.1%,检出的禽流感病毒型别以H9亚型为主(占57.3%),未检出H7亚型.不同地区和季节禽流感病毒阳性率有所差异,鄂西北冬春季病毒检出阳性率高于鄂西南和江汉平原;家禽屠宰加工厂检出阳性率最高;阳性率最高的标本来自清洗禽类的污水.结果提示,湖北地区外环境中禽流感病毒阳性率较高,且型别多样,家禽屠宰加工人员对禽流感病毒有较高的暴露风险,需加强健康宣教,提高个人防护意识. 相似文献
56.
《Harmful algae》2016
Dinophysis acuta is a frequent seasonal lipophilic toxin producer in European Atlantic coastal waters associated with thermal stratification. In the Galician Rías, populations of D. acuta with their epicentre located off Aveiro (northern Portugal), typically co-occur with and follow those of Dinophysis acuminata during the upwelling transition (early autumn) as a result of longshore transport. During hotter than average summers, D. acuta blooms also occur in August in the Rías, when they replace D. acuminata. Here we examined a 30-year (1985–2014) time series of D. acuta from samples collected by the same method in the Galician Rías. Our main objective was to identify patterns of distribution and their relation with climate variability, and to explain the exceptional summer blooms of D. acuta in 1989–1990. A dome-shaped relationship was found between summer upwelling intensity and D. acuta blooms; cell maxima were associated with conditions where the balance between upwelling intensity and heating, leading to deepened thermoclines, combined with tidal phase (3 days after neap tides) created windows of opportunity for this species. The application of a generalized additive model based on biological (D. acuta inoculum) and environmental predictors (Cumulative June–August upwelling CUIJJA, average June–August SSTJJA and tidal range) explained more than 70% of the deviance for the exceptional summer blooms of D. acuta, through a combination of moderate (35,000–50,000 m3 s−1 km−1) summer upwelling (CUIJJA), thermal stratification (SSTJJA > 17 °C) and moderate tidal range (∼2.5 m), provided D. acuta cells (inoculum) were present in July. There was no evidence of increasing trends in D. acuta bloom frequency/intensity nor a clear relationship with NAO or other long-term climatic cycles. Instead, the exceptional summer blooms of 1989–1990 appeared linked to extreme hydroclimatic anomalies (high positive anomalies in SST and NAO index), which affected most of the European Atlantic coast. 相似文献
57.
The effects of increased temperature were tested in Amphiprion ocellaris, using a cellular diagnostics approach (in several tissues) combined with an organismal approach (body condition). Clownfish were exposed to a one month experiment following two temperature treatments: control (26 °C) and elevated temperature (30 °C). Fish were sampled at 0, 7, 14, 21 and 28 days for (1) assessment of stress biomarkers (catalase, lipid peroxidation, glutathione-S-transferase, superoxide dismutase, acetylcholinesterase, heat shock protein 70 kDa and ubiquitin – in brain, gills, liver, intestine and muscle), (2) estimation of integrated biomarker response index based on the biomarkers tested and (3) assessment of Fulton’s K index. Results show all biomarkers except acetylcholinesterase responded consistently and significantly to elevated temperature across tissue types suggesting they are suitable indicators of thermal stress in A. ocellaris. Biomarker levels were tissue-specific, and in addition, the most reactive tissues to temperature were muscle, gills and liver which suggest that highly oxygenated tissues seem to be the most responsive under thermal stress. The most responsive sampling times to increased temperature were T7 and T28: thermal stress was observed after 7 days of exposure (biomarker levels increased), then a pattern of decrease in biomarker levels towards the end of the experiment was observed, which may suggest fish were able to acclimate to exposure conditions. This indicates that A. ocellaris probably lives far from its upper thermal limit and is capable of adjusting the protein quality control system and enzymes’ activities to protect cell functions under elevated temperatures. The temperature treatment did not significantly influence body condition of the animals but biomarkers were negatively correlated to wet body weight. This suggests that thermal acclimation incurs at some energetic cost. In conclusion, these results suggest that this coral reef fish species presents a significant acclimation potential under ocean warming scenarios of +4 °C. 相似文献
58.
The large volume of water in the Pará River, together with governmental incentives, has attracted many industries to the city of Barcarena, Brazil. This industrial activity has the potential to cause changes to aquatic environments. Zooplankton species are considered good indicators of environmental changes. We assessed the association between changes in community composition and proximity to an industrial-port complex, and identified potential bioindicator species in these environments. Five quarterly sampling points were selected along the Pará River (P1–P5) in 2012. The zooplankton community in this region is composed of 64 species. The highest total densities were recorded in February and November, which are both during the rainy season. Zooplankton density was greatest at P3, which was near an industrial-port complex, suggesting that industrial activity affected zooplankton density. An IndVal test showed the rotifer Filinia opoliensis (r = 0.86, p = 0.02) to be a possible bioindicator of environmental quality in the study area. This paper contributes to the discussion of the impacts of installing industrial plants and large ports in the Amazon. 相似文献
59.
To investigate the pollution status and potential pollution risk of mercury (Hg) in China, surface sediment samples were collected from eight hundred and eighty-one sites, including ten major basins (Songhua River Basin (SRB), Liao River Basin (LRB), Hai River Basin (HRB), Yellow River Basin (YRB), Huai River Basin (HuRB), Yangtze River Basin (YtRB), Pearl River Basin (PRB), Southeastern River Basin (SeRB), Southwestern River Basin (SwRB) and Northwestern River Basin (NwRB)). Results showed that Hg concentrations in sediments of ten basins in China ranged from 0.001 to 8.800 mg/kg, with average ± S.D. value of 0.274 ± 0.675 mg/kg, which was obviously higher than Chinese soil background value (0.038 mg/kg) and Chinese sediment background value (0.040 mg/kg). The mean Hg concentration of ten basins decreased in the order of HRB > YtRB > SRB > PRB > HuRB > SwRB > YRB > SeRB > LRB > NwRB. Moreover, it was found that the Hg concentrations in the sediments of LRB, YtRB, PRB, SeRB and SwRB were partly driven by their total organic carbon (TOC) contents, while the effect of pH on the distribution of Hg was not obvious. The Hg concentration data were also compared with those got in other periods (1994–2015) to obtain the general variation tendency of Hg level. It was recorded that Hg concentrations in HRB have remained on high levels for a long history, while Hg contamination situation in YRB after 2004 has potentially turned to be better. The results of pollution assessment by sediment quality assessment guidelines (SQGs), contamination factor (CF), geoaccumulation index (Igeo) and potential ecological risk (Ei) suggested that YRB and HRB were the most seriously polluted river basins among the ten basins. It is urgent of constructing SQGs in China to scientifically evaluate the Hg pollution in the future. 相似文献
60.
Increasing economic growth and industrial development in China is starting to impact even remote areas such as the Shennongjia nature reserve, where nitrogen pollution is becoming a major environmental threat. The epiphytic lichen flora is particularly rich in this area and is one of the components of this habitat most sensitive to nitrogen pollution. Since lichens represent an important food resource for the endangered monkey species Rhinopithecus roxellana, a reduction in lichen availability would have harmful consequences for the conservation of its habitat in the Shennongjia Mountains. To investigate the effects of increased nitrogen availability on the local lichen communities, so far scarcely considered, we conducted a one-year field experiment measuring growth, survival, and phosphomonoesterase activity of the widespread species Usnea luridorufa in response to nitrogen (up to 50 kg N ha−1 year−1 deposition) and phosphorus supply. Growth and survival of thalli and propagules of U. luridorufa decreased when treated with N deposition >12.05 kg N ha−1 year−1 and >2.14 kg N ha−1 year−1, respectively. The important role of phosphorus availability in relation to nitrogen supply was demonstrated by the increase in phosphomonoesterase activity with increasing nitrogen availability until a nitrogen toxicity threshold was reached. However, the high concentration of phosphorus in rainwater showed that phosphorus is not a limiting nutrient in the area.The results make a contribution to the knowledge of the negative effects of increased N deposition in the Shennongjia forest ecosystem. 相似文献